Reversible G(1) arrest by dimethyl sulfoxide as a new method to synchronize Chinese hamster cells.

abstract：:In order to establish the capacity of Mytilus spp. to form genotoxin-DNA adducts, a series of in vitro and in vivo studies were conducted in which tissue samples and animals were exposed to five model genotoxins. Following the in vitro characterization of the major adducts induced by the compounds, a series of in vivo...

journal_title：Mutagenesis

authors： Harvey JS,Parry JM

更新日期：1997-05-01 00:00:00

abstract：:A substantial database on hprt mutant frequency in circulating T-cells derived from 153 normal humans was analysed to determine if any effect of alcohol consumption could be detected. Alcohol intake (as reported in a questionnaire completed at the time of the blood sample) ranged from 0 to 35+ units per week. Donors w...

journal_title：Mutagenesis

authors： Cole J,Green MH

更新日期：1995-09-01 00:00:00

abstract：:The spectrum of mutations induced by cobalt(II) chloride (CoCl2) was examined using plasmid pUB3 DNA, which was propagated after transfection into Escherichia coli SY1032/pKY241 host cells. The vector plasmid carried an E.coli supF suppressor tRNA gene as a target for mutations. After CoCl2 treatment, 64 independent n...

journal_title：Mutagenesis

authors： Ogawa HI,Ohyama Y,Ohsumi Y,Kakimoto K,Kato Y,Shirai Y,Nunoshiba T,Yamamoto K

更新日期：1999-03-01 00:00:00

abstract：:Cyclooxygenase 2 (Cox-2, a rate-limiting enzyme in the conversion of arachidonic acid to prostanoids) has been implicated in several physiological and pathological processes, and it has been reported that polymorphisms in the regulatory region of Cox-2 might influence its expression, contributing to the interindividua...

journal_title：Mutagenesis

authors： Tang Z,Nie ZL,Pan Y,Zhang L,Gao L,Zhang Q,Qu L,He B,Song G,Zhang Y,Shukui Wang

更新日期：2011-11-01 00:00:00

abstract：:The major risk factor for skin cancer is exposure to UV radiation from sunlight, but other environmental exposures may also play a role in combination with UV. We have studied the effects of combined exposure of primary human skin cells in vitro to UVA, UVB or UVC with benzo[a] pyrene (BaP), an environmental carcinoge...

journal_title：Mutagenesis

authors： Crallan RA,Ingham E,Routledge MN

更新日期：2005-07-01 00:00:00

abstract：:3 beta-Hydroxy-13 alpha-amino-13,17-seco-5 alpha-androstan-17-oic-13,17-lactam-p-bis(2-chloroethyl) aminophenoxyacetate (NSC 294859) is a new modified steroidal alkylating agent. This compound was given by i.p. administration to mice bearing different types of tumour. It was found to exhibit good activity in L1210 and...

journal_title：Mutagenesis

authors： Tselepi MR,Demopoulos NA,Catsoulacos P

更新日期：1989-09-01 00:00:00

abstract：:In order to validate the in vivo micronucleus test in mouse splenocytes using the cytokinesis block method, 14 compounds with various mechanisms of action were tested: three direct alkylating agents (mitomycin C, ethylnitrosourea, beta-propiolactone), seven indirect alkylating agents (cyclophosphamide, benzo[a]pyrene,...

journal_title：Mutagenesis

authors： Benning V,Brault D,Duvinage C,Thybaud V,Melcion C

更新日期：1994-05-01 00:00:00

abstract：:The Ser326Cys polymorphism in the human 8-oxogunaine DNA glycosylase (hOGG1) gene had been implicated in cancer susceptibility. Studies investigating the associations between the Ser326Cys polymorphism and cancer susceptibility showed conflicting results. To derive a more precise estimation of the relationship, a meta...

journal_title：Mutagenesis

authors： Wang W,Wang M,Chen Y,Zhang Z,Wang S,Xu M,Wang B,Zhao Q,Zhang Z

更新日期：2012-05-01 00:00:00

abstract：:Inter-individual differences in susceptibility to breast cancer are partially mediated through the levels of endogenous and exogenous steroid hormones. The CYP17 gene encodes P450c17alpha, an enzyme that is involved in the metabolism of steroid hormones. Increased endogenous steroid hormone levels have been associated...

journal_title：Mutagenesis

authors： Ye Z,Parry JM

更新日期：2002-03-01 00:00:00

abstract：:The widespread production and use of nanoparticles calls for faster and more reliable methods to assess their safety. The main aim of this study was to investigate the genotoxicity of three reference TiO2 nanomaterials (NM) within the frame of the FP7-NANoREG project, with a particular focus on testing the applicabili...

journal_title：Mutagenesis

authors： Di Bucchianico S,Cappellini F,Le Bihanic F,Zhang Y,Dreij K,Karlsson HL

更新日期：2017-01-01 00:00:00

abstract：:On April 6, 1993, near the town of Tomsk (Russia) there was an accident at the Siberian Chemical Plant (SCP) which resulted in extensive contamination of an area of 250 km(2) to the north of SCP with long-lived radionuclides such as (239)Pu, (137)Cs and (90)Sr. Cytogenetic methods and electron spin resonance (ESR) spe...

journal_title：Mutagenesis

authors： Ilyinskikh NN,Ilyinskikh IN,Porovskiy VA,Natarajan AT,Suskov II,Smirenniy LN,Ilyinskikh EN

更新日期：1999-09-01 00:00:00

abstract：:Over the past several years, we have been evaluating the mutagenicity and clastogenicity of compounds capable of Michael-type reactions. These compounds, including acrylamide, several acrylate and methacrylate esters, vinyl sulfones, and phorone, have been evaluated using TK+/- -3.7.2C mouse lymphoma cells. Mutagenic ...

journal_title：Mutagenesis

authors： Dearfield KL,Harrington-Brock K,Doerr CL,Rabinowitz JR,Moore MM

更新日期：1991-11-01 00:00:00

abstract：:Wood dust is recognised as a human carcinogen, based on the strong association of wood dust exposure and the elevated risk of malignant tumours of the nasal cavity and paranasal sinuses [sino-nasal cancer (SNC)]. The study aimed to assess genetic damage in workers exposed to wood dust using biomarkers in both buccal a...

journal_title：Mutagenesis

authors： Bruschweiler ED,Hopf NB,Wild P,Huynh CK,Fenech M,Thomas P,Hor M,Charriere N,Savova-Bianchi D,Danuser B

更新日期：2014-05-01 00:00:00

abstract：:An established human hepatoma cell strain (Hep G2) was used in micronuclei (MN) and sister chromatid exchange (SCE) assays to evaluate the clastogenic potential of several indirectly-acting mutagenic carcinogens. Benzo[a]pyrene, cyclophosphamide, dimethyl nitrosamine, hexamethylphosphoramide, pyrene and safrole were s...

journal_title：Mutagenesis

authors： Natarajan AT,Darroudi F

更新日期：1991-09-01 00:00:00

abstract：:FD&C Red No.3 (erythrosine) has been used as a dye in foods, drugs and cosmetics since its approval by the US Department of Agriculture in 1907. In 1977 the Certified Color Manufacturers' Association (CCMA) initiated studies on FD&C Red No.3 including chronic toxicity and carcinogenicity studies in rats and mice. Data...

journal_title：Mutagenesis

authors： Lin GH,Brusick DJ

更新日期：1986-07-01 00:00:00

abstract：:Gaulden reported a novel and unexpected mitomycin C (MMC) effect, namely a pronounced retardation of very late prophase and loss of chromosome orientation in neuroblasts of the grasshopper Chortophaga viridifasciate. Because this effect may be due to interactions of MMC with non-DNA targets, MMC was tested for its int...

journal_title：Mutagenesis

authors： Albertini S,Friederich U,Würgler FE

更新日期：1989-01-01 00:00:00

abstract：:Exposure to ambient air particles is associated with elevated levels of DNA strand breaks (SBs) and endonuclease III, formamidopyrimidine DNA glycosylase (FPG) and oxoguanine DNA glycosylase-sensitive sites in cell cultures, animals and humans. In both animals and cell cultures, increases in SB and in oxidatively dama...

journal_title：Mutagenesis

authors： Møller P,Hemmingsen JG,Jensen DM,Danielsen PH,Karottki DG,Jantzen K,Roursgaard M,Cao Y,Kermanizadeh A,Klingberg H,Christophersen DV,Hersoug LG,Loft S

更新日期：2015-01-01 00:00:00

abstract：:We examined the clastogenic and cell-transforming potential of ultrafine particulate matter fraction PM0.056 of urban ambient aerosol using mammalian cells. PM1.0, PM0.56 and PM0.056 fractions were sampled from roadside atmosphere of an urban area using the cascade impactor MOUDI-NR-110. The potential to induce cytoto...

journal_title：Mutagenesis

authors： Verma MK,Poojan S,Sultana S,Kumar S

更新日期：2014-09-01 00:00:00

abstract：:Transgenic B6C3F1 and C57BL/6 mice containing a lambda shuttle vector that carries a lacI target and an alpha lacZ reporter gene have been constructed for use in in vivo mutagenesis assays. After chemical treatment of mice carrying the lacI target gene, genomic DNA is isolated and the shuttle vector is recovered by ex...

journal_title：Mutagenesis

authors： Mirsalis JC,Provost GS,Matthews CD,Hamner RT,Schindler JE,O'Loughlin KG,MacGregor JT,Short JM

更新日期：1993-05-01 00:00:00

abstract：:Urine samples from 26 cigarette smokers on a restricted diet were collected in the late afternoon. Urine extracts on XAD-2 resin were tested for mutagenicity in the microsuspension assay using Salmonella typhimurium strain TA98 in the presence of metabolizing and deconjugating enzymes. Levels of urinary nicotine plus ...

journal_title：Mutagenesis

authors： Granella M,Priante E,Nardini B,Bono R,Clonfero E

更新日期：1996-03-01 00:00:00

abstract：:Among the tests used to determine the mutagenic potential of chemicals, the chromosomal aberrations and micronucleus assays play an important role. These tests score either chromosomal structural aberrations at metaphase or micronuclei at interphase. One of the hallmarks of apoptosis is DNA fragmentation into 50-300 k...

journal_title：Mutagenesis

authors： Meintières S,Biola A,Pallardy M,Marzin D

更新日期：2001-05-01 00:00:00

abstract：:The induction of localized DNA damage within a discrete nuclear volume is an important tool in DNA repair studies. Both charged particle irradiation and laser microirradiation (LMI) systems allow for such a localized damage induction, but the results obtained are difficult to compare, as the delivered laser dose canno...

journal_title：Mutagenesis

authors： Splinter J,Jakob B,Lang M,Yano K,Engelhardt J,Hell SW,Chen DJ,Durante M,Taucher-Scholz G

更新日期：2010-05-01 00:00:00

abstract：:During the past four years several livestock farms (sheep, cattle and river buffalo) in the provinces of Naples and Caserta (southern Italy) have been unable to sell their milk and other dairy products due to the levels of dioxins (17 different types) present in the milk mass exceeding the value permitted [3 pg/g of f...

journal_title：Mutagenesis

authors： Perucatti A,Di Meo GP,Albarella S,Ciotola F,Incarnato D,Jambrenghi AC,Peretti V,Vonghia G,Iannuzzi L

更新日期：2006-01-01 00:00:00

abstract：:Newborns have to cope with hypoxia during delivery and a sudden increase in oxygen at birth. Oxygen will partly be released as reactive oxygen species having the potential to cause damage to DNA and proteins. In utero, increase of most (non)-enzymatic antioxidants occurs during last weeks of gestation, making preterm ...

journal_title：Mutagenesis

authors： Vande Loock K,Ciardelli R,Decordier I,Plas G,Haumont D,Kirsch-Volders M

更新日期：2012-09-01 00:00:00

abstract：:In vivo genotoxicity studies-shortly followed by carcinogenicity-are posing high demand for test-related recourses in terms of animal lives and resources. Among those, the micronucleus test in rodents is the most widely used as a follow-up to positive in vitro mutagenicity results; therefore, the development and exten...

journal_title：Mutagenesis

authors： Benigni R,Bossa C,Worth A

更新日期：2010-07-01 00:00:00

abstract：:Monitoring oxidative stress in vivo is made easier by the ability to use samples obtained non-invasively, such as urine. The analysis of DNA oxidation, by measurement of oxidized 2'-deoxyribonucleosides in urine, particularly 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), has been reported extensively in the literatur...

journal_title：Mutagenesis

authors： Evans MD,Saparbaev M,Cooke MS

更新日期：2010-09-01 00:00:00

abstract：:Barrett's oesophagus (BO) carries an increased risk of progression to oesophageal adenocarcinoma. Chromoendoscopy with methylene blue (MB) can be used to facilitate identification of BO and target areas for biopsy. If photoexcited, MB can generate reactive oxygen species and genotoxic photodegradation products leading...

journal_title：Mutagenesis

authors： Sturmey RG,Wild CP,Hardie LJ

更新日期：2009-05-01 00:00:00

abstract：:In order to discriminate between different mechanisms put forward to explain the low observed yields of reciprocal translocation configurations at diakinesis-metaphase I spermatocytes following treatment of mouse stem cell spermatogonia with chemical clastogens, the physiological states of stem cells at the moment of ...

journal_title：Mutagenesis

authors： van Buul PP,Seelen CM,Schöppink K,Goudzwaard JH

更新日期：1992-07-01 00:00:00

abstract：:The effects of deficiency in the DNA mismatch repair (MMR) protein Pms2 were investigated using the endogenous mouse Hprt and Tk genes as reporters of intragenic mutation and loss of heterozygosity (LOH). Pms2(-/-)Tk(+/-), Pms2(+/+)Tk(+/-), Pms2(+/-)Tk(+/-) and Pms2(-/-)Tk(-/-) mice were bred from Pms2(+/-)Tk(+/-) mic...

journal_title：Mutagenesis

authors： Dobrovolsky VN,McKinzie PB,Shaddock JG,Mittelstaedt RA,Heflich RH,Parsons BL

更新日期：2003-07-01 00:00:00

abstract：:The cytogenetic effects of mimosine, a naturally occurring plant amino acid known to arrest cell-cycle progression at the G1-S border in cultured cells, have been studied. It was found that mimosine inhibits the cell-cycle progression in a dose-dependent manner in primary and transformed Chinese hamster fibroblasts as...

journal_title：Mutagenesis

authors： Jha AN,Hande PM,Mullenders LH,Natarajan AT

更新日期：1995-09-01 00:00:00