Structural analysis and predictive value of the rodent in vivo micronucleus assay results.

abstract：:Hyperbaric oxygen (HBO) treatment as used therapeutically has been shown to induce DNA damage in the alkaline comet assay with leukocytes from test subjects. Using formamidopyrimidine-DNA glycosylase, a DNA repair enzyme which specifically nicks DNA at sites of 8-oxoguanines and formamidopyrimidines, we have detected ...

journal_title：Mutagenesis

authors： Speit G,Dennog C,Lampl L

更新日期：1998-01-01 00:00:00

abstract：:The nucleoside analog zidovudine (3'-azido-3'-deoxythymidine, AZT), by itself or in combination with other anti- retroviral drugs, is used perinatally to prevent mother to child transmission of human immunodeficiency virus type 1. AZT is mutagenic in vitro and mutagenic and carcinogenic when administered to neonatal m...

journal_title：Mutagenesis

authors： Von Tungeln LS,Dobrovolsky VN,Bishop ME,Shaddock JG,Heflich RH,Beland FA

更新日期：2004-07-01 00:00:00

abstract：:The objective of the present study was to develop a cellular phenotype assay for nucleotide excision repair (NER), using benzo[a]pyrene diol epoxide (BPDE) as model mutagen. Since in vitro exposure to BPDE may lead to DNA strand breaks resulting from both direct interaction with DNA and incisions introduced by the rep...

journal_title：Mutagenesis

authors： Vande Loock K,Decordier I,Ciardelli R,Haumont D,Kirsch-Volders M

更新日期：2010-01-01 00:00:00

abstract：:We have developed a two dose/single-sample protocol for the in vivo/in vitro rat hepatocyte unscheduled DNA synthesis (UDS) assay. In order to enhance the effectiveness of grain detection by image analysis we found minor technical modifications to be beneficial. These included the use of 3% acetic acid in ethanol (or ...

journal_title：Mutagenesis

authors： Amphlett NW,Mitchell ID,Rees RW,Haynes GA

更新日期：1996-01-01 00:00:00

abstract：:Gaulden reported a novel and unexpected mitomycin C (MMC) effect, namely a pronounced retardation of very late prophase and loss of chromosome orientation in neuroblasts of the grasshopper Chortophaga viridifasciate. Because this effect may be due to interactions of MMC with non-DNA targets, MMC was tested for its int...

journal_title：Mutagenesis

authors： Albertini S,Friederich U,Würgler FE

更新日期：1989-01-01 00:00:00

abstract：:Chromatographic profiles of DNA adducts formed following treatment of Chinese hamster ovary (CHO) cells or C3H10T1/2 mouse embryo fibroblast cells with benzo[a]pyrene (BP), in the presence or absence of rat liver S9 fraction, have been obtained by both direct labelling ([3H]BP) and 32P-post-labelling methods. The prin...

journal_title：Mutagenesis

authors： Watson WP,Smith RJ,Ryan D,Meyer AL

更新日期：1990-07-01 00:00:00

abstract：:Adducts of 1,2-epoxy-3-butene (EB) with haemoglobin were monitored in 17 workers from the 1,3-butadiene (BD) production unit of a petrochemical plant and in nine referents employed at the same factory but not exposed to BD. The air concentrations of BD were determined using stationary and personal monitoring. The ambi...

journal_title：Mutagenesis

authors： Osterman-Golkar S,Peltonen K,Anttinen-Klemetti T,Landin HH,Zorcec V,Sorsa M

更新日期：1996-03-01 00:00:00

abstract：:Newborns have to cope with hypoxia during delivery and a sudden increase in oxygen at birth. Oxygen will partly be released as reactive oxygen species having the potential to cause damage to DNA and proteins. In utero, increase of most (non)-enzymatic antioxidants occurs during last weeks of gestation, making preterm ...

journal_title：Mutagenesis

authors： Vande Loock K,Ciardelli R,Decordier I,Plas G,Haumont D,Kirsch-Volders M

更新日期：2012-09-01 00:00:00

abstract：:A transgenic mouse strain with a high copy number of rescuable lacZ sequences was evaluated for its effectiveness in detecting lacZ- mutations in selected tissues. Procarbazine, cyclophosphamide, ethylnitrosourea, 7,12-dimethylbenz[a]anthracene (DMBA), acrylamide and chlorambucil were tested following either single or...

journal_title：Mutagenesis

authors： Hoorn AJ,Custer LL,Myhr BC,Brusick D,Gossen J,Vijg J

更新日期：1993-01-01 00:00:00

abstract：:The mechanism responsible for the modification of mutagenicity by chlorophyllin has been investigated using mutagenic compounds with different mechanisms of action, including the monofunctional alkylating agents, N-methyl-N'-nitrosourea (MNU) and ethylmethanesulphonate (EMS); nitrosamines related to tobacco products, ...

journal_title：Mutagenesis

authors： Romert L,Curvall M,Jenssen D

更新日期：1992-09-01 00:00:00

abstract：:The repairability during G(1) of DNA lesions eliciting sister chromatid exchanges (SCE) induced by methylmethanesulfonate (MMS) and ethylmethanesulfonate (EMS) in BrdU-substituted and unsubstituted DNA strands was determined in murine salivary gland cells in vivo. The SCE frequency was determined after exposure to MMS...

journal_title：Mutagenesis

authors： González-Beltrán F,Morales-Ramírez P

更新日期：2003-01-01 00:00:00

abstract：:The restriction site mutation method described by Parry et al. (1990) for isolating mutant sequences directly from DNA is discussed and an additional enrichment step proposed using magnetic beads. ...

journal_title：Mutagenesis

authors： Bridges BA,Carr AM,Lehmann AR

更新日期：1990-11-01 00:00:00

abstract：:Alcohol drinking-derived acetaldehyde is believed to cross-link DNA and induce sister chromatid exchanges in peripheral blood lymphocytes. However, little population data are available to illustrate effects of alcohol-derived acetaldehyde on DNA migration as assayed by the comet assay in peripheral lymphocytes. In the...

journal_title：Mutagenesis

authors： Lu Y,Morimoto K

更新日期：2009-07-01 00:00:00

abstract：:This is an investigation of the long-term effects of a shift from a mixed diet to a lacto-vegetarian diet and of a return to a mixed diet on the mutagenic activity in urine and faeces. The participants were 20 normal weight, non-smoking subjects. Dietary surveys and urinary and faecal samples were collected before and...

journal_title：Mutagenesis

authors： Johansson G,Holmén A,Persson L,Högstedt B,Wassén C,Ottova L,Gustafsson JA

更新日期：1998-03-01 00:00:00

abstract：:Nitropyrenes are a class of polycyclic hydrocarbons that have been shown to be potent point mutagens in the Salmonella/mammalian microsome assay. However, relatively few data have been published on their ability to induce chromosome aberrations. In this paper we report the cytogenetic activity of 1-nitropyrene, 1-nitr...

journal_title：Mutagenesis

authors： Lafi A,Parry JM

更新日期：1987-01-01 00:00:00

abstract：:Male infertility is a relevant social and medical problem. Male infertility is mostly caused by genetic disorders. The purpose of the study was to analyze the correlation of chromosome aberrations, as well as DNA fragmentation and various manifestations of spermatogenesis disorder. Sperm samples of 58 males with infer...

journal_title：Mutagenesis

authors： Alchinbayev MK,Aralbayeva AN,Tuleyeva LN,Duysenbayeva SM,Makazhanov MA

更新日期：2016-09-01 00:00:00

abstract：:Well-established in vitro methods for testing the genotoxic potency of chemicals--such as the Ames/Salmonella test, the mouse lymphoma assay, the micronucleus test and the chromosomal aberration test--show a high false-positive rate for predicting in vivo genotoxicity and carcinogenicity. Thus, there is a need for mor...

journal_title：Mutagenesis

authors： Mathijs K,Brauers KJ,Jennen DG,Lizarraga D,Kleinjans JC,van Delft JH

更新日期：2010-11-01 00:00:00

abstract：:The Mre11 complex (Mre11-Rad50-Nbs1) is involved in a diverse array of DNA metabolic processes including the response to DNA double-strand breaks (DSBs). The structure of Rad50 plays a key role in the DNA-binding and end-bridging activity of the complex. An interesting feature within the central portion of the Rad50 p...

journal_title：Mutagenesis

authors： Cahill D,Carney JP

更新日期：2007-07-01 00:00:00

abstract：:Non-homologous end joining (NHEJ), the major pathway of double-strand break (DSB) repair in mammalian cells, comprises two subpathways: one that requires the three core factors Ku70/80, DNA-PKcs and XRCC4/LigIV (DNA-PK-dependent NHEJ) and the other that is independent of these factors. Using a cell-free NHEJ assay, we...

journal_title：Mutagenesis

authors： Kuhfittig-Kulle S,Feldmann E,Odersky A,Kuliczkowska A,Goedecke W,Eggert A,Pfeiffer P

更新日期：2007-05-01 00:00:00

abstract：:4-Nitroquinoline 1-oxide (4NQO) is used as a positive control in various genotoxicity assays because of its known mutagenic and carcinogenic properties. The chemical is converted into 4-hydroxyaminoquinoline 1-oxide and gives rise to three main DNA adducts, N-(deoxyguanosin-8-yl)-4AQO, 3-(desoxyguanosin-N (2)-yl)-4AQO...

journal_title：Mutagenesis

authors： Brüsehafer K,Manshian BB,Doherty AT,Zaïr ZM,Johnson GE,Doak SH,Jenkins GJ

更新日期：2016-03-01 00:00:00

abstract：:The over-production of reactive oxygen species (ROS) can cause oxidative damage to a large number of molecules, including DNA, and has been associated with the pathogenesis of several disorders, such as diabetes mellitus (DM), dyslipidemia and periodontitis (PD). We hypothesise that the presence of these diseases coul...

journal_title：Mutagenesis

authors： Corbi SC,Bastos AS,Orrico SR,Secolin R,Dos Santos RA,Takahashi CS,Scarel-Caminaga RM

更新日期：2014-11-01 00:00:00

abstract：:During the past four years several livestock farms (sheep, cattle and river buffalo) in the provinces of Naples and Caserta (southern Italy) have been unable to sell their milk and other dairy products due to the levels of dioxins (17 different types) present in the milk mass exceeding the value permitted [3 pg/g of f...

journal_title：Mutagenesis

authors： Perucatti A,Di Meo GP,Albarella S,Ciotola F,Incarnato D,Jambrenghi AC,Peretti V,Vonghia G,Iannuzzi L

更新日期：2006-01-01 00:00:00

abstract：:The adduct that would arise from cis opening of (+)-(1S,2R,3R, 4S)-3,4-dihydroxy-1,2-epoxy-benzo[c]phenan-threne (benzo[c]phenanthrene diol epoxide-2, where the benzylic hydroxyl group and the epoxide oxygen are trans) by the exocyclic N6-amino group of deoxyadenosine was incorporated at the marked site into four olig...

journal_title：Mutagenesis

authors： Pontén I,Waters LS,Sayer JM,Pilcher AS,Dipple A,Jerina DM

更新日期：2001-01-01 00:00:00

abstract：:Dibenz[a,h]anthracene (DB[a,h]A) is a polycyclic aromatic hydrocarbon that is a by-product of combustion and a potent carcinogen. Few studies have investigated the effects of DB[a,h]A on mRNA and microRNA expression to dissect the mechanisms involved in carcinogenesis. In this study, mature male mice (Muta(™)Mouse) we...

journal_title：Mutagenesis

authors： Malik AI,Rowan-Carroll A,Williams A,Lemieux CL,Long AS,Arlt VM,Phillips DH,White PA,Yauk CL

更新日期：2013-09-01 00:00:00

abstract：:The spectrum of mutations induced by cobalt(II) chloride (CoCl2) was examined using plasmid pUB3 DNA, which was propagated after transfection into Escherichia coli SY1032/pKY241 host cells. The vector plasmid carried an E.coli supF suppressor tRNA gene as a target for mutations. After CoCl2 treatment, 64 independent n...

journal_title：Mutagenesis

authors： Ogawa HI,Ohyama Y,Ohsumi Y,Kakimoto K,Kato Y,Shirai Y,Nunoshiba T,Yamamoto K

更新日期：1999-03-01 00:00:00

abstract：:It has been hypothesised that positive associations between age and levels of oxidative stress-generated damage to DNA may be related to an age-dependent decline in DNA repair activity. The objective of this study was to investigate the association between age and repair activity of oxidatively damaged DNA in peripher...

journal_title：Mutagenesis

authors： Løhr M,Jensen A,Eriksen L,Grønbæk M,Loft S,Møller P

更新日期：2015-09-01 00:00:00

abstract：:I suggest that there are several good reasons for retaining mammalian cell mutation assays in a basic test package for genotoxicity and that the possible existence of a non-clastogenic mammalian mutagen is not one of them. I put forward a number of propositions, not necessarily original, concerning in vitro assays, in...

journal_title：Mutagenesis

authors： Green MH

更新日期：1988-09-01 00:00:00

abstract：:The complementation group (CG) assignment is presented for 74 moderately sensitive (approximately 2-4x sensitivity of parental line based on ratio of D10s) UV-sensitive mutants of Chinese hamster ovary (CHO) cells from the Facility for Automated Experiments in Cell Biology (FAECB) collection. The distribution of mutan...

journal_title：Mutagenesis

authors： Busch D,Greiner C,Rosenfeld KL,Ford R,de Wit J,Hoeijmakers JH,Thompson LH

更新日期：1994-07-01 00:00:00

abstract：:In response to DNA damage, cell cycle arrest, apoptosis, and DNA repair are mediated by a TP53 pathway that induces p21(WAF1/Cip1). The chemotherapeutic drug cis-diamminedichloroplatinum-II (cisplatin) damages cellular DNA by forming cis-diammineplatinum-N(7)-d[GpG] and cis-diammine-platinum-N(7)-d[ApG] adducts. To in...

journal_title：Mutagenesis

authors： van Gijssel HE,Leil TA,Weinberg WC,Divi RL,Olivero OA,Poirier MC

更新日期：2007-01-01 00:00:00

abstract：:Aneuploidy of mouse chromosome 8 induced by a Chinese medicinal herb, Tripterygium hypoglaucum (level) Hutch (THH) was investigated by fluorescence in situ hybridization (FISH) in vivo. Male mice were treated with THH (single i.p. injection) at doses of 120, 240 and 480 mg/kg. Colchicine (COL, 1.5 mg/kg i.p.) was used...

journal_title：Mutagenesis

authors： Xu W,Ziqing L,Yinrun D,Xiaoyan W,Jinglun X

更新日期：2004-09-01 00:00:00