Abstract:
:The repairability during G(1) of DNA lesions eliciting sister chromatid exchanges (SCE) induced by methylmethanesulfonate (MMS) and ethylmethanesulfonate (EMS) in BrdU-substituted and unsubstituted DNA strands was determined in murine salivary gland cells in vivo. The SCE frequency was determined after exposure to MMS or EMS during early and late G(1) in the first or second cell division. The inducibility and repairability of SCE-eliciting lesions during G(1) in BrdU-substituted and unsubstituted strands were estimated considering that in the first division induction occurs on the unsubstituted strand and during the second division in one unsubstituted and one BrdU- substituted DNA strand. The results indicate that DNA lesions induced by MMS are 50% repaired in both the BrdU-substituted and unsubstituted strands and those induced by EMS are 60% repaired in the unsubstituted strand but only 20% in the BrdU-substituted strand. The increase in sensitivity of the BrdU-substituted strand to SCE induction with respect to the unsubstituted strand was 155 and 45% for MMS and EMS, respectively. These results imply that SCE-inducing lesions produced by MMS and EMS are only partially repaired and that BrdU incorporation could sensitize DNA not only to the induction of lesions eliciting SCE, but also to the induction of non-repairable lesions.
journal_name
Mutagenesisjournal_title
Mutagenesisauthors
González-Beltrán F,Morales-Ramírez Pdoi
10.1093/mutage/18.1.13subject
Has Abstractpub_date
2003-01-01 00:00:00pages
13-7issue
1eissn
0267-8357issn
1464-3804journal_volume
18pub_type
杂志文章相关文献
MUTAGENESIS文献大全abstract::In the past decade, a number of case-control studies have been carried out to investigate the relationship between the HHEX polymorphism and type 2 diabetes (T2D). However, the results have been inconclusive. To investigate this inconsistency, we performed a meta-analysis of all available studies dealing with the rela...
journal_title:Mutagenesis
pub_type: 杂志文章,meta分析
doi:10.1093/mutage/geq095
更新日期:2011-03-01 00:00:00
abstract::All of the published mutagenicity data generated using the Muta Mouse (lacZ) and the Big Blue (lacI) transgenic mouse mutation assays have been re-presented in a standard format. To date, 26 chemicals have been evaluated in one or other of the assays, and eight tissues have been sampled. The present analysis should ex...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/9.4.367
更新日期:1994-07-01 00:00:00
abstract::The aneuploidy inducing capacity of 2-chlorobenzylidene malonitrile (CS), a chemical used as a riot control agent, and its hydrolysis products o-chlorobenzaldehyde and malonitrile was studied at various exposure conditions in V79 Chinese hamster cells. Chromosomes were counted in metaphase preparations with preserved ...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/6.4.303
更新日期:1991-07-01 00:00:00
abstract::The ability of topoisomerase II inhibitor, teniposide, to induce aneuploidy and meiotic delay in somatic and germinal cells of male mice was investigated by fluorescence in situ hybridisation (FISH) assay using labelled DNA probes and 5-bromo-2'-deoxyuridine (BrdU) incorporation assay, respectively. Colchicine and mit...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/ger051
更新日期:2012-01-01 00:00:00
abstract::The induction of localized DNA damage within a discrete nuclear volume is an important tool in DNA repair studies. Both charged particle irradiation and laser microirradiation (LMI) systems allow for such a localized damage induction, but the results obtained are difficult to compare, as the delivered laser dose canno...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/geq005
更新日期:2010-05-01 00:00:00
abstract::Famotidine, a histamine H2-receptor antagonist, was devoid of mutagenic activity in seven his- Salmonella typhimurium strains (TA1535, TA1537, TA1538, TA97, TA98, TA100 and TA102) and was equitoxic in repair-proficient (WP2) and repair-deficient (WP2uvrA, WP67, CM561, CM571, WP100 and CM871) Escherichia coli strains, ...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/1.2.125
更新日期:1986-03-01 00:00:00
abstract::The toxicological relevance of the micronucleus (MN) test is well defined: it is a multi-target genotoxic endpoint, assessing not only clastogenic and aneugenic events but also some epigenetic effects, which is simple to score, accurate, applicable in different cell types. In addition, it is predictive for cancer, ame...
journal_title:Mutagenesis
pub_type: 杂志文章,评审
doi:10.1093/mutage/geq068
更新日期:2011-01-01 00:00:00
abstract::Dibenz[a,h]anthracene (DB[a,h]A) is a polycyclic aromatic hydrocarbon that is a by-product of combustion and a potent carcinogen. Few studies have investigated the effects of DB[a,h]A on mRNA and microRNA expression to dissect the mechanisms involved in carcinogenesis. In this study, mature male mice (Muta(™)Mouse) we...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/get031
更新日期:2013-09-01 00:00:00
abstract::This study investigated the effect of glucose and fructose, and advanced glycation end-products (AGEs) on genome damage in WIL2-NS cells, measured using the cytokinesis-block micronucleus cytome (CBMN-Cyt) assay. The effect of AGEs was investigated using the bovine serum albumin (AGE-BSA) model system induced either w...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/geaa002
更新日期:2020-03-27 00:00:00
abstract::Isothiocyanates are plant-derived compounds that may be beneficial in the prevention of certain chronic diseases. Yet, by stimulating the production of reactive oxygen species (ROS), isothiocyanates can be genotoxic. Whether antioxidants influence isothiocyanate-induced genotoxicity is unclear, but this situation was ...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gev003
更新日期:2015-05-01 00:00:00
abstract::The circulating human transcriptome, which includes both coding and non-coding RNA (ncRNA) molecules, represents a rich source of potential biomarkers for colorectal cancer (CRC) that has only recently been explored. In particular, the release of RNA-containing extracellular vesicles (EVs), in a multitude of different...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gez038
更新日期:2020-07-11 00:00:00
abstract::A relatively large, reproducible inter-individual variation was found in the ability of 17 human liver S9 samples to mediate the mutagenicity of 2-acetylaminofluorene (2AAF) in Salmonella typhimurium strain TA98. In an animal model, variation in metabolic activation of 2AAF did not appear to relate to the phenotype of...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/3.4.323
更新日期:1988-07-01 00:00:00
abstract::Potential toxicological interactions of 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and/or dibutyl phthalate (DBP) with ozone were investigated. Male and female B6C3F1 mice were exposed to ozone (0.5 p.p.m.), NNK (1.0 mg/kg), DBP (5000 p.p.m.) and different combinations of these toxicants 6 h/day for 16,...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/17.4.331
更新日期:2002-07-01 00:00:00
abstract::Prostate cancer is a major health burden, being the second most commonly diagnosed malignancy in men worldwide. Overtreatment represents a major problem in prostate cancer therapy, leading to significant long-term quality-of-life effects for patients and a broad socio-ecological burden. Biomarkers that could facilitat...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/geaa010
更新日期:2020-07-11 00:00:00
abstract::Carbaryl causes depolymerization of spindle microtubules and apparent uncoupling of karyokinesis and cytokinesis in mitotic V79 cells. The metabolite 1-naphthol has virtually identical effects at equimolar concentrations. The closely related 2-naphthol causes similar configurations, but at a much lower frequency than ...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/13.4.345
更新日期:1998-07-01 00:00:00
abstract::Genetic damage in workers occupationally exposed to an antineoplastic drug was studied using the micronucleus (MN) test, the comet assay, the hprt gene mutation assay and the TCR gene mutation assay. The subjects were divided into two groups: (i) 21 workers from a plant producing methotrexate (MTX); (ii) 21 controls w...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gei048
更新日期:2005-09-01 00:00:00
abstract::The effect of cerium oxide nanoparticle (CeNP) in plants has elicited substantial controversy. While some investigators have reported that CeNP possesses antioxidant properties, others observed CeNP to induce reactive oxygen species (ROS). In spite of considerable research carried out on the effects of CeNP in metazoa...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gey004
更新日期:2018-04-13 00:00:00
abstract::In this study, we determined the induction and time-dependent accumulation of micronuclei in the peripheral blood of transgenic C57BL/6 p53+/- mice (p53+/- mice), FVB/N Tg.AC v-Ha-ras mice (Tg.AC mice) and their isogenic parental strains, FVB/N and C57BL/6 following inhalation exposure to benzene. Our objective was to...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/16.2.163
更新日期:2001-03-01 00:00:00
abstract::Photomutagenicity assays are required for regulatory submissions of some chemicals. As yet there are no well-validated protocols available for these assays. Critical factors which may contribute to the ability of a bacterial assay to detect photomutagens (e.g. dose of UV and test chemical, exposure conditions, light s...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/9.5.459
更新日期:1994-09-01 00:00:00
abstract::Coal miners are exposed to coal dust, containing mineral particles, inorganic compounds and polycyclic aromatic hydrocarbons, and to ionizing radiation. These factors can induce oxidative stress and promote inflammation that leads to DNA damage. The aim of this investigation is to analyse the degree of DNA damage in m...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gew038
更新日期:2016-11-01 00:00:00
abstract::This is an investigation of the long-term effects of a shift from a mixed diet to a lacto-vegetarian diet and of a return to a mixed diet on the mutagenic activity in urine and faeces. The participants were 20 normal weight, non-smoking subjects. Dietary surveys and urinary and faecal samples were collected before and...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/13.2.167
更新日期:1998-03-01 00:00:00
abstract::Many chronic inflammatory conditions are associated with an increased risk of cancer development. At the site of inflammation, cellular DNA is damaged by hypochlorous acid (HOCl), a potent oxidant generated by myeloperoxidase. 8-Chloro-2'-deoxyguanosine (8-Cl-dG) is a major DNA adduct formed by HOCl and has been detec...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/ges056
更新日期:2013-01-01 00:00:00
abstract::Coleus aromaticus (Benth, Family: Laminaceae), Indian Oregano native to India and Mediterranean, is well known for its medicinal properties. A preliminary study was undertaken to elucidate in vitro free radical scavenging potential and inhibition of lipid peroxidation by C.aromaticus hydroalcoholic extract (CAE). Anti...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gel023
更新日期:2006-07-01 00:00:00
abstract::This paper presents the results obtained within the framework of an EU research project aimed at investigating the relationship between occupational exposure to pesticides and the induction of cytogenetic damage. Populations from Greece, Spain, Poland and Hungary, all of them characterised by intensive agricultural ac...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/18.3.249
更新日期:2003-05-01 00:00:00
abstract::It has been hypothesised that positive associations between age and levels of oxidative stress-generated damage to DNA may be related to an age-dependent decline in DNA repair activity. The objective of this study was to investigate the association between age and repair activity of oxidatively damaged DNA in peripher...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gev031
更新日期:2015-09-01 00:00:00
abstract::A modified method for the 32P-postlabeling assay that permits standardized quantitation for specific polycyclic aromatic hydrocarbon (PAH)-DNA adducts is described. This method has been designed to test the components of the 32P-postlabeling assay for use in the chemically specific detection of individual adducts with...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/8.2.121
更新日期:1993-03-01 00:00:00
abstract::Recent problems with the supply of Difco bacto agar have forced some laboratories to evaluate alternative agars for use in the Salmonella/microsome assay. This led to the independent observation in two laboratories (Boots and Glaxo) that Salmonella typhimurium TA97a is sensitive to certain types of agar that may be us...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/7.1.13
更新日期:1992-01-01 00:00:00
abstract::As part of the hazard and risk assessment of chemicals in man, it is important to assess the ability of a chemical to induce mutations in vivo. Because of the commonalities in the molecular initiating event, mutagenicity in vitro can correlate well to the in vivo endpoint for certain compound classes; however, the dif...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gey030
更新日期:2019-03-06 00:00:00
abstract::Activation of the human bladder carcinogen 4-aminobiphenyl (ABP) and its N-hydroxy derivatives was investigated using lysates and subcellular enzyme preparations from cultured human uroepithelial cells (HUC). Mutagenic activation was determined using Salmonella typhimurium strains TA98; TA98/1,8-DNP6, a derivative def...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/8.2.113
更新日期:1993-03-01 00:00:00
abstract::The presence of centromeric DNA was studied in micronuclei isolated from the blood of male ddY mice after five weekly intraperitoneal injections of mitomycin C (MMC), 1-beta-D-arabinofuranosylcytosine (Ara-C), colchicine (COL) or vinblastine sulfate (VBL). In agreement with our earlier findings, about half of the micr...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/10.6.513
更新日期:1995-11-01 00:00:00