Extrapolation of in vitro structural alerts for mutagenicity to the in vivo endpoint.

abstract：:Basic extracts isolated from lamb meat treated at various temperatures were tested for mutagenicity in Salmonella typhimurium strain TA98 in the presence of S9 mix. Samples of ground lamb patties were cooked for 10 min per side at 100, 150, 200, 250 and 300 degrees C. Low cooking temperatures resulted in products with...

journal_title：Mutagenesis

authors： Sflomos C,Papadopoulou R,Athanasiou K

更新日期：1989-05-01 00:00:00

abstract：:The aim of the present work was to investigate the processes involved in the origin of trisomic karyotypes, i.e. co-migration of sister chromatids (mitotic non-disjunction, MND) and recovery of micronuclei (MN) originating from lagging chromosomes/chromatids at anaphase (mitotic indirect non-disjunction, MIND), and to...

journal_title：Mutagenesis

authors： Minissi S,Degrassi F,Tanzarella C,Gustavino B

更新日期：1999-11-01 00:00:00

abstract：:We report on a new method for the study of radiation-induced chromatid breaks in stimulated human peripheral blood T lymphocytes, involving a combination of a 1-h colcemid block and a short (15 min) calyculin A treatment. We find that this procedure eliminates the problem of centromere splitting when calyculin A is us...

journal_title：Mutagenesis

authors： Shovman O,Riches AC,Adamson D,Bryant PE

更新日期：2008-07-01 00:00:00

abstract：:The effects of deficiency in the DNA mismatch repair (MMR) protein Pms2 were investigated using the endogenous mouse Hprt and Tk genes as reporters of intragenic mutation and loss of heterozygosity (LOH). Pms2(-/-)Tk(+/-), Pms2(+/+)Tk(+/-), Pms2(+/-)Tk(+/-) and Pms2(-/-)Tk(-/-) mice were bred from Pms2(+/-)Tk(+/-) mic...

journal_title：Mutagenesis

authors： Dobrovolsky VN,McKinzie PB,Shaddock JG,Mittelstaedt RA,Heflich RH,Parsons BL

更新日期：2003-07-01 00:00:00

abstract：:We have developed a two dose/single-sample protocol for the in vivo/in vitro rat hepatocyte unscheduled DNA synthesis (UDS) assay. In order to enhance the effectiveness of grain detection by image analysis we found minor technical modifications to be beneficial. These included the use of 3% acetic acid in ethanol (or ...

journal_title：Mutagenesis

authors： Amphlett NW,Mitchell ID,Rees RW,Haynes GA

更新日期：1996-01-01 00:00:00

abstract：:Sister chromatid exchanges (SCE) and chromosome aberrations (CA) were studied in the lymphocytes of 70 male agricultural workers occupationally exposed to several pesticides and 69 matched controls, without indication of exposure to pesticides, from 'El Maresme' (Barcelona, Spain), Comparison between both groups revea...

journal_title：Mutagenesis

authors： Carbonell E,Xamena N,Creus A,Marcos R

更新日期：1993-11-01 00:00:00

abstract：:There are several reports demonstrating that aneugens may preferentially affect segregation of particular chromosomes in somatic cells. Much less is known on specific susceptibility of individual chromosomes to non-disjunction in mammalian meiosis in response to chemical exposures. To explore possible chromosome-speci...

journal_title：Mutagenesis

authors： Sun F,Yin H,Eichenlaub-Ritter U

更新日期：2001-09-01 00:00:00

abstract：:Adducts of 1,2-epoxy-3-butene (EB) with haemoglobin were monitored in 17 workers from the 1,3-butadiene (BD) production unit of a petrochemical plant and in nine referents employed at the same factory but not exposed to BD. The air concentrations of BD were determined using stationary and personal monitoring. The ambi...

journal_title：Mutagenesis

authors： Osterman-Golkar S,Peltonen K,Anttinen-Klemetti T,Landin HH,Zorcec V,Sorsa M

更新日期：1996-03-01 00:00:00

abstract：:Genotoxic stress causes a reduced stability of the plant genome and has a detrimental effect on plant growth and productivity. Double-strand breaks (DSBs) are the most harmful of all DNA lesions because they cause the loss of genetic information on both strands of the DNA helix. In the presented study the coding and g...

journal_title：Mutagenesis

authors： Stolarek M,Gruszka D,Braszewska-Zalewska A,Maluszynski M

更新日期：2015-11-01 00:00:00

abstract：:There is a correlation between intestinal cancer and diets high in meat, so fried beef, chicken, lamb, pork and fish were tested for their ability to induce mutations in the small intestine of mice. The mice were bred to be heterozygous at the Dlb-1 locus so that loss of the dominant Dlb-1 b allele by mutation could b...

journal_title：Mutagenesis

authors： Heddle JA,Knize MG,Dawod D,Zhang XB

更新日期：2001-03-01 00:00:00

abstract：:The major risk factor for skin cancer is exposure to UV radiation from sunlight, but other environmental exposures may also play a role in combination with UV. We have studied the effects of combined exposure of primary human skin cells in vitro to UVA, UVB or UVC with benzo[a] pyrene (BaP), an environmental carcinoge...

journal_title：Mutagenesis

authors： Crallan RA,Ingham E,Routledge MN

更新日期：2005-07-01 00:00:00

abstract：:Neurocysticercosis (NCC) has been associated with a high frequency of DNA damage in human circulating lymphocytes and more recently with the development of hematological malignancies. Chronic inflammation, a common feature of helminthic infections, has been proposed to play a key role in carcinogenesis induced by para...

journal_title：Mutagenesis

authors： Herrera LA,Rodríguez U,Gebhart E,Ostrosky-Wegman P

更新日期：2001-11-01 00:00:00

abstract：:As part of the third UKEMS collaborative trial, ICI Central Toxicology Laboratory (CTL) tested the three study chemicals ethylmethanesulphonate (EMS), benzo[a]pyrene (B[a]P) and benzidine (BZD) in a microwell adaptation of the L5178Y gene mutation assay using the thymidine kinase (tk) locus. Cofactors for metabolic ac...

journal_title：Mutagenesis

authors： Clay P,Cross MF

更新日期：1990-01-01 00:00:00

abstract：:Due to the very limited transduction capacity of hitherto available vectors, the success of gene therapy of tumours by means of suicide genes has so far essentially depended on the transfer of toxic drug metabolites from transduced (metabolizing) cells to adjacent non-transduced cells via gap junctions (bystander effe...

journal_title：Mutagenesis

authors： Thust R,Tomicic MT,Gräbner R,Friedrichs C,Wutzler P,Kaina B

更新日期：2004-01-01 00:00:00

abstract：:Three short-term assays (the SOS chromotest, the Ames-fluctuation test and the newt micronucleus test) were carried out to evaluate the genotoxicity of four trihalomethanes (chloroform, bromodichloromethane, chlorodibromomethane and bromoform). With the SOS chromotest, all the chemicals studied except chloroform were ...

journal_title：Mutagenesis

authors： Le Curieux F,Gauthier L,Erb F,Marzin D

更新日期：1995-07-01 00:00:00

abstract：:A modified protocol is described for the in vitro analysis of micronuclei in whole blood or separated lymphocyte cultures. The induction of binucleate cells by various concentrations of cytochalasin B (3, 4.5 or 6 micrograms/ml) was examined at two harvest times (68 or 72 h). An optimal yield was obtained by adding cy...

journal_title：Mutagenesis

authors： Ellard S,Parry EM

更新日期：1993-07-01 00:00:00

abstract：:This study investigated the effect of glucose and fructose, and advanced glycation end-products (AGEs) on genome damage in WIL2-NS cells, measured using the cytokinesis-block micronucleus cytome (CBMN-Cyt) assay. The effect of AGEs was investigated using the bovine serum albumin (AGE-BSA) model system induced either w...

journal_title：Mutagenesis

authors： Deo P,McCullough CL,Almond T,Jaunay EL,Donnellan L,Dhillon VS,Fenech M

更新日期：2020-03-27 00:00:00

abstract：:Monitoring oxidative stress in vivo is made easier by the ability to use samples obtained non-invasively, such as urine. The analysis of DNA oxidation, by measurement of oxidized 2'-deoxyribonucleosides in urine, particularly 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), has been reported extensively in the literatur...

journal_title：Mutagenesis

authors： Evans MD,Saparbaev M,Cooke MS

更新日期：2010-09-01 00:00:00

abstract：:Folic acid (FA), the most oxidized and stable form of folate, is commonly used as a dietary supplement and in culture media. FA must be reduced and methylated to become the metabolically active form found in blood and utilized by tissues, i.e. 5-methyltetrahydrofolate (5-MeTHF). 5-MeTHF is the methyl group donor requi...

journal_title：Mutagenesis

authors： Wang X,Fenech M

更新日期：2003-01-01 00:00:00

abstract：:Comparative investigations of sister-chromatid exchange (SCE) and micronucleus induction in the bone marrow of rats, mice and Chinese hamsters with the cytostatic alkylating mutagen cyclophosphamide (CP) revealed remarkable species differences in their mutagenic responses. With both test systems the sensitivities of t...

journal_title：Mutagenesis

authors： Madle E,Korte A,Beek B

更新日期：1986-11-01 00:00:00

abstract：:Coleus aromaticus (Benth, Family: Laminaceae), Indian Oregano native to India and Mediterranean, is well known for its medicinal properties. A preliminary study was undertaken to elucidate in vitro free radical scavenging potential and inhibition of lipid peroxidation by C.aromaticus hydroalcoholic extract (CAE). Anti...

journal_title：Mutagenesis

authors： Rao BS,Shanbhoge R,Upadhya D,Jagetia GC,Adiga SK,Kumar P,Guruprasad K,Gayathri P

更新日期：2006-07-01 00:00:00

abstract：:A simplified method, using recombinant interleukin-2, foetal bovine serum and freeze-killed feeder cells, has been developed for the mass culture of T-lymphocytes derived from human peripheral blood. In this protocol, bulk cultures can be cryopreserved approximately 8 days after initiation, and subsequent mass culture...

journal_title：Mutagenesis

authors： O'Donovan MR,Freemantle MR,Hull G,Bell DA,Arlett CF,Cole J

更新日期：1995-05-01 00:00:00

abstract：:Hyperbaric oxygen (HBO) treatment as used therapeutically has been shown to induce DNA damage in the alkaline comet assay with leukocytes from test subjects. Using formamidopyrimidine-DNA glycosylase, a DNA repair enzyme which specifically nicks DNA at sites of 8-oxoguanines and formamidopyrimidines, we have detected ...

journal_title：Mutagenesis

authors： Speit G,Dennog C,Lampl L

更新日期：1998-01-01 00:00:00

abstract：:Chemical carcinogenesis can be induced by genotoxic (GTX) or non-genotoxic (NGTX) carcinogens. GTX carcinogens have a well-described mode of action. However, the complex mechanisms by which NGTX carcinogens act are less clear and may result in conflicting results between species [e.g. Wy-14,643 (Wy)]. We hypothesise t...

journal_title：Mutagenesis

authors： Rieswijk L,Brauers KJ,Coonen ML,van Breda SG,Jennen DG,Kleinjans JC

更新日期：2015-11-01 00:00:00

abstract：:Hepatic microsomes from rats pretreated with PCB were found to metabolize the food mutagen 2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine (PhIP) to two major metabolites, one of which was identified as the N-hydroxy derivative, 2-hydroxy-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine (N-OH-PhIP). This identification w...

journal_title：Mutagenesis

authors： Frandsen H,Rasmussen ES,Nielsen PA,Farmer P,Dragsted L,Larsen JC

更新日期：1991-01-01 00:00:00

abstract：:Mutagens in the semivolatile phase of airborne particulate matter, diesel and gasoline engine emissions were investigated using chemical and biological assays. The previously unknown mutagens, 1-(3-)nitro-6-azabenzo[a]pyrenes (1-N-6-ABP and 3-N-6-ABP) and 1-(3-)nitro-6-azabenzo[a]pyrene-N-oxides (1-N-6-ABPO and 3-N-6-...

journal_title：Mutagenesis

authors： Sera N,Fukuhara K,Miyata N,Tokiwa H

更新日期：1994-01-01 00:00:00

abstract：:Aneuploidy has important consequences for human health. There is a requirement to detect aneugens that may not be fully satisfied by the current test batter. New cytogenetic techniques look promising. ...

journal_title：Mutagenesis

authors：

更新日期：1998-07-01 00:00:00

abstract：:Induced genomic instability in the human B lymphoblastoid cell line TK6 manifests itself as increases in end-to-end chromosome fusions and non-reciprocal chromosome translocations. It is not associated with elevated frequencies of specific locus mutations or other cytogenetic alterations. Previous studies on a limited...

journal_title：Mutagenesis

authors： Schwartz JL,Jordan R,Evans HH,Lenarczyk M,Liber HL

更新日期：2004-11-01 00:00:00

abstract：:The use of a white-ivory (wi) strain of Drosophila melanogaster carrying four copies of this allele, (wi)4, has proved to be useful in detecting somatic mutation in genotoxicity testing. Nevertheless, until now very little information exists about the nature of the genetic effects detected in such a strain. This work ...

journal_title：Mutagenesis

authors： Suárez S,Cabré O,Velázquez A,Marcos R,Xamena N

更新日期：1999-03-01 00:00:00

abstract：:A substantial database on hprt mutant frequency in circulating T-cells derived from 153 normal humans was analysed to determine if any effect of alcohol consumption could be detected. Alcohol intake (as reported in a questionnaire completed at the time of the blood sample) ranged from 0 to 35+ units per week. Donors w...

journal_title：Mutagenesis

authors： Cole J,Green MH

更新日期：1995-09-01 00:00:00