Baseline levels of chromosome instability in the human lymphoblastoid cell TK6.

abstract：:We have shown previously that UVC irradiation of benzo[a]pyrene diol epoxide (BPDE)-adducted DNA (BPDE/UVC) induces an increase in mutation frequency in the supF gene greater than the calculated additive value derived from either treatment alone, with a greater absolute increase in the level of BPDE signature transver...

journal_title：Mutagenesis

authors： McLuckie KI,Gaskell M,Farmer PB,Martin EA,Jones GD,Routledge MN

更新日期：2004-03-01 00:00:00

abstract：:In a sample of healthy subjects (23-59 years old), comprising 49 non-smokers and 23 smokers, we studied the effects of smoking on DNA repair synthesis (UDS) in peripheral lymphocytes. DNA repair after UV-irradiation was measured by [3H]TdR incorporation into DNA (semi-conservative synthesis was inhibited by hydroxyure...

journal_title：Mutagenesis

authors： Celotti L,Furlan D,Ferraro P,Levis AG

更新日期：1989-03-01 00:00:00

abstract：:This study investigated the effect of glucose and fructose, and advanced glycation end-products (AGEs) on genome damage in WIL2-NS cells, measured using the cytokinesis-block micronucleus cytome (CBMN-Cyt) assay. The effect of AGEs was investigated using the bovine serum albumin (AGE-BSA) model system induced either w...

journal_title：Mutagenesis

authors： Deo P,McCullough CL,Almond T,Jaunay EL,Donnellan L,Dhillon VS,Fenech M

更新日期：2020-03-27 00:00:00

abstract：:In the past decade, a number of case-control studies have been carried out to investigate the relationship between the HHEX polymorphism and type 2 diabetes (T2D). However, the results have been inconclusive. To investigate this inconsistency, we performed a meta-analysis of all available studies dealing with the rela...

journal_title：Mutagenesis

authors： Cai Y,Yi J,Ma Y,Fu D

更新日期：2011-03-01 00:00:00

abstract：:Neurocysticercosis (NCC) has been associated with a high frequency of DNA damage in human circulating lymphocytes and more recently with the development of hematological malignancies. Chronic inflammation, a common feature of helminthic infections, has been proposed to play a key role in carcinogenesis induced by para...

journal_title：Mutagenesis

authors： Herrera LA,Rodríguez U,Gebhart E,Ostrosky-Wegman P

更新日期：2001-11-01 00:00:00

abstract：:The aim of the present work was to investigate the processes involved in the origin of trisomic karyotypes, i.e. co-migration of sister chromatids (mitotic non-disjunction, MND) and recovery of micronuclei (MN) originating from lagging chromosomes/chromatids at anaphase (mitotic indirect non-disjunction, MIND), and to...

journal_title：Mutagenesis

authors： Minissi S,Degrassi F,Tanzarella C,Gustavino B

更新日期：1999-11-01 00:00:00

abstract：:Protection from chronic exposure to cosmic radiation, which is primarily composed of protons, in future manned missions to Mars and beyond is considered to be a key unresolved issue. To model the effects of cosmic radiation on a living cell, we used Saccharomyces cerevisiae cells harboring various deletions of DNA rep...

journal_title：Mutagenesis

authors： Rostek C,Turner EL,Robbins M,Rightnar S,Xiao W,Obenaus A,Harkness TA

更新日期：2008-03-01 00:00:00

abstract：:The spectrum of mutations induced by cobalt(II) chloride (CoCl2) was examined using plasmid pUB3 DNA, which was propagated after transfection into Escherichia coli SY1032/pKY241 host cells. The vector plasmid carried an E.coli supF suppressor tRNA gene as a target for mutations. After CoCl2 treatment, 64 independent n...

journal_title：Mutagenesis

authors： Ogawa HI,Ohyama Y,Ohsumi Y,Kakimoto K,Kato Y,Shirai Y,Nunoshiba T,Yamamoto K

更新日期：1999-03-01 00:00:00

abstract：:Cyclooxygenase 2 (Cox-2, a rate-limiting enzyme in the conversion of arachidonic acid to prostanoids) has been implicated in several physiological and pathological processes, and it has been reported that polymorphisms in the regulatory region of Cox-2 might influence its expression, contributing to the interindividua...

journal_title：Mutagenesis

authors： Tang Z,Nie ZL,Pan Y,Zhang L,Gao L,Zhang Q,Qu L,He B,Song G,Zhang Y,Shukui Wang

更新日期：2011-11-01 00:00:00

abstract：:Primary liver fibroblasts were applied in a cytokinesis-block micronucleus assay in combination with fluorescence in situ hybridization (FISH) using two protocols. In protocol A (Prot. A), cytochalasin B (Cyt B) was added at the end of the treatment time directly to the medium containing the standard compounds, wherea...

journal_title：Mutagenesis

authors： Nesti C,Trippi F,Scarpato R,Migliore L,Turchi G

更新日期：2000-03-01 00:00:00

abstract：:The lines irs1, irs2 and irs3, derived from V79-4 hamster cells, are sensitive to DNA-damaging agents including ionizing radiations. However, unlike some other radiosensitive lines, the irs lines show no apparent defect in the repair of DNA strand breaks. We have now assessed the mis-repair of DNA damage in the irs li...

journal_title：Mutagenesis

authors： Thacker J,Ganesh AN,Stretch A,Benjamin DM,Zahalsky AJ,Hendrickson EA

更新日期：1994-03-01 00:00:00

abstract：:Radiation-induced exchange aberrations are thought to arise as a consequence of misrejoining of free ends of DNA double strand breaks (dsbs). In quiescent mammalian cells this process of misrejoining is prevalently taken up by the non-homologous end joining (NHEJ) process. In order to investigate the role of glutathio...

journal_title：Mutagenesis

authors： Dutta A,Chakraborty A,Saha A,Ray S,Chatterjee A

更新日期：2005-09-01 00:00:00

abstract：:Black tea theafulvins, a fraction of thearubigins isolated from black tea aqueous infusions, potentiated the mutagenic activity of the mycotoxin aflatoxin B(1) in the Ames test, in the presence of a hepatic S9 activation system derived from Aroclor 1254-treated rats. In contrast, when the S9 activation system was repl...

journal_title：Mutagenesis

authors： Catterall F,Copeland E,Clifford MN,Ioannides C

更新日期：2003-03-01 00:00:00

abstract：:The aim of the present work was to investigate the possible interference of cytochalasin B (cyt B) with low concentration treatment with colchicine in the induction of chromosome/chromatid loss and micronuclei in human lymphocytes mitotically activated in vitro. Thus, cells from a single female donor were treated with...

journal_title：Mutagenesis

authors： Minissi S,Gustavino B,Degrassi F,Tanzarella C,Rizzoni M

更新日期：1999-01-01 00:00:00

abstract：:Hepatic microsomes from rats pretreated with PCB were found to metabolize the food mutagen 2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine (PhIP) to two major metabolites, one of which was identified as the N-hydroxy derivative, 2-hydroxy-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine (N-OH-PhIP). This identification w...

journal_title：Mutagenesis

authors： Frandsen H,Rasmussen ES,Nielsen PA,Farmer P,Dragsted L,Larsen JC

更新日期：1991-01-01 00:00:00

abstract：:A relatively large, reproducible inter-individual variation was found in the ability of 17 human liver S9 samples to mediate the mutagenicity of 2-acetylaminofluorene (2AAF) in Salmonella typhimurium strain TA98. In an animal model, variation in metabolic activation of 2AAF did not appear to relate to the phenotype of...

journal_title：Mutagenesis

authors： Smith AJ,Chipman JK

更新日期：1988-07-01 00:00:00

abstract：:Recombinant lambda phage DNA containing segments of human O6-methylguanine-DNA transferase gene was employed to localize this gene among the human chromosomes using non-radioactive in situ hybridization technique. This gene was found to be present at the telomeric end, 26q, of the long arm of the chromosome 10. ...

journal_title：Mutagenesis

authors： Natarajan AT,Vermeulen S,Darroudi F,Valentine MB,Brent TP,Mitra S,Tano K

更新日期：1992-01-01 00:00:00

abstract：:Chinese hamster ovary cells were exposed to N-hydroxy-2-aminofluorene, N-hydroxy-N'-acetylbenzidine and 1-nitrosopyrene, and the resulting DNA adducts, sister-chromatid exchanges (SCEs) and mutations at the hypoxanthine-guanine phosphoribosyl transferase locus were quantified. Each agent produced a major DNA adduct su...

journal_title：Mutagenesis

authors： Heflich RH,Morris SM,Beranek DT,McGarrity LJ,Chen JJ,Beland FA

更新日期：1986-05-01 00:00:00

abstract：:Fanconi anaemia (FA) is a rare autosomal recessive disease characterized by increased spontaneous and DNA crosslinker-induced chromosome instability, progressive pancytopenia and cancer susceptibility. An increasing number of genes are involved in FA, including the breast cancer susceptibility gene BRCA2. Five of the ...

journal_title：Mutagenesis

authors： Bogliolo M,Cabré O,Callén E,Castillo V,Creus A,Marcos R,Surrallés J

更新日期：2002-11-01 00:00:00

abstract：:A pronase digestion procedure for the isolation of gastric mucosal cells was evaluated for its usefulness in measuring unscheduled DNA synthesis (UDS). The method has been claimed to be suited for assessing the genotoxicity potential of compounds. Compounds were given orally to rats. After 13 h [3H]thymidine was injec...

journal_title：Mutagenesis

authors： Larsson H,Fryklund J,Helander HF,Wallmark B

更新日期：1991-01-01 00:00:00

abstract：:The development of novel nanomaterials with unique physico-chemical properties is increasing at a rapid rate, with potential applications across a broad range of manufacturing industries and consumer products. Nanomaterial safety is therefore becoming an increasingly contentious issue that has intensified over the pas...

journal_title：Mutagenesis

authors： Doak SH,Griffiths SM,Manshian B,Singh N,Williams PM,Brown AP,Jenkins GJ

更新日期：2009-07-01 00:00:00

abstract：:Macrocyclic polyethers (crown ethers) are a family of compounds which possess the ability to transport ionic species across natural and artificial membranes. Because of this characteristic, they have wide applications in industry and are being investigated for their potential as pharmacologic agents. However, these co...

journal_title：Mutagenesis

authors： Arenaz P,Bitticks L,Pannell K,Garcia S

更新日期：1989-11-01 00:00:00

abstract：:Radiation-induced bystander effects have been evaluated extensively, including the involvement of the mitogen-activated protein kinase (MAPK) pathways. However, few studies have examined the ability of chemicals to induce bystander effects, and the molecular mechanisms involved in chemical bystander effects have not b...

journal_title：Mutagenesis

authors： Asur R,Balasubramaniam M,Marples B,Thomas RA,Tucker JD

更新日期：2010-05-01 00:00:00

abstract：:We employed the three-way differential staining technique (TWD), which allows SCEs to be distinguished on a per generation basis by scoring third metaphases (M3), in order to study the spontaneous levels of SCEs in normal and high frequency cells (HFCs) that occurred in the first (S1), second (S2) and third (S3) S pha...

journal_title：Mutagenesis

authors： Ponzanelli I,Landi S,Bernacchi F,Barale R

更新日期：1997-09-01 00:00:00

abstract：:Among the tests used to determine the mutagenic potential of chemicals, the chromosomal aberrations and micronucleus assays play an important role. These tests score either chromosomal structural aberrations at metaphase or micronuclei at interphase. One of the hallmarks of apoptosis is DNA fragmentation into 50-300 k...

journal_title：Mutagenesis

authors： Meintières S,Biola A,Pallardy M,Marzin D

更新日期：2001-05-01 00:00:00

abstract：:A simplified method, using recombinant interleukin-2, foetal bovine serum and freeze-killed feeder cells, has been developed for the mass culture of T-lymphocytes derived from human peripheral blood. In this protocol, bulk cultures can be cryopreserved approximately 8 days after initiation, and subsequent mass culture...

journal_title：Mutagenesis

authors： O'Donovan MR,Freemantle MR,Hull G,Bell DA,Arlett CF,Cole J

更新日期：1995-05-01 00:00:00

abstract：:The repairability during G(1) of DNA lesions eliciting sister chromatid exchanges (SCE) induced by methylmethanesulfonate (MMS) and ethylmethanesulfonate (EMS) in BrdU-substituted and unsubstituted DNA strands was determined in murine salivary gland cells in vivo. The SCE frequency was determined after exposure to MMS...

journal_title：Mutagenesis

authors： González-Beltrán F,Morales-Ramírez P

更新日期：2003-01-01 00:00:00

abstract：:There is a correlation between intestinal cancer and diets high in meat, so fried beef, chicken, lamb, pork and fish were tested for their ability to induce mutations in the small intestine of mice. The mice were bred to be heterozygous at the Dlb-1 locus so that loss of the dominant Dlb-1 b allele by mutation could b...

journal_title：Mutagenesis

authors： Heddle JA,Knize MG,Dawod D,Zhang XB

更新日期：2001-03-01 00:00:00

abstract：:FD&C Red No.3 (erythrosine) has been used as a dye in foods, drugs and cosmetics since its approval by the US Department of Agriculture in 1907. In 1977 the Certified Color Manufacturers' Association (CCMA) initiated studies on FD&C Red No.3 including chronic toxicity and carcinogenicity studies in rats and mice. Data...

journal_title：Mutagenesis

authors： Lin GH,Brusick DJ

更新日期：1986-07-01 00:00:00

abstract：:The single-cell gel electrophoresis--the comet assay--has proved to be a sensitive and relatively simple method that is much used in research for the analysis of specific types of DNA damage, and its use in genotoxicity testing is increasing. The efficiency of the comet assay, in terms of number of samples processed p...

journal_title：Mutagenesis

authors： Gutzkow KB,Langleite TM,Meier S,Graupner A,Collins AR,Brunborg G

更新日期：2013-05-01 00:00:00