Abstract:
:The single-cell gel electrophoresis--the comet assay--has proved to be a sensitive and relatively simple method that is much used in research for the analysis of specific types of DNA damage, and its use in genotoxicity testing is increasing. The efficiency of the comet assay, in terms of number of samples processed per experiment, has been rather poor, and both research and toxicological testing should profit from an increased throughput. We have designed and validated a format involving 96 agarose minigels supported by a hydrophilic polyester film. Using simple technology, hundreds of samples may be processed in one experiment by one person, with less time needed for processing, less use of chemicals and requiring fewer cells per sample. Controlled electrophoresis, including circulation of the electrophoresis solution, improves the homogeneity between replicate samples in the 96-minigel format. The high-throughput method described in this paper should greatly increase the overall capacity, versatility and robustness of the comet assay.
journal_name
Mutagenesisjournal_title
Mutagenesisauthors
Gutzkow KB,Langleite TM,Meier S,Graupner A,Collins AR,Brunborg Gdoi
10.1093/mutage/get012subject
Has Abstractpub_date
2013-05-01 00:00:00pages
333-40issue
3eissn
0267-8357issn
1464-3804pii
get012journal_volume
28pub_type
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