Association between age and repair of oxidatively damaged DNA in human peripheral blood mononuclear cells.


:It has been hypothesised that positive associations between age and levels of oxidative stress-generated damage to DNA may be related to an age-dependent decline in DNA repair activity. The objective of this study was to investigate the association between age and repair activity of oxidatively damaged DNA in peripheral blood mononuclear cells (PBMCs). We isolated PBMCs from subjects aged 18-83 years, as part of a health survey of the Danish population that focussed on lifestyle factors. The level of DNA repair activity was measured as incisions on potassium bromate-damaged DNA by the comet assay. There was an inverse association between age and DNA repair activity with a 0.65% decline in activity per year from age 18 to 83 (95% confidence interval: 0.16-1.14% per year). Univariate regression analysis also indicated inverse associations between DNA repair activity and waist-hip ratio (P < 0.05) and plasma concentrations of glycosylated hemoglobin (P = 0.07). However, multivariate regression analysis only showed an inverse association between age and DNA repair activity (P < 0.05), indicating that the decline in repair activity was not mediated by metabolic risk factors. In summary, the results show an inverse association between age and DNA repair activity of oxidatively damaged DNA.






Løhr M,Jensen A,Eriksen L,Grønbæk M,Loft S,Møller P




Has Abstract


2015-09-01 00:00:00














  • Micronucleus morphology as a means to distinguish aneugens and clastogens in the mouse bone marrow micronucleus assay.

    abstract::Detailed dose-response data for two reference aneugens, vincristine and nocodazole, have been derived for the mouse bone marrow micronucleus assay. Positive data have also been described for six other micronucleus-inducing agents: cyclophosphamide; aniline; urethane; 1,2-dimethylhydrazine; 7,12-dimethylbenzanthracene ...


    pub_type: 杂志文章


    authors: Tinwell H,Ashby J

    更新日期:1991-05-01 00:00:00

  • The involvement of chromatin condensation in camptothecin-induced chromosome breaks in G0 human lymphocytes.

    abstract::In the present study we evaluated campthotecin (CPT)-induced chromosomal damage in human lymphocytes in the G0 phase of the cell cycle as revealed by the premature chromosome condensation technique. The results obtained here indicate that CPT was able to induce chromosome fragments in the G0 phase of the cell cycle of...


    pub_type: 杂志文章


    authors: Mosesso P,Fonti E,Bassi L,Lorenti Garcia C,Palitti F

    更新日期:1999-01-01 00:00:00

  • Mutagenicity studies on FD&C red No.3.

    abstract::FD&C Red No.3 (erythrosine) has been used as a dye in foods, drugs and cosmetics since its approval by the US Department of Agriculture in 1907. In 1977 the Certified Color Manufacturers' Association (CCMA) initiated studies on FD&C Red No.3 including chronic toxicity and carcinogenicity studies in rats and mice. Data...


    pub_type: 杂志文章


    authors: Lin GH,Brusick DJ

    更新日期:1986-07-01 00:00:00

  • The detection of genotoxin-induced DNA adducts in the common mussel Mytilus edulis.

    abstract::In order to establish the capacity of Mytilus spp. to form genotoxin-DNA adducts, a series of in vitro and in vivo studies were conducted in which tissue samples and animals were exposed to five model genotoxins. Following the in vitro characterization of the major adducts induced by the compounds, a series of in vivo...


    pub_type: 杂志文章


    authors: Harvey JS,Parry JM

    更新日期:1997-05-01 00:00:00

  • Genetic polymorphisms of genes involved in DNA repair and metabolism influence micronucleus frequencies in human peripheral blood lymphocytes.

    abstract::The cytokinesis-block micronucleus cytome (CBMNCyt) assay is a widely used technique for measuring DNA damage in human populations. The formation of micronuclei (MN) in dividing cells can result from chromosome breakage due to unrepaired or mis-repaired DNA lesions or chromosome malsegregation due to mitotic malfuncti...


    pub_type: 杂志文章,评审


    authors: Dhillon VS,Thomas P,Iarmarcovai G,Kirsch-Volders M,Bonassi S,Fenech M

    更新日期:2011-01-01 00:00:00

  • Interaction of radiation- and bleomycin-induced lesions and influence of glutathione level on the interaction.

    abstract::Radiation-induced exchange aberrations are thought to arise as a consequence of misrejoining of free ends of DNA double strand breaks (dsbs). In quiescent mammalian cells this process of misrejoining is prevalently taken up by the non-homologous end joining (NHEJ) process. In order to investigate the role of glutathio...


    pub_type: 杂志文章


    authors: Dutta A,Chakraborty A,Saha A,Ray S,Chatterjee A

    更新日期:2005-09-01 00:00:00

  • Pms2 deficiency results in increased mutation in the Hprt gene but not the Tk gene of Tk(+/-) transgenic mice.

    abstract::The effects of deficiency in the DNA mismatch repair (MMR) protein Pms2 were investigated using the endogenous mouse Hprt and Tk genes as reporters of intragenic mutation and loss of heterozygosity (LOH). Pms2(-/-)Tk(+/-), Pms2(+/+)Tk(+/-), Pms2(+/-)Tk(+/-) and Pms2(-/-)Tk(-/-) mice were bred from Pms2(+/-)Tk(+/-) mic...


    pub_type: 杂志文章


    authors: Dobrovolsky VN,McKinzie PB,Shaddock JG,Mittelstaedt RA,Heflich RH,Parsons BL

    更新日期:2003-07-01 00:00:00

  • Investigation of the effects of DNA repair gene polymorphisms on the risk of colorectal cancer.

    abstract::Despite their prime candidate status, polymorphisms near genes involved in DNA repair or in other functions related to genome stability have been conspicuously under-represented in the significant associations reported from genome-wide association studies (GWAS) of cancer susceptibility. In this study, we assessed a s...


    pub_type: 杂志文章


    authors: Tomlinson IP,Houlston RS,Montgomery GW,Sieber OM,Dunlop MG

    更新日期:2012-03-01 00:00:00

  • Effect of cytochalasin B on the induction of chromosome missegregation by colchicine at low concentrations in human lymphocytes.

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    pub_type: 杂志文章


    authors: Minissi S,Gustavino B,Degrassi F,Tanzarella C,Rizzoni M

    更新日期:1999-01-01 00:00:00

  • Induction of hepatic mutations in lacI transgenic mice.

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    pub_type: 杂志文章


    authors: Mirsalis JC,Provost GS,Matthews CD,Hamner RT,Schindler JE,O'Loughlin KG,MacGregor JT,Short JM

    更新日期:1993-05-01 00:00:00

  • A modified protocol for the cytochalasin B in vitro micronucleus assay using whole human blood or separated lymphocyte cultures.

    abstract::A modified protocol is described for the in vitro analysis of micronuclei in whole blood or separated lymphocyte cultures. The induction of binucleate cells by various concentrations of cytochalasin B (3, 4.5 or 6 micrograms/ml) was examined at two harvest times (68 or 72 h). An optimal yield was obtained by adding cy...


    pub_type: 杂志文章


    authors: Ellard S,Parry EM

    更新日期:1993-07-01 00:00:00

  • Molecular analysis of mutants obtained by treatment with alkylating agents in a quadruplicated white-ivory strain of Drosophila melanogaster.

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    pub_type: 杂志文章


    authors: Suárez S,Cabré O,Velázquez A,Marcos R,Xamena N

    更新日期:1999-03-01 00:00:00

  • Oxidative damage to DNA by diesel exhaust particle exposure in co-cultures of human lung epithelial cells and macrophages.

    abstract::Studies in mono-culture of cells have shown that diesel exhaust particles (DEPs) increase the production of reactive oxygen species (ROS) and oxidative stress-related damage to DNA. However, the level of particle-generated genotoxicity may depend on interplay between different cell types, e.g. lung epithelium and immu...


    pub_type: 杂志文章


    authors: Jantzen K,Roursgaard M,Desler C,Loft S,Rasmussen LJ,Møller P

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  • CI solvent yellow 14 shows activity in the bone marrow micronucleus assay in both the rat and mouse.

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    pub_type: 杂志文章


    authors: Elliott BM,Griffiths K,Mackay JM,Wade JD

    更新日期:1997-07-01 00:00:00

  • Aristolochic acid as a probable human cancer hazard in herbal remedies: a review.

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    pub_type: 杂志文章,评审


    authors: Arlt VM,Stiborova M,Schmeiser HH

    更新日期:2002-07-01 00:00:00

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    pub_type: 杂志文章


    authors: Khan MS,Pandith AA,Azad N,Hussain MU,Masoodi SR,Wani KA,Andrabi KI,Mudassar S

    更新日期:2014-03-01 00:00:00

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    pub_type: 杂志文章


    authors: Sun F,Yin H,Eichenlaub-Ritter U

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  • Characterization of SV40-transformed xeroderma pigmentosum cell lines for their usability in HPRT mutation studies.

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    pub_type: 杂志文章


    authors: Merk O,Speit G

    更新日期:1997-09-01 00:00:00

  • Gene expression profiling in primary mouse hepatocytes discriminates true from false-positive genotoxic compounds.

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    pub_type: 杂志文章


    authors: Mathijs K,Brauers KJ,Jennen DG,Lizarraga D,Kleinjans JC,van Delft JH

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  • DNA damage and repair of leukocytes from Fanconi anaemia patients, carriers and healthy individuals as measured by the alkaline comet assay.

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    pub_type: 杂志文章


    authors: Mohseni-Meybodi A,Mozdarani H,Mozdarani S

    更新日期:2009-01-01 00:00:00

  • Detection of chemical mutagens using Muta Mouse: a transgenic mouse model.

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    pub_type: 杂志文章


    authors: Hoorn AJ,Custer LL,Myhr BC,Brusick D,Gossen J,Vijg J

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  • Assessment of DNA damage in underground coal miners using the cytokinesis-block micronucleus assay in peripheral blood lymphocytes.

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    pub_type: 杂志文章


    authors: Sinitsky MY,Minina VI,Gafarov NI,Asanov MA,Larionov AV,Ponasenko AV,Volobaev VP,Druzhinin VG

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    pub_type: 杂志文章


    authors: van Buul PP,Seelen CM,Schöppink K,Goudzwaard JH

    更新日期:1992-07-01 00:00:00

  • Validation of the in vivo CD1 mouse splenocyte micronucleus test.

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    pub_type: 杂志文章


    authors: Benning V,Brault D,Duvinage C,Thybaud V,Melcion C

    更新日期:1994-05-01 00:00:00

  • Exosomal microRNAs and other non-coding RNAs as colorectal cancer biomarkers: a review.

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    pub_type: 杂志文章


    authors: Francavilla A,Turoczi S,Tarallo S,Vodicka P,Pardini B,Naccarati A

    更新日期:2020-07-11 00:00:00

  • DNA damage and DNA damage response in human bronchial epithelial BEAS-2B cells following exposure to 2-nitrobenzanthrone and 3-nitrobenzanthrone: role in apoptosis.

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    pub_type: 杂志文章


    authors: Oya E,Ovrevik J,Arlt VM,Nagy E,Phillips DH,Holme JA

    更新日期:2011-11-01 00:00:00

  • Biological dose estimation of UVA laser microirradiation utilizing charged particle-induced protein foci.

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    pub_type: 杂志文章


    authors: Splinter J,Jakob B,Lang M,Yano K,Engelhardt J,Hell SW,Chen DJ,Durante M,Taucher-Scholz G

    更新日期:2010-05-01 00:00:00

  • The Cox-2 -1195 G > A polymorphism and cancer risk: a meta-analysis of 25 case-control studies.

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    pub_type: 杂志文章,meta分析


    authors: Tang Z,Nie ZL,Pan Y,Zhang L,Gao L,Zhang Q,Qu L,He B,Song G,Zhang Y,Shukui Wang

    更新日期:2011-11-01 00:00:00

  • A comparison of the antimutagenic potential of green, black and decaffeinated teas: contribution of flavanols to the antimutagenic effect.

    abstract::The present study was undertaken to compare the antimutagenic activity of aqueous extracts, at the concentrations used for human consumption, from green, black and decaffeinated black tea. Antimutagenic potential was evaluated against three indirect-acting dietary carcinogens, Glu-P-1, benzo(a)pyrene and nitrosopyrrol...


    pub_type: 杂志文章


    authors: Bu-Abbas A,Nunez X,Clifford MN,Walker R,Ioannides C

    更新日期:1996-11-01 00:00:00

  • Haemoglobin adducts as biomarkers of occupational exposure to 1,3-butadiene.

    abstract::Adducts of 1,2-epoxy-3-butene (EB) with haemoglobin were monitored in 17 workers from the 1,3-butadiene (BD) production unit of a petrochemical plant and in nine referents employed at the same factory but not exposed to BD. The air concentrations of BD were determined using stationary and personal monitoring. The ambi...


    pub_type: 杂志文章


    authors: Osterman-Golkar S,Peltonen K,Anttinen-Klemetti T,Landin HH,Zorcec V,Sorsa M

    更新日期:1996-03-01 00:00:00