Abstract:
:Recent problems with the supply of Difco bacto agar have forced some laboratories to evaluate alternative agars for use in the Salmonella/microsome assay. This led to the independent observation in two laboratories (Boots and Glaxo) that Salmonella typhimurium TA97a is sensitive to certain types of agar that may be used to prepare Vogel-Bonner minimal medium plates. A programme of work was, therefore, undertaken to investigate this phenomenon; 9-aminoacridine hydrochloride (at Boots) and 4-nitro-o-phenylenediamine (at Glaxo) were tested against TA1537 and TA97a using Vogel-Bonner plates prepared with a number of different agars. Three agars (Lab M, Difco Bi-tek and Beckton Dickinson granulated) were identified which, although supporting normal growth of TA1537 revertant colonies, gave much reduced control counts and responses to the mutagens with TA97a. One agar, Becton Dickinson grade A, gave poor responses with TA1537 but produced satisfactory results with TA97a. In contrast to the Vogel-Bonner plates, varying the type of agar used in the top agar overlays had little effect on the responses obtained. On the basis of these comparisons, Becton Dickinson purified agar was selected as a suitable alternative to Difco bacto and it was concluded that laboratories using agars other than these, or purchasing pre-poured plates without specifying the type of agar, should be made aware of potential problems with TA97a.
journal_name
Mutagenesisjournal_title
Mutagenesisauthors
Wilcox P,Wedd DJ,Williams WR,Mee CD,O'Donovan MRdoi
10.1093/mutage/7.1.13subject
Has Abstractpub_date
1992-01-01 00:00:00pages
13-8issue
1eissn
0267-8357issn
1464-3804journal_volume
7pub_type
杂志文章相关文献
MUTAGENESIS文献大全abstract::The genotoxicity of alpha-hexachlorocyclohexane (alpha-HCH) was evaluated in primary cultures of mouse, rat and human hepatocytes. DNA fragmentation was measured by the alkaline elution technique and DNA repair synthesis by quantitative autoradiography. A 20 h exposure to subtoxic concentrations ranging from 0.056 to ...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/11.1.79
更新日期:1996-01-01 00:00:00
abstract::The presence of an adaptive response to low doses of alkylating chemicals in the phototrophic bacteria Rhodobacter sphaeroides and R.capsulatus has been studied. Results obtained show that both strains display this repair response against the challenge doses of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), when they ar...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/3.2.165
更新日期:1988-03-01 00:00:00
abstract::Anaesthetics have gained a lot of attention for their potential mutagenic/carcinogenic effects. In the present study we have investigated the genotoxicity of the inhalation anaesthetic sevoflurane on DNA of lymphocytes isolated from 20 patients undergoing orthopaedic surgery. The genotoxicity of the anaesthetic was st...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/geg013
更新日期:2003-09-01 00:00:00
abstract::Published data regarding the association between the apurinic/apyrimidinic endonuclease 1 (APE1) T1349G (Asp148Glu) polymorphism and cancer risk show inconclusive results. To derive a more precise estimation of the relationship, we performed a meta-analysis of 27 published studies that included 12 432 cancer cases and...
journal_title:Mutagenesis
pub_type: 杂志文章,meta分析
doi:10.1093/mutage/gep036
更新日期:2009-11-01 00:00:00
abstract::Urine and serum samples of rats bearing three different experimental tumours (hepatocellular carcinoma, myelomonocytic leukemia and mesoblastic nephroma) were investigated for mutagenicity with the Ames Salmonella test. Enhancement of mutagenic activity in TA98 and TA100 was observed only in the case of urine samples ...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/10.1.43
更新日期:1995-01-01 00:00:00
abstract::The tetrahydrofuran-type abasic site analog (THF) induces large deletion mutations in human cells. To compare the large deletions induced by THF on leading and lagging strand templates, plasmid DNAs bearing the analog at a specific position outside the supF gene were introduced into human U2OS cells. The replicated DN...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gez034
更新日期:2019-12-19 00:00:00
abstract::Aneuploidy of mouse chromosome 8 induced by a Chinese medicinal herb, Tripterygium hypoglaucum (level) Hutch (THH) was investigated by fluorescence in situ hybridization (FISH) in vivo. Male mice were treated with THH (single i.p. injection) at doses of 120, 240 and 480 mg/kg. Colchicine (COL, 1.5 mg/kg i.p.) was used...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/geh044
更新日期:2004-09-01 00:00:00
abstract::We have conducted a randomized trial which investigated the ability of dietary changes (in particular diets rich in cruciferous vegetables and flavonoids), to increase urinary antimutagenicity and inhibit DNA damage in smokers. Ninety heavy smokers were recruited and randomly assigned to three groups, and were given t...
journal_title:Mutagenesis
pub_type: 杂志文章,随机对照试验
doi:10.1093/mutage/gel021
更新日期:2006-05-01 00:00:00
abstract::A substantial database on hprt mutant frequency in circulating T-cells derived from 153 normal humans was analysed to determine if any effect of alcohol consumption could be detected. Alcohol intake (as reported in a questionnaire completed at the time of the blood sample) ranged from 0 to 35+ units per week. Donors w...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/10.5.449
更新日期:1995-09-01 00:00:00
abstract::Nanoremediation of soil, ground and surface water using nanoscale zerovalent iron particles (nZVI) has facilitated their direct environmental exposure posing ecotoxicological concerns. Numerous studies elucidate their phytotoxicity in terms of growth and their fate within the plant system. However, their potential gen...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gex006
更新日期:2017-05-01 00:00:00
abstract::The old herbal drug aristolochic acid (AA), derived from Aristolochia spp., has been associated with the development of a novel nephropathy, designated aristolochic acid nephropathy (AAN), and urothelial cancer in AAN patients. There is clear evidence that the major components of the plant extract AA, aristolochic aci...
journal_title:Mutagenesis
pub_type: 杂志文章,评审
doi:10.1093/mutage/17.4.265
更新日期:2002-07-01 00:00:00
abstract::Well-established in vitro methods for testing the genotoxic potency of chemicals--such as the Ames/Salmonella test, the mouse lymphoma assay, the micronucleus test and the chromosomal aberration test--show a high false-positive rate for predicting in vivo genotoxicity and carcinogenicity. Thus, there is a need for mor...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/geq040
更新日期:2010-11-01 00:00:00
abstract::Studies in mono-culture of cells have shown that diesel exhaust particles (DEPs) increase the production of reactive oxygen species (ROS) and oxidative stress-related damage to DNA. However, the level of particle-generated genotoxicity may depend on interplay between different cell types, e.g. lung epithelium and immu...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/ges035
更新日期:2012-11-01 00:00:00
abstract:UNLABELLED:Gastric cancer is the second leading cause of cancer-related death worldwide with a low 5-year survival (S5y) after initial diagnosis. Although aberrant Wnt/β-catenin (CTNNB1) signaling has been observed in multiple human cancers, there is no information on the role of CTNNB1 polymorphisms in gastric cancer ...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/ges027
更新日期:2012-11-01 00:00:00
abstract::Basic extracts isolated from lamb meat treated at various temperatures were tested for mutagenicity in Salmonella typhimurium strain TA98 in the presence of S9 mix. Samples of ground lamb patties were cooked for 10 min per side at 100, 150, 200, 250 and 300 degrees C. Low cooking temperatures resulted in products with...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/4.3.228
更新日期:1989-05-01 00:00:00
abstract::o-Phenylphenol (OPP), a widely used fungicide and antibacterial agent, has been considered to be among the top 10 home and garden pesticides used in the USA. Earlier studies have consistently shown that the sodium salt of OPP (SOPP) causes bladder cancer in male Fischer 344 (F344) rats, whereas OPP has produced variab...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/17.1.89
更新日期:2002-01-01 00:00:00
abstract::Type 2 diabetes mellitus has undergone a worldwide growth in incidence in the world and has now acquired epidemic status. There is a strong link between type 2 diabetes and vitamin D deficiency. Because vitamin D has beneficial effects on glucose homeostasis, the aim of this study was to evaluate the influence of vita...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gez001
更新日期:2019-05-29 00:00:00
abstract::Niclosamide produces genotoxic effects, such as point mutations in Salmonella sp., sperm-head abnormalities in mice and clastogenic effects in human lymphocytes in vitro and in vivo. As cytochrome P450 could be involved in the bioactivation of niclosamide, we investigated which subfamily was involved. We used liver mi...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/get043
更新日期:2013-11-01 00:00:00
abstract::Zeocin is a member of the bleomycin/phleomycin family of antibiotics, known to bind and cleave DNA. We established human SK-OV-3 cells that stably express the Zeocin resistance gene (Sh ble) using an ecdysone-inducible mammalian expression system. Surprisingly, our results demonstrated that Zeocin, added in the cultur...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gei016
更新日期:2005-03-01 00:00:00
abstract::The principal hydroxy-metabolites of benzene--hydroquinone, catechol and phenol--were assayed in tests for mitotic segregation induction in Aspergillus nidulans diploid strain 19. Hydroquinone was the most effective chemical, increasing the frequency of mitotic segregants up to 10-fold at 1-3 mM. Catechol was similarl...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/2.3.235
更新日期:1987-05-01 00:00:00
abstract::A modified protocol is described for the in vitro analysis of micronuclei in whole blood or separated lymphocyte cultures. The induction of binucleate cells by various concentrations of cytochalasin B (3, 4.5 or 6 micrograms/ml) was examined at two harvest times (68 or 72 h). An optimal yield was obtained by adding cy...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/8.4.317
更新日期:1993-07-01 00:00:00
abstract::Exposure to ambient air particles is associated with elevated levels of DNA strand breaks (SBs) and endonuclease III, formamidopyrimidine DNA glycosylase (FPG) and oxoguanine DNA glycosylase-sensitive sites in cell cultures, animals and humans. In both animals and cell cultures, increases in SB and in oxidatively dama...
journal_title:Mutagenesis
pub_type: 杂志文章,评审
doi:10.1093/mutage/geu035
更新日期:2015-01-01 00:00:00
abstract::As part of the third UKEMS collaborative trial, ICI Central Toxicology Laboratory (CTL) tested the three study chemicals ethylmethanesulphonate (EMS), benzo[a]pyrene (B[a]P) and benzidine (BZD) in a microwell adaptation of the L5178Y gene mutation assay using the thymidine kinase (tk) locus. Cofactors for metabolic ac...
journal_title:Mutagenesis
pub_type: 临床试验,杂志文章,多中心研究
doi:
更新日期:1990-01-01 00:00:00
abstract::This study investigated the effect of glucose and fructose, and advanced glycation end-products (AGEs) on genome damage in WIL2-NS cells, measured using the cytokinesis-block micronucleus cytome (CBMN-Cyt) assay. The effect of AGEs was investigated using the bovine serum albumin (AGE-BSA) model system induced either w...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/geaa002
更新日期:2020-03-27 00:00:00
abstract::Neurocysticercosis (NCC) has been associated with a high frequency of DNA damage in human circulating lymphocytes and more recently with the development of hematological malignancies. Chronic inflammation, a common feature of helminthic infections, has been proposed to play a key role in carcinogenesis induced by para...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/16.6.495
更新日期:2001-11-01 00:00:00
abstract::A systematic review of the literature has been conducted and studies reporting investigations of genotoxicity biomarkers in pesticide workers have been assessed with view to establishing whether there was evidence for any risk to those using pesticides approved in the United Kingdom. Each of the studies was evaluated ...
journal_title:Mutagenesis
pub_type: 杂志文章,评审
doi:10.1093/mutage/gel011
更新日期:2006-03-01 00:00:00
abstract::Over the past several years, we have been evaluating the mutagenicity and clastogenicity of compounds capable of Michael-type reactions. These compounds, including acrylamide, several acrylate and methacrylate esters, vinyl sulfones, and phorone, have been evaluated using TK+/- -3.7.2C mouse lymphoma cells. Mutagenic ...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/6.6.519
更新日期:1991-11-01 00:00:00
abstract::The antineoplastic drug etoposide (ET) inhibits topoisomerase II (topo II) activity by forming a ternary complex (DNA-ET-topo II). This complex prevents the DNA-strand-rejoining activity of topo II and may result in structural chromosome aberrations. Inhibition of topo II activity may also predispose cells to aneuploi...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/11.4.357
更新日期:1996-07-01 00:00:00
abstract::Nitropyrenes are a class of polycyclic hydrocarbons that have been shown to be potent point mutagens in the Salmonella/mammalian microsome assay. However, relatively few data have been published on their ability to induce chromosome aberrations. In this paper we report the cytogenetic activity of 1-nitropyrene, 1-nitr...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/2.1.23
更新日期:1987-01-01 00:00:00
abstract::The human metabolome-the complement of small molecule metabolites present in biofluids and tissues-represents a significant part of the internal chemical milieu and is therefore an important aspect of the human exposome. Metabolic profiling approaches, commonly referred to as metabonomics or metabolomics, permit detai...
journal_title:Mutagenesis
pub_type: 杂志文章,评审
doi:10.1093/mutage/gev060
更新日期:2015-11-01 00:00:00