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Cell transformation assays for prediction of carcinogenic potential: state of the science and future research needs.

Abstract：

:Cell transformation assays (CTAs) have long been proposed as in vitro methods for the identification of potential chemical carcinogens. Despite showing good correlation with rodent bioassay data, concerns over the subjective nature of using morphological criteria for identifying transformed cells and a lack of understanding of the mechanistic basis of the assays has limited their acceptance for regulatory purposes. However, recent drivers to find alternative carcinogenicity assessment methodologies, such as the Seventh Amendment to the EU Cosmetics Directive, have fuelled renewed interest in CTAs. Research is currently ongoing to improve the objectivity of the assays, reveal the underlying molecular changes leading to transformation and explore the use of novel cell types. The UK NC3Rs held an international workshop in November 2010 to review the current state of the art in this field and provide directions for future research. This paper outlines the key points highlighted at this meeting.

journal_name

Mutagenesis

journal_title

Mutagenesis

authors

Creton S,Aardema MJ,Carmichael PL,Harvey JS,Martin FL,Newbold RF,O'Donovan MR,Pant K,Poth A,Sakai A,Sasaki K,Scott AD,Schechtman LM,Shen RR,Tanaka N,Yasaei H

doi

10.1093/mutage/ger053

subject

Has Abstract

pub_date

2012-01-01 00:00:00

pages

93-101

issue

1

eissn

0267-8357

issn

1464-3804

pii

ger053

journal_volume

27

pub_type

杂志文章

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