Abstract：
:Intracellular calcium oscillation caused by receptor activator of nuclear factor kappa-B ligand has been demonstrated to promote the differentiation of osteoclasts. Osteoclasts are recruited on the surface of trabeculae, and are exposed to fluid flow caused by the deformation of the bone matrix. However, the roles of fluid shear stress (FSS) on calcium response during the differentiation process of osteoclasts are still unknown. In the current study, the formation of tartrate-resistant acid phosphatase-positive, multinucleated osteoclasts from RAW264.7 macrophage cells were induced by co-culturing them with the conditioned medium from MC3T3-E1 osteoblasts. The in situ observations showed a high correlation between the area and the nuclear number of osteoclasts. The cells were stimulated by FSS at different levels (1 or 10 dyne/cm(2)) before (0 day) or after being induced for 4 or 8 days. The mechanically-induced calcium response was recorded and analyzed. The results indicated a different property of calcium oscillation for the osteoclasts in different fusion stages (i.e., more calcium-responsive peaks appeared in small osteoclasts than those in the larger ones). The rates of calcium influx decreased and the time of recovery in osteoclast cytosol increased along with the fusion of osteoclasts. In addition, increasing the FSS level enhanced the calcium oscillation of osteoclasts at early induction (4 days). However, this effect was weakened at the late induction (8 days). The present work could help provide understanding regarding the mechanism of the involvement of calcium in mechanically induced bone remodeling.
journal_name
Ann Biomed Engjournal_title
Annals of biomedical engineeringauthors
Li P,Hu M,Sun S,Zhang Y,Gao Y,Long M,Huo B,Zhang Ddoi
10.1007/s10439-012-0554-zsubject
Has Abstractpub_date
2012-09-01 00:00:00pages
1874-83issue
9eissn
0090-6964issn
1573-9686journal_volume
40pub_type
杂志文章abstract：:This paper presents the permeability analysis of neuroactive drugs and correlation with in vivo brain/plasma ratios in a dynamic microfluidic blood-brain barrier (BBB) model. Permeability of seven neuroactive drugs (Ethosuximide, Gabapentin, Sertraline, Sunitinib, Traxoprodil, Varenicline, PF-304014) and trans-endothe...
journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章,评审
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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