Abstract:
:Human immunodeficiency virus-1 (HIV-1) infection and the acquired immune deficiency syndrome (AIDS) pandemic remain global threats in the absence of a protective or a therapeutic vaccine. HIV-1 replication is reportedly inhibited by some cellular factors, including APOBEC3G (A3G) and APOBEC3F (A3F), which are well known inhibitors of HIV-1. Recently, HIV-1 Gag-virus-like particles (Gag-VLPs) have been shown to be safe and potent HIV-1 vaccine candidates that can elicit strong cellular and humoral immunity without need of any adjuvant. In this report, we stimulated human monocyte-derived dendritic cells (DCs) with Gag-VLPs and we demonstrated that Gag-VLP-treated DCs (VLP-DCs) produced interferon alpha (IFN-α), along with an increase in mRNA and protein expression of A3G and A3F. Gag-VLPs inhibited HIV-1 replication not only in DCs themselves, but also in cocultured T cells in an IFN-α-dependent manner. In addition, A3G/3F content in HIV virions released from VLP-DCs increased. Both the increase in A3G/3F expression and the inhibition of HIV-1 replication were reversed by anti-IFN-α or anti-IFNAR antibodies. Our findings in this study provide insight into the mechanism of Gag-VLP-induced inhibition of HIV-1 replication in DCs and T cells.
journal_name
J Innate Immunjournal_title
Journal of innate immunityauthors
Chang MO,Suzuki T,Yamamoto N,Watanabe M,Takaku Hdoi
10.1159/000339402subject
Has Abstractpub_date
2012-01-01 00:00:00pages
579-90issue
5-6eissn
1662-811Xissn
1662-8128pii
000339402journal_volume
4pub_type
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journal_title:Journal of innate immunity
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journal_title:Journal of innate immunity
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journal_title:Journal of innate immunity
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journal_title:Journal of innate immunity
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journal_title:Journal of innate immunity
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journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000317665
更新日期:2010-01-01 00:00:00
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journal_title:Journal of innate immunity
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doi:10.1159/000480386
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journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000439052
更新日期:2016-01-01 00:00:00
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journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000203700
更新日期:2009-01-01 00:00:00
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journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000446498
更新日期:2016-01-01 00:00:00
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journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000329226
更新日期:2012-01-01 00:00:00
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journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000339826
更新日期:2013-01-01 00:00:00