Abstract:
BACKGROUND:Coxiella burnetii, an obligate intracellular organism, is the causative agent of the zoonotic disease Q fever. The seroprevalence rate for Q fever in the United States is 3.1%, suggesting a high number of infections each year. However, fewer than 200 cases of Q fever are reported to the CDC annually. This discrepancy is likely the result of underutilized diagnostics and a high percentage (>50%) of asymptomatic infections. The detection of C. burnetii in patient blood during the first 2 to 3 weeks of infection raises the possibility that the organism could be present in donated human blood. The purpose of this study was to determine if extracellular C. burnetii would be stable in blood under normal storage conditions. STUDY DESIGN AND METHODS:Donated human blood was separated into whole blood, leukoreduced whole blood, red blood cells, and plasma. Each component was spiked with purified, extracellular C. burnetii strain Nine Mile Phase 1, and the viability and infectivity of the organisms were tested weekly. RESULTS:C. burnetii did not decrease in viability or the ability to infect cells after storage in any of the blood products, even after 6 weeks of storage at 1 to 6 °C. CONCLUSIONS:Extracellular C. burnetii can survive and remain infectious in donated blood products.
journal_name
Transfusionjournal_title
Transfusionauthors
Kersh GJ,Priestley R,Massung RFdoi
10.1111/j.1537-2995.2012.03912.xsubject
Has Abstractpub_date
2013-07-01 00:00:00pages
1493-6issue
7eissn
0041-1132issn
1537-2995journal_volume
53pub_type
杂志文章相关文献
TRANSFUSION文献大全abstract:BACKGROUND:It is important to compare the incidence of bacterial contamination of components collected from the peripheral blood or bone marrow (BM), as well as of components processed with or without cell selection or depletion, and to evaluate the sequelae of such contamination. STUDY DESIGN AND METHODS:Bacterial co...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1996.36996420753.x
更新日期:1996-09-01 00:00:00
abstract:BACKGROUND:The inventory size for cord blood (CB) depends on the ethnic diversity of human leukocyte antigen (HLA) and the size estimation is important for public health in each ethnicity. STUDY DESIGN AND METHODS:We estimate the CB inventory size in Koreans with stored CB units (CBUs) and patients who underwent allog...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12284
更新日期:2014-03-01 00:00:00
abstract:BACKGROUND:The Verax PGD rapid test for bacteria in platelets (PLTs) has been updated to simplify workflow and improve specificity and sensitivity by employing a novel sequential format. The performance of this updated version, called PGDprime, was evaluated to determine its suitability for use as an FDA-cleared "safet...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.16000
更新日期:2020-11-01 00:00:00
abstract:BACKGROUND:In transfusion medicine, frozen red blood cells (RBCs) are an alternative for liquid-stored RBCs. Little is known about the rheologic properties (i.e., aggregability and deformability) of thawed RBCs. In this study the rheologic properties of high-glycerol frozen RBCs and postthaw stored in saline-adenine-gl...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2010.02730.x
更新日期:2010-11-01 00:00:00
abstract:BACKGROUND:To minimize the bacterial contamination rate in blood collected from donors, a study was designed to evaluate the suitability of a single-use chlorhexidine-alcohol antiseptic for donor arm preparation at all blood collection venues in Australia. STUDY DESIGN AND METHODS:A prospective study of bacterial load...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2004.03362.x
更新日期:2004-08-01 00:00:00
abstract:BACKGROUND:Although a recent study reported shorter survivals in human immunodeficiency virus type 1 (HIV-1)-infected patients who received transfusions, no study has analyzed the relationship in such patients between the frequency of opportunistic infection and transfusion. STUDY DESIGN AND METHODS:Records of 196 HIV...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1994.34194098603.x
更新日期:1994-01-01 00:00:00
abstract::Tunneled central venous catheters with ports are increasingly used for therapeutic apheresis procedures. Vortex ports have been used as access for therapeutic apheresis procedures, but are not ideal for therapeutic plasma exchange (TPE) procedures due to lower flow rates. We performed an in vitro experiment to compare...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14494
更新日期:2018-02-01 00:00:00
abstract::The use of the direct manual hexadimethrine bromide (Polybrene) test (DPT) in the investigation of patients for autoimmune hemolytic anemia (AIHA) was evaluated. Seventy-nine blood samples from 68 patients were tested. A direct antiglobulin test (DAT) using monospecific reagents and the DPT were performed, and a conce...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1990.30991048787.x
更新日期:1990-11-01 00:00:00
abstract:BACKGROUND:An optimal cooling rate is critical for cryopreserving cord blood stem cells, so it is generally achieved by using controlled-rate freezers (CRFs). The cooling rate can be interrupted by many factors during cooling; therefore, a proper response to the interruption in cryopreservation is crucial to prevent th...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12774
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:The risk of transfusion reactions (TR) and the cost of blood has led to efforts to reduce blood use. We changed our practice to transfuse just one instead of two units of red blood cells (RBC) when hemoglobin ≤8 g/dL due to patient blood management (PBM) recommendations. METHODS AND MATERIALS:We compared RB...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15285
更新日期:2019-07-01 00:00:00
abstract::Three examples of an antibody defining a 'new' high frequency red blood cell antigen, Era, are described. Family studies show Era to be inherited, but the mode of inheritance may not be straightforward. ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1982.22382224938.x
更新日期:1982-05-01 00:00:00
abstract:BACKGROUND:Human natural killer (NK) cells are thought to play a role in antiviral response and tumor immune surveillance. The molecular mechanisms of down regulation of NK-cell activity observed after red blood cell (RBC) transfusion is still undefined. STUDY DESIGN AND METHODS:Both effects of blood transfusion (ex v...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2010.03000.x
更新日期:2011-07-01 00:00:00
abstract::The morphology of granulocytes collected by continuous-flow centrifugation (CFC), discontinuous-flow centrifugation (DFC), and continuous-flow filtration (CFF) was investigated in 18 healthy donors by means of light microscopy and transmission electron microscopy. Light microscopy study of semithin sections of granulo...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1981.21582040813.x
更新日期:1981-09-01 00:00:00
abstract:BACKGROUND:Partial Rh antigens have been widely described in black individuals. Carriers are prone to immunization when exposed to the normal antigens. In sickle cell disease (SCD), patient alloimmunization is a major cause of transfusion failure. The potential of individuals with partial C antigen to make anti-C has n...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2009.02382.x
更新日期:2010-01-01 00:00:00
abstract::Anti-Ytb was readily eluted from the cord red blood cells of the second child of a mother with both anti-Ytb and anti-Kell in her serum. The baby was Yt (a+b+) and Kell negative. The cord red blood cells were found to have a weakly positive direct antiglobulin test. Clinically evident hemolytic disease of the newborn ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1979.19580059814.x
更新日期:1979-09-01 00:00:00
abstract:BACKGROUND:Acetaminophen and diphenhydramine are commonly used as pretransfusion medications to prevent transfusion reactions. The purpose of this study was to prospectively compare the risk of transfusion reactions in hematology/oncology patients who receive acetaminophen with diphenhydramine or placebo before transfu...
journal_title:Transfusion
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1537-2995.2008.01858.x
更新日期:2008-11-01 00:00:00
abstract:BACKGROUND:The FDA requirement for sensitivity of viral NAT methods used in blood screening is a 95-percent detection limit of 100 copies per mL, whereas the NAT screening system should have a sensitivity of at least 5000 copies per mL per individual donation. According to the Common Technical Specifications of the Eur...
journal_title:Transfusion
pub_type: 杂志文章,多中心研究
doi:10.1046/j.1537-2995.2002.00101.x
更新日期:2002-05-01 00:00:00
abstract:BACKGROUND:Blood components are often given prophylactically before the placement of invasive lines in patients with coagulation defects. Little, however, is known about the epidemiology of defects in these patients. The purpose of this study is to ascertain what proportion of intensive care patients who receive invasi...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1996.36996420763.x
更新日期:1996-09-01 00:00:00
abstract:BACKGROUND:Many transfusion services determine the titer of potentially incompatible plasma-containing products by performing a one-dilution titer at their selected titer threshold. This study compared the results of immediate spin (IS) one-dilution titers determined by three methods with a reference standard method. ...
journal_title:Transfusion
pub_type: 杂志文章,多中心研究
doi:10.1111/trf.15170
更新日期:2019-04-01 00:00:00
abstract:BACKGROUND:Identification of antibodies against high-prevalence Scianna (Sc; ERMAP) antigens, like Sc1 and Sc5, is difficult and may incur delays in blood procurement and costs. The detection of additional clinically significant alloantibodies is hampered in the presence of anti-Scianna. Soluble recombinant Scianna pro...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2009.02255.x
更新日期:2009-10-01 00:00:00
abstract::Anti-D sensitized red cells were converted to stroma, either by digitonin or hypotonic lysis, and eluates prepared by the modified rapid acid-stromal method. SDS-PAGE and both rocket- and crossed-immunoelectrophoresis showed that, of the total protein content of the eluates, 11 to 25 percent was IgG, 18 to 45 percent ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1983.23383224906.x
更新日期:1983-05-01 00:00:00
abstract:BACKGROUND:Red blood cell (RBC) product wastage in hospitals is reported to range from 0.1% to 6.7%. Wastage at our institution averaged 4.4% of 63,000 issued RBC products. Data indicated that approximately 87% of wasted RBC units were either individual units that were out of blood bank for more than 30 minutes (dispen...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2010.02679.x
更新日期:2010-09-01 00:00:00
abstract:BACKGROUND:Low-titer group O whole blood (LTOWB) is increasingly being used in the civilian trauma setting, although there is a risk of hemolysis. This study evaluated the impact on hemolytic markers following the transfusion of 4 or more units of uncrossmatched LTOWB. METHODS:Civilian adult trauma patients who receiv...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15629
更新日期:2020-06-01 00:00:00
abstract:BACKGROUND:Conventional pretransfusion testing uses hemagglutination to ensure donor-recipient compatibility for ABO/D status and recipient alloantibodies. While screening large numbers of donor units for multiple antigens by hemagglutination is impractical, novel methods of DNA analysis permit the rapid determination ...
journal_title:Transfusion
pub_type: 杂志文章,多中心研究
doi:10.1111/j.1537-2995.2009.02462.x
更新日期:2010-03-01 00:00:00
abstract::Transfusion medicine (TM) physicians provide medical services that benefit all patients such as providing 24-hour laboratory coverage, advising health care providers on test interpretation and selection, validating new methods, and supervising technical personnel. These services ensure delivery of accurate, reliable, ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14264
更新日期:2017-10-01 00:00:00
abstract::The ability to store platelets beyond 24 hours requires a functionally closed system. This study tested the ability of a cell separator bowl seal system to resist penetration of microbial contamination under normal running conditions and under extreme environmental stress. Three test organisms, Micrococcus luteus, Ser...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1990.30290162901.x
更新日期:1990-02-01 00:00:00
abstract::Although the high (40 to 50 per cent) glycerol method of freezing red blood cells has many advantages, no feasible procedure for deglycerolizing with only a clinical centrifuge has been available. Thus this method has been restricted to blood centers that distribute a sufficient number of frozen cells to warrant the i...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1977.17578014580.x
更新日期:1977-09-01 00:00:00
abstract:BACKGROUND:Tissue hypoxia and reperfusion induce abnormal hemostatic function. Therefore, bleeding after total knee replacement (TKR) may be a result of a tourniquet-induced imbalance of the procoagulant and fibrinolytic systems. Because laboratory confirmation of tourniquet-induced abnormal hemostasis is difficult to ...
journal_title:Transfusion
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1046/j.1537-2995.2001.41101285.x
更新日期:2001-10-01 00:00:00
abstract::Platelet concentrates were prepared by plateletpheresis using discontinuous flow centrifugation. Platelet units were stored in PL-146 bags of 300 ml and 2,000 ml capacity, and in vitro measures helpful in predicting platelet viability were compared. Storage in bags of 300 ml capacity led to significant fall in pH, dec...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1978.18278160588.x
更新日期:1978-03-01 00:00:00
abstract::The authors describe a prototype elution method employing chloroquine, a quinoline derivative, to elute IgG antibodies from the platelet surface. This chloroquine elution technique is relatively easy to perform and is effective in the removal of alloantibodies from the platelet surface. Eluted alloantibody was immunol...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1988.28288179016.x
更新日期:1988-03-01 00:00:00