Abstract:
BACKGROUND:It is important to compare the incidence of bacterial contamination of components collected from the peripheral blood or bone marrow (BM), as well as of components processed with or without cell selection or depletion, and to evaluate the sequelae of such contamination. STUDY DESIGN AND METHODS:Bacterial contamination rates were compared in 1380 untreated autologous peripheral blood progenitor cells (PBPCs), 291 untreated autologous BM samples, 916 monoclonal antibody (MoAb)-treated autologous and allogeneic BM samples, and in 45 autologous PBPC components from which the CD34+ cells were selected. Bacterial cultures were performed at sequential time points during the processing of MoAb-treated BM. RESULTS:Bacterial contamination was documented in 44 of 2632 components from 1593 patients (1.67% of components, 2.76% of patients) before cryopreservation. Although only 0.65% of untreated PBPCs were contaminated before cryopreservation, each patient was more likely to have given a contaminated PBPC component than a contaminated BM component (2.41% vs. 0%, p < 0.01). Bacterial contamination of MoAb-treated BM was greater during or after manipulation than it was before (2.33% vs. 0.77%, p < 0.05). At thawing, contamination was documented in 42 (1.97%) of 2136 components cultured. Ten (13.7%) of 73 patients who received hematopoietic progenitor cells that were contaminated before cryopreservation or at thawing developed fever or positive blood cultures within 48 hours of transfusion. Fever was associated with bacteremia in two cases, but no irreversible clinical sequelae were noted. CONCLUSION:These studies suggest that, despite careful attention to sterile procedures, low-level contamination of hematopoietic stem cell components can be introduced before or during manipulation as well as at thawing, and that standards for monitoring of the procedures for collection, processing, cryopreservation, thawing, and transfusion of hematopoietic progenitor cells are necessary.
journal_name
Transfusionjournal_title
Transfusionauthors
Webb IJ,Coral FS,Andersen JW,Elias AD,Finberg RW,Nadler LM,Ritz J,Anderson KCdoi
10.1046/j.1537-2995.1996.36996420753.xsubject
Has Abstractpub_date
1996-09-01 00:00:00pages
782-8issue
9eissn
0041-1132issn
1537-2995journal_volume
36pub_type
杂志文章相关文献
TRANSFUSION文献大全abstract:BACKGROUND:Transfusion-associated microchimerism (TA-MC), the persistence of significant levels of donor white blood cells (WBCs) in blood recipients for prolonged periods, has been demonstrated after nonleukoreduced and leukoreduced transfusion to patients with severe traumatic injury. Development of TA-MC has not bee...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2011.03366.x
更新日期:2012-05-01 00:00:00
abstract::After immunization with native human red cells and red cells infected with Plasmodium falciparum, mouse splenocytes were fused with a myeloma cell line to obtain hybridomas. Among the antibodies specific for blood group antigens, four antibodies (iB3C4, F12, MR432, and iB4) directed against epitopes related to the Rh ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1988.28188127953.x
更新日期:1988-01-01 00:00:00
abstract:BACKGROUND:Obstetric services depend on the transfusion service (TS) to provide diagnostic testing and blood component therapy for clinical care pathways. STUDY DESIGN AND METHODS:We describe three quality improvement (QI) initiatives implemented to improve TS support of obstetric services. RESULTS:We implemented a p...
journal_title:Transfusion
pub_type: 杂志文章,评审
doi:10.1111/j.1537-2995.2011.03152.x
更新日期:2011-12-01 00:00:00
abstract:BACKGROUND:The direct transfusion of thawed hematopoietic progenitor cells (HPCs) is associated to transfusion-related side effects that are thought to be dose-dependent on the infused dimethyl sulfoxide (DMSO). Both the effectiveness of a fully automated cell processing device to washing out DMSO and the effects of DM...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2005.00213.x
更新日期:2005-08-01 00:00:00
abstract::Red blood cells stored in saline solution for two weeks at 12 C lose some of their Fya, Fyb, and Fy3 antigenicity. At the same time substances with specific inhibitory activity against appropriate Duffy antibodies appear in the saline. Similar changes could not be recognized in time-expired commercial reagent red bloo...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1981.21381201813.x
更新日期:1981-05-01 00:00:00
abstract:BACKGROUND:Accurate and reliable measurement of the volume of fetal D+ cells in D- women is required for adequate anti-D prophylaxis. A semiautomated flow cytometry assay based on a standardized calibration curve that was created with simulated fetomatemal hemorrhage (FMH) mixtures was developed. STUDY DESIGN AND METH...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2002.00159.x
更新日期:2002-08-01 00:00:00
abstract::The development of flexible plastic blood bags has permitted effective blood component production and therapy. However, the plasticizer di(2-ethylhexyl)phthalate (DEHP), whose toxicity in humans is still undefined, is known to leach from the plastic into stored blood. Despite the availability of bags made of plastics ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1984.24685066808.x
更新日期:1984-11-01 00:00:00
abstract:BACKGROUND:The risk of transferring blood-borne infections during transfusion is continually increasing because of newly emerging and reemerging viruses. Development of a rapid screening method for emerging viruses that might be transmitted by transfusion is required to eliminate such pathogens during blood donor scree...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12193
更新日期:2013-10-01 00:00:00
abstract:BACKGROUND:Screening of blood donors for antibody to human immunodeficiency virus Types 1 and 2 (anti-HIV-1/2) and/or HIV nucleic acid test (NAT) is a well-established venue to prevent HIV transfusion-transmitted disease. However, with the current available technologies, HIV testing may result in donor loss due to fals...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.13282
更新日期:2016-01-01 00:00:00
abstract::Donation willingness is generally high but registering with donor centers lags behind. We need to understand why and when during the life course which groups of individuals are motivated to donate. With this knowledge, we can design targeted recruitment and retention strategies and improve evidence-based donor managem...
journal_title:Transfusion
pub_type: 杂志文章,评审
doi:10.1111/trf.15268
更新日期:2019-06-01 00:00:00
abstract:BACKGROUND:The greatest limiting factor in the use of umbilical cord blood (UCB) for transplantations is the number of stem cells available in any given collected unit. This study examines maternal, neonatal, and obstetric factors that influence the suitability for banking and transplantation of UCB units collected in ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12340
更新日期:2014-03-01 00:00:00
abstract:BACKGROUND:Blood transfusion is the first-line treatment for patients with thalassemia and many sickle cell patients. However, cases of unregulated blood transfusion are shown to carry a high risk of alloimmunization to red blood cells (RBCs), which can lead to a hemolytic transfusion reaction and be fatal to patients....
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15682
更新日期:2020-02-01 00:00:00
abstract:BACKGROUND:A previous study showed that white cells in blood units undergo apoptosis during storage. STUDY DESIGN AND METHODS:The present study attempts to show the release of nuclear matrix protein (NMP) in the supernatants of red cell units and to determine whether antibodies against nuclear components may be presen...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2000.40020169.x
更新日期:2000-02-01 00:00:00
abstract::The purpose of this investigation was to determine the influence of granulocyte count on the yield of granulocytes. Healthy adult volunteer donors underwent filtration leukapheresis without premedication. The granulocyte count was measured before and immediately after the procedure. In 73 donors, there was an increase...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1979.19479250187.x
更新日期:1979-07-01 00:00:00
abstract:BACKGROUND:Hemolysis due to D incompatibility in the setting of liver transplantation is less frequent than that associated with ABO incompatibility, but can represent an equally adverse event. Approximately 10 percent of ABO-compatible liver transplants involve a D- donor and a D+ recipient. CASE REPORT:A case of sev...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2004.03382.x
更新日期:2004-11-01 00:00:00
abstract:BACKGROUND:The capability of holding whole blood (WB) units at ambient temperature, overnight, should help in platelet (PLT) concentrate preparation logistics. We summarize the results of a study conducted in the early 1990s that compared, in particular, PLT and red blood cell (RBC) in vivo viability properties followi...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2010.02958.x
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:Antibodies to Mia , MUT, and Mur are among the most frequently identified alloantibodies in Southeast Asia. Understanding the characteristics of these antibodies in terms of induction and evanescence would aid in optimizing methods for their detection. STUDY DESIGN AND METHODS:Antibody testing results betwe...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14538
更新日期:2018-05-01 00:00:00
abstract:BACKGROUND:Selection of CD34+ PBPCs has been applied as a method of reducing graft contamination from neoplastic cells. This procedure seems to delay lymphocyte recovery, while myeloid engraftment is no different from that with unselected PBPC transplants. STUDY DESIGN AND METHODS:Lymphocyte recovery was studied in tw...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2001.41060783.x
更新日期:2001-06-01 00:00:00
abstract:BACKGROUND:The proposal to assess the viability capabilities of platelets (PLTs) collected, treated, or stored in a developmental system against "fresh" PLTs from the same subject poses several important methodologic issues pertaining to the timing and manner of the collecting and separating the fresh PLTs. This study ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2005.00177.x
更新日期:2005-07-01 00:00:00
abstract:BACKGROUND:Transfusion-related acute lung injury (TRALI) is an uncommon but serious transfusion reaction. Studies have shown that the transfusion of HLA and HNA antibodies in donor plasma can lead to TRALI. Female donors are more likely to have such antibodies due to alloantigen exposure during pregnancy. Many blood su...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2011.03403.x
更新日期:2012-05-01 00:00:00
abstract:BACKGROUND:The 1000 Genomes Project provides a database of genomic variants from whole genome sequencing of 2504 individuals across five continental superpopulations. This database can enrich our background knowledge of worldwide blood group variant geographic distribution and identify novel variants of potential clini...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14953
更新日期:2018-11-01 00:00:00
abstract:BACKGROUND:Platelet (PLT) storage at 0 to 4 degrees C suppresses bacterial multiplication, but induces clusters of glycoprotein (GP) Ibalpha that trigger their phagocytosis by macrophages and reduce their survival after transfusion. A method was sought that detects cold-induced changes in GPIbalpha involved in phagocyt...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2006.01034.x
更新日期:2006-12-01 00:00:00
abstract::Twenty-five leukaphereses were done by the Haemonetics system using citrated Dextran 75 in normal saline as anticoagulant, and were compared with a much larger series using hydroxyethyl starch (HES) with and without steroid premedication. The granulocyte and platelet yields were only minimally different between the tw...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1977.17277151921.x
更新日期:1977-03-01 00:00:00
abstract:BACKGROUND:Surgical patients receive platelet concentrates (PCs) for a variety of indications. However, there is limited evidence for efficacy or dosing of PCs. STUDY DESIGN AND METHODS:We performed a retrospective cohort study of surgical patients receiving isolated PC transfusion at a single academic tertiary medica...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15156
更新日期:2019-05-01 00:00:00
abstract:BACKGROUND:Biological and technical variability has been increasingly appreciated as a key factor impacting red blood cell (RBC) storability and, potentially, transfusion outcomes. Here, we performed metabolomics analyses to investigate the impact of factors other than storage duration on the metabolic phenotypes of st...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14979
更新日期:2019-01-01 00:00:00
abstract:BACKGROUND:Antibodies specific for the neutrophil antigen HNA-3a cause severe, sometimes fatal transfusion-related acute lung disease (TRALI) when transfused, but it has not been possible to screen blood donors for anti-HNA-3a because using neutrophils as targets was impractical and molecular properties of the antigen ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2011.03145.x
更新日期:2011-10-01 00:00:00
abstract:BACKGROUND:Blood donations collected at the National Blood Center, the Thai Red Cross Society, Bangkok, in 2007 were tested by nucleic acid amplification technology (NAT) using the Chiron TIGRIS/Procleix Ultrio test and the Roche cobas s 201/cobas TaqScreen multiplex (MPX) test. STUDY DESIGN AND METHODS:The sensitivit...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2009.02176.x
更新日期:2009-06-01 00:00:00
abstract:BACKGROUND:Although P1 and Xga are known to be associated with the A4GALT and XG genes, respectively, the genetic basis of antigen expression has been elusive. Recent reports link both P1 and Xga expression with nucleotide changes in the promotor regions and with antigen-negative phenotypes due to disruption of transcr...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15089
更新日期:2019-03-01 00:00:00
abstract:BACKGROUND:Most patients with severe aplastic anemia (sAA) respond to immunosuppression, but a significant number relapse or develop clonal abnormalities such as paroxysmal nocturnal hemoglobinuria, myelodysplasia, or leukemia. In principle, patients without matched sibling donors and older patients might benefit from ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2007.01258.x
更新日期:2007-07-01 00:00:00
abstract:BACKGROUND:In experimental pneumonia we found that transfused older blood increased mortality and lung injury that was associated with increased in vivo hemolysis and elevated plasma cell-free hemoglobin (CFH), non-transferrin-bound iron (NTBI), and plasma labile iron (PLI) levels. In this study, we additionally analyz...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12607
更新日期:2014-07-01 00:00:00