Abstract:
BACKGROUND:Biological and technical variability has been increasingly appreciated as a key factor impacting red blood cell (RBC) storability and, potentially, transfusion outcomes. Here, we performed metabolomics analyses to investigate the impact of factors other than storage duration on the metabolic phenotypes of stored RBC in a multicenter study. STUDY DESIGN AND METHODS:Within the framework of the REDS-III (Recipient Epidemiology and Donor Evaluation Study-III) RBC-Omics study, 13,403 donors were enrolled from four blood centers across the United States and tested for the propensity of their RBCs to hemolyze after 42 days of storage. Extreme hemolyzers were recalled and donated a second unit of blood. Units were stored for 10, 23, and 42 days prior to sample acquisition for metabolomics analyses. RESULTS:Unsupervised analyses of metabolomics data from 599 selected samples revealed a strong impact (14.2% of variance) of storage duration on metabolic phenotypes of RBCs. The blood center collecting and processing the units explained an additional 12.2% of the total variance, a difference primarily attributable to the storage additive (additive solution 1 vs. additive solution 3) used in the different hubs. Samples stored in mannitol-free/citrate-loaded AS-3 were characterized by elevated levels of high-energy compounds, improved glycolysis, and glutathione homeostasis. Increased methionine metabolism and activation of the transsulfuration pathway was noted in samples processed in the center using additive solution 1. CONCLUSION:Blood processing impacts the metabolic heterogeneity of stored RBCs from the largest multicenter metabolomics study in transfusion medicine to date. Studies are needed to understand if these metabolic differences influenced by processing/storage strategies impact the effectiveness of transfusions clinically.
journal_name
Transfusionjournal_title
Transfusionauthors
D'Alessandro A,Culp-Hill R,Reisz JA,Anderson M,Fu X,Nemkov T,Gehrke S,Zheng C,Kanias T,Guo Y,Page G,Gladwin MT,Kleinman S,Lanteri M,Stone M,Busch M,Zimring JC,Recipient Epidemiology and Donor Evaluation Study-III (REDS-doi
10.1111/trf.14979subject
Has Abstractpub_date
2019-01-01 00:00:00pages
89-100issue
1eissn
0041-1132issn
1537-2995journal_volume
59pub_type
杂志文章相关文献
TRANSFUSION文献大全abstract:BACKGROUND:This study compared the likelihood of return to donate and donation rate ratio by age of donors at their first donation when followed up to 12 years. STUDY DESIGN AND METHODS:Donation history of two cohorts of first-time donors (those donating in 2007 and 2013) was extracted until March 2019 from Australian...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.16187
更新日期:2020-11-12 00:00:00
abstract:BACKGROUND:Intracellular glutathione declines progressively in platelet concentrates stored for transfusion, but little is known about the nature of this process. STUDY DESIGN AND METHODS:Acivicin, an irreversible inhibitor of gamma-glutamyl transpeptidase, was used to determine the fate of glutathione lost from plate...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1994.34594249055.x
更新日期:1994-05-01 00:00:00
abstract::Donor deferral rates in regional blood centers vary from 5 to 24 per cent, reducing by more than 1,250,000 the number of units of volunteer blood available for transfusion in the nation each year. Those criteria for donor deferral which are intended to exclude donors likely to suffer a "donor reaction" are based parti...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1980.20581034503.x
更新日期:1980-09-01 00:00:00
abstract:BACKGROUND:Our blood bank is part of a large academic institution with an active sickle cell anemia program. We provide sickle patients with blood phenotypically matched for C/c, E/e, and K antigens. Since licensed reagents are available for phenotyping C/c, E/e, and K on an automated blood analyzer, we decided to eval...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12270
更新日期:2014-02-01 00:00:00
abstract:BACKGROUND:Iron supplements reduce the risk of iron depletion in blood donors. Recent initiatives have sought to increase use of donor iron supplementation (IS), but a baseline description of blood donor supplement practices is needed. STUDY DESIGN AND METHODS:Presenting blood donors received a questionnaire inquiring...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15407
更新日期:2019-09-01 00:00:00
abstract:BACKGROUND:Transfusion-associated microchimerism (TA-MC), the persistence of significant levels of donor white blood cells (WBCs) in blood recipients for prolonged periods, has been demonstrated after nonleukoreduced and leukoreduced transfusion to patients with severe traumatic injury. Development of TA-MC has not bee...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2011.03366.x
更新日期:2012-05-01 00:00:00
abstract::Soft and hard-packed red blood cells in four different CPD anticoagulant-preservative solutions were stored with and without added glucose, adenine, and electrolytes. The hemoglobin-oxygen affinity of the red blood cell concentrates was tested over a six-week storage period. No single solution conferred better protect...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1981.21682085761.x
更新日期:1981-11-01 00:00:00
abstract:BACKGROUND:The Verax PGD rapid test for bacteria in platelets (PLTs) has been updated to simplify workflow and improve specificity and sensitivity by employing a novel sequential format. The performance of this updated version, called PGDprime, was evaluated to determine its suitability for use as an FDA-cleared "safet...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.16000
更新日期:2020-11-01 00:00:00
abstract:BACKGROUND:Posttransfusion graft-versus-host disease (PT-GVHD) is underdiagnosed and underreported. Risk factors predisposing to PT-GVHD in newborn recipients, the clinical manifestations of the disease in newborns, and its mechanism are not well characterized. STUDY DESIGN AND METHODS:A literature review identified 2...
journal_title:Transfusion
pub_type: 杂志文章,评审
doi:10.1046/j.1537-2995.1996.36296181922.x
更新日期:1996-02-01 00:00:00
abstract:BACKGROUND:Hemolysis of red blood cells (RBCs) during blood bank storage is the most obvious manifestation of RBC storage system failure. However, its analysis is made difficult because the largest source of interunit difference is donor specific. Availability of data from national blood systems on large numbers of RBC...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2009.02275.x
更新日期:2009-12-01 00:00:00
abstract::Pyruvate was placed in experimental CPD-adenine (0.25 mM) blood preservative mixtures in four concentrations ranging from 40 to 320 mM. In the 320 mM pyruvate preservative, 2,3-DPG levels were elevated above normal for six weeks of whole blood storage at 4 C. The lower pyruvate concentrations maintained elevated or no...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1980.20280169965.x
更新日期:1980-03-01 00:00:00
abstract:BACKGROUND:The AABB Clinical Transfusion Medicine Committee (CTMC) compiles an annual synopsis of the published literature covering important developments in the field of transfusion medicine (TM) for the board of director's review. This synopsis is now made available as a manuscript published in TRANSFUSION. STUDY DE...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15848
更新日期:2020-07-01 00:00:00
abstract:BACKGROUND:Transfusion of plasma is a basic treatment for complex coagulopathies as well as in major blood loss. Early transfusion of plasma after trauma with major hemorrhage has been recommended by retrospective studies. However, the use of plasma is often hampered by the need to maintain a cold chain and the time ne...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12191
更新日期:2013-12-01 00:00:00
abstract:BACKGROUND:The overall number of red blood cell (RBC) units distributed to hospitals throughout the world and in the United States has decreased lately. This study was performed to determine if the number of antigen-negative RBC units distributed to hospitals has followed this trend. STUDY DESIGN AND METHODS:Stratifie...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14376
更新日期:2018-01-01 00:00:00
abstract::HLA and granulocyte-specific antibodies have been implicated in the production of transfusion-related acute lung injury (TRALI). Reported here is a case that suggests that the patient's preexisting condition may play an important role in determining whether TRALI develops upon transfusion of blood products containing ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1990.30190117629.x
更新日期:1990-01-01 00:00:00
abstract::We describe a nonanaphylactic hemoglobinuric reaction caused by transfusion of frozen red cells, deglycerolyzed by standard technique, obtained from a donor with undiagnosed sickle trait. Although significant hemoglobinemia and hemoglobinuria were noted, the recipient experienced no adverse clinical reactions. The ben...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1984.24284173352.x
更新日期:1984-03-01 00:00:00
abstract:BACKGROUND:Hypoxia is a major cause of painful vaso-occlusive crisis in sickle cell disease (SCD). Simple transfusion and red blood cell (RBC) exchange are commonly used as preventive therapies whose aim is to dilute hemoglobin (Hb)S-containing RBCs (SS-RBCs) with normal RBCs (AA-RBCs) to prevent sickling. We hypothesi...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2010.02663.x
更新日期:2010-10-01 00:00:00
abstract:BACKGROUND:Our study characterizes blood use in the ambulatory setting by US elderly Medicare beneficiaries during 2001. As the US population ages and delivery of health care in outpatient settings is on the rise, ambulatory blood utilization is expected to increase. There is currently a lack of broad population-based ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2009.02114.x
更新日期:2009-06-01 00:00:00
abstract::ALX148, a novel CD47 blocking agent, is in clinical development for the treatment of advanced solid tumors and lymphoma. Because CD47 is highly expressed on red blood cells (RBCs), its therapeutic blockade can potentially interfere with pretransfusion compatibility testing. This study describes the interference of ALX...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.16009
更新日期:2020-08-20 00:00:00
abstract::Forty samples of frozen human plasma were thawed in a 37 C water bath following storage at -80 C for 48 hours. Twenty of these samples were protected by heat-sealed polyethylene overwraps prior to freezing and thawing. The remaining 20 samples were unprotected. None of the protected samples leaked during thawing. The ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1981.21481276002.x
更新日期:1981-07-01 00:00:00
abstract:BACKGROUND:Commercially available additive solutions (ASs) require 30% to 35% plasma for optimal storage of platelets (PLTs). PLTs suspended in M-sol, a bicarbonate-based experimental platelet additive solution (PAS), maintain in vitro PLT properties during storage with low levels of plasma (< or =5%). STUDY DESIGN AN...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2009.02539.x
更新日期:2010-05-01 00:00:00
abstract::Leukapheresis by continuous flow centrifugation (CFC) or filtration (FL) were compared in untreated and corticosteroid-treated donors. The administration of prednisone 60 mg orally 10 to 12 hours before leukapheresis increased significantly both the donor's prepheresis WBC counts and the total granulocyte yields by CF...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1976.16376225492.x
更新日期:1976-05-01 00:00:00
abstract::A microwave oven has been specifically modified to permit rapid thawing of fresh-frozen plasma (FFP) by using a rotating disc with a temperature sensor to hold the plasma bag. This modification makes it possible to mix the FFP continuously during thawing, and automatically shuts the oven off when the plasma reaches 21...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1984.24184122564.x
更新日期:1984-01-01 00:00:00
abstract:BACKGROUND:When problems with compatibility beyond ABO and D arise, currently transfusion services search their inventories and perform time-consuming serologic testing to locate antigen-negative blood. These clinically important blood group antigens can be detected reliably by red cell genotyping, which is a technolog...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.13168
更新日期:2015-11-01 00:00:00
abstract:BACKGROUND:Accurate and reliable measurement of the volume of fetal D+ cells in D- women is required for adequate anti-D prophylaxis. A semiautomated flow cytometry assay based on a standardized calibration curve that was created with simulated fetomatemal hemorrhage (FMH) mixtures was developed. STUDY DESIGN AND METH...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2002.00159.x
更新日期:2002-08-01 00:00:00
abstract:BACKGROUND:Iron deficiency represents a risk to donor health and the blood supply. Efficacy trials indicate that postdonation iron replacement improves iron stores but they do not account for complexities of implementation in the routine collection context. We therefore conducted two prospective feasibility studies in ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14173
更新日期:2017-08-01 00:00:00
abstract:BACKGROUND:During the use of commercial red cell (RBC) acid-elution kits for adsorption and elution (adsorption/elution) studies with anti-D, unexpected reactive eluates (anti-D) were obtained from D- RBCs. Such results were not obtained with a parallel xylene method or, historically, with heat and ether methods. STUD...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1998.38698326336.x
更新日期:1998-06-01 00:00:00
abstract:BACKGROUND:The FcgammaRIIA gene is expressed in two polymorphic forms, R131 and H131, which differ by the replacement of histidine by arginine at position 131. The FCGR3B (FcgammaRIIIB) gene exists in two allelic isoforms, known as FCGR3B1 (FcgammaRIIIB-NA1) and FCGR3B2 (FcgammaRIIIB-NA2), which differ in nucleotides 1...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2000.40111388.x
更新日期:2000-11-01 00:00:00
abstract:BACKGROUND:Transfusion-related characteristics have been hypothesized to cause allergic transfusion reactions (ATRs) but they have not been thoroughly studied. The primary objective of this study is to evaluate the associations of infusion rate, infusion volume, ABO mismatching, component age, and pretransfusion medica...
journal_title:Transfusion
pub_type: 临床试验,杂志文章
doi:10.1111/trf.12852
更新日期:2015-02-01 00:00:00
abstract:BACKGROUND:In the 20th century, blood transfusion has become an indispensable therapy in carrying out and improving many medical and surgical applications. Its scope of influence goes well beyond that of medicine, because blood donation, an action with high social significance, is completely connected to progress in th...
journal_title:Transfusion
pub_type: 历史文章,杂志文章
doi:10.1111/j.1537-2995.2007.01634.x
更新日期:2008-05-01 00:00:00