False-positive eluate reactivity due to the low-ionic wash solution used with commercial acid-elution kits.

Abstract:

BACKGROUND:During the use of commercial red cell (RBC) acid-elution kits for adsorption and elution (adsorption/elution) studies with anti-D, unexpected reactive eluates (anti-D) were obtained from D- RBCs. Such results were not obtained with a parallel xylene method or, historically, with heat and ether methods. STUDY DESIGN AND METHODS:Single-donor and commercial polyclonal anti-D samples were incubated with D+ and D- RBCs. Acid eluates were prepared by the manufacturers' directions. Variations in the wash step of the eluate preparation included the use of commercial kit wash solution versus phosphate-buffered saline versus solutions of various ionic strengths. RESULTS:Anti-D was eluted from 20 of 22 samples of D- RBCs after incubation with commercial polyclonal anti-D (titer 512) and from 2 of 3 samples of D- RBCs incubated with single-donor anti-D (titer 256). With a low-titer (16) single-donor anti-D, 0 of 4 eluates from D- RBCs reacted. When phosphate-buffered saline was substituted for the commercial wash solution, 0 of 11 D- RBC eluates reacted, as compared with 9 of 11 D- RBCs that yielded positive 1+(-)2+ eluates with the commercial wash solution. If the recommended initial phosphate-buffered saline wash was omitted before the use of the commercial wash solution, the eluate reactivity was stronger (2+(-)3+). When low-ionic-strength (< 0.03 M) saline was substituted, anti-D was eluted from D- RBCs. All last washes were nonreactive. Antiglobulin tests on all adsorbing D- were negative. CONCLUSION:Commercial wash solutions used for acid elution are at low ionic strength and commonly yield superior eluates, but in the presence of high-titer antibodies, false-positive eluates can result. It is our belief that the low-ionic-strength wash solution caused aggregation of IgG and nonspecific attachment of IgG on RBCs. Aggregates will contain IgG serum antibodies in proportion to the titer of the antibody. It is this nonspecifically bound antibody that is eluted from antigen-negative RBCs.

journal_name

Transfusion

journal_title

Transfusion

authors

Leger RM,Arndt PA,Ciesielski DJ,Garratty G

doi

10.1046/j.1537-2995.1998.38698326336.x

subject

Has Abstract

pub_date

1998-06-01 00:00:00

pages

565-72

issue

6

eissn

0041-1132

issn

1537-2995

journal_volume

38

pub_type

杂志文章
  • Influence of enteral feeding and anemia on tissue oxygen extraction after red blood cell transfusion in preterm infants.

    abstract:BACKGROUND:Understanding factors that impact tissue oxygen extraction may guide red blood cell (RBC) transfusion decision making in preterm infants. Our objective was to assess the influence of enteral feeding and anemia on cerebral and mesenteric oxygen saturation (Csat and Msat) and fractional tissue oxygen extractio...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.15680

    authors: Goldstein GP,Rao A,Ling AY,Ding VY,Chang IJ,Chock VY

    更新日期:2020-03-01 00:00:00

  • Glucose-6-phosphate dehydrogenase activity decreases during storage of leukoreduced red blood cells.

    abstract:BACKGROUND:During storage, the activity of the red blood cell (RBC) antioxidant system decreases. Glucose-6-phosphate dehydrogenase (G6PD) is essential for protection against oxidative stress by producing NADPH. G6PD function of RBC transfusion products is reported to remain stable during storage, but activity was meas...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.13378

    authors: Peters AL,van Bruggen R,de Korte D,Van Noorden CJ,Vlaar AP

    更新日期:2016-02-01 00:00:00

  • Absorption of anti-Chido from serum using autologous red blood cells coated with homologous C4.

    abstract::A method is described which absorbs anti-Chido from serum without reducing the strength of other alloantibodies. Autologous red blood cells are coated with homologous C4 by a low-ionic-strength method and then used for the absorption. This is a practical method for removing anti-Chido from serum so that underlying all...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1982.22382224951.x

    authors: Ellisor SS,Shoemaker MM,Reid ME

    更新日期:1982-05-01 00:00:00

  • Effects of new brochures on blood donor recruitment and retention.

    abstract:BACKGROUND:Currently, donors may arrive at blood collection sites without prior knowledge of eligibility and deferral criteria. STUDY DESIGN AND METHODS:The effects of distributing newly developed recruitment brochures 2 weeks in advance of blood drives and the provision of brochures on temporary deferral at the takin...

    journal_title:Transfusion

    pub_type: 临床试验,杂志文章

    doi:10.1046/j.1537-2995.1994.34794330012.x

    authors: Gimble JG,Kline L,Makris N,Muenz LR,Friedman LI

    更新日期:1994-07-01 00:00:00

  • Adverse reactions, psychological factors, and their effect on donor retention in men and women.

    abstract:BACKGROUND:This study investigates the effect of a vasovagal reaction (VVR) or needle reaction (NR) on the risk of stopping as a blood donor, taking into account variables from the Theory of Planned Behavior (TPB). Is stopping risk solely related to the adverse reaction itself, or do the TPB variables play a role as we...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2011.03551.x

    authors: Veldhuizen I,Atsma F,van Dongen A,de Kort W

    更新日期:2012-09-01 00:00:00

  • A multicenter evaluation of reproducibility of swirling in platelet concentrates. Biomedical Excellence for Safer Transfusion (BEST) Working Party of the International Society of Blood Transfusion.

    abstract:BACKGROUND:Eleven laboratories participated in a study investigating whether swirling can be used in monitoring platelet discoid morphology in platelet concentrates (PCs). STUDY DESIGN AND METHODS:In each laboratory, two readers observed the swirling pattern in 1- to 5-day-old PCs, in PCs containing spherical platelet...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1994.34994378282.x

    authors: Bertolini F,Murphy S

    更新日期:1994-09-01 00:00:00

  • Unusual kinetics of white cell clearance in transfused mice.

    abstract:BACKGROUND:Donor white cells (WBCs) in blood transfusions are responsible for complications in recipients, including alloimmunization, graft-versus-host disease (GVHD), and virus transmission and reactivation. The recent use of sequence-specific polymerase chain reaction assays to monitor the kinetics of clearance of d...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1995.35295125737.x

    authors: Goodarzi MO,Lee TH,Pallavicini MG,Donegan EA,Busch MP

    更新日期:1995-02-01 00:00:00

  • Rapid identification of microorganisms from platelet concentrates by matrix-assisted laser desorption ionization time-of-flight mass spectrometry after short-term incubation on liquid medium.

    abstract:BACKGROUND:Platelets (PLTs) are especially affected by the risk of bacterial contamination. Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) is an accurate method for the routine identification of bacterial isolates in microbiology laboratories. We directly applied the MALDI-TOF method to bacteria...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.14430

    authors: Chetouane Y,Dubourg G,Gallian P,Flaudrops C,Chiaroni J,Chabrière E,Raoult D,Camoin-Jau L

    更新日期:2018-03-01 00:00:00

  • Guidelines for blood utilization review.

    abstract::Hospitals are required by accrediting agencies to perform blood utilization review. Specific areas that must be addressed are the ordering, distribution, handling, dispensing, and administration of blood components. Monitoring the effects of transfusion on patients is also required. The format of the review process an...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1994.34594249058.x

    authors: Stehling L,Luban NL,Anderson KC,Sayers MH,Long A,Attar S,Leitman SF,Gould SA,Kruskall MS,Goodnough LT

    更新日期:1994-05-01 00:00:00

  • Phase III randomized study comparing 5 or 10 microg per kg per day of filgrastim for mobilization of peripheral blood progenitor cells with chemotherapy, followed by intensification and autologous transplantation in patients with nonmyeloid malignancies.

    abstract:BACKGROUND:It is not known whether increasing the dose of filgrastim after mobilizing chemotherapy improves collection of peripheral blood progenitor cells (PBPC) and leads to faster hematopoietic engraftment after autologous transplantation. STUDY DESIGN AND METHODS:A randomized, open-label, multicenter trial was car...

    journal_title:Transfusion

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1046/j.1537-2995.2003.00273.x

    authors: André M,Baudoux E,Bron D,Canon JL,D'Hondt V,Fassotte MF,D'Hondt L,Fillet G,Humblet Y,Jerusalem G,Vermeulen P,Symann M,Beguin Y

    更新日期:2003-01-01 00:00:00

  • Absence of transfusion-associated microchimerism in pediatric and adult recipients of leukoreduced and gamma-irradiated blood components.

    abstract:BACKGROUND:Transfusion-associated microchimerism (TA-MC), the persistence of significant levels of donor white blood cells (WBCs) in blood recipients for prolonged periods, has been demonstrated after nonleukoreduced and leukoreduced transfusion to patients with severe traumatic injury. Development of TA-MC has not bee...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2011.03366.x

    authors: Sanchez R,Lee TH,Wen L,Montalvo L,Schechterly C,Colvin C,Alter HJ,Luban NL,Busch MP

    更新日期:2012-05-01 00:00:00

  • Protein quality in Mirasol pathogen reduction technology-treated, apheresis-derived fresh-frozen plasma.

    abstract:BACKGROUND:The Mirasol pathogen reduction technology (PRT) system for plasma is based on a riboflavin (vitamin B(2)) and ultraviolet (UV) light treatment process resulting in pathogen inactivation due to irreversible photo-oxidative damage of nucleic acids. The purpose of this study was to evaluate the in vitro protein...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2009.02517.x

    authors: Smith J,Rock G

    更新日期:2010-04-01 00:00:00

  • First results of a ferritin-based blood donor deferral policy in the Netherlands.

    abstract:BACKGROUND:Whole blood donors are at risk of becoming iron deficient. To monitor iron stores, Sanquin implemented a new deferral policy based on ferritin levels, in addition to the traditional hemoglobin measurements. METHODS:Ferritin levels are determined in every fifth donation, as well as in all first-time donors. ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.15906

    authors: Vinkenoog M,van den Hurk K,van Kraaij M,van Leeuwen M,Janssen MP

    更新日期:2020-08-01 00:00:00

  • The successful implementation of an automated institution-wide assessment of hemoglobin and ABO typing to dynamically estimate red blood cell inventory requirements.

    abstract:BACKGROUND:Blood bank inventories must balance adequate supply with minimal outdate rates. The day-to-day practice of ordering red blood cell (RBC) inventory usually involves manually comparing current inventory levels with predetermined thresholds calculated from historical usage and ordering the difference. To date, ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.15272

    authors: Quinn J,Campbell C,Gomez A,Kumar-Misir A,Watson S,Liwski D,Covello T,Tennankore KK,Chisholm N,Sadek I,Cheng C

    更新日期:2019-07-01 00:00:00

  • A monoclonal antibody with anti-D-like activity in murine immune thrombocytopenia requires Fc domain function for immune thrombocytopenia ameliorative effects.

    abstract:BACKGROUND:The mechanism of action of anti-D in ameliorating immune thrombocytopenia (ITP) remains unclear. The monoclonal antibody (MoAb) Ter119, which targets murine red blood cells (RBCs), has been shown to mimic the effect of anti-D in improving antibody-mediated murine ITP. The mechanism of Ter119-mediated ITP ame...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.13032

    authors: Yu X,Menard M,Seabright G,Crispin M,Lazarus AH

    更新日期:2015-06-01 00:00:00

  • HPC viability measurement: trypan blue versus acridine orange and propidium iodide.

    abstract:BACKGROUND:A reliable, validated method for rapidly determining HPC viability is essential for clinical cell engineering. STUDY DESIGN AND METHODS:A fluorometric cell viability assay using acridine orange and propidium iodide (AO/PI) was compared to the current standard, trypan blue (TB) exclusion. Viable cells staine...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.2000.40060693.x

    authors: Mascotti K,McCullough J,Burger SR

    更新日期:2000-06-01 00:00:00

  • Major transfusions remain frequent despite the generalized use of tranexamic acid: an audit of 3322 patients undergoing cardiac surgery.

    abstract:BACKGROUND:Aprotinin has been reapproved for use in Europe and in Canada. We sought to determine if its reintroduction was still pertinent given the widespread administration of tranexamic acid, another antifibrinolytic shown to reduce bleeding and transfusions. STUDY DESIGN AND METHODS:After institutional review boar...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.13615

    authors: Stevens LM,Noiseux N,Prieto I,Hardy JF

    更新日期:2016-07-01 00:00:00

  • The prevalence of human parvovirus B19 DNA and antibodies in blood donors from four Chinese blood centers.

    abstract:BACKGROUND:Human parvovirus B19 is a common human pathogen that causes a variety of diseases with outcomes ranging from asymptomatic to severe, especially in immunocompromised patients. The B19 virus can be transmitted via blood and/or blood products and its resistance to common viral inactivation and/or removal method...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2011.03067.x

    authors: Ke L,He M,Li C,Liu Y,Gao L,Yao F,Li J,Bi X,Lv Y,Wang J,Hirsch ML,Li W

    更新日期:2011-09-01 00:00:00

  • Serum alanine aminotransferase in blood donors: activity distribution and effect of phenotype.

    abstract::Alanine aminotransferase (ALT) phenotype and serum activities were determined in 200 random volunteer blood donors. Serum enzyme activities were not significantly affected by the ALT phenotype (p greater than 0.1). Studies on 500 random volunteer blood donors showed significant differences in serum ALT levels between ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1982.22482251212.x

    authors: Savage B,Sayers MH,Giblett ER

    更新日期:1982-07-01 00:00:00

  • Determination of thromboelastographic responsiveness in stored single-donor platelet concentrates.

    abstract:BACKGROUND:Thromboelastography (TEG) is widely used in hospitals but less commonly in blood banks for evaluation of platelet (PLT) concentrates (PCs). A TEG-PC assay for testing fresh or stored PLTs must reflect the quality of the PLTs. The added value could be measurement of donor-dependent PLT quality. STUDY DESIGN ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.12515

    authors: Bontekoe IJ,van der Meer PF,de Korte D

    更新日期:2014-06-01 00:00:00

  • Preparation and storage of platelet concentrates.

    abstract::A technique of platelet concentrate preparation and storage is presented which permits the maximum number of viable and functional platelets to be preserved for periods of 72 hours. Although the storage conditions must be followed precisely, the method is nevertheless simple to perform and does not require specialized...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1976.16176130842.x

    authors: Slichter SJ,Harker LA

    更新日期:1976-01-01 00:00:00

  • Development of a quality monitoring program for platelet components: a report of the first four years' experience at Canadian Blood Services.

    abstract:BACKGROUND:A quality monitoring program (QMP) for platelet concentrates (PCs) was implemented at Canadian Blood Services (CBS) to improve standards and to better understand platelet (PLT) products by supplementing routine quality control (QC). STUDY DESIGN AND METHODS:Annual surveys of PCs from CBS production sites we...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2011.03402.x

    authors: Levin E,Jenkins C,Culibrk B,Gyöngyössy-Issa MI,Serrano K,Devine DV

    更新日期:2012-04-01 00:00:00

  • Issues in the quality of umbilical cord blood stem cells for transplantation.

    abstract:BACKGROUND:Because the frequency of umbilical cord blood (UCB) stem cell transplantation has increased, the quality of UCB available in banks is an important part of the success of UCB stem cell transplants. STUDY DESIGN AND METHODS:A quality assurance monitoring system was used to evaluate 268 UCB units provided to u...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2005.04265.x

    authors: McCullough J,McKenna D,Kadidlo D,Schierman T,Wagner J

    更新日期:2005-06-01 00:00:00

  • Effect of blood donation on the establishment of normal ranges of lymphocyte subsets.

    abstract:BACKGROUND:Absolute counts of CD4+ T-lymphocytes are used in the management of patients with human immunodeficiency virus infection. Low absolute counts of CD3+CD4+ cells have also been observed in healthy people--a phenomenon called idiopathic CD4 lymphocytopenia. It is common practice for normal ranges for lymphocyte...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1996.36696269517.x

    authors: Bryant JA,Wylie BR,Yuan FF,Ribeiro A,Thomson AR,Cooley MA,Fletcher A

    更新日期:1996-06-01 00:00:00

  • Low-risk transfusion-related acute lung injury donor strategies and the impact on the onset of transfusion-related acute lung injury: a meta-analysis.

    abstract:BACKGROUND:Transfusion-related acute lung injury (TRALI) is the leading cause of transfusion-related mortality. In the past decade blood banks have implemented low-risk TRALI donor strategies, including a male-only donor policy for plasma-containing blood products to prevent onset of TRALI. We performed a meta-analysis...

    journal_title:Transfusion

    pub_type: 杂志文章,meta分析,评审

    doi:10.1111/trf.12816

    authors: Müller MC,van Stein D,Binnekade JM,van Rhenen DJ,Vlaar AP

    更新日期:2015-01-01 00:00:00

  • The extension of 4 degrees C storage time of frozen-thawed red cells.

    abstract::Blood was drawn from volunteer donors and frozen using the high glycerin, mechanical freezing procedure accepted by the United States Navy. Subsequently, the units of blood were thawed and washed. Various anticoagulants were added, and the red cells were stored in a refrigerator at 4 degrees C for periods of up to 28 ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1992.32492263449.x

    authors: Myhre BA,Marcus CS

    更新日期:1992-05-01 00:00:00

  • Enhanced detection of hepatitis B virus in Hong Kong blood donors after introduction of a more sensitive transcription-mediated amplification assay.

    abstract:BACKGROUND:A total of 517,072 and 399,326 consecutive donations were screened for hepatitis B virus (HBV) by individual-donation nucleic acid testing (ID-NAT) using Ultrio and Ultrio Plus assays (Novartis Diagnostics), respectively. The impact of more sensitive HBV detection by the latter assay version was established ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.12165

    authors: Tsoi WC,Lelie N,Lin CK

    更新日期:2013-10-01 00:00:00

  • Transfusion-acquired hepatitis C: the Danish lookback experience. The Danish HCV [hepatitis C virus] Lookback Group.

    abstract:BACKGROUND:In 1996, the Danish National Board of Health recommended hepatitis C virus (HCV) lookback to identify recipients of blood components from donors found to be positive since the implementation of anti-HCV screening in 1991. STUDY DESIGN AND METHODS:The aim was to accumulate results of the lookback at a nation...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1999.39299154734.x

    authors: Christensen PB,Groenbaek K,Krarup HB

    更新日期:1999-02-01 00:00:00

  • Ultrasound-enabled topical anesthesia for pain reduction of phlebotomy for whole blood donation.

    abstract:BACKGROUND:Ultrasound-facilitated delivery of topical anesthetics has been used to achieve effective anesthesia within 5 minutes for venipuncture and the insertion of intravenous access devices, but has never been studied for blood donation. STUDY DESIGN AND METHODS:This study was a single-center, prospective, randomi...

    journal_title:Transfusion

    pub_type: 杂志文章,随机对照试验

    doi:10.1111/j.1537-2995.2008.01939.x

    authors: Stowell CP,Trieu MQ,Chuang H,Katz N,Quarrington C

    更新日期:2009-01-01 00:00:00

  • A typology of blood donor motivations.

    abstract:BACKGROUND:Although the need for whole blood is declining, so too are the number of first-time and repeat blood donors. To develop new recruitment and retention strategies, therefore, we need to draw on as wide a variation in blood donor motivations as possible. The primary aim of this study is to draw on a large surve...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.15913

    authors: Ferguson E,Hill A,Lam M,Reynolds C,Davison K,Lawrence C,Brailsford SR

    更新日期:2020-09-01 00:00:00