False-positive eluate reactivity due to the low-ionic wash solution used with commercial acid-elution kits.


BACKGROUND:During the use of commercial red cell (RBC) acid-elution kits for adsorption and elution (adsorption/elution) studies with anti-D, unexpected reactive eluates (anti-D) were obtained from D- RBCs. Such results were not obtained with a parallel xylene method or, historically, with heat and ether methods. STUDY DESIGN AND METHODS:Single-donor and commercial polyclonal anti-D samples were incubated with D+ and D- RBCs. Acid eluates were prepared by the manufacturers' directions. Variations in the wash step of the eluate preparation included the use of commercial kit wash solution versus phosphate-buffered saline versus solutions of various ionic strengths. RESULTS:Anti-D was eluted from 20 of 22 samples of D- RBCs after incubation with commercial polyclonal anti-D (titer 512) and from 2 of 3 samples of D- RBCs incubated with single-donor anti-D (titer 256). With a low-titer (16) single-donor anti-D, 0 of 4 eluates from D- RBCs reacted. When phosphate-buffered saline was substituted for the commercial wash solution, 0 of 11 D- RBC eluates reacted, as compared with 9 of 11 D- RBCs that yielded positive 1+(-)2+ eluates with the commercial wash solution. If the recommended initial phosphate-buffered saline wash was omitted before the use of the commercial wash solution, the eluate reactivity was stronger (2+(-)3+). When low-ionic-strength (< 0.03 M) saline was substituted, anti-D was eluted from D- RBCs. All last washes were nonreactive. Antiglobulin tests on all adsorbing D- were negative. CONCLUSION:Commercial wash solutions used for acid elution are at low ionic strength and commonly yield superior eluates, but in the presence of high-titer antibodies, false-positive eluates can result. It is our belief that the low-ionic-strength wash solution caused aggregation of IgG and nonspecific attachment of IgG on RBCs. Aggregates will contain IgG serum antibodies in proportion to the titer of the antibody. It is this nonspecifically bound antibody that is eluted from antigen-negative RBCs.






Leger RM,Arndt PA,Ciesielski DJ,Garratty G




Has Abstract


1998-06-01 00:00:00












  • Risk factors for HCV infection among blood donors confirmed to be positive for the presence of HCV RNA and not reactive for the presence of anti-HCV.

    abstract:BACKGROUND:In 1999, NAT of blood donations was implemented to detect "window-period" infections. Blood donors who have confirmed NAT results positive for the presence of HCV in the absence of anti-HCV are likely to have been recently infected. Of over 26.8 million donations tested between March 3, 1999, and March 31, 2...


    pub_type: 杂志文章


    authors: Orton SL,Stramer SL,Dodd RY,Alter MJ

    更新日期:2004-02-01 00:00:00

  • Prepooled cryoprecipitate for treatment of hemophilia A.

    abstract::Lyophilized factor VIII concentrates, because of their convenience, are the most commonly used products for providing antihemophilic factor to patients with hemophilia A. Heightened concerns about associated complications indicate that cryoprecipitate usage will increase in the immediate future. We present a protocol ...


    pub_type: 杂志文章


    authors: Menitove JE,Spero JA,Richards WA,Lewis JH,Montgomery RR,Gill JC,Hasiba U,Wheeler DV,Sherwin JA,Russell-Vaskov S,Marlar RA,Maxwell NG,Aster RH

    更新日期:1983-05-01 00:00:00

  • Sterility and quality of blood dispensed in syringes for infants.

    abstract::A unit-dose system was designed to dispense precise quantities of blood in a form ready for immediate transfusion into neonatal patients. The principles were similar to those used by pharmacies to dispense individual doses of drugs. In the blood center, the precise volume of blood ordered for a neonatal patient was as...


    pub_type: 杂志文章


    authors: Strauss RG,Crawford GF,Elbert C,Floss AM,Liesch M

    更新日期:1986-03-01 00:00:00

  • A DEL phenotype attributed to RHD Exon 9 sequence deletion: slipped-strand mispairing and blood group polymorphisms.

    abstract:BACKGROUND:The RhD blood group antigen is extremely polymorphic and the DEL phenotype represents one such class of polymorphisms. The DEL phenotype prevalent in East Asian populations arises from a synonymous substitution defined as RHD*1227A. However, initially, based on genomic and cDNA studies, the genetic basis for...


    pub_type: 杂志文章


    authors: Lopez GH,Turner RM,McGowan EC,Schoeman EM,Scott SA,O'Brien H,Millard GM,Roulis EV,Allen AJ,Liew YW,Flower RL,Hyland CA

    更新日期:2018-03-01 00:00:00

  • Down regulation of human natural killer cell-mediated cytolysis induced by blood transfusion: role of transforming growth factor-β(1), soluble Fas ligand, and soluble Class I human leukocyte antigen.

    abstract:BACKGROUND:Human natural killer (NK) cells are thought to play a role in antiviral response and tumor immune surveillance. The molecular mechanisms of down regulation of NK-cell activity observed after red blood cell (RBC) transfusion is still undefined. STUDY DESIGN AND METHODS:Both effects of blood transfusion (ex v...


    pub_type: 杂志文章


    authors: Ghio M,Contini P,Negrini S,Mazzei C,Zocchi MR,Poggi A

    更新日期:2011-07-01 00:00:00

  • Anti-Dob implicated as the cause of a delayed hemolytic transfusion reaction.

    abstract::A 27-year-old, gravida 3, para 2 woman experienced a delayed hemolytic transfusion reaction. She had anti-Dob in both serum and eluate 8 days after infusion of 6 units of Do(b+) red cells. No antibody had been detected prior to transfusion. By the 15th day after transfusion, there was no evidence of survival of red ce...


    pub_type: 杂志文章


    authors: Moheng MC,McCarthy P,Pierce SR

    更新日期:1985-01-01 00:00:00

  • Lifeline for the front lines: blood products to support the warfighter.

    abstract:BACKGROUND:Hemorrhage is the leading cause of death on the battlefield. Damage control resuscitation guidelines in the US military recommend whole blood as the preferred resuscitation product. The Armed Services Blood Program (ASBP) has initiated low-titer group O whole blood (LTOWB) production and predeployment donor ...


    pub_type: 杂志文章


    authors: Taylor AL,Corley JB,Swingholm MT,Sloan MA,McDonald H Jr,Quesada JF,Evans CL,Ceballos WA

    更新日期:2019-04-01 00:00:00

  • The role of inflammation in intravenous immune globulin-mediated hemolysis.

    abstract::Intravenous immune globulin (IVIG) therapy has shown great success in a number of autoimmune and inflammatory conditions and its use continues to increase worldwide. There is growing awareness of significant side effects of high-dose IVIG: however, particularly severe hemolysis in patients that are non-group O. It has...


    pub_type: 杂志文章,评审


    authors: Pendergrast J,Willie-Ramharack K,Sampson L,Laroche V,Branch DR

    更新日期:2015-07-01 00:00:00

  • Clinical outcomes and transfusion management following intracranial hemorrhage in patients with acute leukemia.

    abstract:BACKGROUND:There is little evidence to guide management of patients with acute leukemia and intracranial hemorrhage (ICH). Predictors of long-term outcome following ICH are unknown. STUDY DESIGN AND METHODS:This study included adult patients with acute leukemia and ICH over an 8-year period. The primary outcome was da...


    pub_type: 杂志文章


    authors: Nixon S,Lieberman L,Atenafu EG,Doherty M,Murray C,Wolfe A,Brandys D,Rowland S,Kwan BY,Yu E,Lechner B,Maze D

    更新日期:2020-02-01 00:00:00

  • Prevalence and quantitation of parvovirus B19 DNA levels in blood donors with a sensitive polymerase chain reaction screening assay.

    abstract:BACKGROUND:Blood donor parvovirus B19 DNA prevalence with sensitive nucleic acid test assays has recently been demonstrated to be higher than that found with assays designed to detect high viral titers in the plasma manufacturing sector. STUDY DESIGN AND METHODS:Stored plasma aliquots from 5020 donations collected bet...


    pub_type: 杂志文章


    authors: Kleinman SH,Glynn SA,Lee TH,Tobler L,Montalvo L,Todd D,Kiss JE,Shyamala V,Busch MP,National Heart, Lung, Blood Institute Retrovirus Epidemiology Donor Study (REDS-II).

    更新日期:2007-10-01 00:00:00

  • Estimated risk of Chikungunya viremic blood donation during an epidemic on Reunion Island in the Indian Ocean, 2005 to 2007.

    abstract:BACKGROUND:Between 2005 and 2007, Chikungunya virus (CHIKV) caused a massive epidemic on Reunion Island with a major peak in the number of cases in February 2006. Blood donation was interrupted on the island in January 2006. STUDY DESIGN AND METHODS:Estimates of the mean risk of viremic blood donation on Reunion Islan...


    pub_type: 杂志文章


    authors: Brouard C,Bernillon P,Quatresous I,Pillonel J,Assal A,De Valk H,Desenclos JC,workgroup \Quantitative Estimation of the Risk of Blood Donation Contamination by Infectious Agents\.

    更新日期:2008-07-01 00:00:00

  • A novel GYPB-A-B hybrid gene responsible for Ss and MN typing discrepancies.

    abstract:BACKGROUND:Transfusion support of S-s- patients is very challenging but can now be alleviated by genotyping mutations in the GYPB gene to predict their U- or U+(var) phenotype. However, the phenotype predicted by genotyping does not always correspond to the observed phenotype of red blood cells (RBCs), which requires f...


    pub_type: 杂志文章


    authors: Willemetz A,Nataf J,Peyrard T,Arnaud L

    更新日期:2015-11-01 00:00:00

  • Prospective surveillance of D- recipients of D+ apheresis platelets: alloimmunization against D is not detected.

    abstract:BACKGROUND:Recent retrospective studies indicate that D- recipients of D+ apheresis platelets (PLTs) are not alloimmunized to D. Our hospital policy is to offer RhIG to D- women of childbearing age who received D+ apheresis PLTs but not to other D- recipients of D+ apheresis PLTs. We instituted prospective surveillance...


    pub_type: 杂志文章


    authors: Weinstein R,Simard A,Ferschke J,Vauthrin M,Bailey JA,Greene M

    更新日期:2015-06-01 00:00:00

  • Clinical significance of RBC alloantibodies and autoantibodies in sickle cell patients who received transfusions.

    abstract:BACKGROUND:The clinical significance of alloimmunization to RBC antigens in sickle cell patients was analyzed by a retrospective review of the records of pediatric and adult sickle cell patients who received transfusions and who were followed over a 10-year period. STUDY DESIGN AND METHODS:Charts of pediatric and adul...


    pub_type: 杂志文章,多中心研究,评审


    authors: Aygun B,Padmanabhan S,Paley C,Chandrasekaran V

    更新日期:2002-01-01 00:00:00

  • The use of polymerase chain reaction in plasma pools for the concomitant detection of hepatitis C virus and HIV type 1 RNA.

    abstract:BACKGROUND:Detection of viral nucleic acids might increase blood transfusion safety through the detection of recently infected blood donors during the preseroconversion window period. Individual screening is difficult to apply, because of technical and financial constraints. STUDY DESIGN AND METHODS:A polymerase chain...


    pub_type: 杂志文章


    authors: Yerly S,Pedrocchi M,Perrin L

    更新日期:1998-10-01 00:00:00

  • Fatal erythroderma (suspected graft-versus-host disease) after cholecystectomy. Retrospective analysis.

    abstract::Fatal postoperative erythroderma (POE) developed in a 52-year-old woman with gallstones who underwent elective cholecystectomy. During surgery, she was transfused with 3 units of unirradiated packed red cells stored in the liquid state for at least 4 days after collection. The POE is believed to have been the result o...


    pub_type: 杂志文章


    authors: Otsuka S,Kunieda K,Hirose M,Takeuchi H,Mizutani Y,Nagaya M,Sato G,Kasuya S,Matsutomo K,Noma A

    更新日期:1989-07-01 00:00:00

  • A comprehensive program to minimize platelet outdating.

    abstract:BACKGROUND:Platelet (PLT) transfusions are essential for patients who are bleeding or have an increased risk of bleeding due to a decreased number or abnormal function of circulating PLTs. A shelf life of 5 days for PLT products presents an inventory management challenge. In 2006, greater than 10% of apheresis PLTs mad...


    pub_type: 杂志文章


    authors: Fuller AK,Uglik KM,Braine HG,King KE

    更新日期:2011-07-01 00:00:00

  • Safe fetal platelet genotyping: new developments.

    abstract:BACKGROUND:Fetal and neonatal alloimmune thrombocytopenia (FNAIT) is due to maternal alloimmunization against fetal platelet (PLT) antigens. Antenatal management strategies have been developed to avoid complications such as intracranial hemorrhage. The aim of this study was to set up two reliable, noninvasive fetal gen...


    pub_type: 杂志文章


    authors: Le Toriellec E,Chenet C,Kaplan C

    更新日期:2013-08-01 00:00:00

  • Blood group A immunodeterminants on human red cells differ in biologic activity and sensitivity to alpha-N-acetylgalactosaminidase.

    abstract:BACKGROUND:Epitopes of blood group A antigen can be enzymatically cleaved from red cells (RBCs), but the extent of cleavage required for normal survival in allogeneic blood transfusion recipients is unknown. Therefore, the cleavage rates were studied for A antigen epitope binding of 1) complement-activating anti-A, 2) ...


    pub_type: 杂志文章


    authors: Hoskins LC,Larson G,Naff GB

    更新日期:1995-10-01 00:00:00

  • Duffy antigen changes on red blood cells stored at low temperature.

    abstract::Red blood cells stored in saline solution for two weeks at 12 C lose some of their Fya, Fyb, and Fy3 antigenicity. At the same time substances with specific inhibitory activity against appropriate Duffy antibodies appear in the saline. Similar changes could not be recognized in time-expired commercial reagent red bloo...


    pub_type: 杂志文章


    authors: Williams D,Johnson CL,Marsh WL

    更新日期:1981-05-01 00:00:00

  • An automatic wash method for dimethyl sulfoxide removal in autologous hematopoietic stem cell transplantation decreases the adverse effects related to infusion.

    abstract:BACKGROUND:Products cryopreserved with dimethyl sulfoxide (DMSO) in stem cell transplant (SCT) often cause many adverse effects during their infusion (major cardiovascular events, dyspnea … even death). These are especially frequent in pediatric patients. We tested if a fully automated and closed wash procedure (Sepax ...


    pub_type: 临床试验,杂志文章


    authors: Sánchez-Salinas A,Cabañas-Perianes V,Blanquer M,Majado MJ,Insausti CL,Monserrat J,Sánchez-Ibáñez MV,Menchón P,García-Hernández A,Gómez-Espuch J,Morales A,Moraleda JM

    更新日期:2012-11-01 00:00:00

  • Laboratory diagnosis of heparin-associated thrombocytopenia and comparison of platelet aggregation test, heparin-induced platelet activation test, and platelet factor 4/heparin enzyme-linked immunosorbent assay.

    abstract:BACKGROUND:As clinical diagnosis of heparin-associated thrombocytopenia (HAT) is often difficult, confirmation by sensitive laboratory assays is desirable. STUDY DESIGN AND METHODS:The sensitivity of the heparin-induced platelet activation (HIPA) test and the platelet aggregation test (PAT) was prospectively compared ...


    pub_type: 杂志文章


    authors: Greinacher A,Amiral J,Dummel V,Vissac A,Kiefel V,Mueller-Eckhardt C

    更新日期:1994-05-01 00:00:00

  • Blood transfusions recruit the microcirculation during cardiac surgery.

    abstract:BACKGROUND:Perioperative red blood cell transfusions are commonly used in patients undergoing cardiac surgery to correct anemia caused by blood loss and hemodilution associated with cardiopulmonary bypass circulation. The aim of this investigation was to test the hypothesis that blood transfusion has beneficial effects...


    pub_type: 杂志文章


    authors: Yuruk K,Almac E,Bezemer R,Goedhart P,de Mol B,Ince C

    更新日期:2011-05-01 00:00:00

  • Bloodless medicine: current strategies and emerging treatment paradigms.

    abstract:BACKGROUND:Advances in our understanding of the risks associated with allogeneic blood transfusions (ABTs) and the growing number of patients who wish to avoid ABTs have led to the emergence of new treatment paradigms for "bloodless" medicine and surgery. STUDY DESIGN AND METHODS:Here, we review prior studies and summ...


    pub_type: 杂志文章,评审


    authors: Resar LM,Wick EC,Almasri TN,Dackiw EA,Ness PM,Frank SM

    更新日期:2016-10-01 00:00:00

  • Effect of storage time and donor sex of transfused red blood cells on 1-year survival in patients undergoing cardiac surgery: an observational study.

    abstract:BACKGROUND:Red blood cell (RBC) storage lesions and RBCs from females transfused into male recipients may have adverse effects on transfusion recipients' survival. We hypothesized that the effect of donor sex and the effect of age of blood on mortality would be most apparent in cardiac surgery patients. STUDY DESIGN A...


    pub_type: 杂志文章,多中心研究


    authors: Desmarets M,Bardiaux L,Benzenine E,Dussaucy A,Binda D,Tiberghien P,Quantin C,Monnet E

    更新日期:2016-05-01 00:00:00

  • Murine monoclonal antibodies associated with Rh17, Rh29, and Rh46 antigens.

    abstract::After immunization with native human red cells and red cells infected with Plasmodium falciparum, mouse splenocytes were fused with a myeloma cell line to obtain hybridomas. Among the antibodies specific for blood group antigens, four antibodies (iB3C4, F12, MR432, and iB4) directed against epitopes related to the Rh ...


    pub_type: 杂志文章


    authors: Rouger P,Edelman L

    更新日期:1988-01-01 00:00:00

  • Evaluation of commercial antiglobulin sera over a two-year period. Part I. Anti-beta 1A, anti-alpha 2D, and anti-beta 1E levels.

    abstract::Antiglobulin sera from nine different manufacturers have been tested, over a two-year period, for their ability to detect the complement components beta 1A, alpha 2D, and beta 1E. The results demontrate considerable variation in the abilities of sera from different manufacturers to detect these components and indicate...


    pub_type: 杂志文章


    authors: Issitt PD,Issitt CH,Wilkinson SL

    更新日期:1974-03-01 00:00:00

  • Invasive line placement in critically ill patients: do hemostatic defects matter?

    abstract:BACKGROUND:Blood components are often given prophylactically before the placement of invasive lines in patients with coagulation defects. Little, however, is known about the epidemiology of defects in these patients. The purpose of this study is to ascertain what proportion of intensive care patients who receive invasi...


    pub_type: 杂志文章


    authors: DeLoughery TG,Liebler JM,Simonds V,Goodnight SH

    更新日期:1996-09-01 00:00:00

  • NAT screening of blood donors for severe acute respiratory syndrome coronavirus can potentially prevent transfusion associated transmissions.

    abstract:BACKGROUND:The severe acute respiratory syndrome (SARS) was first described in February 2003. Close contact with symptomatic patients appears to be the main route of transmission, whereas blood transfusion transmission could not be ruled out. STUDY DESIGN AND METHODS:A SARS coronavirus (SARS-CoV) detection kit develop...


    pub_type: 杂志文章


    authors: Schmidt M,Brixner V,Ruster B,Hourfar MK,Drosten C,Preiser W,Seifried E,Roth WK

    更新日期:2004-04-01 00:00:00

  • Transfusion of banked red blood cells and the effects on hemorrheology and microvascular hemodynamics in anemic hematology outpatients.

    abstract:BACKGROUND:The aim of this study was to investigate the effects of red blood cell (RBC) transfusion on the hemorrheologic properties and microcirculatory hemodynamics in anemic hematology outpatients receiving 2 to 4 RBC units of either "fresh" (leukoreduced storage for less than 1 week) or "aged" (leukoreduced storage...


    pub_type: 临床试验,杂志文章


    authors: Yürük K,Milstein DM,Bezemer R,Bartels SA,Biemond BJ,Ince C

    更新日期:2013-06-01 00:00:00