HPC viability measurement: trypan blue versus acridine orange and propidium iodide.

Abstract:

BACKGROUND:A reliable, validated method for rapidly determining HPC viability is essential for clinical cell engineering. STUDY DESIGN AND METHODS:A fluorometric cell viability assay using acridine orange and propidium iodide (AO/PI) was compared to the current standard, trypan blue (TB) exclusion. Viable cells stained with AO/PI fluoresce green under darkfield fluorescence microscopy, while nonviable cells fluoresce orange. Mixtures of fresh and heat-killed bone marrow were prepared and used as viability standards for evaluation of both assays. The frequency of CFU-GM was determined for each specimen. RESULTS:Cell viability measured by AO/PI was extremely linear, with measured and predicted viability in agreement from 0 to 100 percent of the viable cells and a coefficient of regression (r(2)) of 0.9921. The predicted-viability regression line fell within the 95% CI for AO/PI-measured viability. The coefficient of regression for TB-measured viability was 0.9584, with the predicted-viability regression line almost entirely outside the 95% CI. TB overestimated the percentage of viable cells, particularly below the 50-percent level. CFU-GM frequency correlated better with cell viability measured by AO/PI (r(2) = 0.979) than with that measured by TB (r(2) = 0.930). CONCLUSIONS:The AO/PI viability assay is a rapid, highly linear, functionally correlated assay that is superior to conventional viability measurement by TB exclusion.

journal_name

Transfusion

journal_title

Transfusion

authors

Mascotti K,McCullough J,Burger SR

doi

10.1046/j.1537-2995.2000.40060693.x

subject

Has Abstract

pub_date

2000-06-01 00:00:00

pages

693-6

issue

6

eissn

0041-1132

issn

1537-2995

journal_volume

40

pub_type

杂志文章
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    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2009.02203.x

    authors: Schmidt M,Korn K,Nübling CM,Chudy M,Kress J,Horst HA,Geusendam G,Hennig H,Sireis W,Rabenau HF,Doerr HW,Berger A,Hourfar MK,Gubbe K,Karl A,Fickenscher H,Tischer BK,Babiel R,Seifried E,Gürtler L

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    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2005.00611.x

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  • Evaluation of two viral inactivation methods for the preparation of safer factor VIII and factor IX concentrates.

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    pub_type: 杂志文章

    doi:10.1111/trf.13021

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    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2008.01793.x

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    doi:10.1111/trf.13731

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    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1992.32792391043.x

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    更新日期:1992-09-01 00:00:00

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    pub_type: 杂志文章,评审

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    pub_type: 杂志文章

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    pub_type: 杂志文章

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    pub_type: 杂志文章

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    journal_title:Transfusion

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    doi:

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    pub_type: 杂志文章

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    pub_type: 杂志文章,多中心研究,随机对照试验

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    pub_type: 杂志文章

    doi:10.1111/trf.16021

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