Protein quality in Mirasol pathogen reduction technology-treated, apheresis-derived fresh-frozen plasma.

Abstract:

BACKGROUND:The Mirasol pathogen reduction technology (PRT) system for plasma is based on a riboflavin (vitamin B(2)) and ultraviolet (UV) light treatment process resulting in pathogen inactivation due to irreversible photo-oxidative damage of nucleic acids. The purpose of this study was to evaluate the in vitro protein quality of apheresis-derived plasma treated with riboflavin and UV light in comparison with untreated fresh-frozen plasma (FFP). STUDY DESIGN AND METHODS:Twenty apheresis plasma samples (270 + or - 10 mL) were combined with 35 + or - 5 mL of riboflavin solution (500 microM), yielding a mean 60 microM final riboflavin concentration, and then exposed to UV light (6.24 J/mL). Riboflavin and UV light-treated plasma was then flash frozen, within 8 hours of collection, generating treated FFP. Treated FFP was thawed and analyzed using standard coagulation assays, and the percent retention of protein activity was reported, relative to untreated, paired controls. RESULTS:Plasma proteins demonstrated different sensitivities to riboflavin and UV treatment. The amount of total protein remained unchanged. After treatment, fibrinogen (antigen) showed 99% retention; Factor (F)XII, FXIII, ADAMTS-13, and von Willebrand factor (ristocetin cofactor) 96% to 100%. Fibrinogen retained 77% activity, FII 80%, FVIIIc 75%, and FV 73% after treatment. Antithrombin, protein S, plasminogen, and alpha(2)-antiplasmin retained between 91 and 100% activity. CONCLUSION:The results from this study demonstrate that coagulant and anticoagulant proteins in riboflavin and UV light-treated (PRT) apheresis plasma are well preserved.

journal_name

Transfusion

journal_title

Transfusion

authors

Smith J,Rock G

doi

10.1111/j.1537-2995.2009.02517.x

subject

Has Abstract

pub_date

2010-04-01 00:00:00

pages

926-31

issue

4

eissn

0041-1132

issn

1537-2995

pii

TRF2517

journal_volume

50

pub_type

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