A comparison of results obtained by two different chromium-51 methods of determining platelet survival and recovery.

Abstract:

:A number of different methods exist that use chromium-51 to determine platelet survival and recovery. A 1986 article in TRANSFUSION described the recommended methods for conducting such studies in the hope of standardizing methodology and permitting interlaboratory comparison of results. The results obtained with the recommended method have been compared with those of our previous method. With the new method, recovery of fresh random-donor platelets prepared in polyolefin bags was 76 +/- 6 percent with a survival of 9.3 +/- 2.8 days (n = 4). After 5 days of storage, these values were 67 +/- 8 percent and 7.8 +/- 1.9 days. Similar values were obtained for fresh and stored platelets collected on automated cell separators. However, when our previous (pre-1986) method of evaluation was used, recovery of fresh platelets in the polyolefin bags was 56 +/- 11 percent, and survival was 8.6 +/- 1.3 days. The 5-day values were 36 +/- 5 percent and 6.9 +/- 2.7 days. These values are significantly lower (p less than 0.05) than those obtained with the newer method, but they are in agreement with those previously reported for other techniques. Thus, the new method consistently gives higher values, possibly as a result of a less harmful labeling procedure.

journal_name

Transfusion

journal_title

Transfusion

authors

Rock G,Tittley P

doi

10.1046/j.1537-2995.1990.30590296371.x

subject

Has Abstract

pub_date

1990-06-01 00:00:00

pages

407-10

issue

5

eissn

0041-1132

issn

1537-2995

journal_volume

30

pub_type

杂志文章
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