Abstract:
BACKGROUND:Chagas disease is caused by Trypanosoma cruzi, a protozoan parasite that can be transmitted by transfusion. The diagnosis of chronic T. cruzi infection is generally made by detecting specific antibodies that bind to parasite antigens. The aim of this study was to assess the sensitivity and specificity of a new serologic assay for antibodies to T. cruzi on a fully automated analyzer (PRISM, Abbott Laboratories). STUDY DESIGN AND METHODS:A prototype chemiluminescent immunoassay based on chimeric recombinant antigens and run on the automated PRISM system was developed for detecting antibodies to T. cruzi in human serum and plasma. Assay specificity was evaluated by testing samples from random blood donors and from a diverse group of specimens from persons with diseases or conditions often associated with false-positive reactions in T. cruzi assays. Sensitivity was determined by testing 377 geographically diverse T. cruzi antibody-positive specimens. RESULTS:Six of 7911 samples (0.08%) from random donors were repeatedly reactive in the prototype PRISM Chagas assay. One of these was reactive in three other tests, including the radioimmune precipitation assay and was presumed to be a true positive. Hence, the specificity was 99.94 percent (7905/7910) in the negative donor group studied. All 377 T. cruzi antibody-positive specimens were positive in the prototype assay and thus the sensitivity was 100 percent. CONCLUSION:The results obtained to date, in terms of sensitivity as well as specificity, strongly suggest that the PRISM Chagas assay should function well as a tool for screening blood for serologic evidence of T. cruzi infection.
journal_name
Transfusionjournal_title
Transfusionauthors
Chang CD,Cheng KY,Jiang LX,Salbilla VA,Haller AS,Yem AW,Bryant JD,Kirchhoff LV,Leiby DA,Schochetman G,Shah DOdoi
10.1111/j.1537-2995.2006.00965.xsubject
Has Abstractpub_date
2006-10-01 00:00:00pages
1737-44issue
10eissn
0041-1132issn
1537-2995pii
TRF00965journal_volume
46pub_type
杂志文章,随机对照试验相关文献
TRANSFUSION文献大全abstract:BACKGROUND:Screening blood units for hepatitis C virus (HCV) with nucleic acid testing (NAT) reduces the risk associated with the long "window period" (8-9 weeks) after HCV infection. The feasibility of adding the HCV core antigen assay in pools to the existing anti-HCV individual screening was examined as an alternati...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2006.00972.x
更新日期:2006-10-01 00:00:00
abstract:BACKGROUND:AABB Standard 5.27 requires transfusion services to have a process for urgent release of blood before completion of compatibility testing. Our institution endorses a policy for the emergency release of group O, D+ red blood cells (RBC; O+ RBC) to males and females at least 50 years of age. Our emergency depa...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12925
更新日期:2015-04-01 00:00:00
abstract:BACKGROUND:Patients with severe combined immunodeficiency (SCID) treated with allogeneic bone marrow transplantation often receive a milder conditioning regimen than patients who undergo transplantation for hematologic malignancy, and they regularly retain circulating white cells of host origin. The origin of circulati...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1996.36496226143.x
更新日期:1996-04-01 00:00:00
abstract:BACKGROUND:Currently, donors may arrive at blood collection sites without prior knowledge of eligibility and deferral criteria. STUDY DESIGN AND METHODS:The effects of distributing newly developed recruitment brochures 2 weeks in advance of blood drives and the provision of brochures on temporary deferral at the takin...
journal_title:Transfusion
pub_type: 临床试验,杂志文章
doi:10.1046/j.1537-2995.1994.34794330012.x
更新日期:1994-07-01 00:00:00
abstract:BACKGROUND:Babesia microti, the most frequently implicated pathogen in transfusion-transmitted babesiosis, is widely endemic in the Northeast and upper Midwestern United States. High seroprevalence in endemic areas limits antibody-based donor screening. A high-performance molecular test is needed to identify donors in ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12098
更新日期:2013-10-01 00:00:00
abstract:BACKGROUND:Platelet (PLT) transfusions are essential for patients who are bleeding or have an increased risk of bleeding due to a decreased number or abnormal function of circulating PLTs. A shelf life of 5 days for PLT products presents an inventory management challenge. In 2006, greater than 10% of apheresis PLTs mad...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2010.03039.x
更新日期:2011-07-01 00:00:00
abstract:BACKGROUND:The Verax PGD rapid test for bacteria in platelets (PLTs) has been updated to simplify workflow and improve specificity and sensitivity by employing a novel sequential format. The performance of this updated version, called PGDprime, was evaluated to determine its suitability for use as an FDA-cleared "safet...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.16000
更新日期:2020-11-01 00:00:00
abstract::Sixty English-language articles on the motivation and recruitment of blood donors and nondonors are reviewed and evaluated. Apparently researchers have been finding essentially the same results for approximately twenty years. Motivations to donate are: altruism/humanitarian, personal or family credit, social pressure,...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1977.17277151916.x
更新日期:1977-03-01 00:00:00
abstract:BACKGROUND:ABO-incompatible red blood cell (RBC) transfusions and acute hemolytic reactions occur infrequently, yet resultant fatalities are reported to the US Food and Drug Administration (FDA) every year. We describe a 20-year retrospective study of reported mistransfusion cases to identify temporal trends, common ca...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.16121
更新日期:2020-12-01 00:00:00
abstract::Cell pheresis was employed in the initial management of a pregnant patient with chronic myelocytic leukemia (CML). Reduction in circulating leukocyte and platelet counts was evident after two weeks of therapy. This technique is an effective mode of management of the pregnant patient with CML and may play an important ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1978.18178118555.x
更新日期:1978-01-01 00:00:00
abstract:BACKGROUND:RBCs can be frozen with either the high-glycerol method (HGM) or the low-glycerol method (LGM). To date, the use of frozen RBCs is hampered by a 24-hour outdating period after thawing. A closed washing system (ACP 215) may solve this problem. STUDY DESIGN AND METHODS:We compared the effects of high- (40%) a...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2003.00293.x
更新日期:2003-02-01 00:00:00
abstract:BACKGROUND:The anti-CD20 monoclonal antibody rituximab has immune-modulatory effects similar to intravenous immunoglobulin (IVIG). We performed a systematic review and meta-analysis to determine the efficacy and safety of rituximab in autoimmune diseases that are also treated with IVIG. STUDY DESIGN AND METHODS:The mo...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14841
更新日期:2018-11-01 00:00:00
abstract::Sterility of blood products is a cardinal contributor to patient safety. Bacteriologic controls of stable products comply with strict regulations, but legislation imposes only limited constraints in the case of perishable products, such as packed red cells (RBCs) or fresh-frozen plasma (FFP). Therefore, it is essentia...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1986.26386209392.x
更新日期:1986-05-01 00:00:00
abstract:BACKGROUND:The relationship between length of storage of red blood cell (RBC) units and biochemical changes has been well studied, but little is known about the progression of cellular immunomodulative properties in blood recipients. This study aims to quantify in vitro T-cell activation and cytokine release by white b...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2009.02319.x
更新日期:2009-11-01 00:00:00
abstract:BACKGROUND:People overestimate the likelihood that blood donors will experience faint and prefaint reactions, particularly if they are themselves fearful of having blood drawn. This study investigated the influence of making a risk assessment for oneself versus others, and examined whether providing information about t...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15225
更新日期:2019-06-01 00:00:00
abstract::A new blood pack system for the preparation of white cell-depleted red cells was studied. The system is a modified additive-solution quadruple-unit blood pack that incorporates a cellulose-acetate fiber depth filter in-line between the AS-3 additive bag and the CP2D collection bag. Mean +/- SD white cell removal from ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1989.29689318446.x
更新日期:1989-07-01 00:00:00
abstract:BACKGROUND:Borrelia miyamotoi, a recently discovered relapsing fever spirochete, occurs in hard-bodied ticks wherever Lyme disease is endemic. Human infection is associated with relapsing fever and can cause meningoencephalitis in immunocompromised patients. A few cases of transfusion transmission of other relapsing fe...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12879
更新日期:2015-03-01 00:00:00
abstract:BACKGROUND:In August 2016, the Food and Drug Administration advised US blood centers to screen all whole blood and apheresis donations for Zika virus (ZIKV) with an individual-donor nucleic acid test (ID-NAT) or to use approved pathogen reduction technology (PRT). The cost of implementing this guidance nationally has n...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14164
更新日期:2017-06-01 00:00:00
abstract:BACKGROUND:Autoimmune hemolytic anemia (AIHA) is usually classified as either warm or cold type. During the past few decades, mixed types (Mxs) have also been described in a number of cases (6%-8% of AIHA), often without serologic data to support the diagnosis. In this study, we demonstrate that the incidence of Mx AIH...
journal_title:Transfusion
pub_type: 杂志文章,评审
doi:10.1111/j.1537-2995.2008.01805.x
更新日期:2008-10-01 00:00:00
abstract:BACKGROUND:Deterioration in quality of platelet concentrates (PCs) during storage results from the appearance of storage lesions affecting the hemostatic functions and posttransfusion survival of platelets. These lesions depend on the preparation and pathogen inactivation methods used, duration of storage, and platelet...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.16280
更新日期:2021-02-01 00:00:00
abstract:BACKGROUND:Red blood cell exchange (RCE) transfusions are a mainstay in the treatment of sickle cell anemia (SCA), and allow a temporary restoration of physiological parameters with respect to erythrocyte oxygen carrying capacity and systems metabolism. Recently, we noted that 1) RCE significantly impacts recipients' m...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15467
更新日期:2019-10-01 00:00:00
abstract:BACKGROUND:There is little evidence to guide management of patients with acute leukemia and intracranial hemorrhage (ICH). Predictors of long-term outcome following ICH are unknown. STUDY DESIGN AND METHODS:This study included adult patients with acute leukemia and ICH over an 8-year period. The primary outcome was da...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15621
更新日期:2020-02-01 00:00:00
abstract::The ability to store platelets beyond 24 hours requires a functionally closed system. This study tested the ability of a cell separator bowl seal system to resist penetration of microbial contamination under normal running conditions and under extreme environmental stress. Three test organisms, Micrococcus luteus, Ser...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1990.30290162901.x
更新日期:1990-02-01 00:00:00
abstract::The development of flexible plastic blood bags has permitted effective blood component production and therapy. However, the plasticizer di(2-ethylhexyl)phthalate (DEHP), whose toxicity in humans is still undefined, is known to leach from the plastic into stored blood. Despite the availability of bags made of plastics ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1984.24685066808.x
更新日期:1984-11-01 00:00:00
abstract:BACKGROUND:Direct oral anticoagulants (DOACs) are effective and safe for prophylaxis and treatment of thromboembolic phenomena. However, managing DOACs during bleeding emergencies is challenging. A systematic review and meta-analysis was conducted on studies addressing efficacy and safety of the drugs used for reversal...
journal_title:Transfusion
pub_type: 杂志文章,meta分析,评审
doi:10.1111/trf.14096
更新日期:2017-07-01 00:00:00
abstract::Ultraviolet B (UVB) irradiation of platelet concentrates (PCs) may prevent the development of posttransfusion HLA alloimmunization. As irradiation performed in a blood center or a hospital will probably be associated with a variable postirradiation delay before transfusion, the ability to store PCs after UVB irradiati...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1991.31691306243.x
更新日期:1991-07-01 00:00:00
abstract::Evidence supporting the existence of two agents of human non-A, non-B hepatitis was obtained by the inoculation of chimpanzees sequentially with serum from a chronically infected human (Inoculum I) and with fibrinogen prepared from pooled plasma (Inoculum IV), each of which had transmitted non-A, non-B hepatitis to hu...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1984.24384225027.x
更新日期:1984-05-01 00:00:00
abstract:BACKGROUND:Some jurisdictions require leukoreduction of cellular blood components. The only whole blood collection set with a platelet-saving filter uses citrate-phosphate-dextrose (CPD) as storage solution. Substituting CPD with citrate-phosphate-dextrose-adenine (CPDA-1) increases shelf life from 21 to 35 days. This ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15748
更新日期:2020-05-01 00:00:00
abstract:BACKGROUND:Selection of CD34+ PBPCs has been applied as a method of reducing graft contamination from neoplastic cells. This procedure seems to delay lymphocyte recovery, while myeloid engraftment is no different from that with unselected PBPC transplants. STUDY DESIGN AND METHODS:Lymphocyte recovery was studied in tw...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2001.41060783.x
更新日期:2001-06-01 00:00:00
abstract::We have estimated the risk of transmitting HIV infection from a unit of HIV seronegative blood collected in Los Angeles and Orange Counties, CA from March 1985 through February 1987. Our method consisted of lookback investigations and anti-HIV testing of living recipients of HIV seronegative units donated by persons w...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1988.28588337347.x
更新日期:1988-09-01 00:00:00