Abstract:
BACKGROUND:The proposal to assess the viability capabilities of platelets (PLTs) collected, treated, or stored in a developmental system against "fresh" PLTs from the same subject poses several important methodologic issues pertaining to the timing and manner of the collecting and separating the fresh PLTs. This study extended the previous validation of this method of comparing fresh and stored PLTs, applying it to an assessment of apheresis PLTs stored for 7 days with a newly standardized radiolabeling protocol. STUDY DESIGN AND METHODS:Eighteen normal subjects donated 1 unit of leukoreduced PLTs, pheresed with a standard, approved system. They received an aliquot radiolabeled with 51Cr on Day 7 simultaneously with 111In-labeled fresh PLTs that had been separated by a manual method. Recovery and survival were compared to determine whether the stored PLTs were not inferior to the criterion of 67 percent of recovery and 50 percent of survival of fresh PLTs. Separate studies were undertaken to document the similarity of recovery and survival with 51Cr and 111In radiolabeling in PLTs stored to 8 days and to determine the importance of correcting the radioactivity in timed samples for the activity remaining in blood beyond the life span of the retransfused PLTs. RESULTS:PLTs stored for 7 days demonstrated 88.7 +/- 35.2 percent of the recovery and 89.9 +/- 21.2 percent of the survival of PLTs collected via a nonproprietary, manual system and thus met the comparative criterion. In a separate study (n = 12), labeling Day 8 PLTs with 51Cr or 111In resulted in recoveries and survivals that were not different. Radiolabel eluted from labeled PLTs in vitro was taken up by cellular blood elements in a reuptake incubation. CONCLUSION:Apheresis PLTs stored for 7 days met the criterion proposed for comparison with fresh PLTs. This analytic approach is feasible with PLTs collected and prepared via a manual method. A standardized protocol for radiolabeling PLTs with 51Cr and 111In and analyzing the results in a standardized fashion was employed successfully, with the two radioisotopes yielding similar results. The importance of correcting for residual activity after disappearance of injected cells was noted.
journal_name
Transfusionjournal_title
Transfusionauthors
AuBuchon JP,Herschel L,Roger Jdoi
10.1111/j.1537-2995.2005.00177.xsubject
Has Abstractpub_date
2005-07-01 00:00:00pages
1143-50issue
7eissn
0041-1132issn
1537-2995pii
TRF00177journal_volume
45pub_type
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