Abstract:
BACKGROUND:An optimal cooling rate is critical for cryopreserving cord blood stem cells, so it is generally achieved by using controlled-rate freezers (CRFs). The cooling rate can be interrupted by many factors during cooling; therefore, a proper response to the interruption in cryopreservation is crucial to prevent the loss of valuable cells. STUDY DESIGN AND METHODS:Cord blood samples (n = 6) containing 10% dimethyl sulfoxide were cooled from 4 to -80°C using a CRF. At different temperatures during cooling, cells were transferred either to liquid nitrogen vapor phase (LNVP) directly or a -80°C mechanical freezer where they were stored for 18.1 ± 0.6 hours before transferring to LNVP. The cells were stored in LNVP for 127 ± 48.1 hours before postthaw recovery, viability, and survival of total nucleated cells and/or CD45+ cells, CD34+ cells, and colony-forming unit-granulocyte-macrophage (CFU-GM) were assessed. RESULTS:For all types of postthaw evaluations, there were no significant differences when cells were transferred to a -80°C freezer before LNVP, regardless of the temperature at which cooling was interrupted. In contrast, there were significant differences when cells were transferred to LNVP directly, but the differences were determined by the type of assays and how viability was calculated. CONCLUSIONS:Cord blood samples can be transferred to a -80°C freezer anytime during controlled-rate cooling, but should only be transferred to LNVP when the samples have been cooled to -40°C or lower. Percent postthaw survival of CFU-GM should be routinely assessed when controlled-rate cooling is interrupted.
journal_name
Transfusionjournal_title
Transfusionauthors
Yang H,Pidgorna A,Loutfy MR,Shuen Pdoi
10.1111/trf.12774subject
Has Abstractpub_date
2015-01-01 00:00:00pages
70-8issue
1eissn
0041-1132issn
1537-2995journal_volume
55pub_type
杂志文章相关文献
TRANSFUSION文献大全abstract:BACKGROUND:In sub-Saharan Africa, the percentage of screened blood is limited to approximately 75 percent for human immunodeficiency virus antibodies (anti-HIV), 50 percent for hepatitis B surface antigen, and 19 percent for hepatitis C virus antibodies (anti-HCV), mainly because of costs. STUDY DESIGN AND METHODS:In ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2004.04279.x
更新日期:2005-02-01 00:00:00
abstract::The whole blood electrical conductivity method for predonation hematocrit determinations was studied. Forty capillary and venous blood samples were tested concurrently with electrical conductivity and centrifugation methods. The electrical conductivity method demonstrated acceptable accuracy but was less precise than ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1985.25385219908.x
更新日期:1985-05-01 00:00:00
abstract::A procedure is described for the large-scale production of pure suspensions of human lymphocytes and granulocytes. Platelet-poor buffy-coats, obtained by centrifugation of six units of ABO-compatible ACD blood, were pooled and centrifuged again, resulting in a crude leukocyte concentrate containing approximately 60 pe...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1977.17578014593.x
更新日期:1977-09-01 00:00:00
abstract:BACKGROUND:In December 2015, the men who have sex with men (MSM) deferral was reduced to 12 months in the United States. We compared human immunodeficiency virus (HIV), hepatitis C virus (HCV), and hepatitis B virus (HBV) incidence and residual risk before and after this policy change using data from >50% of the US blo...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.16250
更新日期:2021-01-18 00:00:00
abstract:BACKGROUND:This study was designed to evaluate the potential yield of introducing nucleic acid amplification testing (NAT) in blood donation, according to the detection threshold and the pool size. STUDY DESIGN AND METHODS:A mathematical model of early HIV-1 population dynamics in blood has been developed and is used ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1999.39101141.x
更新日期:1999-10-01 00:00:00
abstract:BACKGROUND:Demonstration of viable Chlamydia (Chlamydophila) pneumoniae in peripheral blood mononuclear cells (PBMNCs) is essential to understand the involvement of C. pneumoniae in atherosclerosis. Nevertheless, the prevalence of viable C. pneumoniae in the blood of healthy donors has not yet been studied. STUDY DESI...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2004.04005.x
更新日期:2004-07-01 00:00:00
abstract:BACKGROUND:Fever accompanying vaso-occlusive crisis is a common presentation in patients with sickle cell disease (SCD) and carries a broad differential diagnosis. Here, we report a case of transfusion-transmitted malaria in a patient with SCD presenting with acute vaso-occlusive crisis and rapidly decompensating to mu...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14566
更新日期:2018-06-01 00:00:00
abstract:BACKGROUND:Alloimmunization against human platelet antigens (HPAs) during pregnancy is rare but can lead to severe bleeding disorders, such as fetal and neonatal alloimmune thrombocytopenia. STUDY DESIGN AND METHODS:In a cohort of 241 uncomplicated pregnancies, we investigated the immunogenicity of HPA mismatches and ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14053
更新日期:2017-05-01 00:00:00
abstract:BACKGROUND:The aim was to assess iron status and dietary iron intake in a sample of premenopausal female regular and new blood donors. STUDY DESIGN AND METHODS:Premenopausal women blood donors were invited to participate. Blood samples were analyzed for serum ferritin and hemoglobin. An iron checklist assessed dietary...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12347
更新日期:2014-03-01 00:00:00
abstract::A unit-dose system was designed to dispense precise quantities of blood in a form ready for immediate transfusion into neonatal patients. The principles were similar to those used by pharmacies to dispense individual doses of drugs. In the blood center, the precise volume of blood ordered for a neonatal patient was as...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1986.26286152907.x
更新日期:1986-03-01 00:00:00
abstract::A pilot study was performed on the storage of plasma and cryosupernatant plasma at 4 degrees C for up to 28 days. Eight bags, four of CPD fresh-frozen plasma (FFP) and four of CPD cryosupernatant plasma (CSP, plasma without cryoprecipitate), were sampled during storage for assays of pH; factors V, VIII, IX, and XI; fi...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1993.33994025023.x
更新日期:1993-09-01 00:00:00
abstract::A polyolefin plastic (PL 732) bag formulated without liquid plasticizer allows storage of platelets for 5 and, now, up to 7 days. In order to assess the leaching of compounds from this new plastic, extracts of the supernatant from platelet concentrates stored in these bags were analyzed by high-performance liquid chro...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1986.26486262743.x
更新日期:1986-07-01 00:00:00
abstract:BACKGROUND:Advances in the past two decades in dendritic cell (DC) biology paved the way to exploit them as a promising tool in cancer immunotherapy. The prerequisite for DC vaccine preparations is large-scale in vitro generations of homogeneous, mature, and functional DCs. Frequent improvements are being made in the e...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2010.02706.x
更新日期:2010-11-01 00:00:00
abstract:INTRODUCTION:Frozen plasma is superior to crystalloids for hemorrhage resuscitation but remains logistically challenging in austere environments because of specialized clinical equipment for on-demand thawing. This research examines some ad hoc thawing techniques that have been implemented by military medical personnel...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15652
更新日期:2020-06-01 00:00:00
abstract::Vancomycin, an antibiotic similar in structure to ristocetin, is used to treat staphylococcal infections. However, vancomycin-induced hemagglutination complicated red cell (RBC) serologic testing in the blood bank. At concentrations greater than 3.0 mg per ml, vancomycin caused spontaneous macroscopic red cell (RBC) a...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1989.29189101158.x
更新日期:1989-01-01 00:00:00
abstract::Donation willingness is generally high but registering with donor centers lags behind. We need to understand why and when during the life course which groups of individuals are motivated to donate. With this knowledge, we can design targeted recruitment and retention strategies and improve evidence-based donor managem...
journal_title:Transfusion
pub_type: 杂志文章,评审
doi:10.1111/trf.15268
更新日期:2019-06-01 00:00:00
abstract:BACKGROUND:Hypothermic storage transforms red blood cells (RBC) from smooth biconcave discocytes into increasingly spherical spiculated echinocytes and, ultimately, fragile spherocytes (S). Individual cells undergo this transformation at different rates, producing a heterogeneous mixture of RBCs at all stages of echino...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15979
更新日期:2020-08-04 00:00:00
abstract::The accumulation of the plasticizer di-2-ethylhexylphthalate (DEHP) in blood and blood components has been of considerable concern for some time. We have followed the accumulation of DEHP and one of its major metabolities, mono-2-ethylhexylphthalate (MEHP) during storage of whole blood, platelet-rich plasma, platelet ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1978.18579036383.x
更新日期:1978-09-01 00:00:00
abstract:BACKGROUND:Drug-induced immune pancytopenia is considered an uncommon disorder. CASE REPORT:A 76-year-old woman with metastatic gastric adenocarcinoma received 15 cycles of FOLFOX-6 (oxaliplatin/folinic acid/fluorouracil) with a complete response. Upon disease progression, she was restarted on FOLFOX; during the seven...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2010.02600.x
更新日期:2010-07-01 00:00:00
abstract:BACKGROUND:Severe transfusion-related acute lung injury is often caused by antibodies directed against the human neutrophil alloantigen (HNA)-3a. HNA-3a results from an amino acid exchange (Arg154Gln) in the first extracellular loop of the choline transporter-like protein 2 (CTL2). The characteristics of the binding do...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2011.03115.x
更新日期:2011-10-01 00:00:00
abstract:BACKGROUND:Using umbilical cord blood (UCB) cells, it was demonstrated that three virulent isolates, two highly transmissible clinical isolates and the virulent laboratory strain, demonstrated rapid growth in the UCB cells, which was significantly faster than the growth rate observed for a unique isolate. There was als...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2009.02187.x
更新日期:2009-08-01 00:00:00
abstract:BACKGROUND:Preoperative autologous blood donation (PABD) has been used to reduce the need for allogeneic RBC transfusion, decreasing risk and conserving supply. A frozen PABD program for heart transplant patients was instituted at the Mayo Clinic in 1988, but participation has steadily declined. The aims of this study ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2003.00357.x
更新日期:2003-04-01 00:00:00
abstract:BACKGROUND:Cytomegalovirus (CMV) infections in very-low-birthweight infants can lead to serious clinical consequences. When CMV-related symptoms occur after transfusion, CMV transmission is often attributed to the transfusion products rather than to breast milk. However, it is sometimes difficult to distinguish between...
journal_title:Transfusion
pub_type:
doi:10.1111/trf.13547
更新日期:2016-06-01 00:00:00
abstract::KEY IDEAS Chimeric antigen receptor (CAR) T-cell therapy has the potential to improve the dismal outcome of patients diagnosed with acute myeloid leukemia (AML). A major challenge for CAR T-cell therapy of AML patients is identifying leukemia-specific target antigens. Immune escape through down-regulation of target an...
journal_title:Transfusion
pub_type: 杂志文章,评审
doi:10.1111/trf.15199
更新日期:2019-04-01 00:00:00
abstract::Anti-Emm, an antibody to a public red cell antigen, was found in the serums of four Emm-propositi and in the serum of the Emm-brother of one of them. Three of the propositi were men, and their antibodies, as well as that of the compatible brother, were believed to be "naturally occurring." Since there are two Emm-sibl...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1987.27487264738.x
更新日期:1987-07-01 00:00:00
abstract:BACKGROUND:Epitopes of blood group A antigen can be enzymatically cleaved from red cells (RBCs), but the extent of cleavage required for normal survival in allogeneic blood transfusion recipients is unknown. Therefore, the cleavage rates were studied for A antigen epitope binding of 1) complement-activating anti-A, 2) ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1995.351096026361.x
更新日期:1995-10-01 00:00:00
abstract:BACKGROUND:The purpose of this study was to explore the molecular basis of the p phenotype by analysis of the recently cloned 4-alpha-galactosyltransferase gene responsible for synthesis of Pk (Gb3) antigen. STUDY DESIGN AND METHODS:Forty samples from individuals of eight different nationalities were investigated by s...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2003.00425.x
更新日期:2003-07-01 00:00:00
abstract:BACKGROUND:Cell-free fetal DNA in maternal plasma opens the way for routine risk-free diagnosis of fetal D status of D- mothers. The focus was on accuracy of RHD typing and confirmation of fetal DNA in maternal plasma while RHD was not detected. STUDY DESIGN AND METHODS:Plasma DNA was extracted (by manual and/or autom...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2005.00559.x
更新日期:2005-09-01 00:00:00
abstract:BACKGROUND:JMH is a high-frequency red cell blood group antigen that resides on a 76- to 80-kDa glycosylphosphatidylinositol-linked protein also known as CDw108. Antibodies with JMH specificity are often autoimmune and are usually, if not always, clinically benign. Some individuals with JMH-variant antigen produce allo...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1995.351196110897.x
更新日期:1995-11-01 00:00:00
abstract:BACKGROUND:Etoricoxib is a selective inhibitor of cyclooxygenase 2 used mainly to treat osteoarticular pain. Here, we report the case of a patient who developed acute kidney failure and immune hemolytic anemia after the use of etoricoxib. STUDY DESIGN AND METHODS:An 83-year-old female patient developed immune hemolyti...
journal_title:Transfusion
pub_type:
doi:10.1111/trf.15226
更新日期:2019-05-01 00:00:00