Abstract:
:In colorectal cancer (CRC), KRAS and BRAF mutations in primary tumors are associated with resistance to anti-epidermal growth factor receptor (anti-EGFR)-based therapies. However, the correlation between KRAS/BRAF mutation in primary tumors and serum has not been well studied. To evaluate the degree of concordance of KRAS/BRAF mutations between the primary tumors and the matched serum samples in CRC, serum and tumor tissues were collected from 115 patients with CRC and KRAS/BRAF mutations were examined by nested polymerase chain reaction (PCR) and direct sequencing. BRAF mutations were present in 3.5% (4/115) of the primary tumor tissue samples and 0.87% (1/115) of the serum samples. In the 4 primary tumors with BRAF mutations, identical mutations were not observed in the corresponding serum samples (κ=-0.016). KRAS mutations were observed in 32.2% (37/115) of the primary tumors and 11.3% (13/115) of the serum samples. Of the 37 tumor cases with KRAS mutations, 9 had identical mutations in the corresponding serum sample, with a concordance rate of 24.3% (9/37). Discordance was observed in 32 (27.8%) patients. The concordance between KRAS mutations in the primary tumors and KRAS mutations in the matched serums was low (κ=0.231). The results of the present study suggest that the possibility of differences in the mutational status of KRAS/BRAF between primary tumors and matched serum samples should be considered when patients are selected for anti-EGFR-based therapies.
journal_name
Oncol Lettjournal_title
Oncology lettersauthors
Pu X,Pan Z,Huang Y,Tian Y,Guo H,Wu L,He X,Chen X,Zhang S,Lin Tdoi
10.3892/ol.2012.963subject
Has Abstractpub_date
2013-01-01 00:00:00pages
249-254issue
1eissn
1792-1074issn
1792-1082pii
ol-05-01-0249journal_volume
5pub_type
杂志文章相关文献
Oncology Letters文献大全abstract::Despite all efforts at management, prognosis of advanced lung cancer is extremely poor, with a median survival time of ~1 year. The number of cancer patients aged >70 years is significantly increased among the cancer patient population. The aim of this study was to investigate the clinical importance of age in lung ca...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2013.1566
更新日期:2013-11-01 00:00:00
abstract::Sorafenib is a multikinase inhibitor and is effective in treating hepatocellular carcinoma (HCC). However, it remains unknown whether sorafenib induces the alteration of protein glycosylation. The present study treated HCC MHCC97L and MHCC97H cells with a 50% inhibitory concentration of sorafenib. Following this treat...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.6177
更新日期:2017-07-01 00:00:00
abstract::The transcription factor AP-2 β (TFAP2B) serves an important role in kidney development. MicroRNAs (miRNAs) regulate carcinogenic pathways and have gained increasing attention owing to their association with human clear cell renal cell carcinoma (ccRCC) tumorigenesis. However, whether miRNAs could affect renal cell tu...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2020.12187
更新日期:2020-12-01 00:00:00
abstract::The incidence of gastric cancer is high, especially in China. The present study aims to provide a novel therapeutic target for gastric cancer. Peripheral blood, cancerous and paracancerous tissues were collected from patients with gastric cancer. T-cell immunoglobulin mucin domain-3 (Tim-3) expression in T-cells was m...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.9743
更新日期:2019-02-01 00:00:00
abstract::The present case study reported of amelanotic malignant melanoma of the esophagus. A 68-year-old man underwent laparoscopic distal gastrectomy for early gastric cancer diagnosis. After gastrectomy, endoscopic examination revealed a protruded lesion lying adjacent to the melanosis area of the esophagus. Histology of th...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8479
更新日期:2018-06-01 00:00:00
abstract::Substantial evidence implicates that low-abundance cancer stem cells (CSCs) are responsible for tumor metastasis and recurrence in hepatocellular carcinoma (HCC). Side population (SP) cells possess typical CSCs-like features, and are frequently considered as a special subpopulation in which CSCs are enriched and in st...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.4343
更新日期:2016-05-01 00:00:00
abstract::It has been widely reported that patient-derived tumor xenografts (PDXs) are more similar to tumor tissues than conventional cancer cell lines. Kinetochore-associated protein 2 (KNTC2) is known to be upregulated specifically in tumor tissues of cancer patients and is recognized as a potential target for cancer therapy...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.7890
更新日期:2018-04-01 00:00:00
abstract::The aim of the present study was to observe the in vivo targeting characteristic of angiopoietin 2-small interfering RNA (Ang2-siRNA) plasmid/chitosan magnetic nanoparticles in an established nude mouse model of malignant melanoma (MM) under an external magnetic field. The nude mouse MM model was first established, th...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.6443
更新日期:2017-08-01 00:00:00
abstract::Colorectal cancer (CRC) is the third leading cause of cancer-associated mortalities. Long non-coding RNAs (lncRNAs) have been identified as key regulators in the occurrence and development of CRC. The lncRNA urothelial cancer associated 1 (UCA1) has been demonstrated to promote the development of numerous different ty...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.10737
更新日期:2019-10-01 00:00:00
abstract::Salivary gland carcinomas are rare tumors, representing ~0.5% of all malignancies. Myoepithelioma is also uncommon, representing ~1% of all salivary gland tumors. Myoepithelial carcinoma (MC) is even rarer, representing 0.2 to 0.6% of all salivary gland tumors. We herein report a case of MC with multiple metastases ar...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.5783
更新日期:2017-04-01 00:00:00
abstract::Breast metastasis is a rare pattern of distant metastasis from nasopharyngeal carcinoma (NPC) following curative treatment. The present study reported two patients with breast metastasis from NPC and reviewed four such patients reported in the literature, including the analysis of clinical profiles, pathological diagn...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.3818
更新日期:2015-12-01 00:00:00
abstract::Endocrine-disrupting chemicals (EDCs) are exogenous chemical compounds ubiquitously found in everyday life of the modern world. EDCs enter the human body where they act similarly to endogenous hormones, altering the functions of the endocrine system and causing adverse effects on human health. Bisphenol A (BPA), the p...
journal_title:Oncology letters
pub_type: 杂志文章,评审
doi:10.3892/ol.2020.12145
更新日期:2020-12-01 00:00:00
abstract::Although tumor thrombus (TT) infringement of the inferior vena cava (IVC) and right atrium (RA) is rarely observed in hepatocellular carcinoma (HCC), the prognosis for this condition is extremely poor, with a median survival time of several months, given that the condition is often diagnosed at an advanced tumor stage...
journal_title:Oncology letters
pub_type: 杂志文章,评审
doi:10.3892/ol.2020.11962
更新日期:2020-10-01 00:00:00
abstract::Targeted therapy of non-small cell lung cancer (NSCLC) patients with mutations in the epidermal growth factor receptor (EGFR) gene has been associated with improved prognosis. However, there is a shortage on data from real-world clinical practice in management of EGFR-positive NSCLC patients in Poland. The present stu...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.6907
更新日期:2017-11-01 00:00:00
abstract::The aim of the current study was to investigate the potential role of microRNA-183-5p (miR-183-5p) in the proliferation, invasion and metastasis of pancreatic cancer, and to identify promising target genes of oncogenic miR-183-5p. Western blotting and quantitative polymerase chain reaction (qPCR) were used to investig...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.3872
更新日期:2016-01-01 00:00:00
abstract::Exosomes have gained increased research focus due to their key roles as messengers. The components of exosomes include proteins and RNAs that may be horizontally transferred between adjacent or distant cells. Hepatocellular carcinoma (HCC) is among the most malignant types of cancer worldwide, with exosomes implicated...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.7257
更新日期:2017-12-01 00:00:00
abstract::Septin 2 (SEPT2) is a tumor-related gene belonging to the SEPT family that affects the cellular processes of hepatoma carcinoma cells, glioblastoma cells and mesangial cells and is highly expressed in breast cancer, biliary tract cancer and acute myeloid leukemia. Colorectal cancer (CRC) is the third most common type ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.10528
更新日期:2019-09-01 00:00:00
abstract::The present study aimed to explore the diagnostic and prognostic value of lncRNA maternally expressed 3 (MEG3) in cervical cancer. Eighty-four patients with cervical cancer from February 2013 to March 2014 were enrolled in the observation group (OG), and another 58 female subjects who underwent physical examination at...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2020.12044
更新日期:2020-11-01 00:00:00
abstract::Nasopharyngeal carcinoma (NPC) is a common tumor in south China. Kinesin family member 2A (KIF2A) belongs to the kinesin-13 family and is associated with the growth and invasion of a number of different types of human cancer, including ovarian, breast and prostate cancer. The aim of the present study was to evaluate t...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.10597
更新日期:2019-09-01 00:00:00
abstract::Pyruvate kinase isoenzyme M2 (PKM2) has previously been identified as a tumor biomarker and potential therapeutic target for the treatment of cancer. In the present study, FFJ-3, a structurally modified version of mollugin, an extract of the Traditional Chinese herbal medicine Rubia tinctorum (madder) was used in orde...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.5761
更新日期:2017-04-01 00:00:00
abstract::Hedgehog (HH) and Wnt pathway activation have been implicated in poor prognosis of breast cancer. Crosstalk between these two pathways has been demonstrated to be important in breast cancer progression, however the association between these two pathways and breast cancer survival rate is unknown. The present study com...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.6874
更新日期:2017-11-01 00:00:00
abstract::The epithelial-mesenchymal transition (EMT) process has increasingly been examined due to its role in the progression of human tumors. Renal cell carcinoma (RCC) is one of the most common urological tumors that results in patient mortality. Previous studies have demonstrated that the EMT process is closely associated ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.4900
更新日期:2016-09-01 00:00:00
abstract::The present study aimed to ascertain the significance of topoisomerase II α (TOP2A) and minichromosome maintenance protein (MCM) 2 expression in anal carcinoma. A total of 75 anal lesions were retrieved from the files of the Department of Pathology of Barretos Cancer Hospital (Barretos, Brazil) in order to verify the ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.5650
更新日期:2017-03-01 00:00:00
abstract::The expression level and roles of microRNA-497 (miR-497) have been frequently reported in previous studies on cancer. However, its expression, function and associated molecular mechanisms in retinoblastoma remain unknown. In the present study, miR-497 expression levels in human retinoblastoma tissues, normal retinal t...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.6083
更新日期:2017-06-01 00:00:00
abstract::The present study aimed to examine the effectiveness of immunostaining of cytological specimens in discerning squamous from non-squamous cell carcinoma. Various combinations of six antibodies were examined in order to determine the optimal combination for use in differential diagnosis. Immunostaining was performed usi...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.3045
更新日期:2015-05-01 00:00:00
abstract::Discovering the underlying signalling pathways that control cancer cells is crucial for understanding their biology and to develop therapeutic regimens. Thus, the aim of the present study was to determine the effect of Cripto-1 on pathways controlling glioblastoma (GBM) cell function. To this end, changes in protein p...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.10626
更新日期:2019-09-01 00:00:00
abstract::Aberrant expression of Ras homolog enriched in brain (RHEB) has been observed in a variety of cancer tissues and is closely associated with clinicopathological features. However, the expression profile of RHEB in patients with hepatocellular carcinoma (HCC) and its clinical signature with underlying mechanisms have no...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.7759
更新日期:2018-03-01 00:00:00
abstract::In this study, we report the cytogenetic analysis of a 31-year-old male with a rare translocation between chromosomes 1 and 16 and inversion of chromosome 5 in CD99-positive soft tissue sarcoma of the thigh, which metastasized to the lung. Histologically, the tumor showed ovoid or short-spindle atypical cells with pos...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.641
更新日期:2012-06-01 00:00:00
abstract::Cisplatin-resistant ovarian cancer occurs in patients with ovarian cancer treated with cisplatin-based chemotherapy, which results in tumor progression during treatment, or recurrence of the tumor within 6 months of the treatment. It is vital that a novel biomarker for diagnosis, or an efficient therapeutic target of ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8433
更新日期:2018-06-01 00:00:00
abstract::Euchromatic histone-lysine N-methyltransferase (G9A), the primary histone methyltransferase for histone H3 Lys9, has been identified to be upregulated in numerous types of cancer. The aim of the present study was to analyze the clinical significance of G9A, and preliminarily explore its function in hepatocellular carc...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8572
更新日期:2018-06-01 00:00:00