Abstract:
:Far-upstream element-binding protein 2 (FBP2) is an internal ribosomal entry site (IRES) trans-acting factor (ITAF) that negatively regulates enterovirus 71 (EV71) translation. This study shows that EV71 infection cleaved FBP2. Live EV71 and the EV71 replicon (but not UV-inactivated virus particles) induced FBP2 cleavage, suggesting that viral replication results in FBP2 cleavage. The results also showed that virus-induced proteasome, autophagy, and caspase activity co-contribute to EV71-induced FBP2 cleavage. Using FLAG-fused FBP2, we mapped the potential cleavage fragments of FBP2 in infected cells. We also found that FBP2 altered its function when its carboxyl terminus was cleaved. This study presents a mechanism for virus-induced cellular events to cleave a negative regulator for viral IRES-driven translation.
journal_name
J Viroljournal_title
Journal of virologyauthors
Chen LL,Kung YA,Weng KF,Lin JY,Horng JT,Shih SRdoi
10.1128/JVI.02278-12subject
Has Abstractpub_date
2013-04-01 00:00:00pages
3828-38issue
7eissn
0022-538Xissn
1098-5514pii
JVI.02278-12journal_volume
87pub_type
杂志文章abstract::The terminal 500-base-pair alpha sequence of the herpes simplex virus 1 genome contains signals for cleavage (Pac1 and Pac2) of unit-length DNA molecules from concatemers in unique stretches of sequences designated Ub and Uc, respectively, and a cis site for cleavage designated DR1. We report that nuclear extracts fro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.3.1059-1068.1989
更新日期:1989-03-01 00:00:00
abstract::A complete endogenous type D viral genome has been isolated from a baboon genomic library. The provirus, simian endogenous retrovirus (SERV), is 8,393 nucleotides long and contains two long terminal repeats and complete genes for gag, pro, pol, and env. The primer binding site is complementary to tRNA(Lys)3, like in l...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.5.3666-3676.1997
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abstract::Human immunodeficiency virus type 2 (HIV-2), like other lentiviruses, is capable of infecting nondividing T cells and macrophages. The present work shows that in HIV-2-infected cells, Vpx is necessary for efficient nuclear import of the preintegration complex. In agreement with this finding, the subcellular localizati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.13.6162-6167.2000
更新日期:2000-07-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) is a gammaherpesvirus that has been implicated in the pathogenesis of Kaposi's sarcoma. KSHV encodes K-bZIP (open reading frame K8), a protein that belongs to the basic region-leucine zipper (bZIP) family of transcription factors. Here we show that K-bZIP associates with ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.24.11977-11982.2000
更新日期:2000-12-01 00:00:00
abstract::In intact Madin-Darby canine kidney (MDCK) cell monolayers, vesicular stomatitis virus (VSV) matures only at basolateral membranes beneath tight junctions, whereas influenza virus buds from apical cell surfaces. Early in the growth cycle, the viral glycoproteins are restricted to the membrane domain from which each vi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.40.3.848-860.1981
更新日期:1981-12-01 00:00:00
abstract::The interaction of the mouse hepatitis virus (MHV) nucleocapsid protein (N) and viral RNA was examined. Monoclonal antibody specific for N protein coimmunoprecipitated MHV genomic RNA as well as all six MHV subgenomic mRNAs found in MHV-infected cells. In contrast, monoclonal antibodies to the MHV E2 or E1 envelope gl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.11.4280-4287.1988
更新日期:1988-11-01 00:00:00
abstract::The family Caliciviridae is divided into four genera and consists of single-stranded RNA viruses with hosts ranging from humans to a wide variety of animals. Human caliciviruses are the major cause of outbreaks of acute nonbacterial gastroenteritis, whereas animal caliciviruses cause various host-dependent illnesses w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.12.6469-6479.2004
更新日期:2004-06-01 00:00:00
abstract::HLA-B*52:01 is strongly associated with protection against HIV disease progression. However, the mechanisms of HLA-B*52:01-mediated immune control have not been well studied. We here describe a cohort with a majority of HIV C-clade-infected individuals from Delhi, India, where HLA-B*52:01 is highly prevalent (phenotyp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02025-19
更新日期:2020-06-16 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.7.4971-4980.1997
更新日期:1997-07-01 00:00:00
abstract::The fusion (F) protein of simian virus 5 (strain W3A) induced extensive cell fusion in BHK cells when expressed alone, while that of strain WR did not. Mutational analysis demonstrated that the fusing activity can be transferred to the WR F protein by a proline residue at position 22 of subunit W3A F2. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.12.9855-9858.1997
更新日期:1997-12-01 00:00:00
abstract::Recombinant viruses possessing reporter proteins have been generated for virus research. In the case of the family Flaviviridae, we recently generated recombinant viruses, including the hepatitis C virus of the genus Hepacivirus, Japanese encephalitis virus (JEV) of the genus Flavivirus, and bovine viral diarrhea viru...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01191-19
更新日期:2019-10-29 00:00:00
abstract::The protein product of the v-myb oncogene of avian myeloblastosis virus, p48v-myb, differs structurally in several ways from its normal cellular homolog, p75c-myb. We demonstrated that the 11 specific amino acid substitutions found in two independent molecular clones of this virus were not required for the transformat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.3.1093-1096.1988
更新日期:1988-03-01 00:00:00
abstract::Papillomavirus disease poses a special challenge to people with compromised immune systems. Appropriate models to study infections in these individuals are lacking. We report here the development of a model that will help to address these deficiencies. The MmuPV1 genome was synthesized and used successfully to produce...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00777-13
更新日期:2013-08-01 00:00:00
abstract::The pregenomic RNA (pgRNA) of hepatitis B virus (HBV) serves as the messenger for both core and P proteins, with the downstream P gene translated by ribosomal leaky scanning. HBV replication begins with packaging of the pgRNA and P protein into core protein particles, followed by conversion of RNA into DNA. Genotype G...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05583-11
更新日期:2011-12-01 00:00:00
abstract::We previously described a human immunodeficiency virus type 1 (HIV-1) envelope mutant that introduces a disulfide bridge between the gp120 surface proteins and gp41 transmembrane proteins (J. M. Binley, R. W. Sanders, B. Clas, N. Schuelke, A. Master, Y. Guo, F. Kajumo, D. J. Anselma, P. J. Maddon, W. C. Olson, and J. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.10.5678-5684.2003
更新日期:2003-05-01 00:00:00
abstract::The relationship between virus-specific CD8(+)-T-cell responses and viral persistence was studied in mice by using Hantaan virus (HTNV). We first established a simple method for measuring levels of virus-specific CD8(+) T cells by flow cytometry. Next, to produce a mouse model of persistent HTNV infection, newborn mic...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.15.8408-8417.2003
更新日期:2003-08-01 00:00:00
abstract::Ultrastructure was correlated with growth kinetics of bdellophage VL-1 infecting host-dependent ("parasitic") Bdellovibrio bacteriovorus 109J in its Escherichia coli B host (the three-membered system), as well as in the host-independent ("saprophytic") derivative of the Bdellovibrio. Electron microscope observations s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.6.1522-1533.1973
更新日期:1973-12-01 00:00:00
abstract::Our previous studies have argued persuasively that in murine sarcoma virus ts110 (MuSVts110) the gag and mos genes are fused out of frame due to a approximately 1.5-kilobase (kb) deletion of wild-type murine sarcoma virus 349 (MuSV-349) viral information. As a consequence of this deletion, infected cells grown at 39 d...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.53.2.624-633.1985
更新日期:1985-02-01 00:00:00
abstract::Sequences of the human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) domain were determined by direct sequencing of HIV-1 RNA in successive plasma samples from eight seroconverting patients infected with virus bearing the T215Y/F amino acid substitution associated with zidovudine (ZDV) resistance. A...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.5.3520-3523.1998
更新日期:1998-05-01 00:00:00
abstract::Three polyadenylated RNAs, 9, 7.3, and 3.5 kilobases long, of a human endogenous retrovirus, ERV3, are abundant in human placental chorion, representing about 0.03 to 0.05% of the total mRNA. We characterized the structure of these mRNAs by Northern blot and S1 nuclease mapping analyses. We found that all three RNAs w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.7.2182-2191.1987
更新日期:1987-07-01 00:00:00
abstract::Alphaherpesviruses are parasites of the peripheral nervous system in their natural hosts. After the initial infection of peripheral tissues such as mucosal cells, these neurotropic viruses will invade the peripheral nervous system that innervates the site of infection via long-distance axonal transport of the viral ge...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.17.10875-10889.2005
更新日期:2005-09-01 00:00:00
abstract::We have developed a system for producing murine leukemia virus (MLV) pseudotyped with human hepatitis B virus (HBV) large (L) and small (S) surface antigens (HBsAg) for targeting primary human hepatocytes. Using the MLV(HBV) pseudotype virus containing a beta-galactosidase reporter gene, we demonstrated that this pseu...
journal_title:Journal of virology
pub_type: 杂志文章,收录出版
doi:10.1128/jvi.76.2.912-917.2002
更新日期:2002-01-01 00:00:00
abstract::The great majority of viral mRNAs in mouse C127 cells transformed by bovine papillomavirus type 1 (BPV) have a common 3' end at the early polyadenylation site which is 23 nucleotides (nt) downstream of a canonical poly(A) consensus signal. Twenty percent of BPV mRNA from productively infected cells bypasses the early ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.12.7705-7710.1993
更新日期:1993-12-01 00:00:00
abstract::The fraction of the adenovirus type 5 genome expressed as mRNA in a rat cell line, 5RK, transformed by the HsuI-G restriction fragment was analyzed by saturation hybridization using separated strands of 32P-labeled HindIII-G fragment. It was found that 45 to 50% of G fragment (about 8 X 10(5) daltons) is expressed as ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.28.3.1011-1014.1978
更新日期:1978-12-01 00:00:00
abstract::The largest tegument protein of herpes simplex virus 1 (HSV-1), UL36, contains a novel deubiquitinating activity embedded in it. All members of the Herpesviridae contain a homologue of HSV-1 UL36, the N-terminal segments of which show perfect conservation of those residues implicated in catalysis. For murine cytomegal...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.24.15582-15585.2005
更新日期:2005-12-01 00:00:00
abstract::Although highly active antiretroviral therapy (HAART) for human immunodeficiency virus type 1 (HIV-1) infection can reduce levels of HIV-1 RNA in plasma to below the limit of detection, replication-competent forms of the virus persist in all infected individuals. One form of persistence involves a stable reservoir of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.18.9481-9492.2002
更新日期:2002-09-01 00:00:00
abstract::Human cytomegalovirus encodes four unique short (US) region proteins, each of which is independently sufficient for causing the down-regulation of major histocompatibility complex (MHC) class I molecules on the cell surface. This down-regulation enables infected cells to evade recognition by cytotoxic T lymphocytes (C...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02397-05
更新日期:2006-06-01 00:00:00
abstract::Human herpesvirus 7 (HHV-7) is a recently isolated betaherpesvirus that is prevalent in the human population, with primary infection usually occurring in early childhood. HHV-7 is related to human herpesvirus 6 (HHV-6) in terms of both biological and, from limited prior DNA sequence analysis, genetic criteria. However...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.9.5975-5989.1996
更新日期:1996-09-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) lytic infection increases the expression of viral and human interleukin-6 (vIL-6 and hIL-6, respectively), an important factor for cell growth and pathogenesis. Here, we report genome-wide analysis of viral RNA targets of KSHV ORF57 by a novel UV-cross-linking and immunop...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02144-10
更新日期:2011-03-01 00:00:00
abstract::It was recently shown that the 7a protein of severe acute respiratory syndrome coronavirus induces biochemical changes associated with apoptosis. In this study, the mechanism by which the 7a protein induces apoptosis was examined. The 7a protein was tested for the ability to inhibit cellular gene expression because se...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.2.785-793.2006
更新日期:2006-01-01 00:00:00