Abstract：
:Most imaging studies of the enteric nervous system (ENS) that regulates the function of the gastrointestinal tract are so far performed using preparations isolated from animals, thus hindering the understanding of the ENS function in vivo. Here we report a method for imaging the ENS cellular network activity in living mice using a new transgenic mouse line that co-expresses G-CaMP6 and mCherry in the ENS combined with the suction-mediated stabilization of intestinal movements. With confocal or two-photon imaging, our method can visualize spontaneous and pharmacologically-evoked ENS network activity in living animals at cellular and subcellular resolutions, demonstrating the potential usefulness for studies of the ENS function in health and disease.
journal_name
Neurosci Resjournal_title
Neuroscience researchauthors
Motegi Y,Sato M,Horiguchi K,Ohkura M,Gengyo-Ando K,Ikegaya Y,Fusamae Y,Hongo Y,Suzuki M,Ogawa K,Takaki M,Nakai Jdoi
10.1016/j.neures.2019.02.004subject
Has Abstractpub_date
2020-02-01 00:00:00pages
53-60eissn
0168-0102issn
1872-8111pii
S0168-0102(18)30523-6journal_volume
151pub_type
杂志文章abstract：:How the nervous system controls the sensation and memory of information from the environment is an essential question. The nematode Caenorhabditis elegans is a useful model for elucidating neural information processing that mediates sensation and memory. The entire nervous system of C. elegans consists of only 302 neu...
journal_title：Neuroscience research
pub_type： 杂志文章,评审
doi：10.1016/j.neures.2013.03.008
更新日期：2013-07-01 00:00:00
abstract：:Projection fields in the striatum and claustrum from eye movement-evoking cerebral cortices (EMECs) in the cat were investigated using the WGA-HRP tracing method under electrophysiological guidance to place WGA-HRP in the appropriate sites. The EMECs in the frontal cortex, which are located in the medial wall under th...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/0168-0102(90)90033-b
更新日期：1990-08-01 00:00:00
abstract：:In an attempt to test the possibility that sino-aortic baroreceptors may mediate the previously reported stress response in hypothalamic magnocellular neurosecretory cell activity in rats, effects of deafferentation of sino-aortic baroreceptors on plasma levels of vasopressin and oxytocin after fear-related emotional ...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/0168-0102(92)90059-l
更新日期：1992-04-01 00:00:00
abstract：:Changes in voltage-dependent Na+ (INa) and Ca2+ currents (ICa) were investigated in PC12 cells cultured without or with either nerve growth factor (NGF) or 8-Br-cAMP for 10 days. Current recordings were made in the whole-cell mode by using the conventional patch-clamp technique under voltage-clamp conditions. Membrane...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/0168-0102(93)90081-z
更新日期：1993-02-01 00:00:00
abstract：:Environmental factors during perinatal period have various effects on behavior. The present study examined the effects of prenatal stress and neonatal handling on anxiety and spatial learning of offspring. Prenatal stress increased anxiety-related behavior of adult offspring, whereas neonatal handling had no effect. I...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/j.neures.2015.07.002
更新日期：2015-12-01 00:00:00
abstract：:The organization of the sagittal Zone C of the cerebellar cortex of the rat was studied with respect to its efferent projections and to its inferior olive (IO) afferent connections. Wheat-germ agglutinin conjugated to horseradish peroxidase was used as a tracer. Zone C has been defined as the cortical region projectin...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/0168-0102(88)90039-9
更新日期：1988-08-01 00:00:00
abstract：:The influence of the phosphorylation dephosphorylation states on the gamma-aminobutyric acid (GABA) transporter activity of synaptic plasma membranes (SPM) was studied by using either specific phosphatase inhibitors or activators. Calyculin A and okadaic acid (phosphatase 1 and phosphatase 2A inhibitors) inhibited the...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/s0168-0102(98)00107-2
更新日期：1999-01-01 00:00:00
abstract：:Small teleost fish including zebrafish and medaka have been used as animal models in basic science research due to the relative ease of handling and transparency during embryogenesis. Current advances in genetic engineering and progress in disease genetics allowed utilization of these fish to study neurological diseas...
journal_title：Neuroscience research
pub_type： 杂志文章,评审
doi：10.1016/j.neures.2017.02.004
更新日期：2017-07-01 00:00:00
abstract：:We investigated the neural mechanisms underlying the ability to cope in atypical or novel situations using tools. We hypothesized that two cognitive components support this ability: adaptive coordination (for adapting to situational demands) and cognitive inhibition (for inhibiting the incongruent actions afforded by ...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/j.neures.2014.03.008
更新日期：2015-01-01 00:00:00
abstract：:Properties of the Ca2+ -activated K+ current (I[K(Ca)]) were investigated in bipolar cells isolated from the goldfish retina. Pharmacological experiments and single channel current recordings demonstrated that I[K(Ca)] represented currents through BK channels, which were confined mostly to the presynaptic terminal. Th...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/s0168-0102(97)01155-3
更新日期：1997-03-01 00:00:00
abstract：:This study evaluates the efficacy of mifepristone on weight restoration in rats subjected to dietary restriction and methylphenidate administration. 25 female rats aged between 9 and 12 months were divided into 2 groups: 5 controls (exposed only to dietary restriction) and 20 rats that were administered 5 mg/kg/d of m...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/j.neures.2017.12.008
更新日期：2018-10-01 00:00:00
abstract：:Xenopus oocytes injected with messenger RNA extracted from rat brain are induced to acquire a variety of neurotransmitter receptors and voltage-operated membrane channels. Activation of the receptors to serotonin, acetylcholine (muscarinic) and glutamate elicits oscillatory membrane currents carried by chloride ions. ...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/0168-0102(85)90021-5
更新日期：1985-08-01 00:00:00
abstract：:[3H]Glutamic acid binding to hippocampi was increased after amygdaloid kindling in rats, and diazepam inhibited this increased binding, without any effect on the enhanced binding by CaCl2 or the binding in control rats. By inducing kindling in the same way as that used in the binding experiment, the inhibiting effects...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/0168-0102(87)90011-3
更新日期：1987-02-01 00:00:00
abstract：:The cyclic nucleotides perform a variety of roles in the formation and remodeling of the neuronal interaction. The membrane microdomain called "raft" has been paid much attention, for this domain contains many signal-transducing molecules including trimeric G proteins and cytoskeletal proteins. The raft domain is reco...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/s0168-0102(02)00208-0
更新日期：2003-02-01 00:00:00
abstract：:Following neonatal hypoxia-ischemia (HI) serotonin (5-hydroxytryptamine, 5-HT) levels are decreased in the brain. The regulation of brain 5-HT is dependent on the serotonin transporter (SERT) localised at the neuronal pre-synaptic cell membrane. However SERT can also traffic away from the cell membrane into the cytoso...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/j.neures.2012.04.002
更新日期：2012-07-01 00:00:00
abstract：:It has been postulated that horizontal cells (HCs) send feedback signals onto cones via a proton feedback mechanism, which generates the center-surround receptive field of bipolar cells, and color-opponent signals in many non-mammalian vertebrates. Here we used a strong pH buffer, HEPES, to reduce extracellular proton...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/j.neures.2012.01.008
更新日期：2012-04-01 00:00:00
abstract：:The subgranular zone (SGZ) in the dentate gyrus contains radial astrocytes, known as Type-1 or Type-B cells, which generate neuroblasts (Type-2 cells or Type-D cells) that give rise to granular neurons. Stress increases glucocorticoid levels that target SGZ and modify the proliferation and apoptosis of hippocampal cel...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/j.neures.2011.03.013
更新日期：2011-07-01 00:00:00
abstract：:γ-Aminobutyric acid (GABA) generally induces hyperpolarization and inhibition in the adult brain, but causes depolarization (and can be excitatory) in the immature brain. Depolarizing GABA actions are necessary for neurogenesis, differentiation, migration, and synaptogenesis. Additionally, the conversion of GABA respo...
journal_title：Neuroscience research
pub_type： 杂志文章,评审
doi：10.1016/j.neures.2020.02.010
更新日期：2020-07-01 00:00:00
abstract：:l-Serine is required for the synthesis of glycine and d-serine, both of which are NMDA receptor co-agonists. Although roles for d-serine and glycine have been suggested in schizophrenia, little is known about the role of the l-serine synthesizing cascade in schizophrenia or related psychiatric conditions. Here we repo...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/j.neures.2010.10.003
更新日期：2011-02-01 00:00:00
abstract：:We studied the ascending and descending axonal trajectories of excitatory vestibular neurons related to the anterior semicircular canal, by means of local stimulation and spike-triggered signal averaging techniques in anesthetized cats. More than 200 vestibular neurons related to the ampullary nerve of the anterior se...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/0168-0102(84)90036-1
更新日期：1984-10-01 00:00:00
abstract：:A study was made of the effects of taurine on GABArho1 receptors expressed in Xenopus oocytes. The EC(50) and reversal potentials for GABA, taurine and glycine currents were 2.3+/-0.4 microM (-25+/-0.9 mV), 5+/-0.8mM (-27+/-0.4 mV) and 7+/-0.5mM (-22+/-0.6 mV), respectively. Co-application of GABA and taurine, reveale...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/j.neures.2008.03.009
更新日期：2008-07-01 00:00:00
abstract：:Some evidence suggests that sleep deprivation might impair synaptic plasticity and produce oxidative stress in the hippocampus. However it is not clear whether impairment of long-term potentiation depends on the oxidative stress evoked by sleep deprivation protocol. In this study we aimed to investigate the effects of...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/j.neures.2011.01.008
更新日期：2011-05-01 00:00:00
abstract：:We previously demonstrated that metabotropic glutamate receptor-subtype 1 knockout [mGluR1 (-/-)] mice showed ataxic gait, deficient long-term depression and impaired synapse elimination and these phenotypes were rescued by introduction of an mGluR1 transgene with Purkinje cell-specific promoter (mGluR1-rescue mice). ...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/j.neures.2006.12.014
更新日期：2007-04-01 00:00:00
abstract：:To detect the release of nitric oxide (NO) in brain tissue, an electrochemical microprobe was developed. The output current of the probe correlated linearly with the NO concentration at the tip, and the sensitivity of the probe was between 3.5 and 106 pA per 1 microM change in NO concentration. This probe showed no se...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/0168-0102(90)90048-j
更新日期：1990-09-01 00:00:00
abstract：:Cytokines have been implicated in the etiology or pathology of various psychiatric diseases of developmental origin such as autism and schizophrenia. Leukemia inhibitory factor (LIF) is induced by a variety of brain insults and known to have many influences on mature and immature nervous system. Here, we assessed the ...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/j.neures.2003.12.001
更新日期：2004-03-01 00:00:00
abstract：:Metabolism of amyloid-beta peptide (Abeta) is closely associated with the pathology and etiology of Alzheimer's disease (AD). Since neprilysin is the only rate-limiting catabolic peptidase proven by reverse genetics to participate in Abeta metabolism in vivo, we performed detailed immunohistochemical analysis of nepri...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/s0168-0102(02)00015-9
更新日期：2002-05-01 00:00:00
abstract：:Prepulse inhibition (PPI) and habituation of the acoustic startle reflex (ASR) are considered to be candidate endophenotypes of schizophrenia. However, to our knowledge, only one group has investigated these startle measures in Asian patients with schizophrenia. In the present study, we evaluated these startle measure...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/j.neures.2008.08.006
更新日期：2008-11-01 00:00:00
abstract：:Fifteen rabbits were classically conditioned using stimulation of the right pontine nuclei as a conditioned stimulus and a corneal airpuff as an unconditioned stimulus. After conditioning criterion was reached, the stimulation conditioned stimulus was transferred to the left pontine nuclei and the rate of conditioned ...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/0168-0102(86)90057-x
更新日期：1986-09-01 00:00:00
abstract：:ATP-sensitive K(+) (K(ATP)) channel subunits SUR2A and SUR2B in the rat brain were investigated by RT-PCR assay, western blot analysis, in situ hybridization histochemistry, and immunohistochemical staining. The results show that the mRNA and protein of SUR2A and SUR2B are expressed in whole rat brain extracts and sel...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/j.neures.2012.08.005
更新日期：2012-10-01 00:00:00
abstract：:Dopamine is a major neurotransmitter in the carotid body of several animal species and its functional role at the level of peripheral arterial chemoreflex pathway is attributed to the presence of the dopamine D2-receptors. We present evidence that the dopamine D1-receptor mRNA is also expressed in the carotid body of ...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/s0168-0102(98)00033-9
更新日期：1998-06-01 00:00:00
