Abstract:
:Most imaging studies of the enteric nervous system (ENS) that regulates the function of the gastrointestinal tract are so far performed using preparations isolated from animals, thus hindering the understanding of the ENS function in vivo. Here we report a method for imaging the ENS cellular network activity in living mice using a new transgenic mouse line that co-expresses G-CaMP6 and mCherry in the ENS combined with the suction-mediated stabilization of intestinal movements. With confocal or two-photon imaging, our method can visualize spontaneous and pharmacologically-evoked ENS network activity in living animals at cellular and subcellular resolutions, demonstrating the potential usefulness for studies of the ENS function in health and disease.
journal_name
Neurosci Resjournal_title
Neuroscience researchauthors
Motegi Y,Sato M,Horiguchi K,Ohkura M,Gengyo-Ando K,Ikegaya Y,Fusamae Y,Hongo Y,Suzuki M,Ogawa K,Takaki M,Nakai Jdoi
10.1016/j.neures.2019.02.004subject
Has Abstractpub_date
2020-02-01 00:00:00pages
53-60eissn
0168-0102issn
1872-8111pii
S0168-0102(18)30523-6journal_volume
151pub_type
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