Confocal and multiphoton calcium imaging of the enteric nervous system in anesthetized mice.


:Most imaging studies of the enteric nervous system (ENS) that regulates the function of the gastrointestinal tract are so far performed using preparations isolated from animals, thus hindering the understanding of the ENS function in vivo. Here we report a method for imaging the ENS cellular network activity in living mice using a new transgenic mouse line that co-expresses G-CaMP6 and mCherry in the ENS combined with the suction-mediated stabilization of intestinal movements. With confocal or two-photon imaging, our method can visualize spontaneous and pharmacologically-evoked ENS network activity in living animals at cellular and subcellular resolutions, demonstrating the potential usefulness for studies of the ENS function in health and disease.


Neurosci Res


Neuroscience research


Motegi Y,Sato M,Horiguchi K,Ohkura M,Gengyo-Ando K,Ikegaya Y,Fusamae Y,Hongo Y,Suzuki M,Ogawa K,Takaki M,Nakai J




Has Abstract


2020-02-01 00:00:00












  • Revisiting a theory of cerebellar cortex.

    abstract::Long-term depression at parallel fiber-Purkinje cell synapses plays a principal role in learning in the cerebellum, which acts as a supervised learning machine. Recent experiments demonstrate various forms of synaptic plasticity at different sites within the cerebellum. In this article, we take into consideration syna...

    journal_title:Neuroscience research

    pub_type: 杂志文章,评审


    authors: Yamazaki T,Lennon W

    更新日期:2019-11-01 00:00:00

  • Na+/Ca2+ exchanger activity induces a slow DC potential after in vitro ischemia in rat hippocampal CA1 region.

    abstract::In rat hippocampal CA1 neurons recorded intracellularly from tissue slices, a rapid depolarization occurred approximately 5 min after application of ischemia-simulating medium. In extracellular recordings obtained from CA1 region, a rapid negative-going DC potential (rapid DC potential) was recorded, corresponding to ...

    journal_title:Neuroscience research

    pub_type: 杂志文章


    authors: Uchikado H,Tanaka E,Yamamoto S,Isagai T,Shigemori M,Higashi H

    更新日期:2000-02-01 00:00:00

  • Elucidation of molecular pathogenesis and drug development for psychiatric disorders from rare disease-susceptibility variants.

    abstract::Recent rapid progress in genome analysis and large-scale consortia has made it possible to discover variants with a variety of allele frequencies and effect sizes associated with psychiatric disorders. Among psychiatric disorder-susceptibility variants, rare variants with large effect sizes detected by sequencing anal...

    journal_title:Neuroscience research

    pub_type: 杂志文章,评审


    authors: Kimura H,Mori D,Aleksic B,Ozaki N

    更新日期:2020-12-11 00:00:00

  • Stress by noise produces differential effects on the proliferation rate of radial astrocytes and survival of neuroblasts in the adult subgranular zone.

    abstract::The subgranular zone (SGZ) in the dentate gyrus contains radial astrocytes, known as Type-1 or Type-B cells, which generate neuroblasts (Type-2 cells or Type-D cells) that give rise to granular neurons. Stress increases glucocorticoid levels that target SGZ and modify the proliferation and apoptosis of hippocampal cel...

    journal_title:Neuroscience research

    pub_type: 杂志文章


    authors: Gonzalez-Perez O,Chavez-Casillas O,Jauregui-Huerta F,Lopez-Virgen V,Guzman-Muniz J,Moy-Lopez N,Gonzalez-Castaneda RE,Luquin S

    更新日期:2011-07-01 00:00:00

  • Pericyte function in the physiological central nervous system.

    abstract::Damage to the central nervous system (CNS) leads to disruption of the vascular network, causing vascular dysfunction. Vascular dysfunction is the major event in the pathogenesis of CNS diseases and is closely associated with the severity of neuronal dysfunction. The suppression of vascular dysfunction has been conside...

    journal_title:Neuroscience research

    pub_type: 杂志文章,评审


    authors: Muramatsu R,Yamashita T

    更新日期:2014-04-01 00:00:00

  • Effects of nocturnal bright light on saliva melatonin, core body temperature and sleep propensity rhythms in human subjects.

    abstract::Nine healthy male volunteers (mean age of 24) participated in two experimental sessions of random crossover design: a bright light (5000 lux for 5 h from 00:00 to 05:00 h) session and a dim light (10 lux for 5 h from 00:00 to 05:00 h) session. Subsequently participants entered an ultra-short sleep-wake schedule for 26...

    journal_title:Neuroscience research

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Kubota T,Uchiyama M,Suzuki H,Shibui K,Kim K,Tan X,Tagaya H,Okawa M,Inoue S

    更新日期:2002-02-01 00:00:00

  • Anisotropic functional connections between the auditory cortex and area 18a in rat cerebral slices.

    abstract::We developed a new method to visualize the myeloarchitecture in fresh slices, and investigated the properties of the functional neural connections around the boundary between the primary auditory cortex (area 41) and area 18a in rat cerebral slices. A fresh slice illuminated by near-vertical light was observed with a ...

    journal_title:Neuroscience research

    pub_type: 杂志文章


    authors: Hishida R,Hoshino K,Kudoh M,Norita M,Shibuki K

    更新日期:2003-06-01 00:00:00

  • Long lasting activity of nociceptive muscular afferents facilitates bilateral flexion reflex pattern in the feline spinal cord.

    abstract::Chronic muscular limb pain requires the adoption of motor patterns distinct from the classic ipsilateral flexion, crossed extension and corresponding reciprocal inhibitions to acute exteroceptive stimulation. Using selective chemical activation of group III/IV afferents in gastrocnemius-soleus (GS) muscles we investig...

    journal_title:Neuroscience research

    pub_type: 杂志文章


    authors: Schomburg ED,Steffens H,Pilyavskii AI,Maisky VA,Brück W,Dibaj P,Sears TA

    更新日期:2015-06-01 00:00:00

  • Spatial and dynamic changes in intracellular Ca2+ measured by confocal laser-scanning microscopy in bullfrog sympathetic ganglion cells.

    abstract::Confocal laser scanning microscopy (CLSM) was used to record spatial and dynamic changes in the intracellular Ca2+ [(Ca2+]i) of bullfrog sympathetic ganglion cells in excised tissue or in culture. A CLSM utilizing Ar ion laser (488 nm) and recording fluo-3 fluorescence yielded the sliced image of ganglion cells, while...

    journal_title:Neuroscience research

    pub_type: 杂志文章


    authors: Kuba K,Hua SY,Nohmi M

    更新日期:1991-05-01 00:00:00

  • Postsynaptic density visualized by whole mount electron microscopy.

    abstract::Detergent-unextractable structures of synaptic plasma membrane of rat cerebrum were observed by whole mount electron microscopy. The globular structures were identified as postsynaptic densities (PSDs) from several criteria and appeared to consist of folded strings, to which a number of other molecules might be attach...

    journal_title:Neuroscience research

    pub_type: 杂志文章


    authors: Suzuki T,Tanaka R

    更新日期:1986-02-01 00:00:00

  • Bilateral intrahippocampal NAC61-95 effects on behavior and moderation with L-NAME treatment.

    abstract::Residues 61-95 of the non-amyloid component (NAC(61-95)) domain of alpha-synuclein are responsible for the aggregation and neurotoxicity of this protein. This study evaluated the effect of N((omega))-nitro-l-arginine methyl ester (l-NAME), a nitric oxide synthase inhibitor, on the behavior of rats bilaterally injected...

    journal_title:Neuroscience research

    pub_type: 杂志文章


    authors: O'Hare E,Elliott JJ,Hobson P,O'Mara SM,Spanswick D,Kim EM

    更新日期:2010-02-01 00:00:00

  • Regional distribution of substance P, neurokinin alpha and neurokinin beta in rat central nervous system.

    abstract::Recently, we have found novel mammalian tachykinins, neurokinin alpha and beta isolated from porcine spinal cord, whose chemical structures are similar to those of substance P or kassinin. In the present study, we describe a specific assay system for neurokinin alpha and beta by combining HPLC and radioimmunoassay. We...

    journal_title:Neuroscience research

    pub_type: 杂志文章


    authors: Kanazawa I,Ogawa T,Kimura S,Munekata E

    更新日期:1984-12-01 00:00:00

  • A novel balanced chromosomal translocation found in subjects with schizophrenia and schizotypal personality disorder: altered l-serine level associated with disruption of PSAT1 gene expression.

    abstract::l-Serine is required for the synthesis of glycine and d-serine, both of which are NMDA receptor co-agonists. Although roles for d-serine and glycine have been suggested in schizophrenia, little is known about the role of the l-serine synthesizing cascade in schizophrenia or related psychiatric conditions. Here we repo...

    journal_title:Neuroscience research

    pub_type: 杂志文章


    authors: Ozeki Y,Pickard BS,Kano S,Malloy MP,Zeledon M,Sun DQ,Fujii K,Wakui K,Shirayama Y,Fukushima Y,Kunugi H,Hashimoto K,Muir WJ,Blackwood DH,Sawa A

    更新日期:2011-02-01 00:00:00

  • Oligosynaptic inhibition of group I afferents between the brachioradialis and flexor carpi radialis in humans.

    abstract::Spinal reflex arcs mediated by low threshold afferents between the brachioradialis (BR) and flexor carpi radialis (FCR) were studied in eleven healthy human subjects using a post-stimulus time-histogram method. Electrical conditioning stimuli (ES) to the radial nerve branch innervating BR with the intensity below the ...

    journal_title:Neuroscience research

    pub_type: 杂志文章


    authors: Kobayashi S,Hayashi M,Shinozaki K,Nito M,Hashizume W,Miyasaka T,Shindo M,Naito A

    更新日期:2016-09-01 00:00:00

  • The effect of intrastriatal single injection of GDNF on the nigrostriatal dopaminergic system in hemiparkinsonian rats: behavioral and histological studies using two different dosages.

    abstract::Glial cell line-derived neurotrophic factor (GDNF) is a member of the transforming growth factor-beta superfamily and acts as a neurotrophic factor for the nigrostriatal dopamine (DA) system. Although previous studies have shown that pretreatment with GDNF could prevent degenerative changes of nigrostriatal DA system ...

    journal_title:Neuroscience research

    pub_type: 杂志文章


    authors: Aoi M,Date I,Tomita S,Ohmoto T

    更新日期:2000-04-01 00:00:00

  • Comparative effects of implanted electrodes with differing contact patterns on peripheral nerve regeneration and functional recovery.

    abstract::Electrical stimulation could enhance nerve regeneration and functional recovery. The objective of this study was to evaluate the regenerative effects of implanted electrodes with different contacts in resected sciatic nerve. Sciatic nerve resection and microsurgical repair models were established and randomly divided ...

    journal_title:Neuroscience research

    pub_type: 杂志文章


    authors: Yu AP,Shen YJ,Qiu YQ,Li J,Shen YD,Wang XM,Cong M,He QR,Chen QZ,Jiang S

    更新日期:2019-08-01 00:00:00

  • Uni-directional differentiation of mouse embryonic stem cells into neurons by the neural stem sphere method.

    abstract::We previously showed that our neural stem sphere (NSS) method promotes the neuronal differentiation of mouse, monkey and human embryonic stem (ES) cells. Here we analyzed changes in expression of marker genes and proteins during neuronal differentiation. When cultured in astrocyte-conditioned medium (ACM) under free-f...

    journal_title:Neuroscience research

    pub_type: 杂志文章


    authors: Otsu M,Sai T,Nakayama T,Murakami K,Inoue N

    更新日期:2011-04-01 00:00:00

  • Calcium-binding protein, secretagogin, characterizes novel groups of interneurons in the rat striatum.

    abstract::In the rat striatum numerous secretagogin (SCGN) positive neurons were scattered. They were heterogeneous in their morphological and chemical properties. We examined the colocalization of SCGN with known four interneuron markers, parvalbumin (PV), calretinin (CR), nitric oxide synthase (NOS) and choline acetyl transfe...

    journal_title:Neuroscience research

    pub_type: 杂志文章


    authors: Kosaka T,Yasuda S,Kosaka K

    更新日期:2017-06-01 00:00:00

  • Regional and laminar distribution of choline acetyltransferase immunoreactivity in the cat superior colliculus.

    abstract::The pattern of distribution of cholinergic fibers was examined immunohistochemically in the cat superior colliculus by using a monoclonal antibody against choline acetyltransferase (ChAT). In the superficial layers, an obvious immunoreactive zone was found in the rostral two-thirds of the outer portion of the superfic...

    journal_title:Neuroscience research

    pub_type: 杂志文章


    authors: Hashikawa T

    更新日期:1989-06-01 00:00:00

  • A possible serologic biomarker for maternal immune activation-associated neurodevelopmental disorders found in the rat models.

    abstract::Epidemiological studies have shown that maternal infection during early pregnancy increases the risk of neurodevelopmental disorders (i.e., schizophrenia or autism) in offspring. Recently, diagnostic/stratification biomarkers for the maternal immune activation background in patients with neurodevelopmental disorders h...

    journal_title:Neuroscience research

    pub_type: 杂志文章


    authors: Oh-Nishi A,Koga K,Maeda T,Suhara T

    更新日期:2016-12-01 00:00:00

  • Diazepam restores the increased [3H]glutamate binding to hippocampal synaptic membranes in the amygdaloid-kindled rat.

    abstract::[3H]Glutamic acid binding to hippocampi was increased after amygdaloid kindling in rats, and diazepam inhibited this increased binding, without any effect on the enhanced binding by CaCl2 or the binding in control rats. By inducing kindling in the same way as that used in the binding experiment, the inhibiting effects...

    journal_title:Neuroscience research

    pub_type: 杂志文章


    authors: Oka J,Yamada M,Goto M,Fukuda H

    更新日期:1987-02-01 00:00:00

  • Enzymatic inactivation of tachykinin neurotransmitters in the isolated spinal cord of the newborn rat.

    abstract::A mixture of peptidase inhibitors increased the magnitude of the saphenous nerve-evoked slow depolarization of a lumbar ventral root and prolonged the similarly evoked inhibition of monosynaptic reflex (MSR) in the isolated spinal cord of the newborn rat in the presence of naloxone. The saphenous nerve-evoked MSR inhi...

    journal_title:Neuroscience research

    pub_type: 杂志文章


    authors: Yanagisawa M,Yoshioka K,Kurihara T,Saito K,Seno N,Suzuki H,Hosoki R,Otsuka M

    更新日期:1992-12-01 00:00:00

  • Brain development and multiple molecular species of proteoglycan.

    abstract::The occurrence of multiple proteoglycan species is a characteristic of the brain. The structural features of individually characterized proteoglycans in the brain are first introduced in brief, then some examples are shown that suggest a relationship between multiple proteoglycans and the many distinct cell types and ...

    journal_title:Neuroscience research

    pub_type: 杂志文章,评审


    authors: Oohira A,Katoh-Semba R,Watanabe E,Matsui F

    更新日期:1994-09-01 00:00:00

  • Zinc ion enhances the blocking potency of synthetic analogs of spider toxin (JSTX) on the glutamate receptor.

    abstract::The effect of synthetic spider toxin analogs containing aza-crown in combination with zinc and copper was studied on the lobster neuromuscular synapse. The suppressive action of N4-type spider toxin analog (N4) on excitatory postsynaptic potentials (EPSPs) was markedly enhanced in the presence of 10(-5)-10(-4) M Zn2+....

    journal_title:Neuroscience research

    pub_type: 杂志文章


    authors: Kawai N,Miwa A,Hashimoto Y,Shudo K,Asami T,Nakajima T

    更新日期:1989-04-01 00:00:00

  • Regulated transgene delivery by ganciclovir in the brain without physiological alterations by a live attenuated herpes simplex virus vector.

    abstract::The distribution of a live attenuated herpes simplex virus (betaH1)-mediated gene delivery into the central nervous system (CNS) was regulated by growth inhibition with ganciclovir (GCV) and the effect of this transgene expression system on the physiologic response was characterized by the acoustic startle response an...

    journal_title:Neuroscience research

    pub_type: 杂志文章


    authors: Fukuda Y,Yamamura J,Uwano T,Nishijo H,Kurokawa M,Fukuda M,Ono T,Shiraki K

    更新日期:2003-02-01 00:00:00

  • DISC1 regulates synaptic vesicle transport via a lithium-sensitive pathway.

    abstract::Disrupted-in-Schizophrenia 1 (DISC1) is a susceptibility gene for major mental illnesses, including bipolar disorder and schizophrenia. Although the roles of DISC1 in nervous system development and functions are increasingly recognized, pathophysiological mechanisms underlying a range of neuropsychiatric symptoms caus...

    journal_title:Neuroscience research

    pub_type: 杂志文章


    authors: Flores R 3rd,Hirota Y,Armstrong B,Sawa A,Tomoda T

    更新日期:2011-09-01 00:00:00

  • Angiotensin II type-2 (AT2) receptor antagonism alters cardiovascular responses to static exercise and simultaneously changes glutamate/GABA levels within the ventrolateral medulla.

    abstract:UNLABELLED:Angiotensin II receptors (Ang II), classified into AT1 and AT2 subtypes, are located in different regions of the central nervous system, including the cardiovascular control centers in the medulla oblongata. We previously reported the role of Ang II AT1 receptors within the medulla on cardiovascular response...

    journal_title:Neuroscience research

    pub_type: 杂志文章


    authors: Tedesco A,Ally A

    更新日期:2009-08-01 00:00:00

  • Nitric oxide, 2-amino-4-phosphonobutyric acid and light/dark adaptation modulate short-wavelength-sensitive synaptic transmission to retinal horizontal cells.

    abstract::Light-induced changes in the input resistance (Rin) of external, luminosity (i.e. H1) type horizontal cell (HC) perikarya were studied by the bridge-balance method in light-adapted and dark-adapted retinae of carp. Changes in input resistance (delta Rin) induced by short-(460 nm) and long-wavelength (674 nm) flashes, ...

    journal_title:Neuroscience research

    pub_type: 杂志文章


    authors: Furukawa T,Yamada M,Petruv R,Djamgoz MB,Yasui S

    更新日期:1997-01-01 00:00:00

  • Involvement of oxidative stress in the enhancement of acetylcholinesterase activity induced by amyloid beta-peptide.

    abstract::Acetylcholinesterase (AChE) activity is increased within and around amyloid plaques, which are present in Alzheimer's disease (AD) patient's brain. In this study, using cultured retinal cells as a neuronal model, we analyzed the effect of the synthetic peptide Abeta(25-35) on the activity of AChE, the degradation enzy...

    journal_title:Neuroscience research

    pub_type: 杂志文章


    authors: Melo JB,Agostinho P,Oliveira CR

    更新日期:2003-01-01 00:00:00

  • Quantitative and temporal relationship between local cerebral blood flow and neuronal activation induced by somatosensory stimulation in rats.

    abstract::In many studies on functional neuroimaging, change in local cerebral blood flow induced by sensory stimulation (evoked LCBF) is used as a marker for change in cortical neuronal activity, although a full description of the relationship between the evoked LCBF and neuronal activity has not been given. The purpose of thi...

    journal_title:Neuroscience research

    pub_type: 杂志文章


    authors: Matsuura T,Kanno I

    更新日期:2001-07-01 00:00:00