Abstract:
AIM:Ultraviolet (UV) B irradiation induces gene expression that leads to skin cancer. Among the transcription factors induced by UVB radiation exposure, the cyclic AMP response element-binding protein (CREB) is significant. Since several factors downstream of CREB signaling are known to be involved in pterygium pathogenesis, we investigated CREB expression in pterygial and human conjunctival tissues to evaluate if a similar expression pattern is present. Moreover, we analyzed the correlation with CREB expression and other known pterygium markers. METHODS:Primary pterygium samples and normal bulbar conjunctivas surgically removed were analyzed. Formalin-fixed, paraffin-embedded tissues were stained by immunohistochemistry with anti-CREB, anti-vimentin, anti-ki-67, anti-survivin, anti-MMP7, anti-p63, anti-cyclin D1, or anti-p53 antibodies. RESULTS:94.4% of pterygium samples were positive for CREB with a significant difference compared to the control group (p = 0.002). The staining was localized in the epithelium and absent in the stroma. An increased expression was found for cyclin D1 (p = 0.019), ki-67 (p = 0.005), vimentin (p = 0.003), survivin (p < 0.001), p63 (p = 0.003), and MMP7 (p = 0.002). CREB expression showed a significant correlation with cyclin D1 (ρ = 0.49; p = 0.035), ki-67 (ρ = 0.61; p = 0.007), and p53 (ρ = 0.57; p = 0.013) in pterygium. CONCLUSIONS:These results permit to hypothesize that CREB is involved in pterygium pathogenesis. Since various molecules have been discovered to inhibit CREB, these data could be of interest for pterygium treatment.
journal_name
Ophthalmic Resjournal_title
Ophthalmic researchauthors
Nubile M,Curcio C,Lanzini M,Calienno R,Iezzi M,Mastropasqua A,Di Nicola M,Mastropasqua Ldoi
10.1159/000347124subject
Has Abstractpub_date
2013-01-01 00:00:00pages
99-107issue
2eissn
0030-3747issn
1423-0259pii
000347124journal_volume
50pub_type
杂志文章abstract:PURPOSE:The aim of this study was to evaluate the characteristics of corneal endothelial cells and central corneal thickness (CCT) in patients with diabetes mellitus (DM), comparing them with those of healthy subjects (controls) and to determine potential factors affecting the corneal parameters in patients with DM. M...
journal_title:Ophthalmic research
pub_type: 杂志文章
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更新日期:2020-01-01 00:00:00
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journal_title:Ophthalmic research
pub_type: 临床试验,杂志文章,随机对照试验
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abstract::Diabetic macular edema is a common condition frequently encountered by ophthalmologists. In this piece we briefly review its definition, pathogenesis, clinical aspects, imaging, and treatment. ...
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pub_type: 杂志文章,评审
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更新日期:2014-01-01 00:00:00
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abstract::To study the mode of riboflavin metabolism in the lens in vivo, the uptake of 14C-labeled riboflavin and synthesis of ester forms from 14C-riboflavin were assayed in the single whole lens of the rats. 14C-riboflavin uptake increased in proportion to the substrate concentration in the incubation medium, reaching a plat...
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journal_title:Ophthalmic research
pub_type: 杂志文章
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abstract::The ophthalmic examination of patients with hypertrophic cardiomyopathy treated with high doses of verapamil over a considerable period of time showed no evidence of a side effect of the drug, thus proving its ocular safety. This could be verified by densitometric image analysis of Scheimpflug photographs of the eyes....
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journal_title:Ophthalmic research
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更新日期:2017-01-01 00:00:00
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更新日期:2014-01-01 00:00:00
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journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000267707
更新日期:1995-01-01 00:00:00
abstract::Tear hyperosmolarity is known to cause ocular surface inflammation in dry eye syndrome. Benzalkonium chloride (BAK), an eyedrop preservative, is known to induce dry eye in long-term-treated patients. Analyzing the modulation of the proinflammatory potential of hyperosmolarity in the presence of BAK on the conjunctiva ...
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更新日期:2017-01-01 00:00:00
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更新日期:2008-01-01 00:00:00
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更新日期:2018-01-01 00:00:00
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journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000265400
更新日期:1985-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2010-01-01 00:00:00
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journal_title:Ophthalmic research
pub_type: 杂志文章
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更新日期:1990-01-01 00:00:00
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journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000267497
更新日期:1994-01-01 00:00:00
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journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000065600
更新日期:2002-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2020-01-01 00:00:00
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pub_type: 杂志文章,评审
doi:10.1159/000489093
更新日期:2018-01-01 00:00:00