Abstract：
AIMS:To evaluate the biologic effects of fluoroquinolone eye solutions without preservatives on cultured human corneal epithelial cells in vitro. METHODS:We studied the effect of diverse generations of topical fluoroquinolones such as ofloxacin 0.3%, levofloxacin 0.5%, tosufloxacin 0.3%, moxifloxacin 0.5% and gatifloxacin 0.3% on cultured human corneal epithelial cells. MTT-based calorimetric assay, lactate dehydrogenase leakage (LDH) assay and scratch wound test were performed. Corneal epithelial cell morphologies were examined by performing inverted light microscopy and transmission electron microscopy. RESULTS:In all topical fluoroquinolones, the metabolic activity of the corneal epithelial cells decreased in a time-dependent fashion and the LDH titer increased with longer exposure times. Especially, the LDH titers significantly increased after exposure to moxifloxacin 0.5% and gatifloxacin 0.3% compared with controls. The migration rates of the corneal epithelial cells were faster in ofloxacin 0.3% or levofloxacin 0.5% than other fluoroquinolones. Severe cellular morphological damage was observed after exposure to moxifloxacin 0.5% and gatifloxacin 0.3%. CONCLUSIONS:As moxifloxacin 0.5% and gatifloxacin 0.3% induced the toxic effect to the corneal epithelial cells, compared with other fluoroquinolones, the 4th fluoroquinolone eye solutions should be carefully used in case of the corneal epithelium is damaged by long duration of treatment or overdosage.
journal_name
Ophthalmic Resjournal_title
Ophthalmic researchauthors
Oum BS,Kim NM,Lee JS,Park YMdoi
10.1159/000357976subject
Has Abstractpub_date
2014-01-01 00:00:00pages
216-23issue
4eissn
0030-3747issn
1423-0259pii
000357976journal_volume
51pub_type
杂志文章abstract：PURPOSE:To characterize the 1-year progression of retinal thickness (RT) increase occurring in eyes with subclinical macular edema in type 2 diabetes. METHODS:Forty-eight type 2 diabetic eyes/patients with mild nonproliferative diabetic retinopathy (NPDR; levels 20 and 35 in the Early Treatment Diabetic Retinopathy St...
journal_title：Ophthalmic research
pub_type： 杂志文章,多中心研究
doi：10.1159/000438793
更新日期：2015-01-01 00:00:00
abstract：:The aim of the present study was to investigate biochemical and morphological changes in rat lenses following long-term UV B irradiation. After an irradiation period of 156 days with follow-up documentation by means of Scheimpflug photography, section-related biochemical analyses of the lenses as well as histological ...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000267187
更新日期：1992-01-01 00:00:00
abstract：:The topographic distribution of enzyme activities in normal rat lenses and their changes occurring during naphthalene cataract development were investigated. Cataract formation was documented by slit images according to the Scheimpflug principle. Before the onset of visible changes and after the development of early v...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000267150
更新日期：1992-01-01 00:00:00
abstract：AIMS:Staphylococcus epidermidis is considered a commensal bacterium; however, it is frequently isolated from ocular infections showing a multidrug resistance. Ciprofloxacin-resistant strains have been isolated from ocular infections; however, resistance to quinolone, such as gatifloxacin and moxifloxacin, is not often ...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000219684
更新日期：2009-01-01 00:00:00
abstract：:HY-1 genotype chick lenses display epithelial hyperplasia and associated lens opacities. Membrane proteins from HY-1 and normal chick lenses were separated by isoelectric focusing, and 45Ca2+ binding capacities were determined. Markedly higher (congruent to 900-fold) binding of 45Ca2+ was observed in high molecular we...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000265397
更新日期：1985-01-01 00:00:00
abstract：PURPOSE:Osthole is an agent isolated from Cnidium monnieri (L.) Cusson and has been used to treat several disorders. Corneal neovascularization is a sight-threatening condition associated with several inflammatory or infectious ocular disorders. In this study, we investigated the anti-angiogenic effects of osthole on c...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000505976
更新日期：2020-01-01 00:00:00
abstract：:The specific activities of the four investigated enzymes of the carbohydrate metabolism decrease with ageing. In the old tissue, phosphoglyceratemutase and enolase sustain changes in their molecular weight, and their substrate affinity is modified. ...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000265275
更新日期：1983-01-01 00:00:00
abstract：PURPOSE:To investigate the effects, and their reversibility, of multiple oral voriconazole doses on a variety of visual tests in healthy male volunteers. METHODS:Single-center, double-blind, randomized, placebo-controlled, parallel-group study in 36 volunteers who received voriconazole (n=18, 400 mg every 12 h on day ...
journal_title：Ophthalmic research
pub_type： 杂志文章,随机对照试验
doi：10.1159/000359952
更新日期：2014-01-01 00:00:00
abstract：:The vibrating probe detects and measures currents around cells and organs. With its use, it is possible to demonstrate strong outward potassium currents at the equator of the lens and strong inward sodium currents at the optical poles. The addition of microelectrodes to the system permits one to define the nature of t...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000266570
更新日期：1988-01-01 00:00:00
abstract：PURPOSE:To investigate the role of integrin-linked kinase (ILK) in endothelial cell migration, proliferation and tube formation in vitro. MATERIALS AND METHODS:Cultured RF/6A cells were knocked down for ILK using small interfering RNA (siRNA). Cellular ILK expression was quantified by real-time quantitative PCR and We...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000232971
更新日期：2009-01-01 00:00:00
abstract：BACKGROUND:Diabetic retinopathy (DR) is a severe ocular complication of diabetes. Kallistatin has multiple biological functions including anti-inflammation and antiangiogenesis. Our aim was to detect the level of kallistatin in the vitreous of proliferative DR (PDR) and its effect on proliferation, migration, and tube ...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000447776
更新日期：2017-01-01 00:00:00
abstract：:Autofluorescence and transmission values of the lens for blue-green light were determined at two locations with the use of identical fluorophotometers: in Coimbra, Portugal, in 44 healthy controls, 15 haemodialysis and 11 renal failure patients, and in Leiden, The Netherlands, in 57 healthy controls, 23 haemodialysis ...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000266736
更新日期：1988-01-01 00:00:00
abstract：:The refractive indices in various rat retinal layers, based on the total dry mass concentration, ranged from 1.369 to 1.385. The ratios of the refractive indices of neighboring retinal layers were near unity. This finding suggests that light passes through the rat retina without much refraction. ...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000267223
更新日期：1993-01-01 00:00:00
abstract：:Ocular pneumoplethysmography was performed before and after 1,737 carotid endarterectomies, 82% of which were performed for carotid lesions of hemodynamic consequence. Preoperative ocular hypoperfusion without ischemia was associated with at least transient postoperative ocular hyperemia. A difference in ocular volume...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000055558
更新日期：1999-01-01 00:00:00
abstract：BACKGROUND:Myopia has become a worldwide public health issue, which is occurring at a younger age, leading to an increased risk of high myopia. Ocular complications associated with high myopia can lead to irreversible vision loss. It is urgent and critical to explore effective treatment to slow or even stop the progres...
journal_title：Ophthalmic research
pub_type： meta分析
doi：10.1159/000510779
更新日期：2020-08-11 00:00:00
abstract：BACKGROUND/AIMS:By the release of antiangiogenic factors, Müller glial cells provide an angiostatic environment in the normal and ischemic retina. We determined whether Müller cells produce thrombospondin-1 (TSP-1), a known inhibitor of angiogenesis. METHODS:Secretion of TSP-1 by cultured Müller cells was determined w...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000362371
更新日期：2014-01-01 00:00:00
abstract：PURPOSE:To evaluate the corneal button of primary penetrating keratoplasty of patients diagnosed with congenital aniridia. The study took place at the Instituto Universitario Barraquer and the Centro de Oftalmología Barraquer. METHODS:A retrospective analysis of cases diagnosed with congenital aniridia was carried out...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000444930
更新日期：2016-01-01 00:00:00
abstract：:Soluble dextran polymer derivatives (CMDBSs) are originally synthesized as heparin-like plasma substitutes. Some of them mimic heparin in its interactions and stabilize, protect and facilitate actions of heparin binding growth factors. The wound healing activity of one specific CMDBS was studied in a model of corneal ...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000267497
更新日期：1994-01-01 00:00:00
abstract：:Oxidative stress plays a role in the pathogenesis of neurodegeneration in diabetic retinopathy (DR). However, whether an excellent reactive oxygen species scavenger, U83836E, inhibits the neurodegeneration in DR remains uncertain. This study is aimed at clarifying the effects of U83836E on DR. Two weeks after streptoz...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000321952
更新日期：2011-01-01 00:00:00
abstract：:In corneal organ culture, a contamination of sterile culture media with endotoxin is frequently found. Thus, we investigated if the presence of endotoxin affects the viability of cultured porcine corneal endothelial cells. Endotoxin in high concentrations caused morphological cell changes in porcine corneal endothelia...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000055566
更新日期：1999-01-01 00:00:00
abstract：PURPOSE:The purpose of this study was to evaluate the anatomical and functional findings in patients with vitreomacular traction (VMT) combined with epiretinal membrane (ERM) in the same eye. METHODS:In this retrospective, cross-sectional study, we studied 65 patients with VMT and ERM. In 36 of them, ERM/VMT had a 'un...
journal_title：Ophthalmic research
pub_type： 杂志文章,多中心研究
doi：10.1159/000446658
更新日期：2017-01-01 00:00:00
abstract：:The pathology of rainbow trout lenses incubated in various media and at various temperatures was examined by electron microscopy. After incubation at 0 degrees C for 5 h, no changes were observed in transparency and cytology. Irreversible warm cataract occurred at 37 degrees C, which corresponds to the body temperatur...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000265427
更新日期：1986-01-01 00:00:00
abstract：BACKGROUND:We assessed bleb morphology and the intraocular pressure (IOP)-lowering effect of trabeculectomy with ologen compared to mitomycin C (MMC) in juvenile open-angle glaucoma (JOAG). METHODS:This is a prospective interventional comparative study conducted on 40 eyes (20 patients) with medically uncontrolled JOA...
journal_title：Ophthalmic research
pub_type： 杂志文章,随机对照试验
doi：10.1159/000456719
更新日期：2017-01-01 00:00:00
abstract：:To determine the effect of interleukin-10 (IL-10) and transforming growth factor-beta (TGF-beta) on the down-regulation of MHC class II antigens, bovine retinal pigment epithelial (RPE) cells were incubated with interferon-gamma (IFN-gamma) in different concentrations. Subsequently, the IFN-gamma pretreated RPE cells ...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000055545
更新日期：1999-01-01 00:00:00
abstract：:Modulation sensitivity functions ( MSFs ) of retinal ganglion cells of the pigeon were determined by recording action potentials from their end branching in the optic tectum as response indicator. The inverse Fourier transformation of a MSF is taken to represent the receptive field sensitivity function approximating t...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000265299
更新日期：1984-01-01 00:00:00
abstract：:The purpose of this study was to compare the variability of the amplitude ratios and latencies of the electro-oculogram (EOG) light peak (LP) in three different recording conditions: Arden ratio (dark trough (DT): 12 min), modified Arden ratio (DT: 15 min) and light peak/dark baseline ratio (LP/BL: 35 min of dark adap...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000267320
更新日期：1993-01-01 00:00:00
abstract：AIMS:To investigate whether allogeneic limbal mesenchymal stem cell (LMSC) therapy affects corneal healing after a severe chemical burn and whether the route of administration of LMSCs differs in its therapeutic effect in this respect. METHODS:A total of 60 Sprague-Dawley rats with clinically proven alkali injury were...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000368659
更新日期：2015-01-01 00:00:00
abstract：PURPOSE:To investigate the effect of propolis, a natural resin produced by honeybees, in the treatment of alkali-injured rabbit eyes. METHOD:A corneal alkali burn was induced by applying filter paper immersed in 1 N NaOH on the central axis of the right corneas of 24 rabbits for 30 s. The animals were divided into thr...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000055581
更新日期：2000-01-01 00:00:00
abstract：:We examined 304 conjunctiva in 152 subjects by clinical, cytologic, and bacteriologic methods. Bacteriologic samples were evaluated after a 48-hour incubation. Bacterial growth was found in 8 (20.5%) of 39 patients with clinically normal conjunctiva and no polymorphonuclear leukocytes (PMNs), and in 8 (61.5%) of 13 su...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000267086
更新日期：1991-01-01 00:00:00
abstract：:To ascertain the effectiveness of topical antioxidant therapy on acute corneal inflammation, we have studied the effectiveness of topical treatment with a saline solution and with antioxidants such as 0.2% superoxide dismutase and 0.5% dimethylthiourea (DMTU) in a controlled experimental study. The evolution of the in...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000267648
更新日期：1995-01-01 00:00:00
