Abstract:
:It is well known that physical inactivity leads to loss of muscle mass, but it also causes bone loss. Mechanistically, osteoclastogenesis and bone resorption have recently been shown to be regulated by vibration. However, the underlying mechanism behind the inhibition of osteoclast formation is yet unknown. Therefore, we investigated whether mechanical vibration of osteoclast precursor cells affects osteoclast formation by the involvement of fusion-related molecules such as dendritic cell-specific transmembrane protein (DC-STAMP) and P2X7 receptor (P2X7R). RAW264.7 (a murine osteoclastic-like cell line) cells were treated with 20ng/ml receptor activator of NF-κB ligand (RANKL). For 3 consecutive days, the cells were subjected to 1h of mechanical vibration with 20μm displacement at a frequency of 4Hz and compared to the control cells that were treated under the same condition but without the vibration. After 5days of culture, osteoclast formation was determined. Gene expression of DC-STAMP and P2X7R by RAW264.7 cells was determined after 1h of mechanical vibration, while protein production of the DC-STAMP was determined after 6h of postincubation after vibration. As a result, mechanical vibration of RAW264.7 cells inhibited the formation of osteoclasts. Vibration down-regulated DC-STAMP gene expression by 1.6-fold in the presence of RANKL and by 1.4-fold in the absence of RANKL. Additionally, DC-STAMP protein production was also down-regulated by 1.4-fold in the presence of RANKL and by 1.2-fold in the absence of RANKL in RAW264.7 cells in response to mechanical vibration. However, vibration did not affect P2X7R gene expression. Mouse anti-DC-STAMP antibody inhibited osteoclast formation in the absence of vibration. Our results suggest that mechanical vibration of osteoclast precursor cells reduces DC-STAMP expression in osteoclast precursor cells leading to the inhibition of osteoclast formation.
journal_name
Bonejournal_title
Boneauthors
Kulkarni RN,Voglewede PA,Liu Ddoi
10.1016/j.bone.2013.08.020subject
Has Abstractpub_date
2013-12-01 00:00:00pages
493-8issue
2eissn
8756-3282issn
1873-2763pii
S8756-3282(13)00335-9journal_volume
57pub_type
杂志文章相关文献
BONE文献大全abstract::Saracatinib (AZD0530) is an orally active once-daily Src kinase inhibitor which modulates key signaling pathways in cancer cells. In a Phase I study in patients with advanced solid malignancies resistant to standard treatment we assessed the effect of saracatinib on bone turnover. Fifty-one patients were randomized in...
journal_title:Bone
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1016/j.bone.2011.12.017
更新日期:2012-04-01 00:00:00
abstract::The reversible calcitonin (CT)-induced inhibition of osteoclastic activity has been studied to clarify the mechanisms responsible for the so-called "escape phenomenon." Osteoclasts disaggregated from neonatal rabbits were cultured on glass coverslips or thin bovine bone slices. Resorption activity was evaluated by usi...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/8756-3282(89)90010-0
更新日期:1989-01-01 00:00:00
abstract::In patients with osteoporosis, alendronate treatment causes an increase in bone mineral density (BMD) and a decrease in fracture incidence. Alendronate acts by changing the bone remodeling process. Changes in bone remodeling resulting in decreased remodeling space, increased bone balance per remodeling cycle, and incr...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(01)00613-5
更新日期:2001-12-01 00:00:00
abstract::High-resolution peripheral quantitative computed tomography (HR-pQCT) assesses both volumetric bone mineral density (vBMD) and trabecular and cortical microarchitecture. However, studies of the association of HR-pQCT parameters with fracture history have been small, predominantly limited to postmenopausal women, often...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2018.05.005
更新日期:2018-08-01 00:00:00
abstract::Increases in local and systemic bone resorption are hallmarks of rheumatoid arthritis (RA). Osteoclasts are implicated in these processes and their enhanced differentiation may contribute to bone destruction. We observed that in vitro osteoclastogenesis varies among healthy individuals and hypothesized that increased ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2010.10.167
更新日期:2011-03-01 00:00:00
abstract::Progressive osseous heteroplasia (POH) is a rare autosomal dominant disorder of mesenchymal differentiation characterized by progressive heterotopic ossification (HO) of dermis, deep connective tissues and skeletal muscle. Usually, initial bone formation occurs during infancy as primary osteoma cutis (OC) then progres...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2013.06.015
更新日期:2013-10-01 00:00:00
abstract:INTRODUCTION:Osteocalcin (OC) is the most abundant non-collagenous bone protein and is determinant for bone mineralization. We aimed to compare OC bone expression and serum factors related to its carboxylation in hip fragility fracture and osteoarthritis patients. We also aimed to identify which of these factors were a...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2012.08.129
更新日期:2012-12-01 00:00:00
abstract::The combination of PTH with OPG has been proposed as a potential therapy in patients with severe osteoporosis. In the present study, we examined the bone material of aged ovariectomized (OVX) rats treated either with PTH (1-34) or OPG alone or in combination of both. The micro- and nanostructural characteristics of th...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2005.03.013
更新日期:2005-07-01 00:00:00
abstract::Metastatic bone disease has a major impact on the morbidity and mortality of breast cancer patients, and studies on bone metastasis biology have led to the development of the most widely used drugs for bone metastases treatment: zoledronate (Zol) and denosumab (Den). The aim of the present study was to assess the effe...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2014.06.017
更新日期:2014-09-01 00:00:00
abstract::Sclerostin, product of the SOST gene, is an important determinant of bone formation and resorption. Adolescents with anorexia nervosa (AN) have low bone density and decreased levels of bone turnover markers. However, sclerostin has not been examined in AN as a potential mediator of impaired bone metabolism. Our study ...
journal_title:Bone
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.bone.2012.06.006
更新日期:2012-09-01 00:00:00
abstract::One of the side effects associated with glucocorticoid therapy is glucocorticoid-induced bone loss. Glucocorticoids partly detain bone formation via the inhibition of osteoblastic function, however, the exact mechanism of this inhibition remains elusive. In this study, we examined the effect of dexamethasone, an activ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2011.01.017
更新日期:2011-05-01 00:00:00
abstract::Endochondral ossification in the growth plate of long bones involves cartilage mineralization, bone formation and the budding vasculature. Many of these processes take place in a complex and dynamic zone, the provisional ossification zone, of the growth plate. Here we investigate aspects of mineralization in 2D and 3D...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2019.115086
更新日期:2020-01-01 00:00:00
abstract::Bone morphogenetic protein 2 (BMP-2) is a member of the transforming growth factor-β superfamily, it is known to be a factor involved in skeletal development and capable of inducing in vitro osteogenic differentiation of mesenchymal stem cells (MSCs). Dental pulp stem cells (DPSCs) isolated from extracted third molar ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2019.115214
更新日期:2020-03-01 00:00:00
abstract::This study describes the early interface reaction of cancellous bone to a nanocrystalline hydroxyapatite cement containing type I collagen (HA/Coll) and its modifications with sodium citrate (CI), calcium carbonate (CA), phosphoserine (P) and phosphoserine plus RGD-peptide (RGD). Cylindrical implants of HA/Coll and it...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2006.11.019
更新日期:2007-04-01 00:00:00
abstract::Bone fragility is a complication of chronic kidney disease (CKD). Patients on dialysis have higher risk of fracture than the general population, but the reason remains obscure. Bone strength is determined by bone mass and bone quality. Although factors affecting bone quality include microarchitecture, remodeling activ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2011.03.672
更新日期:2011-06-01 00:00:00
abstract::How estrogen therapy influences bone metabolism in postmenopausal women has previously been studied using several approaches, including bone densitometry, measurement of biochemical markers of bone turnover, and histomorphometry. Taken together, these investigations suggest that conventional doses of estrogen protect ...
journal_title:Bone
pub_type: 杂志文章,评审
doi:10.1016/s8756-3282(98)00156-2
更新日期:1999-02-01 00:00:00
abstract::Previously we observed that capsaicin treatment in rats inhibited sensory neuropeptide signaling, with a concurrent reduction in trabecular bone formation and bone volume, and an increase in osteoclast numbers and bone resorption. Calcitonin-gene-related peptide (CGRP) is a neuropeptide richly distributed in sensory n...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2009.11.029
更新日期:2010-05-01 00:00:00
abstract::Aldehyde dehydrogenase 2 (ALDH2) is the enzyme that oxidizes the acetaldehyde produced by alcohol metabolism. This variant not only affects the response to alcohol but is also associated with several diseases, such as esophageal cancer, myocardial infarction, and particularly osteoporosis. In our previous study, we re...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2020.115370
更新日期:2020-07-01 00:00:00
abstract::Impaired osteoblast and osteocyte maturation contribute to mineralization defects and excess FGF23 expression in CKD bone. Vitamin D sterols decrease osteoid accumulation and increase FGF23 expression; these agents also increase osteoblast maturation in vitro but a link between changes in bone cell maturation, bone mi...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2019.07.026
更新日期:2019-10-01 00:00:00
abstract::Recent studies suggest that whole-body vibration (WBV) can improve measures of bone health for certain clinical conditions and ages. In the elderly, there also is particular interest in assessing the ability of physical interventions such as WBV to improve coordination, strength, and movement speed, which help prevent...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2010.07.014
更新日期:2010-10-01 00:00:00
abstract:CASE REPORT:A female patient with consanguineous parents presented with severe symptomatic hypocalcemia (1.62mmol/l) at the age of 4 months. Treatment with oral 1,25-(OH)2-vitamin D and calcium carbonate was started and serum calcium concentrations were stabilized at the lower end of the normal range. Subsequently she ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2012.06.009
更新日期:2012-09-01 00:00:00
abstract::Low body weight is associated with an increased risk for osteoporosis and fractures, but the contribution of other lifestyle related factors have not been previously studied within lean elderly women. The present study evaluated the association between lifelong lifestyle factors and bone density, falls and postmenopau...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2006.01.143
更新日期:2006-08-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Chronic kidney disease (CKD) is commonly associated with mineral and bone metabolism disorders, but these are less frequently studied in non-dialysis CKD patients than in dialysis patients. We examined and described international variation in mineral and bone disease (MBD) markers and their tr...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2019.115058
更新日期:2019-12-01 00:00:00
abstract::Administration of perioperative antibiotic prophylaxis is a routine procedure in orthopedic surgery. Besides systemic prophylaxis, only few techniques are established for local application of antibiotics to reduce infection related to orthopedic implants. The aim of this study was to evaluate the efficacy of locally v...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2005.01.008
更新日期:2005-05-01 00:00:00
abstract::Juvenile osteoporosis (JO) is characterized by bone fragility during development, low bone mass and absence of extraskeletal features. Heterozygous loss-of-function mutations in LRP5 have been found in a few patients, but bone tissue and bone material abnormalities associated with such mutations have not been determin...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2013.07.020
更新日期:2013-11-01 00:00:00
abstract::Osteocyte apoptosis precedes osteoclast resorption, and may act as a critical signal to trigger bone remodeling. While osteoclast precursors are known to travel via the circulation, the specific mechanisms by which they accumulate at remodeling sites are unclear. We hypothesized that osteocyte apoptosis mediates osteo...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2011.09.052
更新日期:2012-01-01 00:00:00
abstract:PURPOSE:Diabetes and osteoporosis occur frequently in older adults and are both associated with increased fracture risk. Denosumab treatment reduced new vertebral, nonvertebral, and hip fractures over 3 years, with continued low fracture incidence for up to 10 years in postmenopausal women with osteoporosis. However, i...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2020.115268
更新日期:2020-05-01 00:00:00
abstract::Twenty patients with mild Paget's disease of bone were given either 20 (10 patients) or 40 mg alendronate daily for 6 months. The 20-mg dose was well tolerated, but in 3 patients on 40 mg/d alendronate, the treatment was withdrawn after 3-5 months because of gastric and oesophageal disturbances. Urinary hydroxyproline...
journal_title:Bone
pub_type: 临床试验,杂志文章
doi:10.1016/8756-3282(94)90818-4
更新日期:1994-07-01 00:00:00
abstract::To define structure-activity relationships for bisphosphonate activity, we examined the bone binding and antiresorptive properties of heterocycle-containing analogs of risedronate, a pyridylbisphosphonate, in cultures of mouse fetal bone explants. Our studies indicated that hydroxybisphosphonates with the nitrogen mol...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(98)00120-3
更新日期:1998-11-01 00:00:00
abstract::Chronic inflammatory diseases are associated with bone loss. While the occurrence of systemic bone loss is well described in chronic inflammatory diseases, the impact of these conditions on articular bone has not been systematically investigated. Recent refinements in high-resolution CT assessment of the joints now al...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2018.07.017
更新日期:2018-11-01 00:00:00