Abstract:
:Osteocyte apoptosis precedes osteoclast resorption, and may act as a critical signal to trigger bone remodeling. While osteoclast precursors are known to travel via the circulation, the specific mechanisms by which they accumulate at remodeling sites are unclear. We hypothesized that osteocyte apoptosis mediates osteoclast precursor adhesion to vascular endothelium by regulating osteocytic secretion of IL-6 and soluble IL-6 receptor (sIL-6R) to promote endothelial ICAM-1 expression. We found that conditioned media from TNF-α-induced apoptotic MLO-Y4 osteocytes promoted RAW264.7 osteoclast precursor adhesion onto D4T endothelial cells (P<0.05). Blocking osteocyte apoptosis with a pan-caspase inhibitor (ZVAD-FMK) reduced osteoclast precursor adhesion to baseline levels (P<0.001). Endothelial cells treated with apoptotic osteocyte conditioned media had elevated surface expression of ICAM-1 (P<0.05), and blocking ICAM-1 abolished apoptosis-induced osteoclast precursor adhesion. Apoptotic osteocyte conditioned media contained more IL-6 (P<0.05) and sIL-6R (P<0.05) than non-apoptotic osteocyte conditioned media. When added exogenously, both IL-6 and sIL-6R were required for endothelial activation, and blocking IL-6 reduced apoptosis-induced osteoclast precursor adhesion to baseline levels (P<0.05). Therefore, we conclude that osteocyte apoptosis can promote osteoclast precursor adhesion to endothelial cells via ICAM-1; this is likely through increased osteocytic IL-6 and sIL-6R secretion, both of which are indispensible to endothelial activation.
journal_name
Bonejournal_title
Boneauthors
Cheung WY,Simmons CA,You Ldoi
10.1016/j.bone.2011.09.052subject
Has Abstractpub_date
2012-01-01 00:00:00pages
104-10issue
1eissn
8756-3282issn
1873-2763pii
S8756-3282(11)01272-5journal_volume
50pub_type
杂志文章相关文献
BONE文献大全abstract::Previously we observed that capsaicin treatment in rats inhibited sensory neuropeptide signaling, with a concurrent reduction in trabecular bone formation and bone volume, and an increase in osteoclast numbers and bone resorption. Calcitonin-gene-related peptide (CGRP) is a neuropeptide richly distributed in sensory n...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2009.11.029
更新日期:2010-05-01 00:00:00
abstract::Type 1 diabetes mellitus (T1DM) has been associated with deterioration of bone microarchitecture and strength, resulting in increased fracture risk. Substantial studies have revealed the capacity of moderate-intensity static magnetic fields (SMF) on promoting osteoblastogenesis in vitro and stimulating bone growth and...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2017.10.024
更新日期:2018-02-01 00:00:00
abstract::Bone geometry plays a prominent role in bone strength. Cross-sectional studies have shown that advancing age is associated with increasing diameter of long bones, related to both periostal apposition and endosteal resorption. However, there are few data provided by prospective studies, especially concerning the change...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2010.03.020
更新日期:2010-08-01 00:00:00
abstract::Human vertebral cancellous bone from white males (N = 19), black males (N = 16), white females (N = 12), and black females (N = 17) was examined histologically for the presence, numerical density, and morphology of in vivo microscopic cracking (microdamage). Two patterns of microcracks, linear and cross-hatched, were ...
journal_title:Bone
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/8756-3282(96)88871-5
更新日期:1996-08-01 00:00:00
abstract::The objects of this study were to determine the responses of a cancellous bone site with a closed growth plate (the distal tibial metaphysis, DTM) to ovariectomy (OVX) and OVX plus a prostaglandin E2 (PGE2) treatment, and compare the site's response to previous findings reported for another site (the proximal tibial m...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/8756-3282(94)90700-5
更新日期:1994-03-01 00:00:00
abstract::Levels of circulating cytokines are elevated in inflammatory diseases. Previously, it was shown that interleukin (IL-)17A, in synergism with 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] and tumor necrosis factor α (TNFα), induces the release of granulocyte-macrophage colony-stimulating factor (GM-CSF) by murine osteoblasts ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2017.01.036
更新日期:2017-04-01 00:00:00
abstract::Glucocorticoids have marked effects on bone metabolism, and continued exposure of skeletal tissue to excessive amounts of these steroids results in osteoporosis. Therefore, in the present proteomic study, we characterized the potential effects of glucocorticoids on protein expression in human osteoblastic cells. Using...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2003.09.010
更新日期:2004-02-01 00:00:00
abstract::The skeleton is characterized by anatomic heterogeneity of metabolic turnover. Site-dependent differences in hormonal effects seem likely. Hyporesponsiveness of osteoclasts to parathyroid hormone (PTH) and probably calcitriol was recently demonstrated in the fifth lumbar vertebra of a rat model with moderate renal fai...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(01)00502-6
更新日期:2001-09-01 00:00:00
abstract::Interleukin (IL)-17 is crucial to osteoclast differentiation and activation. Osteocytes support osteoclast formation and are thought to orchestrate bone remodeling in response to fluid flow. The contribution of IL-17 to osteocyte-related bone resorption remains unclear. Here, we used the osteocyte-like MLO-Y4 cell lin...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2017.04.003
更新日期:2017-08-01 00:00:00
abstract::Although low bone mineral density (BMD) is strongly associated with increased fracture risk, up to 50% of those who suffer fractures are not detected as high-risk patients by BMD testing. Thus, new approaches may improve identification of those at increased risk for fracture by in vivo assessment of altered bone tissu...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2015.03.021
更新日期:2018-01-01 00:00:00
abstract::Bone can be viewed as a nano-fibrous composite with complex hierarchical structures. Its deformation and fracture behaviors depend on both the local structure and the type of stress applied. In contrast to the extensive studies on bone fracture under compression and tension, there is a lack of knowledge on the fractur...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2014.10.001
更新日期:2015-02-01 00:00:00
abstract::Despite the potential biological importance of sympathetic activity in human bone metabolism, its effects on bone microarchitecture, a key determinant of bone quality, has not been thoroughly studied. In the present study, we investigated the lumbar spine trabecular bone score (TBS) as an indicator of skeletal deterio...
journal_title:Bone
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1016/j.bone.2018.06.023
更新日期:2018-11-01 00:00:00
abstract::On the basis of studies with bone-seeking isotopes, it is generally believed that the metabolic activity of osteoblasts of the trabecular bone is greater than that of the osteoblasts of the cortex. This implies that the oxidative activity, which provides the energy for biosyntheses, will also be greater in the former ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/8756-3282(89)90061-6
更新日期:1989-01-01 00:00:00
abstract::Due to the increasing number of osteoporotic fractures of hip, spine, and wrist there is a growing need for methods to track down the subjects with inferior bone structure and to monitor the effects of therapeutic measures. This study aims at a noninvasive diagnostic tool, deriving architectural properties of trabecul...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(97)00233-0
更新日期:1998-02-01 00:00:00
abstract::The hips are frequently involved in inheritable diseases which affect the bones. The clinical and radiological presentation of these diseases may be very similar to common hip disorders as developmental dysplasia of the hip, osteoarthritis and avascular necrosis, so the diagnosis may be easily overlooked and treatment...
journal_title:Bone
pub_type: 杂志文章,评审
doi:10.1016/j.bone.2020.115729
更新日期:2021-02-01 00:00:00
abstract:BACKGROUND:Dietary acid charge enhances bone loss. Bicarbonate or alkali diet decreases bone resorption in humans. We compared the effect of an alkaline mineral water, rich in bicarbonate, with that of an acid one, rich in calcium only, on bone markers, in young women with a normal calcium intake. METHODS:This study c...
journal_title:Bone
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.bone.2008.09.007
更新日期:2009-01-01 00:00:00
abstract::Clinical bone sonometers applied at the calcaneus measure broadband ultrasound attenuation and speed of sound. However, the relation of ultrasound measurements to bone strength is not well-characterized. Addressing this issue, we assessed the extent to which ultrasonic measurements convey in vitro mechanical propertie...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2017.06.021
更新日期:2017-10-01 00:00:00
abstract::Avian tibial dyschondroplasia is a disorder of endochondral ossification which results in the accumulation of noncalcified, avascular cartilage. We have investigated the changes in cell proliferation and enzyme activities within specific cell types in the growth plate of chickens with differing severity of the disease...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/8756-3282(92)90362-z
更新日期:1992-01-01 00:00:00
abstract::Metastatic cancer is lethal and patients who suffer bone metastases fare especially poorly. Bone-specific metastatic progression in prostate and breast cancers is a highly observed example of organ-specific metastasis, or organotropism. Though research has delineated the sequential steps of the metastatic cascade, the...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2020.115788
更新日期:2020-12-03 00:00:00
abstract::Bone morphogenetic protein 2 (BMP2), a very potent bone-inducing agent, promotes the differentiation of bone marrow stem cells (BMSCs) to osteoblasts. However, the potency of BMP2 action is variable and its perturbed dynamic signaling pathways in human BMSCs has not been fully elucidated. In this study, we used a comb...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2010.02.013
更新日期:2010-06-01 00:00:00
abstract::Heterotopic ossification (HO) involves formation of endochondral bone at non-skeletal sites, is prevalent in severely wounded service members, and causes significant complications and delayed rehabilitation. As common prophylactic treatments such as anti-inflammatory drugs and irradiation cannot be used after multi-sy...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2016.06.014
更新日期:2016-09-01 00:00:00
abstract:BACKGROUND:Currently, bone densitometry fails to identify nearly half of those elderly patients at immediate fracture risk. To improve clinical assessment of vertebral fracture risk, we aimed to determine how the DXA-based 2D parameter Trabecular Bone Score (TBS) relates to subregional variability in 3D trabecular micr...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2019.02.014
更新日期:2019-05-01 00:00:00
abstract::We believe that the assembled data are consistent with the presence of mRNA species and/or proteins in pagetic bone that are recognized by some paramyxovirus antibodies and nucleic acid probes. The evidence presented so far is insufficient to substantiate claims for the "unequivocal" presence of paramyxovirus sequence...
journal_title:Bone
pub_type: 杂志文章,评审
doi:10.1016/s8756-3282(99)00032-0
更新日期:1999-05-01 00:00:00
abstract::Weekly administration of teriparatide has been shown to reduce the risk of vertebral and non-vertebral fractures in patients with osteoporosis at higher fracture risk in Japan. However, its efficacy for hip fracture has not been established. To gain insight into the effect of weekly teriparatide on the hip, hip struct...
journal_title:Bone
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.bone.2014.04.004
更新日期:2014-07-01 00:00:00
abstract::Measurement of bone mineral density (BMD) is the clinical gold standard in cases of compromised skeletal integrity, such as with osteoporosis. While BMD is a useful measurement to index skeletal health, it is also limited since it cannot directly assess any mechanical properties. The ability to directly assess mechani...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2015.01.019
更新日期:2015-06-01 00:00:00
abstract::Pre-clinical in vivo models of multiple myeloma are essential tools for investigating the pathophysiology of multiple myeloma and for testing new therapeutic agents and strategies prior to their potential use in clinical trials. Over the last five decades, several different types of murine models of multiple myeloma h...
journal_title:Bone
pub_type: 杂志文章,评审
doi:10.1016/j.bone.2015.04.004
更新日期:2015-08-01 00:00:00
abstract:BACKGROUND:Vertebral fractures (VF) upon Denosumab (DMAB) discontinuation were first described as a distinct phenomenon in 2015, yet the magnitude of this event remains undetermined. OBJECTIVES:To estimate fracture risk after DMAB discontinuation, in a real-world setting. METHODS:The computerized database of a 2.3-mi...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2019.115150
更新日期:2020-01-01 00:00:00
abstract::Rough-surfaced substrates made by a variety of methods have been shown to influence osteoblast proliferation and differentiation. The purpose of this study is to confirm the role of surface roughness in promoting osteoblastic differentiation using tissue culture polystyrene as substrate, by excluding factors other tha...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(99)00192-1
更新日期:1999-10-01 00:00:00
abstract::Prostaglandin E(2) (PGE(2)) is essential for fracture healing. Systemic administration of EP4 ligands such as PGE(2) and other synthetic EP4 agonists appears to transduce anabolic signals by binding to receptor EP4. Therefore, the present study was designed to test whether administration of EP4 agonist accelerates the...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2004.01.002
更新日期:2004-06-01 00:00:00
abstract::The parathyroid hormone (PTH) signaling pathways that effect changes in osteoblast gene expression also alter the organization of the cytoskeletal proteins. PTH regulates the expression of nucleoskeletal proteins, such as nuclear mitotic apparatus protein (NuMA) and topoisomerase II-alpha. NuMA is a structural compone...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(97)00300-1
更新日期:1998-04-01 00:00:00