Abstract:
:Bone morphogenetic protein 2 (BMP2), a very potent bone-inducing agent, promotes the differentiation of bone marrow stem cells (BMSCs) to osteoblasts. However, the potency of BMP2 action is variable and its perturbed dynamic signaling pathways in human BMSCs has not been fully elucidated. In this study, we used a combination of stable isotope labeling by amino acids during cell culture (SILAC) and liquid-chromatography electrospray ionization mass spectrometry (LC-ESI-MS/MS) technology to reveal the BMP2 action in BMSC. In this quantitative proteomic analysis, 414 of 449 proteins were successfully quantified with 79.2% peptide quantification efficiency. Interestingly, beta-Catenin was identified in BMP2-stimulated heavy isotope-labeled cells, and further analysis confirmed that BMP2 increased beta-Catenin mRNA and protein levels. The increment effects of BMP2 on the beta-Catenin expression levels and its translocation to nucleus were diminished by blocking the PI3K signal pathway. In addition, BMP2-induced beta-Catenin activity and ALP activity were blocked by PI3K inhibition. Thus, our quantitative proteomics analysis and further biochemical investigations showed that BMP2 modulates beta-Catenin signaling via PI3K pathway and that this pathway plays roles in BMP2-induced osteoblast differentiation of hBMSCs.
journal_name
Bonejournal_title
Boneauthors
Lee JH,Kim BG,Ahn JM,Park HJ,Park SK,Yoo JS,Yates JR 3rd,Cho JYdoi
10.1016/j.bone.2010.02.013subject
Has Abstractpub_date
2010-06-01 00:00:00pages
1522-32issue
6eissn
8756-3282issn
1873-2763pii
S8756-3282(10)00435-7journal_volume
46pub_type
杂志文章相关文献
BONE文献大全abstract::We examined the effect of nicotine on cellular proliferation, as measured by [3H]thymidine (TdR) incorporation and cell count, and on alkaline phosphatase activity in UMR 106-01 rat osteoblastic osteosarcoma cells. The cells were cultured with varying concentrations of nicotine in serum-free medium for 2 to 72 hours. ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/8756-3282(91)90077-v
更新日期:1991-01-01 00:00:00
abstract::Bone has the unique capacity to heal without the formation of a fibrous scar, likely because several of the cellular and molecular processes governing bone healing recapitulate the events during skeletal development. A critical component in bone healing is the timely appearance of blood vessels in the fracture callus....
journal_title:Bone
pub_type: 杂志文章,评审
doi:10.1016/j.bone.2014.09.017
更新日期:2015-01-01 00:00:00
abstract::Bone is a hierarchical, multiphasic and anisotropic structure which in addition possess piezoelectric properties. The generation of piezoelectricity in bone is a complex process which has been shown to play a key role both in bone adaptation and regeneration. In order to understand the complex biological, mechanical a...
journal_title:Bone
pub_type: 杂志文章,评审
doi:10.1016/j.bone.2019.07.024
更新日期:2019-10-01 00:00:00
abstract::Although low bone mineral density (BMD) is strongly associated with increased fracture risk, up to 50% of those who suffer fractures are not detected as high-risk patients by BMD testing. Thus, new approaches may improve identification of those at increased risk for fracture by in vivo assessment of altered bone tissu...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2015.03.021
更新日期:2018-01-01 00:00:00
abstract::We previously reported that a type II sodium phosphate (Na(+)-Pi) cotransporter (Npt2) protein is expressed in osteoclasts and that Pi limitation decreases osteoclast-mediated bone resorption in vitro. We also demonstrated that mice homozygous for the disrupted Npt2 gene (Npt2-/-) exhibit a unique age-dependent bone p...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(01)00601-9
更新日期:2001-11-01 00:00:00
abstract::A characteristic relationship for bone between bone volume fraction (BV/TV) and specific surface (BS/TV) has previously been proposed based on 2D histological measurements. This relationship has been suggested to be bone intrinsic, i.e., to not depend on bone type, bone site and health state. In these studies, only li...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2014.11.016
更新日期:2015-03-01 00:00:00
abstract::Recent in vitro and animal studies suggest that cholesterol and its metabolites inhibit the functional activity of osteoblasts and thereby induce reduced bone mineralization. However, scant information is available on the clinical implication of these findings with special regard to postmenopausal bone loss. Therefore...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(02)00918-3
更新日期:2003-01-01 00:00:00
abstract::Despite the fact that the medications used to treat abnormal bone conditions often induce osteonecrosis of the jaw (ONJ), previous attempts to establish an animal model for ONJ have shown insufficient consideration for this important prerequisite for the development of the disease. The purpose of this study was to est...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2015.08.012
更新日期:2015-12-01 00:00:00
abstract::Trauma-induced heterotopic ossification is an intriguing phenomenon involving the inappropriate ossification of soft tissues within the body such as the muscle and ligaments. This inappropriate formation of bone is highly prevalent in those affected by blast injuries. Here, we developed a simplified cell culture model...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2018.09.008
更新日期:2018-12-01 00:00:00
abstract:PURPOSE:To investigate the effectiveness of the T-score values provided by Radiofrequency Echographic Multi Spectrometry (REMS) in the identification of patients at risk for incident osteoporotic fractures. METHODS:A population of Caucasian women (30-90 years), enrolled from 2013 to 2016, underwent dual X-ray absorpti...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2020.115297
更新日期:2020-05-01 00:00:00
abstract::Growing evidence suggests that positive associations between fat mass (FM) and bone mineral density (BMD) are mediated by not only biomechanical but also biochemical factors. Adiponectin is a novel adipocyte-derived hormone that regulates energy homeostasis and has anti-inflammatory and anti-atherogenic effects. Unlik...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(03)00237-0
更新日期:2003-10-01 00:00:00
abstract:INTRODUCTION:Changes in hip structure and geometry during aging contribute to decreased bone strength. Little is known, however, about these characteristics at advanced age, when fragility fractures are common. We examined hip structural geometry in men and women of old age (72-84 years) and old-old age (85-96 years) t...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2007.06.001
更新日期:2007-10-01 00:00:00
abstract::Histomorphometric changes in the trabecular structure of the principal compressive stress region in the femur in patients with osteoarthritis and fracture of the femoral neck are described. Femoral heads were obtained from patients who had surgery for the treatment of femoral neck fracture or total hip replacement for...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/8756-3282(85)90044-4
更新日期:1985-01-01 00:00:00
abstract::Over 60% of our severely combat-injured patient population develops radiographically apparent heterotopic ossification. Nearly a third of these require surgical excision of symptomatic lesions, a procedure that is fraught with complications, and delays or regresses functional rehabilitation in many cases. Unfortunatel...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2013.08.026
更新日期:2013-12-01 00:00:00
abstract::The musculoskeletal system is significantly more complex than portrayed by traditional reductionist approaches that have focused on and studied the components of this system separately. While bone and skeletal muscle are the two largest tissues within this system, this system also includes tendons, ligaments, cartilag...
journal_title:Bone
pub_type: 杂志文章,评审
doi:10.1016/j.bone.2015.02.010
更新日期:2015-11-01 00:00:00
abstract::Although much is known about hip fracture epidemiology, there are relatively little data regarding fractures at other peripheral sites. Epidemiological differences between fractures are important, since they imply that an understanding of the consequences and clinical expression of osteoporosis requires the study of m...
journal_title:Bone
pub_type: 杂志文章,评审
doi:10.1016/8756-3282(95)00504-8
更新日期:1996-03-01 00:00:00
abstract::Biochemical bone turnover markers (BTMs) provide important information on the diagnosis, therapy and monitoring of metabolic bone diseases including osteoporosis. One goal of antiresorptive therapy in women is to decrease biochemical BTMs to the lower half of reference intervals for healthy pre-menopausal counterparts...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2010.07.017
更新日期:2010-10-01 00:00:00
abstract:BACKGROUND:Currently, bone densitometry fails to identify nearly half of those elderly patients at immediate fracture risk. To improve clinical assessment of vertebral fracture risk, we aimed to determine how the DXA-based 2D parameter Trabecular Bone Score (TBS) relates to subregional variability in 3D trabecular micr...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2019.02.014
更新日期:2019-05-01 00:00:00
abstract::Given the adaptation of bone to prevalent loading, bone loss should follow, but lag behind, the decline in physical performance during aging. Furthermore, bone responsiveness to load-induced strains is believed to decrease with aging. However, the relationship between bone and lean body ( approximately muscle) mass ap...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2009.07.014
更新日期:2009-11-01 00:00:00
abstract:BACKGROUND:Genesis of a cartilaginous scaffold is an obligate precursor to bone formation in heterotopic endochondral ossification (HEO). We tested the hypothesis that cartilage-derived retinoic acid-sensitive protein (CD-RAP) can serve as a plasma biomarker for the pre-osseous cartilaginous stage of HEO. Palovarotene,...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2017.09.016
更新日期:2018-04-01 00:00:00
abstract:INTRODUCTION:Osteocalcin (OC) is the most abundant non-collagenous bone protein and is determinant for bone mineralization. We aimed to compare OC bone expression and serum factors related to its carboxylation in hip fragility fracture and osteoarthritis patients. We also aimed to identify which of these factors were a...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2012.08.129
更新日期:2012-12-01 00:00:00
abstract::Bone strength in adulthood is known to be affected by health at birth and early childhood. Habitual bone loading is a primary determinant of bone strength in later childhood and adulthood. However, the effects of physical activity in early childhood (e.g. crawling, standing and walking) on bone strength are unknown. F...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2014.08.003
更新日期:2014-11-01 00:00:00
abstract:PURPOSE:In the Fracture Prevention Trial, the risks of any nonvertebral fracture (relative risk [RR] 0.65, P=0.04) and any fragility nonvertebral fracture (RR 0.47, P=0.02) were significantly reduced in the teriparatide 20 μg/day (teriparatide) versus placebo group. The purpose of this analysis was to examine the effic...
journal_title:Bone
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.bone.2011.10.018
更新日期:2012-01-01 00:00:00
abstract:INTRODUCTION AND HYPOTHESIS:Diagnosis of prevalent osteoporotic vertebral fracture is complicated by normal or developmental variation in vertebral shape or size and non-osteoporotic deformities that appear to have 'reduced' height. Using our visual approach, the algorithm-based qualitative method (ABQ) a vertebra with...
journal_title:Bone
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1016/j.bone.2007.03.015
更新日期:2007-07-01 00:00:00
abstract::Increases in local and systemic bone resorption are hallmarks of rheumatoid arthritis (RA). Osteoclasts are implicated in these processes and their enhanced differentiation may contribute to bone destruction. We observed that in vitro osteoclastogenesis varies among healthy individuals and hypothesized that increased ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2010.10.167
更新日期:2011-03-01 00:00:00
abstract::Recent studies have established that generalized loss of trabecular bone occurs in the growing rat following day-to-day inflammatory irritation for a period of 3 wk. We can now demonstrate that there are similar effects on bone in milder but more prolonged chronic inflammation (14 wk). Thus, there were significant dec...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/8756-3282(85)90225-x
更新日期:1985-01-01 00:00:00
abstract::Skeletal unloading in an animal hindlimb suspension model and microgravity experienced by astronauts or as a result of prolonged bed rest causes site-specific losses in bone mineral density of 1%-2% per month. This is accompanied by reductions in circulating levels of 1,25-(OH)(2)D(3), the active metabolite of vitamin...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(02)00836-0
更新日期:2002-09-01 00:00:00
abstract::Inflammation is an important part of the fracture repair process which requires osteogenic cells to interact with innate immune cells such as macrophages. All murine macrophages express the F4/80 cell surface marker but they may be further subdivided into two main phenotypes: M1 (proinflammatory) or M2 (anti-inflammat...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2020.115479
更新日期:2020-09-01 00:00:00
abstract::The relationships of bone size, bone strength, and bone formation were investigated in two strains of mice, NZB/B1NJ and RF/J. Measurement of the femur midshaft size by peripheral quantitative computed tomography (pQCT) showed that the RF/J mice had a 32% greater cross-sectional area than NZB/B1NJ mice at 10 weeks of ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(02)00908-0
更新日期:2002-12-01 00:00:00
abstract::Bone mineral density (BMD) is influenced by both environmental and genetic factors. We previously reported the association of the RUNX2 A allele with increased bone mineral density (BMD) and protection against a common form of osteoporotic fracture within a Geelong population. We genotyped 991 women from a Scottish co...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2004.02.004
更新日期:2004-06-01 00:00:00