Identification of secretory immunoglobulin A antibody targets from human milk in cultured cells infected with enteropathogenic Escherichia coli (EPEC).

Abstract:

:Enteropathogenic Escherichia coli (EPEC) uses a type III secretion system (T3SS) to inject effectors into host cells and alter cellular physiology. The aim of the present study was to identify targets of human secretory immunoglobulin A (sIgA) antibodies from the proteins delivered by EPEC into HEp-2 cells after infection. Bacterial proteins delivered into EPEC-infected cells were obtained in sub-cellular fractions (cytoplasmic, membrane, and cytoskeleton) and probed with sIgA antibodies from human milk and analyzed by Western blotting. These sIgA antibodies reacted with Tir and EspB in the cytoplasmic and membrane fractions, and with intimin in the membrane fraction mainly. The sIgA also identified an EPEC surface-associated Heat-shock protein 70 (Hsp70) in HEp-2 cells infected with EPEC. Purified Hsp70 from EPEC was able to bind to HEp-2 cells, suggesting adhesive properties in this protein. EspC secreted to the medium reacted strongly with the sIgA antibodies. An EPEC 115 kDa protein, unrelated to the EspC protein, was detected in the cytoplasm of infected HEp-2 cells, suggesting that this is a new protein translocated by EPEC. The results suggest that there is a strong host antibody response to Tir and intimin, which are essential proteins for attaching and effacing (A/E) pathogen mediated disease.

journal_name

Microb Pathog

journal_title

Microbial pathogenesis

authors

Gavilanes-Parra S,Mendoza-Hernández G,Chávez-Berrocal ME,Girón JA,Orozco-Hoyuela G,Manjarrez-Hernández A

doi

10.1016/j.micpath.2013.09.001

subject

Has Abstract

pub_date

2013-11-01 00:00:00

pages

48-56

eissn

0882-4010

issn

1096-1208

pii

S0882-4010(13)00120-4

journal_volume

64

pub_type

杂志文章
  • Rhodomyrtone decreases Staphylococcus aureus SigB activity during exponentially growing phase and inhibits haemolytic activity within membrane vesicles.

    abstract::Sigma factor B (SigB) controls the expression of Staphylococcus aureus genes including virulence factors and plays a role in the bacterial secretion system through membrane vesicle production. Inhibition of SigB could attenuate SigB dependent virulence and secretion system. The objective of this study was to determine...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2018.12.019

    authors: Mitsuwan W,Jiménez-Munguía I,Visutthi M,Sianglum W,REIPI\/GEIH Study Group.,Rodríguez-Ortega MJ,Voravuthikunchai SP

    更新日期:2019-03-01 00:00:00

  • Lithium chloride confers protection against viral myocarditis via suppression of coxsackievirus B3 virus replication.

    abstract::Viral myocarditis (VMC) is a type of inflammation affecting myocardial cells caused by viral infection and has been an important cause of dilated cardiomyopathy (DCM) worldwide. Type B3 coxsackievirus (CVB3), a non-enveloped positive-strand RNA virus of the Enterovirus genus, is one of most common agent of viral myoca...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2020.104169

    authors: Zhao Y,Yan K,Wang Y,Cai J,Wei L,Li S,Xu W,Li M

    更新日期:2020-07-01 00:00:00

  • hsp65 mRNA+ macrophages and gamma delta T cells in influenza virus-infected mice depleted of the CD4+ and CD8+ lymphocyte subsets.

    abstract::The effects of depleting CD4+ and CD8+ T cells on macrophage recruitment have been analyzed for bronchoalveolar lavage (BAL) populations from mice with primary or secondary influenza pneumonia. Macrophages were characterized by both the capacity to engulf latex particles and the expression of mRNA for a 65 kD heat sho...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1006/mpat.1993.1008

    authors: Allan W,Carding SR,Eichelberger M,Doherty PC

    更新日期:1993-01-01 00:00:00

  • Molecular and biochemical mechanisms of Pasteurella haemolytica leukotoxin-induced cell death.

    abstract::Pasteurella haemolytica leukotoxin (LKT) is a member of the RTX family of pore-forming toxins that kill bovine immune cells. Several studies have suggested that RTX toxins kill target cells by the induction of apoptosis. In the present study, BL3 bovine leukaemia cells were exposed to LKT and assessed by molecular and...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1006/mpat.1998.0236

    authors: Wang JF,Kieba IR,Korostoff J,Guo TL,Yamaguchi N,Rozmiarek H,Billings PC,Shenker BJ,Lally ET

    更新日期:1998-12-01 00:00:00

  • Isolates of the Enterobacter cloacae complex induce apoptosis of human intestinal epithelial cells.

    abstract::Strains of the Enterobacter cloacae complex are becoming increasingly important human pathogen. The aim of the study was to identify, by sequencing the hsp60 gene, the species of clinical isolates phenotypically identified as E. cloacae and to examine them for virulence-associated properties: the ability of adhesion, ...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2010.04.003

    authors: Krzymińska S,Koczura R,Mokracka J,Puton T,Kaznowski A

    更新日期:2010-09-01 00:00:00

  • Intercellular adhesion and biocide resistance in nontypeable Haemophilus influenzae biofilms.

    abstract::Respiratory infections caused by nontypeable Haemophilus influenzae (NTHi) are a major medical problem. Evidence suggests that the ability to form biofilms on mucosal surfaces may play a role in NTHi pathogenesis. However, the factors that contribute to NTHi biofilm cohesion remain largely unknown. In this study we in...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2009.01.004

    authors: Izano EA,Shah SM,Kaplan JB

    更新日期:2009-04-01 00:00:00

  • Genome-wide identification of genes regulated by RcsA, RcsB, and RcsAB phosphorelay regulators in Klebsiella pneumoniae NTUH-K2044.

    abstract::Rcs phosphorelay system is a two-component signal transduction system, which can regulate the transcription of capsule polysaccharide and biofilm related genes in Enterobacteriaceae. In this study, microarray technology was used to investigate the overall genes regulated by RcsA, RcsB, and RcsAB and the regulation mec...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2018.06.036

    authors: Su K,Zhou X,Luo M,Xu X,Liu P,Li X,Xue J,Chen S,Xu W,Li Y,Qiu J

    更新日期:2018-10-01 00:00:00

  • Reduced virulence of a hfq mutant of Pseudomonas aeruginosa O1.

    abstract::The Sm-like protein Hfq has been implicated in the regulation of sigmaS-dependent and sigmaS-independent genes in E. coli and in the regulation of virulence factors in both, Yersinia enterocolitica and Brucella abortus. Here, we have studied the effect of Hfq on virulence and stress response of Pseudomonas aeruginosa ...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/s0882-4010(03)00149-9

    authors: Sonnleitner E,Hagens S,Rosenau F,Wilhelm S,Habel A,Jäger KE,Bläsi U

    更新日期:2003-11-01 00:00:00

  • Increased monokines in cytomegalovirus infected myelomonocytic cell cultures.

    abstract::In an investigation of the role of monokines in CMV associated immunosuppression we document modulation of both TNF and IL-1 beta in CMV infected THP-1 cells. CMV infected cultures released almost two-fold more IL-1 beta protein and contained significantly higher IL-1 beta mRNA levels than uninfected cultures for 72-9...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/0882-4010(89)90032-6

    authors: Turtinen LW,Assimacopoulos A,Haase AT

    更新日期:1989-08-01 00:00:00

  • Transcription of bovine leukemia virus in peripheral blood cells obtained during early infection in vivo.

    abstract::Bovine leukemia virus (BLV) is transcriptionally silent in most circulating peripheral blood mononuclear cells (PBMCs) of animals with well-established infections. Using PBMCs from a newly infected sheep, we asked whether viral transcription proceeded differently during the initial months of infection, when the preval...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/0882-4010(92)90095-6

    authors: Radke K,Sigala TJ,Grossman D

    更新日期:1992-05-01 00:00:00

  • Essential role of toll-like receptor 4 in Acinetobacter baumannii-induced immune responses in immune cells.

    abstract::TLR4 is a membrane sensor for lipopolysaccharide (LPS), a major cell wall component of gram-negative bacteria. In this study, we investigated the role of TLR4 on innate immune responses in immune cells against Acinetobacter baumannii. Bone marrow-derived macrophages (BMDMs) and dendritic cells (BMDCs) were isolated fr...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2012.08.008

    authors: Kim CH,Jeong YJ,Lee J,Jeon SJ,Park SR,Kang MJ,Park JH,Park JH

    更新日期:2013-01-01 00:00:00

  • Green engineered biomolecule-capped silver and copper nanohybrids using Prosopis cineraria leaf extract: Enhanced antibacterial activity against microbial pathogens of public health relevance and cytotoxicity on human breast cancer cells (MCF-7).

    abstract::This research focused on green engineering and characterization of silver (PcAgNPs) and copper nanoparticles (PcCuNPs) using Prosopis cineraria (Pc) leaf extract prepared by using microwave irradiation. We studied their enhanced antimicrobial activity on human pathogens as well as cytotoxicity on breast cancer cells (...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2017.02.019

    authors: Jinu U,Gomathi M,Saiqa I,Geetha N,Benelli G,Venkatachalam P

    更新日期:2017-04-01 00:00:00

  • Evidence for clathrin mobilization during directed phagocytosis of Shigella flexneri by HEp2 cells.

    abstract::The enteroinvasive species Shigella flexneri expresses a plasmid-mediated capacity to penetrate epithelial cells by directed phagocytosis involving actin polymerization. In the present work, HEp2 cells were depleted of intracellular K+ in order to arrest receptor-mediated endocytosis and to evaluate the role of this e...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/0882-4010(89)90036-3

    authors: Clerc PL,Sansonetti PJ

    更新日期:1989-11-01 00:00:00

  • Computer aided ligand based screening for identification of promising molecules against enzymes involved in peptidoglycan biosynthetic pathway from Acinetobacter baumannii.

    abstract::A. baumannii has been considered as Priority-I as suggested by the World Health Organization (WHO) and the most critical pathogenic microorganism for causing nosocomial infection in imunno-compromised hospital-acquired patients due to multi-drug resistance (MDR). In the current study, we utilized "Computer-aided ligan...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2020.104205

    authors: Amera GM,Khan RJ,Pathak A,Jha RK,Muthukumaran J,Singh AK

    更新日期:2020-10-01 00:00:00

  • The role of O1-antigen in the adhesion to uroepithelial cells of Klebsiella pneumoniae grown in urine.

    abstract::We obtained mutants devoid of the O1-antigen, the capsular polysaccharide (K antigen) or both from Klebsiella pneumoniae clinical isolates (urinary infection). These mutants were grown in urine, and their ability to fimbriate and to adhere were studied. Mutants lacking the O1-antigen, independently of the other surfac...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1006/mpat.1997.0132

    authors: Merino S,Rubires X,Aguilar A,Tomás JM

    更新日期:1997-07-01 00:00:00

  • Monoclonal antibodies recognizing the Enterococcus faecalis collagen-binding MSCRAMM Ace: conditional expression and binding analysis.

    abstract::Enterococci are opportunistic pathogens known to cause numerous clinical infections and complications in humans. Adhesin-mediated binding to extracellular matrix (ECM) proteins of the host is thought to be a crucial step in the pathogenesis of these bacterial infections. Adhesin of collagen from Enterococcus faecalis ...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2007.03.003

    authors: Hall AE,Gorovits EL,Syribeys PJ,Domanski PJ,Ames BR,Chang CY,Vernachio JH,Patti JM,Hutchins JT

    更新日期:2007-08-01 00:00:00

  • alpha-Hydroxynitrile lyase protein from Xylella fastidiosa: Cloning, expression, and characterization.

    abstract::Xylella fastidiosa is a xylem-restricted plant pathogen that causes a range of diseases in several and important crops. Through comparative genomic sequence analysis many genes were identified and, among them, several potentially involved in plant-pathogen interaction. The experimental determination of the primary seq...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2009.06.007

    authors: Caruso CS,de Fátima Travensolo R,de Campus Bicudo R,de Macedo Lemos EG,Ulian de Araújo AP,Carrilho E

    更新日期:2009-09-01 00:00:00

  • WITHDRAWN: Paeoniflorin attenuates LPS-induced inflammation in nucleus pulposus cells via Nrf-2/HO-1/HMGB1/NF-κB pathway.

    abstract::This article has been withdrawn at the request of the author(s) and/or editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at https://www.elsevier.com/about/our-business/policies/article-withdrawal. ...

    journal_title:Microbial pathogenesis

    pub_type: 撤回出版物

    doi:10.1016/j.micpath.2018.08.009

    authors: Heng Y,Mao W,Yong M,Yang G,Su Q,Wang J

    更新日期:2018-08-08 00:00:00

  • Expression of non-replicating persistence associated genes of Mycobacterium bovis in lymph nodes from skin test-reactor cattle.

    abstract::Upon oxygen shift-down, Mycobacterium tuberculosis complex bacteria can induce a genetic program characterized by halted duplication, which is called Non-replicating persistence (NRP). During this phase, at least 48 genes, collectively named Dormancy survival regulator (DosR) regulon, are important for the long-term s...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2013.04.012

    authors: Jiménez BA,Hinojoza-Loza E,Flores-Valdez MA,Prado-Montes de Oca E,Allen K,Estrada-Chávez C,Herrera-Rodríguez S,Flores-Fernández JM,Martínez-Velázquez M,Hernández-Gutiérrez R,Alvarez AH

    更新日期:2013-08-01 00:00:00

  • Antimicrobial activity of crude fractions and morel compounds from wild edible mushrooms of North western Himalaya.

    abstract::The antimicrobial properties of morel compounds from wild edible mushrooms (Morchella esculenta and Verpa bohemica) from Kashmir valley was investigated against different clinical pathogens. The butanol crude fraction of most popular or true morel M. esculenta showed highest 19 mm IZD against E.coli while as same frac...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2017.03.005

    authors: Shameem N,Kamili AN,Ahmad M,Masoodi FA,Parray JA

    更新日期:2017-04-01 00:00:00

  • Expression of the Escherichia coli IrgA homolog adhesin is regulated by the ferric uptake regulation protein.

    abstract::The IrgA homolog adhesin (Iha) is an adherence-conferring outer membrane protein of Escherichia coli associated with enterohemorrhagic and uropathogenic strains. Here, we used primer extension analysis to identify iha promoters in O157:H7 and uropathogenic E. coli strains. Transcriptional fusions demonstrated that iha...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2006.07.006

    authors: Rashid RA,Tarr PI,Moseley SL

    更新日期:2006-12-01 00:00:00

  • Listeriolysin S may inhibit the anti-listerial properties of Lactobacillus plantarum.

    abstract::Listeriosis is a serious infection linked to the consumption of food contaminated with Listeria monocytogenes. Outbreaks and mortality rates associated with this infection make it a significant public health concern. As biocontrol agents, probiotics such as Lactobacillus plantarum had been of interest for the promotio...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2019.103744

    authors: Mohammadzadeh R,Azadegan A,Kalani BS

    更新日期:2019-12-01 00:00:00

  • Isolation and characterization of a broad-spectrum phage of multiple drug resistant Salmonella and its therapeutic utility in mice.

    abstract::Salmonella are causes of livestock, poultry, and other animal diseases but they also have the potential to infect people. Currently, antibiotics are the first choice for treatment of Salmonella infections. Thus, the utility of phage has become the research focus for scientists for several reasons. There are efficient,...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2018.10.042

    authors: Tang F,Zhang P,Zhang Q,Xue F,Ren J,Sun J,Qu Z,Zhuge X,Li D,Wang J,Jiang M,Dai J

    更新日期:2019-01-01 00:00:00

  • Genotype distribution of Chinese Mycoplasma bovis isolates and their evolutionary relationship to strains from other countries.

    abstract::This study was undertaken to determine the genotypic distribution of Chinese M. bovis strains and their similarity to isolates from other countries. Two multilocus sequence typing (MLST) schemes (MLST-1 and MLST-2) and pulsed field gel electrophoresis (PFGE) were used to compare 44 Chinese strains and the M. bovis typ...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2017.08.029

    authors: Menghwar H,He C,Zhang H,Zhao G,Zhu X,Khan FA,Faisal M,Rasheed MA,Zubair M,Memon AM,Ridley A,Robertson ID,Chen Y,Guo A

    更新日期:2017-10-01 00:00:00

  • Effect of CD40 ligand and other immunomodulators on Pneumocystis carinii infection in rat model.

    abstract::The corticosteroid-treated animal is well established as an experimental model for the study of Pneumocystis carinii pneumonitis (PCP). Latent or acquired infection with P. carinii in the murine lung progresses to fatal pneumonitis when the host is profoundly immunocompromized. In this study the effects of five immuno...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1006/mpat.2000.0374

    authors: Oz HS,Hughes WT,Rehg JE,Thomas EK

    更新日期:2000-09-01 00:00:00

  • The 19-kDa antigen of Mycobacterium tuberculosis is a major adhesin that binds the mannose receptor of THP-1 monocytic cells and promotes phagocytosis of mycobacteria.

    abstract::Identification of mycobacterial adhesins is needed to understand better the pathogenesis of tuberculosis and to develop new strategies to fight this infection. In this work, THP-1 monocytic cells were incubated with Mycobacterium tuberculosis culture filtrate proteins labelled with biotin and a dominant 19-kDa adhesin...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2005.06.002

    authors: Diaz-Silvestre H,Espinosa-Cueto P,Sanchez-Gonzalez A,Esparza-Ceron MA,Pereira-Suarez AL,Bernal-Fernandez G,Espitia C,Mancilla R

    更新日期:2005-09-01 00:00:00

  • Experimental infection of golden hamsters with Guama virus (Peribunyaviridae, Orthobunyavirus).

    abstract::The Guama virus (GMAV) is a member of Peribunyaviridae family, Orthobunyavirus genus. Several strains of the virus were isolated in South and Central Americas from several hosts, such as humans, wild animals, including nonhuman primates, wild rodents and mosquitoes as well as mice used as sentinels. The virus is able ...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2019.103627

    authors: Matos GC,Ferreira MS,Martins Filho AJ,Amador Neto OP,Campos VM,Lima MLG,Rodrigues JCP,Ribeiro ACDS,Freitas MNO,Silva FA,Chiang JO,Casseb LMN,Sousa JR,Quaresma JAS,Martins LC,Vasconcelos PFDC,Carvalho VL

    更新日期:2019-10-01 00:00:00

  • Elevated neutrophil, macrophage and dendritic cell numbers characterize immune cell populations in mice chronically infected with Salmonella.

    abstract::The present study characterizes immune cell populations in mice chronically infected with Salmonella. Mice were characterized as chronically infected based on persistently high titers of Salmonella-reactive immunoglobulins in the serum >6 months after a single oral dose of S. enterica serovar Typhimurium. These mice h...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2006.03.004

    authors: Johansson C,Ingman M,Jo Wick M

    更新日期:2006-08-01 00:00:00

  • Analysis of the interaction of group A streptococci with fibrinogen, streptokinase and plasminogen.

    abstract::Group A streptococci demonstrate a number of distinct ways to interact with the human fibrinolytic system to acquire unregulatable cell-surface enzymatic activity. Interactions between bacteria, fibrinogen, streptokinase and plasminogen resulted in acquisition of cell-associated enzymatic activity that can lyse fibrin...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/s0882-4010(95)90013-6

    authors: Wang H,Lottenberg R,Boyle MD

    更新日期:1995-03-01 00:00:00

  • Evaluation of a Yersinia pestis mutant impaired in a thermoregulated type VI-like secretion system in flea, macrophage and murine models.

    abstract::Type VI secretion systems (T6SSs) have been identified recently in several Gram-negative organisms and have been shown to be associated with virulence in some bacterial pathogens. A T6SS of Yersinia pestis CO92 (locus YPO0499-YPO0516) was deleted followed by investigation of the phenotype of this mutation. We observed...

    journal_title:Microbial pathogenesis

    pub_type: 杂志文章

    doi:10.1016/j.micpath.2009.08.005

    authors: Robinson JB,Telepnev MV,Zudina IV,Bouyer D,Montenieri JA,Bearden SW,Gage KL,Agar SL,Foltz SM,Chauhan S,Chopra AK,Motin VL

    更新日期:2009-11-01 00:00:00