Abstract：
:Bovine leukemia virus (BLV) is transcriptionally silent in most circulating peripheral blood mononuclear cells (PBMCs) of animals with well-established infections. Using PBMCs from a newly infected sheep, we asked whether viral transcription proceeded differently during the initial months of infection, when the prevalence of BLV-infected cells and the host's immunological response change markedly. Shortly after being injected with BLV, the animal displayed a characteristic, transient increase in PBMCs that transcribed BLV when cultured. Even when transcriptionally competent PBMCs were most prevalent (1.2%), only rare cells in the circulation (1 in 50,000) contained enough BLV transcripts to be identified readily by in situ hybridization. However, at one point several weeks later, some PBMCs appeared to contain small amounts of BLV RNA as soon as they had been purified from blood. Throughout this period, BLV-transcribing PBMCs greatly outnumbered virus-producing cells, which were counted using a new infectious centers assay. Its viscous medium reduced cell to cell contact among PBMCs, enabling increased detection of BLV-producing cells at a time when virus-specific killer cells might be active. Early infection was polyclonal, and most infected PBMCs transcribed BLV upon being cultured. By 2 months after infection, provirus-containing cells were as abundant as they had been earlier, but few cells transcribed BLV. These results suggest that BLV-infected cells are more easily stimulated to transcribe the provirus and produce infectious virus during the early months of a new infection.
journal_name
Microb Pathogjournal_title
Microbial pathogenesisauthors
Radke K,Sigala TJ,Grossman Ddoi
10.1016/0882-4010(92)90095-6subject
Has Abstractpub_date
1992-05-01 00:00:00pages
319-31issue
5eissn
0882-4010issn
1096-1208pii
0882-4010(92)90095-6journal_volume
12pub_type
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journal_title：Microbial pathogenesis
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1006/mpat.1994.1013
更新日期：1994-02-01 00:00:00
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doi：10.1016/0882-4010(91)90115-q
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1016/0882-4010(89)90032-6
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journal_title：Microbial pathogenesis
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pub_type： 杂志文章
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更新日期：2014-01-01 00:00:00
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journal_title：Microbial pathogenesis
pub_type： 杂志文章
doi：10.1006/mpat.1995.0074
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abstract：:Recent research has shown broad antifungal activity of the classic antidepressants selective serotonin reuptake inhibitors (SSRIs). This fact, combined with the increased cross-resistance frequency of the genre Candida regarding the main treatment today, fluconazole, requires the development of novel therapeutic strat...
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doi：10.1016/j.micpath.2017.04.008
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