Abstract:
:Stable myoblast cell lines were isolated after a brief exposure of mouse fibroblasts (10T1/2 cells) to 5-azacytidine. We show that transfection of 10T1/2 cells with DNA from these azacytidine-induced myoblasts (or from mouse C2C12 myoblasts) results in myogenic conversion of approximately 1 in 15,000 transfected colonies. In contrast, transfection of 10T1/2 cells with DNA from nonmyogenic cells (parental 10T1/2 cell DNA) does not give rise to myoblast colonies. These results indicate that an azacytidine-induced structural modification (presumably demethylation) in the DNA of a single locus is sufficient to convert 10T1/2 cells into determined myoblasts.
journal_name
Celljournal_title
Cellauthors
Lassar AB,Paterson BM,Weintraub Hdoi
10.1016/0092-8674(86)90507-6subject
Has Abstractpub_date
1986-12-05 00:00:00pages
649-56issue
5eissn
0092-8674issn
1097-4172pii
0092-8674(86)90507-6journal_volume
47pub_type
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