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Maternal low-protein diet during lactation combined with early overfeeding impair male offspring's long-term glucose homeostasis.

Abstract：

PURPOSE:The early-life nutritional environment affects long-term glucose homeostasis, we investigated the effects of maternal low-protein diet combined with postnatal early overfeeding on the male offspring's glucose homeostasis in adulthood. METHODS:Only male rats were used, and their delivery was considered postnatal-day 0 (PN0). Wistar rats' dams were divided into control (NP) or low-protein diet (LP). LP dams remained on the diet until PN14, after which all animals were supplied with the control diet. At PN2, litters were adjusted to 9 (control-NL) or 3 (postnatal-overfeeding-PO) pups, resulting in four experimental groups: NP-NL, NP-PO, LP-NL, and LP-PO. Litters were weaned on PN21. At PN80, a batch of animals from all experimental groups underwent surgery for cannula implantation, followed by intravenous glucose tolerance test (ivGTT), but the insulinogenic index (ISI) was calculated. At PN81, animals were euthanized and tissues were collected. RESULTS:LP-diet and early postnatal-overfeeding were effective in promoting the expected biometric outcomes at PN21 and PN81, but the LP-PO animals present a biometric profile similar to the control (NP-NL) group. Postnatal-overfeeding increased fasting glycemia in LP-PO animals (p < 0.01). In the ivGTT, postnatal-overfeeding elevated the glycemia (p < 0.0001), exacerbated in LP-PO animals (p < 0.0001). Insulinemia was reduced by both, maternal LP-diet and postnatal-overfeeding, with a higher degree of reduction in LP-PO animals (p < 0.0001). Maternal LP-diet and postnatal-overfeeding reduced the ISI (p < 0.0001). Factors interaction lead the LP-PO to a lower ISI compared to all other groups (p < 0.0001). CONCLUSIONS:The combination of low-protein diet in breastfeeding dams with postnatal overfeeding disturbed the offspring's glucose metabolism in adulthood.

journal_name

Endocrine

journal_title

Endocrine

authors

Almeida DL,Simões FS,Saavedra LPJ,Praxedes Moraes AM,Matiusso CCI,Malta A,Palma-Rigo K,Mathias PCF

doi

10.1007/s12020-018-1719-9

subject

Has Abstract

pub_date

2019-01-01 00:00:00

pages

62-69

issue

eissn

1355-008X

issn

1559-0100

pii

10.1007/s12020-018-1719-9

journal_volume

pub_type

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Maternal low-protein diet during lactation combined with early overfeeding impair male offspring's long-term glucose homeostasis.