Abstract:
:Gonadotropin-releasing hormone (GnRH) effects on the lactotroph function have been widely studied, but they probably result from paracrine interactions. No visual data about GnRH receptor in the pituitary are available. In order to identify the GnRH target cells in the pituitary of adult rats, the cellular distribution of rat GnRH receptor mRNA was investigated by electron microscopy, usingin situ hybridization on ultrathin pituitary frozen sections.In situ hybridization was performed using a digoxigenin-labeled oligonucleotide probe revealed by an indirect immunogold reaction. Gonadotropin-releasing hormone receptor mRNA was found in the cytoplasmic matrix, apposed to the endoplasmic reticulum and the nucleus of the gonadotrophs, which were identified by their ultrastructural characteristics, and by the presence of luteinizing hormone (LH) immunoreactivity. It was also found in the lactotrophs, which were revealed by the immunocytological detection of prolactin. No GnRH receptor mRNA was detected in corticotrophs, somatotrophs, thyrotrophs or hepatocytes. This result, without excluding paracrine effects, clearly showed that in addition to the gonadotrophs, the lactototrophs are likely to be direct target cells for the hypothalamic GnRH.
journal_name
Endocrinejournal_title
Endocrineauthors
Mertani HC,Testart C,Ouhtit A,Brisson C,Morel Gdoi
10.1007/BF02782760subject
Has Abstractpub_date
1996-04-01 00:00:00pages
159-63issue
2eissn
1355-008Xissn
1559-0100journal_volume
4pub_type
杂志文章相关文献
ENDOCRINE文献大全abstract::Type 2 diabetes mellitus (DM) carries an increased risk for cardiovascular complications. The brachial-ankle pulse wave velocity (baPWV) is an index for early atherosclerotic changes. Recently, the effect of altered blood rheology on atherosclerosis has received attention. Therefore, this study aimed to examine the as...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/s12020-014-0451-3
更新日期:2015-05-01 00:00:00
abstract::Fibroadenomas are the most common benign breast tumors, occurring mainly in young women. Their responses to the hormonal environment are similar to those of normal breast tissue, which suggests that steroid receptors may play a role in tumor development. We evaluated the gene and protein expression of progesterone rec...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/s12020-009-9176-0
更新日期:2009-06-01 00:00:00
abstract:PURPOSE:Metabolic syndrome (MetS) is a constellation of clinical factors that indicates elevated risk of diabetes. It is diagnosed based on three or more abnormalities in its components. This does not take into account that MetS can likely present as a continuum of risk. We aim to develop a MetS severity score and asse...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/s12020-019-01970-5
更新日期:2019-07-01 00:00:00
abstract:PURPOSE:Recently, it has been confirmed that circulating miRNAs play an important role in disease pathogenesis and can be biomarkers of many autoimmune diseases. However, the knowledge about circulating miRNAs in Graves' disease (GD) is very limited. In this study, we aimed to identify circulating miRNAs as potential b...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/s12020-019-01884-2
更新日期:2019-07-01 00:00:00
abstract::Concentrations of immunoreactive (ir-) inhibin in circulation, amniotic fluid, and testes of embryos and newly hatched ducks were determined from d 21 of incubation to d 1 of age. Plasma concentrations of FSH and LH were also determined by chicken radioimmunoassay (RIA) systems. In addition, gene expression and cellul...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1385/ENDO:28:2:171
更新日期:2005-11-01 00:00:00
abstract::Alendronate is a well-established treatment for osteoporosis and suppresses bone resorption by a direct effect on osteoclasts and their precursors. The effect of alendronate on osteoclasts is produced, at least in part, by the receptor activator of nuclear factor kappaB ligand (RANKL) and the osteoprotegerin (OPG) syn...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/s12020-009-9306-8
更新日期:2010-04-01 00:00:00
abstract::Estrogens regulate gene expression and cell proliferation in target tissues. In studies of estrogen-regulated gene expression, identification of appropriate housekeeping genes (HKGs), reference genes whose expression is not altered by treatment, is difficult. The goal of this study was to define HKGs unaltered by estr...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/s12020-009-9154-6
更新日期:2009-04-01 00:00:00
abstract::Vertebral fractures are an emerging complication of acromegaly but their prediction is still difficult occurring even in patients with normal bone mineral density. In this study we evaluated the ability of high-resolution cone-beam computed tomography to provide information on skeletal abnormalities associated with ve...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/s12020-016-1078-3
更新日期:2016-11-01 00:00:00
abstract::Factors affecting longevity are complex and poorly understood. We have recently found that Ames dwarf mice (df/df), which are deficient in growth hormone (GH), prolactin, and thyroid-stimulating hormone, live significantly longer than their normal siblings whereas transgenic mice that overexpress GH exhibit reduced li...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1385/ENDO:11:1:41
更新日期:1999-08-01 00:00:00
abstract:PURPOSE:Parathyroid carcinoma (PCa) is a rare endocrine malignancy with poor prognosis and is often difficult to accurately diagnose both before and after surgery. Dysregulated microRNA (miRNA) levels have been identified in PCa using a limited number of samples. The aim of the present study was to verify a group of mi...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/s12020-018-1551-2
更新日期:2018-05-01 00:00:00
abstract:PURPOSE:Serum intact parathyroid hormone (iPTH) level is an early marker of post-thyroidectomy hypocalcemia. However, lack of methodological standardization to define timing and cut-off points of iPTH measurement limit its clinical applicability. Here, we evaluated the relationship between two distinct postoperative ti...
journal_title:Endocrine
pub_type: 临床试验,杂志文章
doi:10.1007/s12020-018-1601-9
更新日期:2018-08-01 00:00:00
abstract::To investigate the specific mutations in PROP1, POU1F1, LHX3, and HESX1 genes in patients with combined pituitary hormone deficiency (CPHD) in Turkey. Seventy-six patients with CPHD were included in this study. Based on clinical, hormonal, and neuro-radiological data, relevant transcription factor genes were evaluated...
journal_title:Endocrine
pub_type: 杂志文章,多中心研究
doi:10.1007/s12020-014-0498-1
更新日期:2015-06-01 00:00:00
abstract::The adipocyte-derived catabolic protein leptin alters cell-mediated immunity and cytokine crosstalk. This may provide new insights into the altered immune response, seen in obese individuals. Therefore, we determined the tissue distribution of immune cells in diet-induced obese (dio) and normal weight F344 rats challe...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/s12020-010-9358-9
更新日期:2010-08-01 00:00:00
abstract::RF amide peptide family with distinctive terminal -Arg-Phe-NH(2) signature is evolutionarily conserved from invertebrates to mammals. These neuropeptides have been shown to affect diverse functions in invertebrates and vertebrates including influencing pituitary hormone secretion. More recently, two members of this fa...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/s12020-012-9672-5
更新日期:2012-12-01 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Endocrine
pub_type: 已发布勘误
doi:10.1007/s12020-020-02514-y
更新日期:2020-12-01 00:00:00
abstract::We have shown that gonadotrophs synthesize and secrete immunoreactive calcitonin (CT)-like peptide, and CT is a potent inhibitor of prolactin (PRL) secretion and gene transcription. CT cDNA cloned from LssT2 cells (pit-CT cDNA) exhibits 99% homology with mouse CT cDNA sequence, but exhibits four mismatches in the codi...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/s12020-007-0044-5
更新日期:2007-06-01 00:00:00
abstract::Lactogen-dependent Nb2 lymphoma cells, widely employed for studying prolactin (PRL) mitogenic mechanisms, are also useful for investigations of apoptosis in T-lineage lymphocytes. Utilizing PRL-dependent Nb2-11 cultures, apoptosis-regulatory genes were evaluated for participation in dexamethasone- (DEX) provoked cell ...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1385/ENDO:9:2:163
更新日期:1998-10-01 00:00:00
abstract::The thyrotropin receptor is of fundamental importance to normal thyroid function and is considered to be the predominant antigen affected by the autoantibodies of Graves' autoimmune hyperthyroidism. The identification of the epitopes on the receptor to which the autoantibodies bind or the mechanism by which the autoan...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/BF02994449
更新日期:1995-03-01 00:00:00
abstract::Plasma levels for insulin-like growth factor-I (IGF-I) steadily increase in female rats between 20 and 40 d of life, and this increase is intimately related to the wellknown growth spurt occurring at this age. Since specific actions of IGF-I related to sexual function have been described at the ovarian and hypothalami...
journal_title:Endocrine
pub_type: 杂志文章
doi:
更新日期:1997-02-01 00:00:00
abstract::Altered bone micro-architecture is an important factor in accounting for fragility fractures. Until recently, it has not been possible to gain information about skeletal microstructure in a way that is clinically feasible. Bone biopsy is essentially a research tool. High-resolution peripheral Quantitative Computed Tom...
journal_title:Endocrine
pub_type: 杂志文章,评审
doi:10.1007/s12020-014-0280-4
更新日期:2014-11-01 00:00:00
abstract::The present study was designed to determine whether an association exists between HPA activity and cytokine production in major depression (MD). In 9 patients with MD and 11 control subjects of both sexes, all drug-free, activity of the HPA axis was evaluated by circadian rhythm of plasma cortisol, 24-h free urinary c...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/s12020-006-0016-1
更新日期:2006-12-01 00:00:00
abstract::Many new techniques are rapidly being developed and applied to the study of normal and neoplastic pituitary. DNA microarrays are a uniquely efficient method for simultaneously assessing the expression levels of thousands of genes, identifying disease subphenotypes, and predicting disease progression. This article revi...
journal_title:Endocrine
pub_type: 杂志文章,评审
doi:10.1385/ENDO:28:1:049
更新日期:2005-10-01 00:00:00
abstract:PURPOSE:An increasing body of evidence suggests that dipeptidyl-peptidase 4 (DPP-4) inhibitors could play a role in the development of bullous pemphigoid. The knowledge regarding this association is based on case reports, pharmacovigilance database analyses, and observational studies. Data from randomized clinical tria...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/s12020-020-02272-x
更新日期:2020-09-01 00:00:00
abstract:PURPOSE:To describe sex-related differences in self-care; to identify determinants of self-care according to sex, and to investigate how sex interacts with the effect of clinical and socio-demographic variables on self-care in adults with Type 2 Diabetes Mellitus (T2DM). METHODS:Cross-sectional multicentre study with ...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/s12020-020-02189-5
更新日期:2020-02-01 00:00:00
abstract::To evaluate whether continuous subcutaneous insulin infusion (CSII) may have any advantage over multiple daily injections (MDI) on glycemic control and treatment satisfaction in young patients with Type 1 diabetes in transition to an adult diabetes center. The study population consisted of 125 patients on MDI; 38 out ...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/s12020-013-0060-6
更新日期:2014-06-01 00:00:00
abstract::In randomized controlled trials (RCTs), more intensive glucose control in patients with type 2 diabetes leads to a modest (9%) reduction in major cardiovascular events (MACE), associated with a 20% reduction of kidney events and 13% reduction of eye events. The FDA issued guidance in 2008 led to the conduct of numerou...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/s12020-017-1418-y
更新日期:2018-05-01 00:00:00
abstract:PURPOSE:Non-functioning pituitary adenomas (NFPA) have a substantial impact on patients' health status, yet research on the extent of healthcare utilization and costs among these patients is scarce. The objective was to determine healthcare usage, associated costs, and their determinants among patients treated for an N...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/s12020-019-01847-7
更新日期:2019-05-01 00:00:00
abstract::Studies designed to evaluate the short-term effects of incretin-related drugs in subjects with cardiac disease are still preliminary. In patients with heart failure, two of five studies showed that glucagon-like peptide-1 (GLP-1) infusion was associated with an absolute increase in left ventricular ejection fraction (...
journal_title:Endocrine
pub_type: 杂志文章,评审
doi:10.1007/s12020-014-0175-4
更新日期:2014-09-01 00:00:00
abstract::Chronic stress can have a deleterious effect on the re-productive axis that, for females, is manifested in an increased incidence of infertility. However, gonadal steroids may, in turn, affect a female's response to stress as measured by activity within the limbic-hypothalamic-pituitary-adrenal (LHPA) axis. What is no...
journal_title:Endocrine
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1385/ENDO:26:2:089
更新日期:2005-03-01 00:00:00
abstract:PURPOSE:Histologic and pTNM classification of differentiated thyroid cancer (DTC) is mandatory to assess risk of relapse, risk of death, and radioactive iodine administration. The impact of an expert central review of external pathology reports has not yet been reported. METHODS:Monocentric retrospective study to eval...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/s12020-020-02531-x
更新日期:2020-10-31 00:00:00