Molecular identification of Sarcocystis lutrae (Apicomplexa: Sarcocystidae) in muscles of five species of the family Mustelidae.

Abstract:

:Carnivores usually act as definitive hosts of Sarcocystis species. However, the number of reports on sarcocyst formation in musculature of predators is on the increase. In the present study, muscle samples of 68 mustelids collected in Lithuania were examined for sarcocysts of Sarcocystis species. Sarcocysts were detected in diaphragm, tongue and limb muscles of ten animals (14.7%) but were not discovered in the heart. Based on 18S rDNA, 28S rDNA, cox1 and ITS1 sequence analysis, Sarcocystis lutrae was identified in three American minks (Neovison vison), two beech martens (Martes foina), three Eurasian badgers (Meles meles), one Eurasian otter (Lutra lutra) and one European polecat (Mustela putorius). The intraspecific variability of this Sarcocystis species was determined only in ITS1 region. Based on the phylogenetic analysis, no clear separation of S. lutrae by intermediate hosts or geographical locations was established. This paper represents the first identification of S. lutrae in the American mink, the beech marten and the European polecat. Current results indicate that S. lutrae is a common species in the muscles of various European mustelids.

journal_name

Parasitol Res

journal_title

Parasitology research

authors

Prakas P,Strazdaitė-Žielienė Ž,Rudaitytė-Lukošienė E,Servienė E,Butkauskas D

doi

10.1007/s00436-018-5880-0

subject

Has Abstract

pub_date

2018-06-01 00:00:00

pages

1989-1993

issue

6

eissn

0932-0113

issn

1432-1955

pii

10.1007/s00436-018-5880-0

journal_volume

117

pub_type

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