Quantitative real-time optical imaging of the tissue metabolic rate of oxygen consumption.

abstract：:Doxorubicin is a commonly used chemotherapeutic employed to treat multiple human cancers, including numerous sarcomas and carcinomas. Furthermore, doxorubicin possesses strong fluorescent properties that make it an ideal reagent for modeling drug delivery by examining its distribution in cells and tissues. However, wh...

journal_title：Journal of biomedical optics

authors： Carlson M,Watson AL,Anderson L,Largaespada DA,Provenzano PP

更新日期：2017-11-01 00:00:00

abstract：:Diffusion of two model drugs-benzyl nicotinate and ibuprofen-and the plasma macromolecule albumin across atherosclerotic rabbit aorta was studied ex vivo by attenuated total reflection-Fourier transform infrared (ATR-FTIR) imaging. Solutions of these molecules were applied to the endothelial surface of histological se...

journal_title：Journal of biomedical optics

authors： Palombo F,Danoux CB,Weinberg PD,Kazarian SG

更新日期：2009-07-01 00:00:00

abstract：:Raman spectroscopy on single, living epithelial cells captured in a laser trap is shown to have diagnostic power over colorectal cancer. This new single-cell technology comprises three major components: primary culture processing of human tissue samples to produce single-cell suspensions, Raman detection on singly tra...

journal_title：Journal of biomedical optics

authors： Zheng F,Qin Y,Chen K

更新日期：2007-05-01 00:00:00

abstract：:As molecular imaging moves towards lower detection limits, the elimination of endogenous background signals becomes imperative. We present a facile background-suppression technique that specifically segregates the signal from surface-enhanced Raman scattering (SERS)-active nanoparticles (NPs) from the tissue autofluor...

journal_title：Journal of biomedical optics

authors： Mallia RJ,McVeigh PZ,Veilleux I,Wilson BC

更新日期：2012-07-01 00:00:00

abstract：:The scattering of polarized light from a two layer scattering medium is investigated using Monte Carlo simulations. First order and normalized second order moments are used to analyze the spatial properties of the emerging light in different polarization states. Linearly and circularly polarized illumination is used t...

journal_title：Journal of biomedical optics

authors： Stockford IM,Morgan SP,Chang PC,Walker JG

更新日期：2002-07-01 00:00:00

abstract：:The objective of this work was to compare the accuracy of analyte concentration estimation when using transmission versus diffuse reflectance spectroscopy of a scattering medium. Monte Carlo ray tracing of light through the medium was used in conjunction with pure component absorption spectra and Beer-Lambert absorpti...

journal_title：Journal of biomedical optics

authors： Gardner CM

更新日期：2018-01-01 00:00:00

abstract：:Optical coherence tomography (OCT)-based elasticity imaging can map soft tissue elasticity based on speckle-tracking of elastic wave propagation using highly sensitive phase measurements of OCT signals. Using a fixed elastic wave source and moving detection, current imaging sequences have difficulty in reconstructing ...

journal_title：Journal of biomedical optics

authors： Hsieh BY,Song S,Nguyen TM,Yoon SJ,Shen TT,Wang RK,O'Donnell M

更新日期：2016-11-01 00:00:00

abstract：:Peripheral nerve injury in vivo promotes a regenerative growth in vitro characterized by an improved neurite regrowth. Knowledge of the conditioning injury effects on both morphology and mechanical properties of live sensory neurons could be instrumental to understand the cellular and molecular mechanisms leading to t...

journal_title：Journal of biomedical optics

authors： Benzina O,Szabo V,Lucas O,Saab MB,Cloitre T,Scamps F,Gergely C,Martin M

更新日期：2013-10-01 00:00:00

abstract：:We investigated the effect of thiazone, a widely used penetration enhancer, on in vitro porcine skin and muscle tissue by single-integrating sphere technique during optical clearing (OC) treatment. The results showed that thiazone induced an increase on the total transmittance of skin which led to a reduction in that ...

journal_title：Journal of biomedical optics

authors： Jin X,Deng Z,Wang J,Ye Q,Mei J,Zhou W,Zhang C,Tian J

更新日期：2016-10-01 00:00:00

abstract：:In order to understand the relationship between accommodation and vision quality, a custom-built ultra-long scan depth spectral domain optical coherence tomography (UL-SDOCT) and a Shack-Hartmann wavefront sensor (HSWFS) were combined. The resolution and scan depth of UL-SDOCT are 6 μm and 15.6 mm, respectively, which...

journal_title：Journal of biomedical optics

authors： Shi G,Wang Y,Yuan Y,Wei L,Lv F,Zhang Y

更新日期：2012-12-01 00:00:00

abstract：:Limited cutaneous systemic sclerosis (lcSSc) was formerly known as CREST syndrome in reference to the associated clinical features: calcinosis, Raynaud's phenomenon, esophageal dysfunction, sclerodactyly, and telangiectasias. The transforming growth factor beta has been identified as a major player in the pathogenic p...

journal_title：Journal of biomedical optics

authors： Barolet D

更新日期：2014-01-01 00:00:00

abstract：:We examine the morphological and chemical changes in the pulp chamber dentin after using endodontic agents by scanning electron microscopy (SEM), Fourier transform Raman spectroscopy (FT-Raman), and micro energy-dispersive x-ray fluorescence spectrometry (μEDXRF). Thirty teeth were sectioned exposing the pulp chamber ...

journal_title：Journal of biomedical optics

authors： Pascon FM,Kantovitz KR,Soares LE,Santo AM,Martin AA,Puppin-Rontani RM

更新日期：2012-07-01 00:00:00

abstract：:One of the prime limiting factors of optical imaging in biological applications is the diffusion of light by tissue, which prevents focusing at depths greater than the optical diffusion limit (typically ?1??mm). To overcome this challenge, wavefront shaping techniques that use a spatial light modulator (SLM) to correc...

journal_title：Journal of biomedical optics

authors： Hemphill AS,Tay JW,Wang LV

更新日期：2016-12-01 00:00:00

abstract：:We address the automatic differentiation of human tissue using multispectral imaging with promising potential for automatic visualization during surgery. Currently, tissue types have to be continuously differentiated based on the surgeon's knowledge only. Further, automatic methods based on optical in vivo properties ...

journal_title：Journal of biomedical optics

authors： Wisotzky EL,Uecker FC,Arens P,Dommerich S,Hilsmann A,Eisert P

更新日期：2018-05-01 00:00:00

abstract：:We interrogate the ability of free-space fluorescence tomography to image small animals in vivo using charge-coupled device (CCD) camera measurements over 360-deg noncontact projections. We demonstrate the performance of normalized dual-wavelength measurements that are essential for in-vivo use, as they account for th...

journal_title：Journal of biomedical optics

authors： Deliolanis NC,Dunham J,Wurdinger T,Figueiredo JL,Tannous BA,Ntziachristos V

更新日期：2009-05-01 00:00:00

abstract：:The specificity of molecular and functional photoacoustic (PA) images depends on the accuracy of the photoacoustic absorption spectroscopy. The PA signal is proportional to the product of the optical absorption coefficient and local light fluence; quantitative PA measurements of the optical absorption coefficient ther...

journal_title：Journal of biomedical optics

authors： Bauer AQ,Nothdurft RE,Erpelding TN,Wang LV,Culver JP

更新日期：2011-09-01 00:00:00

abstract：:Ophthalmic surgery may benefit from use of more precise fiber delivery systems during laser surgery. Some current ophthalmic surgical techniques rely on tedious mechanical dissection of tissue layers. In this study, chains of sapphire microspheres integrated into a hollow waveguide distal tip are used for erbium:YAG l...

journal_title：Journal of biomedical optics

authors： Hutchens TC,Darafsheh A,Fardad A,Antoszyk AN,Ying HS,Astratov VN,Fried NM

更新日期：2012-06-01 00:00:00

abstract：:Spectrally resolved two-photon excited autofluorescence imaging is used to distinguish different cell types and functional areas during dynamic processes in the living gut. Excitation and emission spectra of mucosal tissue and tissue components are correlated to spectra of endogenous chromophores. We show that selecti...

journal_title：Journal of biomedical optics

authors： Orzekowsky-Schroeder R,Klinger A,Martensen B,Blessenohl M,Gebert A,Vogel A,Hüttmann G

更新日期：2011-11-01 00:00:00

abstract：:For a long time, the antioxidative status in cattle has been discussed as an indicator for stress conditions resulting from disease or exertion. Until now, invasive approaches have been necessary to obtain blood samples or biopsy materials and gain insights into the antioxidative status of cattle. Due to these efforts...

journal_title：Journal of biomedical optics

authors： Klein J,Darvin ME,Müller KE,Lademann J

更新日期：2012-10-01 00:00:00

abstract：:We present the light scattering properties of individual human red blood cells (RBCs). We show that both the RBC static and dynamic scattering signals are altered by adenosine 5'-triphosphate (ATP)-driven membrane metabolic remodeling. To measure the light scattering signal from individual RBCs, we use diffraction pha...

journal_title：Journal of biomedical optics

authors： Park Y,Best-Popescu CA,Dasari RR,Popescu G

更新日期：2011-01-01 00:00:00

abstract：:The erratum concerns amendment of Section 2.5 of the published article. ...

journal_title：Journal of biomedical optics

authors： Hu XS,Arredondo MM,Gomba M,Confer N,DaSilva AF,Johnson TD,Shalinsky M,Kovelman I

更新日期：2020-05-01 00:00:00

abstract：:We have built an all-solid-state camera that is directly modulated at the pixel level for frequency-domain fluorescence lifetime imaging microscopy (FLIM) measurements. This novel camera eliminates the need for an image intensifier through the use of an application-specific charge coupled device design in a frequency-...

journal_title：Journal of biomedical optics

authors： Zhao Q,Schelen B,Schouten R,van den Oever R,Leenen R,van Kuijk H,Peters I,Polderdijk F,Bosiers J,Raspe M,Jalink K,Geert Sander de Jong J,van Geest B,Stoop K,Young IT

更新日期：2012-12-01 00:00:00

abstract：:Polarized Raman spectroscopy allows measurement of molecular orientation and composition and is widely used in the study of polymer systems. Here, we extend the technique to the extraction of quantitative orientation information from bone tissue, which is optically thick and highly turbid. We discuss multiple scatteri...

journal_title：Journal of biomedical optics

authors： Raghavan M,Sahar ND,Wilson RH,Mycek MA,Pleshko N,Kohn DH,Morris MD

更新日期：2010-05-01 00:00:00

abstract：:The Nikon C1 confocal laser scanning microscope is a relatively inexpensive and user-friendly instrument. We describe a straightforward method to convert the C1 for multiphoton microscopy utilizing direct coupling of a femtosecond near-infrared laser into the scan head and fiber optic transmission of emission light to...

journal_title：Journal of biomedical optics

authors： Mancuso JJ,Larson AM,Wensel TG,Saggau P

更新日期：2009-05-01 00:00:00

abstract：:Most reconstruction algorithms used in photoacoustic tomography do not account for the effects of acoustic attenuation on the recorded signals. For experimental measurements made in biological tissue, the frequency dependent acoustic attenuation causes high frequency components of the propagating photoacoustic waves t...

journal_title：Journal of biomedical optics

authors： Treeby BE

更新日期：2013-03-01 00:00:00

abstract：:We describe the protocol for an inexpensive and nondestructive optical reflectance assay for the measurement of biofilm formation. Reflectance data are obtained using an Ocean Optics (Dunedin, Florida) USB 2000 spectrometer with a polychromatic light source. A fiber optic cable is used both for illumination and collec...

journal_title：Journal of biomedical optics

authors： Broschat SL,Loge FJ,Peppin JD,White D,Call DR,Kuhn E

更新日期：2005-07-01 00:00:00

abstract：:Fluorescence lifetime imaging microscopy (FLIM) using multiphoton excitation techniques is now finding an important place in quantitative imaging of protein-protein interactions and intracellular physiology. Recent developments in multiphoton FLIM methods are reviewed and a novel multiphoton FLIM system using a streak...

journal_title：Journal of biomedical optics

authors： Krishnan RV,Masuda A,Centonze VE,Herman B

更新日期：2003-07-01 00:00:00

abstract：:Fourier domain (FD) techniques have increasingly gained attention in optical coherence tomography (OCT). This is primarily due to their demonstrated sensitivity of two to three orders of magnitude over conventional time-domain techniques. FDOCT images are subject to two primary sources of artifacts. First, a complex c...

journal_title：Journal of biomedical optics

authors： Davis AM,Choma MA,Izatt JA

更新日期：2005-11-01 00:00:00

abstract：:Serum Raman spectroscopy (RS) has previously shown potential in oral cancer diagnosis and recurrence prediction. To evaluate the potential of serum RS in oral cancer screening, premalignant and cancer-specific detection was explored in the present study using 328 subjects belonging to healthy controls, premalignant, d...

journal_title：Journal of biomedical optics

authors： Sahu AK,Dhoot S,Singh A,Sawant SS,Nandakumar N,Talathi-Desai S,Garud M,Pagare S,Srivastava S,Nair S,Chaturvedi P,Murali Krishna C

更新日期：2015-11-01 00:00:00

abstract：:Magnetomotive optical coherence tomography (MM-OCT) can be utilized to spatially localize the presence of magnetic particles within tissues or organs. These magnetic particle-containing regions are detected by using the capability of OCT to measure small-scale displacements induced by the activation of an external ele...

journal_title：Journal of biomedical optics

authors： Ahmad A,Kim J,Shemonski ND,Marjanovic M,Boppart SA

更新日期：2014-12-01 00:00:00