Abstract:
:The three dimensional ultrastructure of cells and tissues comes to light with tomography. There is an inherent tension between representing molecular detail at the highest possible resolution, on one hand, and visualizing spatial relations between large organelles or even neighboring cells in large volumes, on the other. Together with its advantages for pristine sample preservation, cryo-tomography brings particular constraints. A major challenge has been the restriction to specimens thinner than the typical cell. New imaging modalities are now available to extend cryo-tomography to thicker specimens: cryo-scanning transmission electron tomography (CSTET), soft X-ray tomography (SXT), and serial surface imaging using the focused ion beam-scanning electron microscope (FIB-SEM). Each one offers specific advantages so the optimal choice depends on priorities among resolution, composition, and volume.
journal_name
Curr Opin Microbioljournal_title
Current opinion in microbiologyauthors
Elbaum Mdoi
10.1016/j.mib.2018.01.001subject
Has Abstractpub_date
2018-06-01 00:00:00pages
155-161eissn
1369-5274issn
1879-0364pii
S1369-5274(17)30049-8journal_volume
43pub_type
杂志文章,评审abstract::The 21st century has seen the licensure of new anti-infective vaccines that have demonstrated their benefit for both individual and population (herd) protection. Despite this there are still many human pathogens for which no vaccine is available. As we learn more about these pathogens, and as technologies advance, mor...
journal_title:Current opinion in microbiology
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journal_title:Current opinion in microbiology
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journal_title:Current opinion in microbiology
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abstract::The obligatory intracellular bacterial pathogens Anaplasma and Ehrlichia infect leukocytes by hijacking host-cell components and processes. The type IV secretion system is up-regulated during infection. Among type IV secretion candidate substrates, an ankyrin repeat protein of Anaplasma phagocytophilum, AnkA, is deliv...
journal_title:Current opinion in microbiology
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journal_title:Current opinion in microbiology
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journal_title:Current opinion in microbiology
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journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
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journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
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journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
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journal_title:Current opinion in microbiology
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journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.mib.2017.11.010
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journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
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journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
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更新日期:2002-06-01 00:00:00
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journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.mib.2010.10.006
更新日期:2010-12-01 00:00:00
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journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
doi:10.1016/S1369-5274(99)80041-1
更新日期:1999-06-01 00:00:00
abstract::Haloalkaliphiles are double extremophilic organisms thriving both at high salinity and alkaline pH. Although numerous haloalkaliphilic representatives have been identified among Archaea and Bacteria over the past 15 years, the adaptations underlying their prosperity at haloalkaline conditions are scarcely known. A mul...
journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.mib.2015.05.003
更新日期:2015-06-01 00:00:00
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journal_title:Current opinion in microbiology
pub_type: 杂志文章
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journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.mib.2011.12.002
更新日期:2012-02-01 00:00:00
abstract::Aspergillus fumigatus is an opportunistic fungal pathogen that causes life-threatening infections in immunocompromised individuals. Siderophore-mediated iron acquisition has been shown to be essential for virulence. New studies have revealed that enzymes involved in siderophore biosynthesis and uptake are compartmenta...
journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.mib.2013.07.012
更新日期:2013-12-01 00:00:00
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journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.mib.2004.12.009
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journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
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更新日期:2014-12-01 00:00:00
abstract::Phagocytic cells, including macrophages, neutrophils and dendritic cells, are critical components of the innate immune response to bacterial pathogens such as Salmonella typhimurium. These cells can have several roles during the early stage of an infection including controlling bacterial replication and producing cyto...
journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.mib.2003.12.008
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abstract::Activation of professional phagocytes with the concomitant generation of oxidant species is a medullar innate immune process for the control of acute Trypanosoma cruzi infection. Recent data reinforce the hypothesis that parasites more prepared to deal with the host-oxidative assault are more efficient for the establi...
journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
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journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
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更新日期:2003-08-01 00:00:00
abstract::Schizosaccharomyces pombe is a useful model system for understanding many aspects of eukaryotic cell growth. Studies of S. pombe have identified novel genes that function in the regulation of iron homeostasis. In response to high levels of iron, Fep1 represses the expression of several genes involved in the acquisitio...
journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.mib.2013.07.007
更新日期:2013-12-01 00:00:00
abstract::The ability to control uptake across the mucosa and to protect the gut from harmful substances present in the lumen is defined as intestinal barrier function. Two routes are usually distinguished for transepithelial transport. The paracellular route allows the passage of ions and small molecules and is mainly regulate...
journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.mib.2013.12.003
更新日期:2014-02-01 00:00:00
abstract::The genomic revolution is fully upon us in 2006 and the pace of discovery is set to accelerate with the emergence of ultra-high-throughput sequencing technologies. Our complete genome collection of bacteria and archaea continues to grow in number and diversity, as genome sequencing is applied to an array of new proble...
journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.mib.2006.08.008
更新日期:2006-10-01 00:00:00
abstract::Sequestered from the action of complement, antibody and lytic peptides, phagosomal pathogens pose a unique problem for the innate immune system both in terms of detection and disposal. An immunologically induced 47-kDa (p47) GTPase family recruited to nascent phagosomes (PGs) has provided new insights into how vertebr...
journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
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更新日期:2005-02-01 00:00:00
abstract::In response to starvation, myxobacteria build multicellular fruiting bodies that contain many thousands of cells and that have particular species-specific shapes. To coordinate fruiting body development, the myxobacterial cells signal to each other. The timing of and cellular responses to these signals help to give fo...
journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
doi:10.1016/s1369-5274(98)80112-4
更新日期:1998-12-01 00:00:00
abstract::Bacterial cell division takes place almost entirely below the diffraction limit of light microscopy, making super-resolution microscopy ideally suited to interrogating this process. I review how super-resolution microscopy has advanced our understanding of bacterial cell division. I discuss the mechanistic implication...
journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.mib.2017.12.005
更新日期:2018-06-01 00:00:00