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Affinity of nucleotides for the active site of detergent-solubilized sarcoplasmic reticulum CaATPase.

Abstract：

:A dye displacement method was developed for determination of the affinities of compounds toward the active site of the detergent-solubilized sarcoplasmic reticulum CaATPase. Titration of the enzyme with 2',3'-O-(2,4,6-trinitrophenyl)-ADP resulted in a large absorbance difference in the visible spectrum. Subsequent addition of compounds which bind to the active site causes displacement of the dye; resulting absorbance changes were treated to gain dissociation constants for added ligands. The active site affinities of ATP, and nonhydrolyzable nucleotide analogues were determined. Both occupancy of the high affinity calcium site and added divalent cations have large influences on nucleotide affinity.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Coll RJ,Murphy AJ

doi

10.1016/s0006-291x(86)80546-0

subject

Has Abstract

pub_date

1986-07-31 00:00:00

pages

652-8

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(86)80546-0

journal_volume

138

pub_type

杂志文章

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