Molecular cloning and characterization of Cup a 4, a new allergen from Cupressus arizonica.

Abstract:

:Sensitization to Cupressaceae pollen has become one of the most important causes of pollinosis in Western countries during winter and early spring. However, the characterization of the extracts, the allergens involved and the cross-reactivity with other pollen sources still remain poorly studied; in the case of Cupressus arizonica only two allergens have been described so far. A new allergen from C. arizonica pollen, Cup a 4, was cloned and expressed in Escherichia coli as an N-terminally His-tag recombinant protein that was characterized biochemically, immunologically and by circular dichroism spectroscopy. The new allergen has high sequence identity with Prickly Juniper allergen Jun o 4 and contains four EF-hand domains. The recombinant protein has structural similarities with other calcium binding allergens such as Ole e 3, Ole e 8 and Phl p 7. Cup a 4 is expressed in mature pollen grains and shares antigenic properties with the recombinant form. Sera from 9.6% C. arizonica allergic patients contain specific IgE antibodies against recombinant Cup a 4.

authors

Pico de Coaña Y,Parody N,Fuertes MÁ,Carnés J,Roncarolo D,Ariano R,Sastre J,Mistrello G,Alonso C

doi

10.1016/j.bbrc.2010.09.079

subject

Has Abstract

pub_date

2010-10-22 00:00:00

pages

451-7

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(10)01776-6

journal_volume

401

pub_type

杂志文章
  • Regulation of HSF1-responsive gene expression by N-terminal truncated form of p73alpha.

    abstract::DNp73 is a transactivation domain (TAD)-truncated form of p73. The ability of DNp73alpha to regulate gene expression was examined using reporter assays with luciferase gene constructs. Among various promoter-regulated reporter genes tested, heat shock factor (HSF)-responsive gene expression was selectively activated b...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2004.03.124

    authors: Tanaka Y,Kameoka M,Itaya A,Ota K,Yoshihara K

    更新日期:2004-05-07 00:00:00

  • Separation of dolichol and dolichyl-P in microsomal and lysosomal compartments of hepatocytes.

    abstract::The distribution, labeling and interrelationship of microsomal and lysosomal dolichol and dolichyl-P in rat liver was investigated. After membrane induction with phenobarbital, N-nitrosodiethylamine and diethylhexylphthalate, the amount of microsomal and lysosomal dolichols are modulated independently. Liposomal label...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(83)80022-9

    authors: Edlund C,Chojnacki T,Dallner G

    更新日期:1983-09-30 00:00:00

  • Characterization of representative enzymes from a sulfate reducing bacterium implicated in the corrosion of steel.

    abstract::This communication reports the isolation, purification and characterization of key enzymes involved in dissimilatory sulfate reduction of a sulfate reducing bacterium classified as Desulfovibrio desulfuricans subspecies desulfuricans New Jersey (NCIMB 8313) (Ddd NJ). The chosen strain, originally recovered from a corr...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1996.0610

    authors: Pereira AS,Franco R,Feio MJ,Pinto C,Lampreia J,Reis MA,Calvete J,Moura I,Beech I,Lino AR,Moura JJ

    更新日期:1996-04-16 00:00:00

  • Evidence for a Ca2+ channel within the ryanodine receptor complex from cardiac sarcoplasmic reticulum.

    abstract::The solubilized [3H]ryanodine receptor from cardiac sarcoplasmic reticulum was centrifuged through linear sucrose gradients. A single peak of radioactivity with apparent sedimentation coefficient of approximately 30S specifically comigrated with a high molecular weight protein of apparent relative molecular mass appro...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(88)90613-4

    authors: Lai FA,Anderson K,Rousseau E,Liu QY,Meissner G

    更新日期:1988-02-29 00:00:00

  • Peroxynitrite-mediated oxidation of ferrous carbonylated myoglobin is limited by carbon monoxide dissociation.

    abstract::Peroxynitrite-mediated oxidation of ferrous nitrosylated myoglobin (Mb(II)-NO) involves the transient ferric nitrosylated species (Mb(III)-NO), followed by ()NO dissociation and formation of ferric myoglobin (Mb(III)). In contrast, peroxynitrite-mediated oxidation of ferrous oxygenated myoglobin (Mb(II)-O2) involves t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2007.09.053

    authors: Ascenzi P,Ciaccio C,Coletta M

    更新日期:2007-11-30 00:00:00

  • Deltoid human muscle mtDNA is extensively rearranged in old age subjects.

    abstract::Extra long PCR analysis of mitochondrial DNA (mtDNA) isolated from skeletal muscle of humans of different ages revealed three phenomena: (i) the amount of normal length mtDNA (16.5 kb) was progressively reduced with age, such that the cells of old age individuals (about 90 years) contained little, or undetectable amou...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1997.6251

    authors: Kovalenko SA,Kopsidas G,Kelso JM,Linnane AW

    更新日期:1997-03-06 00:00:00

  • Key components of COPI and COPII machineries are required for chikungunya virus replication.

    abstract::The infection of CHIKV is associated with cellular membranes; however whether early secretory pathways are involved in CHIKV replication remains unclear. In the present study, we have provided initial evidences that CHIKV requires both COPI and COPII for its replication. Small interfering RNAs against COPI components,...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2017.09.142

    authors: Zhang N,Zhang L

    更新日期:2017-11-25 00:00:00

  • Aggregation of marine sponge cells induced by Ca pulses, Ca ionophores, and phorbol esters proceeds in the absence of external Ca.

    abstract::Cells of the sponge Microciona prolifera dissociated in Ca,Mg-free sea water reaggregate upon addition of Ca, an observation classically attributed to the requirement for Ca of a species-specific aggregation factor. We now report that, unexpectedly, extracellular Ca is not required during aggregation; brief Ca pulses ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(86)90394-3

    authors: Dunham P,Weissmann G

    更新日期:1986-02-13 00:00:00

  • PI3K-delta mediates double-stranded RNA-induced upregulation of B7-H1 in BEAS-2B airway epithelial cells.

    abstract::Airway viral infection disturbs the health-related quality of life. B7-H1 (also known as PD-L1) is a coinhibitory molecule associated with the escape of viruses from the mucosal immunity, leading to persistent infection. Most respiratory viruses generate double-stranded (ds) RNA during replication. The stimulation of ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2013.04.082

    authors: Kan-o K,Matsumoto K,Asai-Tajiri Y,Fukuyama S,Hamano S,Seki N,Nakanishi Y,Inoue H

    更新日期:2013-05-31 00:00:00

  • TTX resistivity of Na+ channel in newt retinal neuron.

    abstract::We examined voltage-dependent, TTX-resistant Na+ channels of newt retina (nRNaCh) electrophysiologically. IC50-TTX value of nRNaCh is more than 20 microM. We determined partial cDNA sequences of nRNaCh restricted to TTX binding sites (SS2 regions of all four repeats). While the amino acid sequences of SS2 regions of r...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1997.7696

    authors: Kaneko Y,Matsumoto G,Hanyu Y

    更新日期:1997-11-26 00:00:00

  • 23Na NMR detects protection by glycine and alanine against hypoxic injury in the isolated perfused rat kidney.

    abstract::Protection against hypoxic injury by supraphysiological glycine and alanine concentrations was investigated in the isolated perfused rat kidney (IPRK). 23Na NMR detects consistent increases in total renal Na in IPRK during hypoxic perfusion. Increasing the concentration of glycine and alanine to 5 mM each produced a 3...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1994.2121

    authors: Endre ZH,Cowin GJ,Stewart-Richardson P,Cross M,Willgoss DA,Duggleby RG

    更新日期:1994-08-15 00:00:00

  • Diferric transferrin reduction stimulates the Na+/H+ antiport of HeLa cells.

    abstract::Proton release from HeLa cells is stimulated by external oxidants for the transplasmalemma electron transport enzymes. These oxidants, such as ferricyanide and diferric transferrin, also stimulate cell growth. We now present evidence that proton release associated with the reduction of ferricyanide and diferric transf...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(87)91344-1

    authors: Sun IL,Garcia-Cañero R,Liu W,Toole-Simms W,Crane FL,Morré DJ,Löw H

    更新日期:1987-05-29 00:00:00

  • Evidence for the presence of two homer 1 transcripts in skeletal and cardiac muscles.

    abstract::A family of proteins, Homers 1, 2 and 3, involved in activity-dependent control of signal transduction has been recently described in neurons [Xiao, B., Tu, C. J., Petralia, R. S., Yuan, J. P., Doan, A., Breder, C. D., Ruggiero, A., Lanahan, A. A., Wenthold, R. J., and Worley, P. F. (1998) Homer regulates the associat...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.2000.3948

    authors: Sandonà D,Tibaldo E,Volpe P

    更新日期:2000-12-20 00:00:00

  • Zinc coupling potentiates anti-HIV-1 activity of baicalin.

    abstract::Baicalin (BA) has been shown with anti-HIV-1 activity. Zinc is a nutrient element. The anti-HIV-1 activity of zinc complex of baicalin (BA-Zn) in vitro was studied and compared with the anti-HIV-1 activities between BA and BA-Zn in the present study. Our results suggested that BA-Zn has lower cytotoxicity and higher a...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2004.09.093

    authors: Wang Q,Wang YT,Pu SP,Zheng YT

    更新日期:2004-11-12 00:00:00

  • The effect of phytochrome action on the activity of cytosolic cholinesterase in oat cells.

    abstract::Cholinesterases in the oat cell were found to be distributed in the cell wall (50%) and cytoplasm (42%). Activity of the cytosolic enzyme was inhibited about 80% by 1 mM Ca2+. The enzyme activity was also inhibited by Mn2+, but no inhibition by Mg2+ was observed. Effects of red light and calcium ion on the enzyme acti...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(90)91448-2

    authors: Kim HY,Kim TI,Kim HK,Chae Q

    更新日期:1990-05-31 00:00:00

  • Calyculin A-induced neurite retraction is critically dependent on actomyosin activation but not on polymerization state of microtubules.

    abstract::Calyculin A (CL-A), a toxin isolated from the marine sponge Discodermia calyx, is a strong inhibitor of protein phosphatase 1 (PP1) and 2A (PP2A). Although CL-A is known to induce rapid neurite retraction in developing neurons, the cytoskeletal dynamics of this retraction have remained unclear. Here, we investigated t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2009.10.108

    authors: Inutsuka A,Goda M,Fujiyoshi Y

    更新日期:2009-12-25 00:00:00

  • Sphingomyelin potentiates chemotherapy of human cancer xenografts.

    abstract::We propose that one manifestation of altered sphingolipid metabolism within tumor cells may be a reduced sensitivity to anti-cancer therapies because of an inability to produce a sufficient apoptotic signal via sphingomyelin hydrolysis to ceramide. If so, then sphingomyelin administration could reverse this effect and...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.2000.2178

    authors: Modrak DE,Lew W,Goldenberg DM,Blumenthal R

    更新日期:2000-02-16 00:00:00

  • Immune responses with DNA vaccines encoded different gene fragments of severe acute respiratory syndrome coronavirus in BALB/c mice.

    abstract::To analyze the immune responses of DNA vaccine encoded different gene fragments of severe acute respiratory syndrome coronavirus (SARS-Cov), SARS-Cov gene fragments of membrane (M), nucleocapsid (N), spike a (Sa), and spike b (Sb) proteins were cloned into pcDNA3.1 (Invitrogen) vector to form plasmids pcDNAM, pcDNAN, ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2004.11.147

    authors: Wang Z,Yuan Z,Matsumoto M,Hengge UR,Chang YF

    更新日期:2005-02-04 00:00:00

  • Direct induction of hepatocyte-like cells from immortalized human bone marrow mesenchymal stem cells by overexpression of HNF4α.

    abstract::Hepatocytes from human bone marrow-derived mesenchymal stem cells (hBM-MSCs) are expected to be a useful source for cell transplantation. However, relatively low efficiency and repeatability of hepatic differentiation of human BM-MSCs remains an obstacle for clinical translation. Hepatocyte nuclear factor 4 alpha (HNF...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2016.08.026

    authors: Hu X,Xie P,Li W,Li Z,Shan H

    更新日期:2016-09-16 00:00:00

  • Salmon cartilage proteoglycan modulates cytokine responses to Escherichia coli in mouse macrophages.

    abstract::Proteoglycans (PGs) are complex glycohydrates, which are composed of core proteins and glycosaminoglycans and widely distributed in connective tissues and on the cell surface of mammalian tissues. We investigated the effect of PG extracted from salmon cartilage on cytokine responses to stimulation with heat-killed Esc...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2006.10.146

    authors: Sashinami H,Takagaki K,Nakane A

    更新日期:2006-12-29 00:00:00

  • Oncogenic virus-associated neoplasia: a role for cyclin D1 genotypes influencing the age of onset of disease?

    abstract::Cyclin D1 (CCND1) is a key regulatory protein at the G1/S checkpoint of the cell cycle. The purpose of our study was to assess the role of CCND1 genotypes influencing the age of onset of oncogenic virus-associated neoplasia. We conducted a hospital-based case-control study of 581 individuals, including 247 controls an...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2008.03.039

    authors: Catarino R,Pereira D,Breda E,Coelho A,Matos A,Lopes C,Medeiros R

    更新日期:2008-05-23 00:00:00

  • Expression of HIV-1 envelope glycoprotein alters cellular calmodulin.

    abstract::Removal of parts of a known calmodulin binding site at the C-terminus of HIV-1 envelope glycoprotein, gp160, can result in diminished infectivity. We investigated whether expression of full length gp160 would result in changes in intracellular calmodulin compared to expression of gp160 truncated to remove both known c...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1996.0034

    authors: Radding W,Pan ZQ,Hunter E,Johnston P,Williams JP,McDonald JM

    更新日期:1996-01-05 00:00:00

  • The GS domain of the transforming growth factor-beta type I receptor is important in signal transduction.

    abstract::Signal transduction by transforming growth factor-beta (TGF-beta) involves the formation of a heteromeric complex of two transmembrane serine/threonine kinase receptors, type I (T beta R-I) and type II (T beta R-II). In the region preceeding the kinase domain of T beta R-I there is a glycine- and serine-rich sequence,...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1995.1241

    authors: Franzén P,Heldin CH,Miyazono K

    更新日期:1995-02-15 00:00:00

  • Characterization of the genomic structure and promoter of the mouse NAD+-dependent 15-hydroxyprostaglandin dehydrogenase gene.

    abstract::The mouse NAD+-dependent 15-hydroxyprostaglandin dehydrogenase (15-PGDH) gene and its 5'-flanking region was cloned from a 129 mouse ES bacteriophage P1 genomic library. The gene contains 7 exons and 6 introns and is 11.3 kb in length. The transcription initiation site was mapped at 35 bases upstream from the ATG star...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1997.6831

    authors: Matsuo M,Ensor CM,Tai HH

    更新日期:1997-06-27 00:00:00

  • Altered expression of the fragile histidine triad gene in primary gastric adenocarcinomas.

    abstract::Genomic alterations and abnormal expression of the fragile histidine triad (FHIT) gene in gastric carcinomas were examined to determine whether the FHIT gene is actually a frequent target for alteration during gastric carcinogenesis. To correlate DNA and RNA lesions of the FHIT gene with the effect on FHIT protein exp...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.2001.5038

    authors: Lee SH,Kim WH,Kim HK,Woo KM,Nam HS,Kim HS,Kim JG,Cho MH

    更新日期:2001-06-15 00:00:00

  • POU-domain genes in planarian Dugesia japonica: the structure and expression.

    abstract::In fresh water planarian Dugesia japonica, two genes encoding POU-domain proteins, DjPOU1 and DjPOU2, have been identified by polymerase chain reaction (PCR). Their POU-domains are strikingly similar to class III POU-domains in mammals, frog, insects and nematode. The full-length cDNA of DjPOU1 has been cloned and seq...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1993.1571

    authors: Orii H,Agata K,Watanabe K

    更新日期:1993-05-14 00:00:00

  • Multi-wavelength immunoassays using surface plasmon-coupled emission.

    abstract::We describe a new method for multi-wavelength immunoassays using surface plasmon-coupled emission (SPCE). This phenomenon is coupling of excited fluorophores with a nearby thin metal film, in our case silver, resulting in strongly directional emission into the underlying glass substrate. The angle at which the radiati...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2003.12.010

    authors: Matveeva E,Malicka J,Gryczynski I,Gryczynski Z,Lakowicz JR

    更新日期:2004-01-16 00:00:00

  • Cytotoxicity of a new IMP dehydrogenase inhibitor, benzamide riboside, to human myelogenous leukemia K562 cells.

    abstract::COMPARE computer program suggested that benzamide riboside, BR, 3-(1-deoxy-beta-D-ribofuranosyl)benzamide, should have a similar mechanism of action as that of tiazofurin, an inhibitor of IMP dehydrogenase (IMPDH). This hypothesis was tested in K562 cells in culture. BR was cytotoxic to K562 cells with an IC50 of 2 mi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(05)81591-8

    authors: Jayaram HN,Gharehbaghi K,Jayaram NH,Rieser J,Krohn K,Paull KD

    更新日期:1992-08-14 00:00:00

  • Targeting carbonic anhydrase to treat diabetic retinopathy: emerging evidences and encouraging results.

    abstract::Diabetic retinopathy (DR) is the leading cause of vision loss among working-age populations in developed countries. Current treatment options are limited to tight glycemic, blood pressure control and destructive laser surgery. Carbonic anhydrases (CAs) are a group of enzymes involving in the rapid conversion of carbon...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章,评审

    doi:10.1016/j.bbrc.2009.10.031

    authors: Weiwei Z,Hu R

    更新日期:2009-12-18 00:00:00

  • Differential effects of two-pore channel protein 1 and 2 silencing in MDA-MB-468 breast cancer cells.

    abstract::Two-pore channel proteins, TPC1 and TPC2, are calcium permeable ion channels found localized to the membranes of endolysosomal calcium stores. There is increasing interest in the role of TPC-mediated intracellular signaling in various pathologies; however their role in breast cancer has not been extensively evaluated....

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2016.06.127

    authors: Jahidin AH,Stewart TA,Thompson EW,Roberts-Thomson SJ,Monteith GR

    更新日期:2016-09-02 00:00:00