Permanent cell lines that show temperature-dependent expression of adenovirus virus-associated RNA.

Abstract:

:Temperature-sensitive COS cells, clone E540, have been stably transformed at a restrictive temperature with plasmid pVA1, which contains the adenovirus type 5 virus-associated (VA) genes in addition to the Neor marker. Transformed cell clones, named EVA cells, contained adenovirus DNA in an integrated form while grown at restrictive temperature but accumulated up to 100 to 200 copies of the input plasmid per cell after temperature shift down. Concomitant with this gene amplification, an accumulation of VA RNA was observed, reaching average concentrations of 10(4) to 10(5) copies per cell. The VA RNA synthesized in EVA cells is functional, as judged by inhibition of in vitro eucaryotic initiation factor-2 phosphorylation and enhancement of reporter gene expression. These EVA cell lines may be of use to study the mechanism of VA RNA function in the absence of adenovirus infection.

journal_name

J Virol

journal_title

Journal of virology

authors

Martínez C,de la Luna S,Peláez F,Lopez-Turiso A,Valcárcel J,de Haro C,Ortín J

doi

10.1128/JVI.63.12.5445-5450.1989

subject

Has Abstract

pub_date

1989-12-01 00:00:00

pages

5445-50

issue

12

eissn

0022-538X

issn

1098-5514

journal_volume

63

pub_type

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