Abstract:
:N(pro) is a multifunctional autoprotease unique to pestiviruses. The interacting partners of the N(pro) protein of classical swine fever virus (CSFV), a swine pestivirus, have been insufficiently defined. Using a yeast two-hybrid screen, we identified poly(C)-binding protein 1 (PCBP1) as a novel interacting partner of the CSFV N(pro) protein and confirmed this by coimmunoprecipitation, glutathione S-transferase (GST) pulldown, and confocal assays. Knockdown of PCBP1 by small interfering RNA suppressed CSFV growth, while overexpression of PCBP1 promoted CSFV growth. Furthermore, we showed that type I interferon was downregulated by PCBP1, as well as N(pro). Our results suggest that cellular PCBP1 positively modulates CSFV growth.
journal_name
J Viroljournal_title
Journal of virologyauthors
Li D,Li S,Sun Y,Dong H,Li Y,Zhao B,Guo D,Weng C,Qiu HJdoi
10.1128/JVI.02807-12subject
Has Abstractpub_date
2013-02-01 00:00:00pages
2072-80issue
4eissn
0022-538Xissn
1098-5514pii
JVI.02807-12journal_volume
87pub_type
杂志文章abstract::The severe acute respiratory syndrome coronavirus (SARS-CoV) encodes numerous accessory proteins whose importance in the natural infection process is currently unclear. One of these accessory proteins is set apart by its function in the context of a related murine hepatitis virus (MHV) infection. SARS-CoV protein 6 in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02406-07
更新日期:2008-07-01 00:00:00
abstract::It was demonstrated that Epstein-Barr virus (EBV)-encoded small RNAs (EBERs) were nonessential for B-lymphocyte growth transformation. We revisited this issue by producing a large quantity of EBER-deleted EBV by using an Akata cell system. Although the EBER-deleted virus efficiently infected B lymphocytes, its 50% tra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.7.4298-4307.2005
更新日期:2005-04-01 00:00:00
abstract::The human cytomegalovirus (HCMV) major immediate-early (IE) proteins share an 85-amino-acid N-terminal domain specified by exons 2 and 3 of the major IE region, UL122-123. We have constructed IE Delta30-77, a recombinant virus that lacks the majority of IE exon 3 and consequently expresses smaller forms of both IE1 72...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.12.7438-7452.2005
更新日期:2005-06-01 00:00:00
abstract::Signal transducers and activators of transcription (STATs) relay signals from activated cell surface receptors directly to the nucleus. Previously, a protein required for T-cell transformation by the DNA tumor virus herpesvirus saimiri (HVS) and designated tyrosine kinase interacting protein (Tip-484) was shown to int...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.9.6677-6682.1997
更新日期:1997-09-01 00:00:00
abstract::The infected cell polypeptide 4 (ICP4) of herpes simplex virus 1 (HSV-1) is a regulator of viral transcription that is required for productive infection. Since viral genes are transcribed by cellular RNA polymerase II (RNA pol II), ICP4 must interact with components of the pol II machinery to regulate viral gene expre...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00385-11
更新日期:2011-06-01 00:00:00
abstract::Simian virus 40 mutants lacking sequences in the late leader region are viable but produce smaller plaques than does wild-type virus. Within three passages at low multiplicities of infection, virus stocks of several such mutants accumulated variants that synthesized an altered form of the major virion protein, VP1, ha...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.10.3190-3198.1987
更新日期:1987-10-01 00:00:00
abstract::Mutations at amino acids 143, 148, and 155 in HIV-1 integrase (IN) define primary resistance pathways in subjects failing raltegravir (RAL)-containing treatments. Although each pathway appears to be genetically distinct, shifts in the predominant resistant virus population have been reported under continued drug press...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06618-11
更新日期:2012-07-01 00:00:00
abstract::The ORF3 protein of hepatitis E virus (HEV) is a multifunctional protein important for virus replication. The ORF3 proteins from human, swine, and avian strains of HEV contain a conserved PXXP amino acid motif, resembling either Src homology 3 (SH3) cell signaling interaction motifs or "late domains" involved in host ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06711-11
更新日期:2012-05-01 00:00:00
abstract::Highly pathogenic influenza A viruses (IAV) from avian hosts were first reported to directly infect humans 20 years ago. However, such infections are rare events, and our understanding of factors promoting or restricting zoonotic transmission is still limited. One accessory protein of IAV, PB1-F2, was associated with ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00425-18
更新日期:2018-05-14 00:00:00
abstract::Infection of cells with herpes simplex virus type 1 (HSV-1) induces high levels of deoxypyrimidine triphosphatase. The majority of the enzyme activity is found in infected cell nuclei. A similar activity is induced by HSV type 2 (HSV-2) which, in contrast to the HSV-1 enzyme, fractionates to more than 99% in the solub...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.43.3.935-942.1982
更新日期:1982-09-01 00:00:00
abstract::PM2 DNA was prepared with different superhelical densities (sigma) in order to examine the relationship betweenn supercoiling and the occurrence of a region(s) of unpaired bases in this DNA. A previous study showed that CH3HgOH reacts with native superhelical PM2 DNA more rapidly than the nicked form II. This evaluati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.18.1.195-204.1976
更新日期:1976-04-01 00:00:00
abstract::Functional defects in cytotoxic CD8(+) T cell responses arise in chronic human viral infections, but the mechanisms involved are not well understood. In mice, CD4 cell-mediated interleukin-21 (IL-21) production is necessary for the maintenance of CD8(+) T cell function and control of persistent viral infections. To in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02030-10
更新日期:2011-01-01 00:00:00
abstract::The configuration of latent varicella-zoster virus (VZV) DNA was analyzed by PCR. Template DNA for both internal and terminal VZV primers was present in a 1:1 ratio in ganglionic DNA, compared with a 15:1 ratio in DNA extracted from VZV virions, indicating that the VZV genomic termini are adjacent in latently infected...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.12.8151-8154.1995
更新日期:1995-12-01 00:00:00
abstract::Influenza viruses often evade host immunity via antigenic drift and shift despite previous influenza virus infection and/or vaccination. Vaccines that match circulating virus strains are needed for optimal protection. Development of a universal influenza virus vaccine providing broadly cross-protective immunity will b...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00120-13
更新日期:2013-05-01 00:00:00
abstract::The required activities of CD4(+) T cells and antibody against the virally encoded oncoprotein simian virus 40 (SV40) Tag have previously been demonstrated by our laboratory to be mediators in achieving antitumor responses and tumor protection through antibody-dependent cell-mediated cytotoxicity (ADCC). In this study...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01512-09
更新日期:2010-01-01 00:00:00
abstract::The virion proteins encoded by the avian retroviral pol gene (reverse transcriptase and endonuclease) are formed by the proteolytic processing of a gag-pol fusion protein precursor. Recent studies have predicted that the avian sarcoma-leukosis virus pol precursor protein undergoes a previously undetected processing ev...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.2.528-533.1988
更新日期:1988-02-01 00:00:00
abstract::Human papillomavirus (HPV) type 16 E6 (16E6) is an oncogenic, multifunctional nuclear protein that induces p53 degradation and perturbs normal cell cycle control, leading to immortalization and transformation of infected keratinocytes and epithelial cells. Although it is unclear how 16E6 disrupts the epigenetic profil...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.24.13232-13247.2003
更新日期:2003-12-01 00:00:00
abstract::The infection of cells which belong to the B-cell lineage is thought to be the primary event leading to the phenotypic and functional alterations seen in the murine AIDS (M. Huang, C. Simard, D. Kay, and P. Jolicoeur, J. Virol. 65:6562-6571, 1991). Using in situ hybridization, we studied the time course of the anatomi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.3.1903-1912.1994
更新日期:1994-03-01 00:00:00
abstract::Retroviral DNA integration requires the activity of at least one viral protein, the integrase (IN) protein. We cloned and expressed the integrase gene of feline immunodeficiency virus (FIV) in Escherichia coli as a fusion to the malE gene and purified the IN fusion protein by affinity chromatography. The protein is ac...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.3.1468-1474.1994
更新日期:1994-03-01 00:00:00
abstract::To determine whether the tripartite leader is required for efficient translation in adenovirus-infected cells at late times of infection, we constructed recombinant adenoviruses containing the influenza virus nucleocapsid protein (NP) gene expressed under the control of the adenovirus major late promoter (MLP). We cho...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.5.1606-1616.1988
更新日期:1988-05-01 00:00:00
abstract::Recently, novel arenaviruses were found in snakes with boid inclusion body disease (BIBD); these form the new genus Reptarenavirus within the family Arenaviridae. We used next-generation sequencing and de novo sequence assembly to investigate reptarenavirus isolates from our previous study. Four of the six isolates an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01112-15
更新日期:2015-08-01 00:00:00
abstract::Current human immunodeficiency virus type 1 (HIV-1) envelope vaccine candidates elicit high antibody binding titers with neutralizing activity against T-cell line-adapted but not primary HIV-1 isolates. Serum antibodies from these human vaccine recipients were also found to be preferentially directed to linear epitope...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.6.4319-4330.1997
更新日期:1997-06-01 00:00:00
abstract::Interleukin-10 (IL-10) suppresses the maturation and cytokine production of dendritic cells (DCs), key regulators of adaptive immunity, and prevents the activation and polarization of naïve T cells towards protective gamma interferon-producing effectors. We hypothesized that human cytomegalovirus (HCMV) utilizes its v...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.16.8720-8731.2004
更新日期:2004-08-01 00:00:00
abstract::Although the envelope gene of human immunodeficiency virus type 1 shows considerable strain variability, cysteine residues of the envelope protein are strongly conserved, suggesting that they are important to the envelope structure. We constructed and analyzed mutants of a biologically active molecular clone of human ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.5.2250-2259.1990
更新日期:1990-05-01 00:00:00
abstract:UNLABELLED:The HIV-1 glycoprotein 41 promotes fusion of the viral membrane with that of the target cell. Structural, biochemical, and biophysical studies suggest that its membrane-proximal external region (MPER) may interact with the HIV-1 membrane and induce its disruption and/or deformation during the process. Howeve...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02151-14
更新日期:2014-11-01 00:00:00
abstract::Retroviral capsid assembly can occur by either of two distinct morphogenic processes: in type C viruses, the capsid assembles and buds at the plasma membrane, while in type B and D viruses, the capsid assembles within the cytoplasm and is then transported to the plasma membrane for budding. We have previously reported...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.7.5431-5437.1999
更新日期:1999-07-01 00:00:00
abstract:UNLABELLED:TRIM5α proteins are a potent barrier to the cross-species transmission of retroviruses. TRIM5α proteins exhibit an ability to self-associate at many levels, ultimately leading to the formation of protein assemblies with hexagonal symmetry in vitro and cytoplasmic assemblies when expressed in cells. However, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01134-14
更新日期:2014-08-01 00:00:00
abstract::During herpes simplex virus (HSV) latency, most viral genes are silenced, with the exception of one region of the genome encoding the latency-associated transcript (LAT). This long noncoding RNA was originally described as having a role in enhancing HSV-1 reactivation. However, subsequent evidence showing that the LAT...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00812-18
更新日期:2018-07-31 00:00:00
abstract::Mother-to-child transmission can occur in utero, mainly intrapartum and postpartum in case of breastfeeding. In utero transmission is highly restricted and results in selection of viral variant from the mother to the child. We have developed an in vitro system that mimics the interaction between viruses, infected cell...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.10.4780-4791.2001
更新日期:2001-05-01 00:00:00
abstract::Influenza A virus infection can arrest autophagy, as evidenced by autophagosome accumulation in infected cells. Here, we report that this autophagosome accumulation can be inhibited by amantadine, an antiviral proton channel inhibitor, in amantadine-sensitive virus infected cells or cells expressing influenza A virus ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00576-15
更新日期:2015-10-14 00:00:00