Abstract:
:Ionic currents were recorded from Xenopus oocytes injected with RNA isolated from chick or mouse brain. Three currents were studied: a rapid tetrodotoxin-sensitive Na+ current (Ina), an early outward K+ current sensitive to 4-aminopyridine (IA), and an inward current activated by the excitatory amino acid receptor agonist kainate. Oligonucleotides (60-80 bases long) complementary to rat brain Na+ channel sequences were prehybridized to chick brain RNA. These DNA sequences, upon injection into oocytes, specifically inhibited expression of INa relative to IA and the kainate-induced current in a dose-dependent manner. By contrast, prehybridization of oligonucleotides complementary to sequences either from the Drosophila Shaker locus (which codes for an early K+ current in Drosophila muscle) or from a homologous clone from mouse brain did not block the expression of the early outward K+ current induced in the oocytes by mRNA from chick or mouse brain. This method provides a convenient means for testing the functional role of cloned DNA species.
journal_name
Neuronjournal_title
Neuronauthors
Lotan I,Volterra A,Dash P,Siegelbaum SA,Goelet Pdoi
10.1016/0896-6273(88)90153-5subject
Has Abstractpub_date
1988-12-01 00:00:00pages
963-71issue
10eissn
0896-6273issn
1097-4199pii
0896-6273(88)90153-5journal_volume
1pub_type
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