Abstract：
:Traditional studies of neuroanatomical connections require injection of tracer compounds into living brains, then histology of the postmortem tissue. Here, we describe and validate a compound that reveals neuronal connections in vivo, using MRI. The classic anatomical tracer CTB (cholera-toxin subunit-B) was conjugated with a gadolinium-chelate to form GdDOTA-CTB. GdDOTA-CTB was injected into the primary somatosensory cortex (S1) or the olfactory pathway of rats. High-resolution MR images were collected at a range of time points at 11.7T and 7T. The transported GdDOTA-CTB was visible for at least 1 month post-injection, clearing within 2 months. Control injections of non-conjugated GdDOTA into S1 were not transported and cleared within 1-2 days. Control injections of Gd-Albumin were not transported either, clearing within 7 days. These MR results were verified by classic immunohistochemical staining for CTB, in the same animals. The GdDOTA-CTB neuronal transport was target specific, monosynaptic, stable for several weeks, and reproducible.
journal_name
Neuronjournal_title
Neuronauthors
Wu CW,Vasalatiy O,Liu N,Wu H,Cheal S,Chen DY,Koretsky AP,Griffiths GL,Tootell RB,Ungerleider LGdoi
10.1016/j.neuron.2011.03.010subject
Has Abstractpub_date
2011-04-28 00:00:00pages
229-43issue
2eissn
0896-6273issn
1097-4199pii
S0896-6273(11)00204-2journal_volume
70pub_type
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