Abstract:
:The leaky heme mutants G204, G216, and G214 are shown to accumulate exogenous sterols. Unlike hem mutants which have complete blocks in the heme pathway, these strains do not require ergosterol, methionine, or unsaturated fatty acids for growth. The addition of aminolevulinic acid to the growth medium inhibited sterol uptake in G204 96% but had only a slight effect on sterol uptake by strains G214 and G216. Sterol uptake in all three strains was inhibited 83-94% when cells were grown in the presence of hematin. Sterol analysis of these strains grown in the presence and absence of either aminolevulinic acid or hematin revealed that saturation of the cell membrane with ergosterol was not responsible for the dramatic decrease in sterol uptake. These results suggest that sterol uptake by yeast cells is controlled by heme, and explain the non-viability of yeast strains that are heme competent and auxotrophic for sterols.
journal_name
Steroidsjournal_title
Steroidsauthors
Shinabarger DL,Keesler GA,Parks LWdoi
10.1016/0039-128x(89)90035-4subject
Has Abstractpub_date
1989-03-01 00:00:00pages
607-23issue
3-5eissn
0039-128Xissn
1878-5867pii
0039-128X(89)90035-4journal_volume
53pub_type
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