Analysis of progesterone in unextracted serum: a method using danazol [17 alpha-pregn-4-en-20-yno(2, 3-d) isoxazol-17-ol] a blocker of steroid binding to proteins.

abstract：:A radioimmunoassay for the quantitation of the sum of free, glucuronidated and urine is described. The method is reliable and accurate. Using this method, urinary excretion of 16 alpha-hydroxyestrone was determined in normal men, premenopausal women, and postmenopausal women. The values were compared to the urinary ex...

journal_title：Steroids

authors： Naganuma H,Hershcopf RJ,Michnovicz JJ,Miyairi S,Bradlow HL,Fishman J

更新日期：1989-01-01 00:00:00

abstract：:The four diastereomeric 3,16-diacetates 6, 9, 10 and 11 were prepared from gitoxin 1 by the routes shown in Schemes 1 and 2 and tested for inotropic activity in the isolated guinea-pig atrial preparation. In line with earlier findings in the digitoxigenin series, derivatives with a 3 alpha-acetoxy function, viz 9 and ...

journal_title：Steroids

authors： Humber DC,Jones PS,Phillipps GH

更新日期：1985-01-01 00:00:00

abstract：:Formestane (F, androst-4-en-4-ol-3,17-dione) is an irreversible aromatase inhibitor with the ability to suppress the estrogen production from anabolic steroids. Consequently, F is mentioned on the World Anti-Doping Agency (WADA) prohibited list and because studies have shown that F is produced endogenously in small am...

journal_title：Steroids

authors： Polet M,Van Renterghem P,Van Gansbeke W,Van Eenoo P

更新日期：2013-11-01 00:00:00

abstract：:In addition to using radioimmunoassays for the determination of estrogens and other steroids, the possibility of using mass fragmentography for analysis was investigated. t-Butyldimethylsilyl chloride was selected as a reagent for derivatisation because it forms rather stable silylethers. In all the mass-spectra obrai...

journal_title：Steroids

authors： Ballhorn L,Mueller WF,Korte F

更新日期：1979-04-01 00:00:00

abstract：:Glucocorticoid receptor binding was compared in liver cytosol preparations from spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats using homologous displacement of [3H]dexamethasone. At 5 degrees C, there was no difference in receptor binding affinity or concentration between strains for dexamethasone, ...

journal_title：Steroids

authors： Panarelli M,Holloway CD,Barr AB,Fraser R,Kenyon CJ

更新日期：1995-01-01 00:00:00

abstract：:Dioscorea panthaica is a traditional Chinese medicinal herb used in the treatment of various physiological conditions, including cardiovascular disease, gastropathy and hypertension. Steroidal saponins (SS) are the main active ingredients of this herb and have effects on myocardial ischemia and cancer. The phytochemic...

journal_title：Steroids

authors： Wang W,Zhao Y,Jing W,Zhang J,Xiao H,Zha Q,Liu A

更新日期：2015-03-01 00:00:00

abstract：:The metabolism of tamoxifen is being redefined in the light of several important pharmacological observations. Recent studies have identified 4-hydroxy N-desmethyltamoxifen (endoxifen) as an important metabolite of tamoxifen necessary for antitumor actions. The metabolite is formed through the enzymatic product of CYP...

journal_title：Steroids

authors： Jordan VC

更新日期：2007-11-01 00:00:00

abstract：:A first assay based on stable isotope dilution/gas chromatography-mass spectrometry (ID/GC-MS) has been developed for plasma 11-deoxycortisol (Reichstein's compound S), the leading hormonal marker of 11beta-hydroxylase deficiency. A suitable internal standard being unavailable, we synthesized dideuterated 11-deoxycort...

journal_title：Steroids

authors： Wudy SA,Hartmann M,Homoki J

更新日期：2002-09-01 00:00:00

abstract：:A radio-gas chromatographic method has been devised for the estimation of 11-oxotestosterone (17beta-hydroxyandrost-4-ene-3, 11-dione) in fish plasma samples which provides an independent means of validating the radioimmunoassay described earlier. Estimates of the concentration of 11-oxotestosterone in a sample of mal...

journal_title：Steroids

authors： Simpson TH,Wright RS

更新日期：1978-05-01 00:00:00

abstract：:The non-genomic action of steroid hormones regulates a wide variety of cellular responses including regulation of ion transport, cell proliferation, migration, death and differentiation. In order to achieve such plethora of effects steroid hormones utilize nearly all known signal transduction pathways. One of the key ...

journal_title：Steroids

authors： Alzamora R,Harvey BJ

更新日期：2008-10-01 00:00:00

abstract：:Dehydroepiandrosterone (DHEA) fatty acyl esters once incorporated in high density lipoprotein (HDL) induce a stronger vasodilatory response in rat mesenteric arteries ex vivo compared to native HDL. We studied the role of HDL receptor, scavenger receptor class B, type 1 (SR-B1), as well as estrogen and androgen recept...

journal_title：Steroids

authors： Paatela H,Vihma V,Jauhiainen M,Mervaala E,Tikkanen MJ

更新日期：2011-03-01 00:00:00

abstract：:Human mammary medullary carcinoma cells (passages 16 to 21) were cultured for 2 days to allow for attachment, followed by 6 days of culture in either fetal calf serum, human cord blood, human amniotic fluid, or growth factors in the presence or absence of purified human alpha-fetoprotein (AFP). When growth factors wer...

journal_title：Steroids

authors： Leal JA,Gangrade BK,Kiser JL,May JV,Keel BA

更新日期：1991-05-01 00:00:00

abstract：:The first convenient chemical synthesis of 7alpha-hydroxyandrost-4-ene-3,17-dione is reported. Androsta-4,6-diene3, 17-dione was converted into its 6alpha, 7alpha-epoxy-derivative; reduction of the epoxide with aluminum amalgam gave 7alpha-hydroxyandrost-4-ene-3,17-dione. This reducing agent is more efficient than chr...

journal_title：Steroids

authors： Hossain AM,Kirk DN,Mitra G

更新日期：1976-05-01 00:00:00

abstract：:Testosterone (17 beta -hydroxy-4-androsten-3-one ; T) and dihydrotestosterone (17 beta -hydroxy-5 alpha -androstan-3-one ; DHT) concentrations were determined by radioimmunoassay in different fetal tissues taken from male guinea-pigs. Androgen concentrations were maximal in the components of the Wolffian duct (vas def...

journal_title：Steroids

authors： Rigaudière N

更新日期：1981-08-01 00:00:00

abstract：:A series of clickable bile acid-nucleosides conjugates linked directly or via amino acid linker were synthesized, and characterized by spectroscopic techniques such as 1H NMR, 13C NMR, FT-IR, HRMS and HPLC. The synthesized compounds 6a-p were screened for their in vitro anticancer property against a panel of three can...

journal_title：Steroids

authors： Agarwal DS,Siva Krishna V,Sriram D,Yogeeswari P,Sakhuja R

更新日期：2018-11-01 00:00:00

abstract：:This study examines the distribution of 5 alpha-reductase and 3 alpha(beta)-hydroxysteroid dehydrogenase activities throughout the intact hyperplastic prostate gland and relate these measurements to the fibromuscular/epithelial composition and to the gross glandular morphology. The relative capacities of the stroma an...

journal_title：Steroids

authors： Habib FK,Beynon L,Chisholm GD,Busuttil A

更新日期：1983-01-01 00:00:00

abstract：:By the conventional methods of gas liquid chromatography (GLC) as well as mass spectrometry, 3beta,7alpha-dihydroxychol-5-en-24-oic acid (delta5-acid), a key intermediate of chenodeoxycholic acid biogenesis and its metabolic by-product, 3alpha,7alpha-dihydroxychol-4-en-24-oic acid (delta4-acid) have not yet been ident...

journal_title：Steroids

authors： Ikeda M,Yamasaki K

更新日期：1978-07-01 00:00:00

abstract：:20-Hydroxyecdysterone - (2β,3β,5β,22R)-2,3,14,20,22,25-hexahydroxycholest-7-en-6-one was isolated in satisfactory yield using ethanol extraction from the aerial part of Silene wolgensis (Hornem.) Otth; sometimes Silene wolgensis (Willd.) Bess. ex Spreng. The complexation of the phytoecdysteroid with β-cyclodextrin was...

journal_title：Steroids

authors： Temirgaziyev BS,Kučáková K,Baizhigit YA,Jurášek M,Džubák P,Hajdúch M,Dolenský B,Drašar PB,Tuleuov BI,Adekenov SM

更新日期：2019-07-01 00:00:00

abstract：:The purpose of this study was to investigate the influence of plasma steroid-binding proteins on androgen metabolism in intact leukocytes prepared from normal male and female blood samples. Leukocyte preparations were incubated for 24 h at 37 degrees C with either labeled or unlabeled testosterone (T), 5 alpha-dihydro...

journal_title：Steroids

authors： Déchaud H,Goujon R,Claustrat F,Boucherat M,Pugeat M

更新日期：1995-02-01 00:00:00

abstract：:Nine new steroidal glycosides, named as stauntosides C-K (2, 5, 7-10, 13, 14, and 16), along with seven known compounds (1, 3, 4, 6, 11, 12, and 15) were isolated from the 95% ethanol extract of the roots of Cynanchum stauntonii. The structures of these new compounds were elucidated on the basis of extensive spectrosc...

journal_title：Steroids

authors： Yu JQ,Deng AJ,Qin HL

更新日期：2013-01-01 00:00:00

abstract：:Polycystic ovary syndrome (PCOS) is associated with multiple risk factors for cardiovascular disease (CVD), including insulin resistance, type 2 diabetes mellitus, obesity, hypertension, and dyslipidemia. In addition, hyperandrogenism may contribute to the pathogenesis of CVD, independent of obesity and insulin resist...

journal_title：Steroids

authors： Choi YS,Yang HI,Cho S,Jung JA,Jeon YE,Kim HY,Seo SK,Lee BS

更新日期：2012-11-01 00:00:00

abstract：:Fermentation of mesterolone (1) with Cunninghamella blakesleeana yielded four new metabolites, 1α-methyl-1β,11β,17β-trihydroxy-5α-androstan-3-one (2), 1α-methyl-7α,11β,17β-trihydroxy-5α-androstan-3-one (3), 1α-methyl-1β,6α,17β-trihydroxy-5α-androstan-3-one (4) and 1α-methyl-1β,11α,17β-trihydroxy-5α-androstan-3-one (5)...

journal_title：Steroids

authors： Ahmad MS,Zafar S,Bibi M,Bano S,Atia-Tul-Wahab,Atta-Ur-Rahman,Iqbal Choudhary M

更新日期：2014-04-01 00:00:00

abstract：:A series of novel 5α, 8α-endoperoxide steroidal hybrid derivatives containing isatin or indole substituents on the C-17 side chain were synthesized and characterized. The preliminary anti-proliferative activity of the compounds against HepG2, MCF-7, HT-29 and HeLa cell lines were investigated. Compounds 7g and 7l disp...

journal_title：Steroids

authors： Li H,Wang H,Wang J,Lin Y,Ma Y,Bu M

更新日期：2020-01-01 00:00:00

abstract：:It has now been more than 30 years since the laboratory verification of the localization of estrogen in certain animal tissues. Much has been learned since that time regarding the details of this process, including the presence of specific receptors for these hormones in target tissues, the mechanism of ligand binding...

journal_title：Steroids

authors： Cummins CH

更新日期：1993-06-01 00:00:00

abstract：:From the aerial parts of Euphorbia sogdiana Popov, obtusifoliol (1) and two related steroids (2-3) have been isolated and characterized along with a known cycloartane derivative (4). The chemical structure of the obtusifoliol-related compounds, obtained by 1D and 2D NMR, and MS measurements, have been determined as: 3...

journal_title：Steroids

authors： Aghaei M,Yazdiniapour Z,Ghanadian M,Zolfaghari B,Lanzotti V,Mirsafaee V

更新日期：2016-11-01 00:00:00

abstract：:Synthetic transformations of steroids for drug discovery and improvement of drug effectiveness have been an important part of modern medicinal chemistry and pharmaceutical sciences. Pentacyclic triterpenoids, being represented in the nature by various structures and biogenetically related to steroids, can largely expa...

journal_title：Steroids

authors： Tolmacheva IA,Nazarov AV,Eroshenko DV,Grishko VV

更新日期：2018-12-01 00:00:00

abstract：:Inhibition of 5alpha-reduction of testosterone by an anti-androgen TSAA-291 (16beta-ethyl-17beta-hydroxy-4-estren-3-one) was studied in rat ventral prostates and the metabolic conversion of 3H-TSAA-291 was examined both in vitro and in vivo. In the in vitro experiment using nuclear 5alpha-reductase of the prostate, 5a...

journal_title：Steroids

authors： Sudo K,Yoshida K,Akinaga Y,Nakayama R

更新日期：1981-07-01 00:00:00

abstract：:Homogenates from human myometrium with an added NADPH regenerating system and ATP were incubated with 4-(14)C progesterone. Six metabolites were identified: 5alpha-pregnane-3, 20-dione, 5beta-pregnane-3, 20-dione, 3alpha-hydroxy-4-pregnen-20-one, 3beta hydroxy-4-pregnen-20-one. 20alpha-hydroxy-4-pregnen-3-one and 20be...

journal_title：Steroids

authors： Junkermann H,Runnebaum B,Lisboa BP

更新日期：1977-07-01 00:00:00

abstract：:3 alpha-Hydroxysteroid dehydrogenase (3 alpha HSD) is one of the main enzymes involved in the metabolism of the active androgen, dihydrotestosterone (DHT). 3 alpha HSD catalyzes the reversible reduction of DHT to 5 alpha-androstane-3 alpha, 17 beta-diol (3 alpha DIOL). The equilibrium of 3 alpha HSD reductive and oxid...

journal_title：Steroids

authors： Pirog EC,Collins DC

更新日期：1994-04-01 00:00:00

abstract：:The abundant expression of progesterone receptors (PR) in human meningiomas is well established. It is unknown, however, how PR expression is regulated, especially since estrogen receptors (ER) are virtually absent in these tumors. At the mRNA level, ER splice variants occur in meningioma but these appear not to be in...

journal_title：Steroids

authors： Blankenstein MA,Verheijen FM,Jacobs JM,Donker TH,van Duijnhoven MW,Thijssen JH

更新日期：2000-10-01 00:00:00