Abstract:
:Activating transcription factor 2 (ATF2) is a member of the cAMP response element binding protein family that heterodimerizes and activates other transcription factors involved in stress and DNA damage responses, growth, differentiation and apoptosis. ATF2 has been investigated as a potential carcinogenic biomarker in certain types of cancer, such as melanoma. However, its function and clinical significance in non-small cell lung cancer (NSCLC) has not been well studied. Therefore, the present study aimed to analyze the association between ATF2/phosphorylated (p)-ATF2 expression and NSCLC malignant behavior, and discuss its clinical significance. Reverse transcription-quantitative polymerase chain reaction and western blotting were used to detect the expression of ATF2 in NSCLC cell lines and fresh NSCLC tissue samples. In addition, immunohistochemistry (IHC) was performed to identify the location and expression of ATF2 and p-ATF2 (threonine 71) in paraffin-embedded sections of NSCLC and adjacent normal tissue. The results demonstrated that ATF2 was markedly overexpressed in the NSCLC cells and significantly overexpressed in the fresh NSCLC tissues compared with the control cells and samples (86 paraffin-embedded tissue sections), respectively (P<0.01). Further data demonstrated that ATF2 expression levels were significantly increased in tumor tissues compared to normal tissues and ATF2 was located in the cytoplasm and nucleus. ATF2 expression was closely associated with adverse clinical characteristics such as TNM stage (P=0.002), tumor size (P=0.018) and metastasis (P=0.027). In addition, nuclear p-ATF2 staining was positive in 65/86 samples of NSCLC. Furthermore, the Kaplan-Meier analysis indicated that patients with high levels of ATF2 and p-ATF2 expression had a significantly shorter overall survival compared with patients exhibiting a low expression (P<0.01 and P<0.05, respectively). Subsequent in vitro experiments revealed that cell growth decreased following knockdown of ATF2 expression using RNA interference, indicating that ATF2 may suppress cell proliferation. Taken together, the results of the present study demonstrated that ATF2 and p-ATF2 were significantly overexpressed in NSCLC tissues, and ATF2 and p-ATF2 overexpression predicted significantly worse outcomes for patients with NSCLC.
journal_name
Oncol Lettjournal_title
Oncology lettersauthors
You Z,Zhou Y,Guo Y,Chen W,Chen S,Wang Xdoi
10.3892/ol.2015.3922subject
Has Abstractpub_date
2016-01-01 00:00:00pages
760-766issue
1eissn
1792-1074issn
1792-1082pii
OL-0-0-3922journal_volume
11pub_type
杂志文章相关文献
Oncology Letters文献大全abstract::Osteosarcoma (OS) is the most common form of bone malignancy in children and adolescents. A class of molecules known as microRNAs (miRNAs) have been routinely associated in the development and progression of OS. The present study was centered on the less well-known miRNA, miRNA (miR)-150, and its role in OS was invest...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.5709
更新日期:2017-04-01 00:00:00
abstract::Non-small cell lung cancer (NSCLC) is the leading cause of death in smokers and the most common cause for cancer mortality in both males and females in the United States. Predisposition of this malignancy to distant metastasis leads to poor prognosis; therefore, it is urgent to discover novel therapeutic agents for me...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.7668
更新日期:2018-03-01 00:00:00
abstract::The anticancer effect of Scutellaria baicalensis extract has recently become a topic of interest. In this study, the anticancer effects and underlying mechanisms of wogonoside, the main constituent of Scutellaria baicalensis, were investigated in a human hepatocellular carcinoma (HCC) cell line in vitro. The effects o...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.3408
更新日期:2015-09-01 00:00:00
abstract::Cisplatin resistance has been a major factor limiting its clinical use as a chemotherapy drug. The present study aimed to investigate whether SET and MYND domain-containing protein 3 (SMYD3), a histone methyltransferase closely associated with tumors can affect the sensitivity of tumors to cisplatin chemotherapy. Real...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2020.11465
更新日期:2020-05-01 00:00:00
abstract::Multiple myeloma is a type of malignancy, which affects the plasma cells of the bone marrow. Recent studies have found that malignant plasma cells may express urokinase plasminogen activator (uPA) and uPA receptor (uPAR), and that initiation of proteolytic events by this system contributes to the process of invasion a...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.3613
更新日期:2015-10-01 00:00:00
abstract::Pancreatic cancer remains among the most lethal cancers, despite ongoing advances in treatment for all stages of the disease. Disease prevention represents another opportunity to improve patient outcome, with metabolic syndrome and its components, such as diabetes, obesity and dyslipidemia, having been recognized as m...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.5572
更新日期:2017-03-01 00:00:00
abstract::A previous study reported the decreased expression of long non-coding RNA mortal obligate RNA transcript (lncRNA MORT) in 16 types of cancer, while the functionality of lncRNA MORT in cancer biology remains unknown. Therefore, the present study was conducted to characterize the functionality of lncRNA MORT in prostate...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.10711
更新日期:2019-10-01 00:00:00
abstract::2',4'-dihydroxy-6'-methoxychalcone (cardamonin) is a natural compound with anti-proliferative effects on several cancer types including nasopharyngeal carcinoma. The effects of cardamonin on melanoma cells are unknown. The present study investigated the anti-proliferative effect of cardamonin on human melanoma cell li...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.11242
更新日期:2020-02-01 00:00:00
abstract::The radiological features of pulmonary sclerosing hemangioma (PSH) and pulmonary hamartoma are poorly specified. Thus, the present study aimed to compare and analyze the characteristics of fluorodeoxyglucose positron emission tomography/computed tomography (18F-FDG PET/CT) in PSH versus pulmonary hamartoma. 18F-FDG PE...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8660
更新日期:2018-07-01 00:00:00
abstract::Patients with chronic inflammatory bowel disease have an increased risk of colorectal cancer, and the differentiation between neoplastic and inflammatory lesions often poses a clinical dilemma. The aim of the present study was to investigate whether diffusion-weighted (DW) magnetic resonance (MR) enterography with ult...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.10441
更新日期:2019-08-01 00:00:00
abstract::The long non-coding RNA (lncRNA), RP11-480I12.5 is one of the most dysregulated lncRNAs, which is believed to contribute to the progression of cervical carcinoma (CC); however, the exact function of RP11-480I12.5 in human CC remains unknown. The present study aimed to investigate the function and underlying molecular ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.11120
更新日期:2020-01-01 00:00:00
abstract::Expression of the transcription factor hypoxiainducible factor 1 (HIF-1) plays a key role in cellular adaptation to hypoxia, particularly in relation to tumour angiogenesis. Expression of the HIF-1α subunit is responsive to changes in oxygen levels. Overexpression of HIF-1α has been reported to be associated with a po...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2013.1185
更新日期:2013-04-01 00:00:00
abstract::Aberrant expression of microRNAs (miRNAs) contributes to the progression and outcomes of several types of tumor, while circulating miRNAs have been reported to act as biomarkers for several types of cancer. To identify specific circulating miRNAs associated with multiple myeloma (MM), a miRNA microarray analysis was u...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.9128
更新日期:2018-09-01 00:00:00
abstract::Recently, a number of microRNAs (miRNAs) have been reported to play different roles in human cancers, including colorectal cancer (CRC). However, the specific role of miR-758 has not been clarified in CRC. Therefore, the aim of the present study was to explore the role of miR-758 in CRC. RT-qPCR and western blot analy...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2020.11516
更新日期:2020-06-01 00:00:00
abstract::In the majority of acute promyelocytic leukemia (APL) cases, translocons produce a promyelocytic leukemia protein-retinoic acid receptor α (PML-RARα) fusion gene. Studies have reported that neutrophil elastase (NE) cleaves bcr-1-derived PML-RAα in early myeloid cells, leaving only the nuclear localization signal (NLS)...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.5706
更新日期:2017-04-01 00:00:00
abstract::Special AT-rich sequence binding protein 1 (SATB1) is a nuclear factor that functions as a global chromatin organizer to regulate gene expression. Recent studies have suggested an oncogenic role of SATB1 in breast cancer. However, the role of SATB1 in gastric cancer, especially in regulating the malignant phenotypes, ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.681
更新日期:2012-07-01 00:00:00
abstract::Despite the efficacy of combination chemotherapy with arsenic trioxide (ATO), interferon α (IFN) and zidovudine (AZT) for adult T cell leukemia/lymphoma (ATL), the precise mechanism underlying this combination treatment effect is unknown. In the present study, ATO/IFN/AZT was examined in an ATL leukemic cell line (S1T...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8771
更新日期:2018-07-01 00:00:00
abstract::Evidence-based guidelines for the prevention of surgical site infection (SSI) have been published by the U.S. Centers for Disease Control and Prevention (CDC). According to these guidelines, a wound should usually be covered with a sterile dressing for 24 to 48 h when a surgical incision is closed primarily. However, ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.687
更新日期:2012-07-01 00:00:00
abstract::The seven-amino acid truncated (7ND) protein is an N-terminal deletion mutant of monocyte chemoattractant protein-1 (MCP-1) and it functions as a dominant-negative inhibitor. 7ND and wild-type MCP-1 form a heterodimer, which binds to MCP-1 receptors and inhibits monocyte chemotaxis. In the present study, the 7ND prote...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8308
更新日期:2018-05-01 00:00:00
abstract::The present study aimed to investigate the clinical characteristics, diagnosis and treatment of accessory breast cancer, and contribute valuable information regarding this rare tumour to the current literature, ultimately facilitating the development of improved treatment strategies. The present study reported the cas...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.3388
更新日期:2015-09-01 00:00:00
abstract::Euchromatic histone-lysine N-methyltransferase (G9A), the primary histone methyltransferase for histone H3 Lys9, has been identified to be upregulated in numerous types of cancer. The aim of the present study was to analyze the clinical significance of G9A, and preliminarily explore its function in hepatocellular carc...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8572
更新日期:2018-06-01 00:00:00
abstract::The basic helix-loop-helix transcription factor AP4 (TFAP4) gene serves an important function in the genesis and progression of tumors. However, few studies to date have defined the role of this gene in colorectal cancer (CRC). The aim of the present study was to assess the expression of TFAP4 in CRC and its impact on...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8290
更新日期:2018-05-01 00:00:00
abstract::A number of studies have revealed the usefulness of multimodal imaging in gliomas. Although the results have been heavily affected by the method used for region of interest (ROI) design, the most discriminatory method for setting the ROI remains unclear. The aim of the present study was to determine the most suitable ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8319
更新日期:2018-05-01 00:00:00
abstract::In patients with diffuse large B-cell lymphoma (DLBCL) classified as high-intermediate risk or high risk using the International Prognosis Index, the efficacy of high-dose chemotherapy combined with upfront autologous stem cell transplantation (HDT/ASCT) remains controversial in the rituximab era. In the present study...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.6589
更新日期:2017-09-01 00:00:00
abstract::MicroRNA (miR)-29a has been associated with carcinogenesis in humans; however, its functional significance in esophageal squamous cell carcinoma (ESCC) is yet to be determined. In the present study, the expression of miR-29a was markedly downregulated in ESCC tissue and the ESCC TE-1 cell line, compared with normal es...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2014.2678
更新日期:2015-01-01 00:00:00
abstract::Approximately 34-86% of neoplasms of the salivary glands are located in the parotid gland and parotidectomy is the first-line treatment for parotid gland tumors. Frey syndrome and cosmetic deformity are common complications experienced by patients following parotidectomy and the sternocleidomastoid muscle flap (SCMF) ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2013.1179
更新日期:2013-04-01 00:00:00
abstract::Significant antitumor activity of Momordica anti-human immunodeficiency virus protein of 30 kDa (MAP30) purified from Momordica charantia has been the subject of previous research. However, the effective mechanism of MAP30 on malignant glioma cells has not yet been clarified. The aim of the present study was to invest...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8073
更新日期:2018-04-01 00:00:00
abstract::Imiquimod is an imidazoquinoline immune response modifier that is used in antiviral and antiallergic creams. Combination therapy using transcutaneous imiquimod and oral sorafenib was previously demonstrated to reduce the tumor burden of renal cell carcinoma growing cutaneously in a mouse model. In the present study, a...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.6235
更新日期:2017-07-01 00:00:00
abstract::Special AT-rich sequence binding protein 1 (SATB1) has regulatory effects on gene expression and appears to play an important role in tumor progression. The present study screened the promoter region of the SATB1 gene for polymorphisms, evaluated the corresponding haplotypes regarding alterations in promoter activity ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2014.1983
更新日期:2014-06-01 00:00:00
abstract::Natural dietary components have become the subject of an increasing amount of interest due to the side effects of anticancer treatment. Pterostilbene, an analog of resveratrol, is primarily found in grapes, and has been suggested to exert antioxidant and anticancer effects in different tumor types. The present study a...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.10499
更新日期:2019-08-01 00:00:00