Abstract：
:Previous work has shown that purified rat macrophages lack both accessory activity for T lymphocyte responses to mitogens and stimulatory activity in a mixed leukocyte reaction, in marked contrast to the potent activity of dendritic cells. This study was designed to re-evaluate macrophages as accessory cells by treating various cell preparations with either silica or L-leucine methyl ester, which have been reported to be toxic to macrophages, and then determining the effect of the treated cells on responses to the mitogens, sodium periodate or concanavalin A. These studies indicated that treatment with L-leucine methyl ester failed to kill rat macrophages or dendritic cells, whereas silica was specifically toxic for rat macrophages. The studies therefore focused on silica. Co-culturing mitogen-treated lymph node cells with silica over a wide range of concentrations had no effect on responses. The same results were obtained if mitogen-treated lymphocytes were enriched with lymph node macrophages and dendritic cells and then co-cultured with silica. Preparations containing both macrophages and dendritic cells were incubated with silica for 24 h to ensure the death of virtually all macrophages; upon the addition of mitogen-treated lymphocytes, the macrophage-depleted accessory cells induced vigorous proliferative responses. Peritoneal exudate cells showed variable, but low accessory activity that increased after incubation with silica. Elimination of more than 90% of the macrophages from peritoneal exudate cells, as determined by staining for non-specific esterase, failed to eliminate this accessory activity. Taken together, these findings confirm and extend the conclusion that rat macrophages lack or have exceedingly low accessory activity.
journal_name
Immunobiologyjournal_title
Immunobiologyauthors
Bowers WE,Ruhoff MS,Goodell EM,Stoltenborg JKdoi
10.1016/S0171-2985(88)80052-4subject
Has Abstractpub_date
1988-02-01 00:00:00pages
179-94issue
3eissn
0171-2985issn
1878-3279pii
S0171-2985(88)80052-4journal_volume
176pub_type
杂志文章相关文献
IMMUNOBIOLOGY文献大全abstract：:Tuberculosis due to Mycobacterium tuberculosis infection is a leading cause of death worldwide. Recognition of this pathogen is crucial for the activation of innate and adaptive immune responses. Nucleotide-binding oligomerization domain (Nod)1 and Nod2 are cytoplasmic receptors that can detect unique muropeptides of ...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/j.imbio.2015.07.020
更新日期：2016-01-01 00:00:00
abstract：:Dendritic cells capture and process antigen and present it to T lymphocytes in the lymphoid organs. Dendritic cells of the skin, including epidermal Langerhans cells, langerin(+) and langerin(negative) dermal dendritic cells are ideally positioned to take up pathogens that enter the body through the skin or vaccines t...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/j.imbio.2010.05.014
更新日期：2010-09-01 00:00:00
abstract：:Exposure of resident peritoneal macrophages or thioglycollate-induced macrophages (TG-Mø) to influenza or Sendai virus-infected spleen cell culture supernatants (MAS) resulted in macrophage activation. When normal resident macrophages were used as effector cells, both infected P815 and L929 cells were lysed in the pre...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/S0171-2985(84)80023-6
更新日期：1984-05-01 00:00:00
abstract：:A glycoprotein from human erythrocyte membranes has been found to inhibit lysis of target cells by the attack-phase components C5-C9 from human complement. The inhibiting molecule was purified and identified as glycophorin A. Thus, glycophorin A may have a regulatory function in the lytic complement attack on isologou...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/S0171-2985(83)80053-9
更新日期：1983-08-01 00:00:00
abstract：:Intestinal epithelial cells produce cytokines in response to bacterial peptidoglycan (PGN), which is detected by several classes of pattern-recognition receptors (PRRs) as peptidoglycan recognition proteins (PGlyRPs), Toll-like receptor 2 (TLR2) and NOD receptors. All types of PGlyRPs recognize bacterial peptidoglycan...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/j.imbio.2011.10.013
更新日期：2012-04-01 00:00:00
abstract：:IFN-γ produced during viral infections activates the IFN-γ receptor (IFNGR) complex for STAT1 transcriptional activity leading to expression of Interferon Regulatory Factors (IRF). Simultaneous activation of TBK/IKKε via TLR3 during viral infections activates the transcription factor IRF3. Together these transcription...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/j.imbio.2019.04.004
更新日期：2019-07-01 00:00:00
abstract：:IgE-mediated food allergies are an important cause of life-threatening hypersensitivity reactions. Orally administered peanut antigens mixed with the mucosal adjuvant cholera toxin (CT) induce a strong peanut extract (PE)-specific serum IgE response that is correlated with T-helper type 1 (Th1) and type 2 (Th2)-like T...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/j.imbio.2010.02.004
更新日期：2010-12-01 00:00:00
abstract：:The major target organ of systemic infection with the intracellular bacterium Listeria monocytogenes is the liver, to where inflammatory leukocytes are rapidly recruited. We determined by reverse transcriptase polymerase chain reaction the early chemokine response in the liver after systemic infection of mice with lis...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/S0171-2985(98)80066-1
更新日期：1998-07-01 00:00:00
abstract：:The Fc gamma RIII receptor (CD16) has been described on natural killer cells and a small subset of T lymphocytes. CD16+bright lymphocytes represent the typical population of peripheral blood CD3- NK cells. In these studies in addition to CD16+bright NK cells Fc gamma RIII expressing cytotoxic T lymphocytes in peripher...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/S0171-2985(11)80315-3
更新日期：1992-06-01 00:00:00
abstract：:Blood dendritic cell precursors (DCps) are identified as mononuclear leukocytes expressing HLA-DR but lacking the characteristic antigens associated with T cells (CD3), NK cells (CD16 and CD56) and B cells (CD 19). Dendritic cell precursors are distinguished from monocytes by their lack of expression of CD64 rather th...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/S0171-2985(00)80053-4
更新日期：2000-05-01 00:00:00
abstract：:The alarming rise of obesity and type 2 diabetes (T2D) has put a tremendous strain on global healthcare systems. Over the past decade extensive research has focused on the role of macrophages as key mediators of inflammation in T2D. The inflammatory environment in the obese adipose tissue and pancreatic β-cell islets ...
journal_title：Immunobiology
pub_type： 杂志文章,评审
doi：10.1016/j.imbio.2016.08.011
更新日期：2017-10-01 00:00:00
abstract：:Fifty-nine patients with metastatic malignant melanoma were entered into a phase II trial of recombinant alpha-2 interferon given in a dosage of 10 million IU/M2 subcutaneously three times per week for one year. Forty-five of these were evaluable for response. Of five evaluable patients with ocular primaries, none res...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/S0171-2985(86)80109-7
更新日期：1986-09-01 00:00:00
abstract：:The history of immunotherapy for tuberculosis is briefly reviewed, and the early appreciation of the importance of secreted antigens, common mycobacterial antigens and stress proteins is noted. The methods by which Mycobacterium vaccae strain NCTC 11659 was selected for special attention, and results of some of the pi...
journal_title：Immunobiology
pub_type： 历史文章,杂志文章
doi：10.1016/S0171-2985(11)80462-6
更新日期：1994-10-01 00:00:00
abstract：:Acute lung injury (ALI) is an acute inflammatory disorder. Toll-like receptor-4 (TLR-4) and Stanniocalcin -1 (STC-1) had roles in lung endothelial protection. This study aims to assess TLR-4 and SCT-1 genes expressions in peripheral blood of ALI patients. Total RNA was extracted from peripheral blood of 48 subjects (2...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/j.imbio.2019.09.003
更新日期：2019-11-01 00:00:00
abstract：:We investigated the mRNA expression of inducible nitric oxide synthase (iNOS) and the production of nitric oxide (NO) in human T cell lymphotropic virus type I (HTLV-I) p40tax-transfected U937 cells, a human monoblast cell line. Transfection of HTLV-I p40tax U937 cells induced up-regulation of iNOS mRNA expression and...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/s0171-2985(97)80083-6
更新日期：1997-11-01 00:00:00
abstract：:The course of legionella pneumonia in guinea pigs after infection with various quantities of virulent L. pneumophila serogroup 1 organisms by aerosol exposure was investigated. The clinical course, histopathological characteristics, manifestations in the lungs and clearance of the legionella organisms from the lungs a...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/S0171-2985(87)80104-3
更新日期：1987-12-01 00:00:00
abstract：:Cryptococcus neoformans is a pathogenic fungus causing life threatening infections in humans. The present in vitro study aimed to investigate the opsonizing properties of a well characterized serum ficolin (rat ficolin-A), a member of carbohydrate-recognition molecules of the innate immune system, in the defence again...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/j.imbio.2012.06.006
更新日期：2013-04-01 00:00:00
abstract：:The anticonvulsant drug phenytoin, in less than cytotoxic concentrations, caused significant reductions in Ig secretion by unstimulated or EBV-stimulated normal MNC, as measured by PFC or secretion of Ig into the culture medium. Isotype-specific LBL varied in their sensitivity, the secretion of IgA (1 line) and IgG (3...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/S0171-2985(85)80094-2
更新日期：1985-09-01 00:00:00
abstract：:Pregnancy is known to induce a transient depression of maternal cell-mediated immunity, to prevent rejection of the fetus, while at the same time it keeps adequate maternal host defense mechanisms to fight infection. Presently, the aim of this paper was to investigate a possible endocrine and immunologic alteration ob...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/j.imbio.2014.09.005
更新日期：2015-01-01 00:00:00
abstract：:Using irradiation bone marrow chimeras to analyze restriction specificity of helper T cells, we found that recipient H-2 type dictated the H-2 type which the T cells recognize as self (adaptive differentiation). T cells from (H-2b----H-2k) chimeras cooperate with non-T cells bearing Iak to generate a vigorous PFC resp...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/S0171-2985(85)80054-1
更新日期：1985-02-01 00:00:00
abstract：INTRODUCTION:The interaction between the immune system and malignant diseases is a proven key target for cancer therapy. We describe an innovative 3D cell culture system comprising both immune and cancer cells to evaluate their interaction and immune cell infiltration to provide an innovative in vitro screening of immu...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/j.imbio.2015.12.010
更新日期：2016-05-01 00:00:00
abstract：BACKGROUND:An association between Tako-Tsubo cardiomyopathy (TTC) and underlying malignancies has been observed, suggesting that TTC might be the consequence of paraneoplastic phenomena. This study investigates the presence of autoantibodies against cardiomyocytes as well as adrenergic (β1, β2) and muscarinic (M2) rece...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/j.imbio.2018.11.005
更新日期：2019-03-01 00:00:00
abstract：:The process of thymopoiesis is tightly regulated by a series of selection events which ensure that only functional T-lymphocytes directed against foreign antigens are exported into the periphery. The adaptive immune response largely depends on the regulation of thymocyte development, and thymocytes which fail selectio...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/j.imbio.2007.05.003
更新日期：2007-01-01 00:00:00
abstract：:Investigating the expression of lipoteichoic acid (LTA) from Listeria monocytogenes, we found two distinct structural variants of LTA (LTA1 and LTA2) using NMR and MS technology. While both LTA consisted of a poly-glycerophosphate backbone (differing in length) bound via a disaccharide to a diacyl-glycerol moiety, one...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/j.imbio.2010.03.008
更新日期：2011-01-01 00:00:00
abstract：:The innate immune system recognizes pathogen-associated molecular patterns (PAMP) to cope with evolving infections. Toll-like receptors (TLRs) play a pivotal role in recognition of PAMPs. In the course of infection not a single but rather a full panel of different microbial components interacts with distinct TLRs simu...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1078/0171-2985-00189
更新日期：2002-10-01 00:00:00
abstract：:Interleukin-6 (IL-6) induced increased, leukocyte and platelet counts on around day 20 when it was administered into [BALB/c-->C3H/He] bone marrow chimeras from day 1 to day 12. Increased leukocyte counts and hemoglobin (Hb) levels were also observed at around day 60 and from day 41 to 80, respectively. On the other h...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/S0171-2985(11)80607-8
更新日期：1994-06-01 00:00:00
abstract：:The heavy-chain variable regions (VH) from 14F7 MAb, an IgG1 antibody specific for GM3(NeuGc) ganglioside, and its anti-idiotype, the 4G9 MAb, were cloned and sequenced. Comparison with previously reported sequences showed that VH 14F7 belongs to the J558(VHI) gene family and that it is highly mutated. VH 4G9 belongs ...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/j.imbio.2006.08.005
更新日期：2007-01-01 00:00:00
abstract：:The mechanisms of tolerance induction occurring in the course of allergen-specific immunotherapy have not been elucidated in full detail. Our study aimed to characterize high zone tolerance in mouse models of type I allergy and of allergic airway inflammation induced by subcutaneous sensitization of mice with high dos...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/j.imbio.2014.10.022
更新日期：2015-04-01 00:00:00
abstract：:High Mphi:T cell ratios suppress the immune response to the retroviral superantigen Mls by IFNgamma-triggered production of the arg- and trp-consuming enzymes iNOS and IDO. Attempts to reverse suppression by treatment with pro-inflammatory cytokines revealed that IL-6 improved the T cell response to Mls and the pro-he...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/j.imbio.2009.01.015
更新日期：2010-01-01 00:00:00
abstract：:Heat shock proteins (Hsps) or stress proteins are highly conserved molecules and expressed in all cell types under stressful conditions like heat, cold, hypoxia and infections. The objective of the present study was to determine the effect of dengue virus infection on relative expression of stress proteins and their r...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/j.imbio.2008.11.010
更新日期：2009-01-01 00:00:00
