Abstract:
:IFN-γ produced during viral infections activates the IFN-γ receptor (IFNGR) complex for STAT1 transcriptional activity leading to expression of Interferon Regulatory Factors (IRF). Simultaneous activation of TBK/IKKε via TLR3 during viral infections activates the transcription factor IRF3. Together these transcription factors contributes to expression of intracellular proteins (e.g. ISG49, ISG54) and secreted proteins (e.g. IFN-β, IP-10, IL-15) that are essential to innate antiviral immunity. Here we examined the role of IRF3 in expression of innate anti-viral proteins produced in response to IFN-γ plus TLR3 agonist. Wild-type (WT) and IRF3KO RAW264.7 cells, each with ISG54-promoter-luciferase reporter vectors, were stimulated with IFN-γ, poly I:C, or both together. ISG54 promoter activity was significantly reduced in IRF3KO RAW264.7 cells responding to IFN-γ, poly I:C, or IFN-γ plus poly I:C, compared with WT RAW264.7 cells. These data were confirmed with western blot and qRT-PCR. Primary macrophages and dendritic cells (DCs) from IRF3KO mice also showed decreased ISG54 in response to IFN-γ, poly I:C, or IFN-γ plus poly I:C compared with those from WT mice. Moreover, pharmacological inhibition of TBK/IKKε significantly reduced ISG54 promoter activity in response to IFN-γ, poly I:C, or IFN-γ plus poly I:C. Similarly, expression of ISG49 and IL-15, but not IP-10, was impaired in IRF3KO RAW264.7 cells responding to IFN-γ or poly I:C, which also had impaired STAT1 phosphorylation and IRF1 expression. These data show that IRF3 contributes to IFN-γ/IFNGR signaling for expression of innate anti-viral proteins in macrophages.
journal_name
Immunobiologyjournal_title
Immunobiologyauthors
Guinn ZP,Petro TMdoi
10.1016/j.imbio.2019.04.004subject
Has Abstractpub_date
2019-07-01 00:00:00pages
565-574issue
4eissn
0171-2985issn
1878-3279pii
S0171-2985(19)30092-0journal_volume
224pub_type
杂志文章相关文献
IMMUNOBIOLOGY文献大全abstract::This paper describes the characteristics of T suppressor inducer (Ts ind) cells which can interact with T suppressor effector (Ts eff) cells and thereby can account for suppression of delayed-type hypersensitivity (DTH) to alloantigens. Adoptive transfer of spleen cells from mice intravenously (i.v.) injected with all...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/s0171-2985(89)80007-5
更新日期:1989-03-01 00:00:00
abstract::Human recombinant erythropoietin (rHuEPO) has been purified to apparent homogeneity and compared to purified human urinary erythropoietin (EPO). Both the purified natural and recombinant EPO preparations were characterized in a competition radioimmunoassay (RIA), the exhypoxic polycythemic mouse bioassay, in vitro tis...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(86)80101-2
更新日期:1986-09-01 00:00:00
abstract::When sheep red blood cells (SRBC) were injected intraperitoneally (i.p.), intravenously (i.v.), or into the Peyer's patches, definite plaque-forming cell (PFC) and rosette-forming cell (RFC) responses were induced in the spleen but not in the mesenteric lymph nodes (MLN). When SRBC were injected directly into the sple...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/s0171-2985(82)80047-8
更新日期:1982-05-01 00:00:00
abstract::The distribution of MEL-14+ lymphocytes was investigated by both fluorocytometric analysis and complement-dependent-cellular-cytotoxicity (CDCC) tests in which rabbit anti-rat Ig was added with complement at a secondary step. When CDCC was employed to detect MEL-14+ cells, almost half of the thymocytes were found to b...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(11)80200-7
更新日期:1991-03-01 00:00:00
abstract::C1q binds to many non-self and altered-self-materials. These include microorganisms, immune complexes, apoptotic and necrotic cells and their breakdown products, and amyloids. C1q binding to amyloid fibrils found as extracellular deposits in tissues, and subsequent complement activation are involved in the pathology o...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2007.04.001
更新日期:2007-01-01 00:00:00
abstract::The collectins are a group of innate immune proteins structurally characterized by their content of a carbohydrate recognition domain and a collagen-like region. Collectin liver 1 (CL-L1) and collectin 11 (CL-11, alias collectin kidney 1, CL-K1) are the more recently described members of this group. Their genomic orga...
journal_title:Immunobiology
pub_type: 杂志文章,评审
doi:10.1016/j.imbio.2011.12.008
更新日期:2012-09-01 00:00:00
abstract::Mugwort pollen allergy is frequent in parts of Europe. As mugwort pollen contains only one major allergen, Art v 1, which harbors only one T cell epitope, we employed mugwort pollen allergy as a model to study allergen-specific T cell responses. However, after 2004, we noticed a drastic decrease in the T cell response...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2019.07.003
更新日期:2019-09-01 00:00:00
abstract::NK-92 cell line has been used as anti-tumor cytotoxic effector cells in immunotherapy. Leucine-rich repeats and calponin homology domain containing 1 (LRCH1) is a novel gene of which the function is unclear. In the present study, we investigated the role of LRCH1 in NK-92 cell cytotoxicity. LRCH1 was ablated in NK-92 ...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2020.151934
更新日期:2020-05-01 00:00:00
abstract::Phagocytosis Inhibitory Factor (PIF), a small (< 3000 D) molecule, was partially purified from human red blood cell membranes. This factor inhibits latex phagocytosis by monocytic cells. PIF is not toxic under the experimental conditions employed and the phagocytosis inhibitory activity is reversible since removal of ...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(11)80410-9
更新日期:1994-12-01 00:00:00
abstract::BCG infection of mice provides a convenient model to study natural and cellular immunity to mycobacteria and the mechanisms of granuloma formation and repair. We have used a range of macrophage (M phi) membrane molecules and secretory products to investigate resident M phi-pathogen interactions and T lymphocyte-depend...
journal_title:Immunobiology
pub_type: 杂志文章,评审
doi:10.1016/S0171-2985(11)80442-0
更新日期:1994-10-01 00:00:00
abstract::Interleukin (IL)-27, a member of the IL-6/IL-12 heterodimeric cytokine family, induces pro-inflammatory responses including early T helper (Th)1 differentiation and generation of cytotoxic T lymphocytes, and also anti-inflammatory responses including the differentiation to IL-10-producing regulatory T cells, inhibitio...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2012.07.028
更新日期:2013-04-01 00:00:00
abstract::Macrophages located in airways and the alveolar space are continually exposed to different signals from the respiratory mucosa. In this respect, epithelial cells represent an important source of cytokines and mediators modulating the state of activation and/or differentiation of mononuclear phagocytes. Many of the pro...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2011.04.009
更新日期:2011-10-01 00:00:00
abstract::Th2 cells play a central role in type I allergies. However, the source of interleukin-4 which may lead to a Th1/Th2 imbalance is unknown. Valpha24+CD161+ Natural killer T (NKT) cells secrete high amounts of interleukin-4 and/or interferon-gamma and are assumed to participate in the initiation of Th1/Th2 immune respons...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1078/0171-2985-00284
更新日期:2003-01-01 00:00:00
abstract::In mice, monoclonal antibody (mAb) to the alpha1 integrin abrogate gastro-intestinal damage during graft-versus-host-disease (GVHD), suggesting anti alpha1 mAb as candidates for treatment in humans as well. Our current data show that one such reagent, mAb 1B3.1, when immobilized to plastic wells via rabbit- anti murin...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/s0171-2985(00)80031-5
更新日期:2000-09-01 00:00:00
abstract::In contrast to murine spleen M phi, resident peritoneal M phi from health mice express very little Forssman glycolipid antigen (Fo). The following experiments suggest that Fo expression by peritoneal M phi may be associated with inflammation. Balb/c and CBA/J mice were given inflammatory stimuli by i.p. injection of l...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/s0171-2985(11)80302-5
更新日期:1990-06-01 00:00:00
abstract::The homotypic cell aggregation of leukocytes is an unique adhesive event which is caused by cellular activation. Anti-CD44 monoclonal antibody (mAb) induces homotypic cell aggregation of hematopoietic cell lines expressing CD44, but the mechanism of homotypic cell aggregation is poorly understood. We used four mAbs ag...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(11)80152-X
更新日期:1995-06-01 00:00:00
abstract::The leukocyte integrins CD11a/CD18 (LFA-1, alphaLbeta2) and CD49d (VLA-4, alpha4beta1, alpha4beta7) mediate leukocyte transendothelial migration during immune and inflammatory responses and provides co-stimulatory signals for the activation of T lymphocytes. Our previous studies demonstrate that the CD11a gene promote...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2005.05.008
更新日期:2005-01-01 00:00:00
abstract::The aim of the work was to justify the use of trifluoromethyl-dinitrobenzene sulfonate (CF3-DNBS) modification rather than trinitrobenzene-sulfonate (TNBS) modification, so as to be able to take advantage of the presence of fluorine atoms in the analogue, which allow the analysis of the hapten-carrier bonds by 19F-NMR...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(83)80012-6
更新日期:1983-02-01 00:00:00
abstract::Culture supernatants (CS) from Hodgkin derived cell lines have previously been shown to contain colony stimulating activity (CSF) for human cord blood cells, fetal bone marrow and fetal liver cells. In this study 3-day CS from four Hodgkin lines (L428, L538, L540, L591) and two sublines (L428KS, L428KSA) were examined...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/s0171-2985(84)80049-2
更新日期:1984-05-01 00:00:00
abstract::In order to evaluate the role of CD8+ T cells in cutaneous leishmaniasis, major histocompatibility complex (MHC) class II-deficient mice of C57BL/6 background lacking functional CD4+ T cells were infected with Leishmania major. In contrast to C57BL/6 wild-type mice which are resistant to infection with L. major, these...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(96)80043-X
更新日期:1996-07-01 00:00:00
abstract::Considering that imbalance of central tolerance in the thymus contributes to aggressive autoimmunity, we compared the expression of peripheral tissue autoantigens (PTA) genes, which are involved in self-representation in the thymic stroma, of two mouse strains; DBA-1/J (MHC-H2(q)) susceptible and DBA-2/J (MHC-H2(d)) r...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2010.09.007
更新日期:2011-05-01 00:00:00
abstract::The history of immunotherapy for tuberculosis is briefly reviewed, and the early appreciation of the importance of secreted antigens, common mycobacterial antigens and stress proteins is noted. The methods by which Mycobacterium vaccae strain NCTC 11659 was selected for special attention, and results of some of the pi...
journal_title:Immunobiology
pub_type: 历史文章,杂志文章
doi:10.1016/S0171-2985(11)80462-6
更新日期:1994-10-01 00:00:00
abstract::Several papers have demonstrated the importance of substances from natural sources which can exert powerful anti-inflammatory effects. In this respect, hydroxytyrosol, one of the major elements of the phenolic components of olive oil, has been extensively studied for its anti-inflammatory activities and safety profile...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2016.07.009
更新日期:2016-12-01 00:00:00
abstract::Regulatory T cells that express CD39 (CD39+ Treg) exhibit specific immunomodulatory properties. Ectonucleotidase CD39 hydrolyses ATP and ADP. ATP is a ligand of the P2X7 receptor and induces the shedding of CD62L and apoptosis. However, the role of ATP in CD39+ Treg cells has not been defined. Furthermore, NAD can act...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2015.07.018
更新日期:2016-01-01 00:00:00
abstract::Dendritic cells (DC) are efficient antigen presenting cells with the ability to activate naïve T cells. Murine DC represent a heterogeneous population that can be subdivided into distinct subsets, including the conventional DC (cDC) which are either CD4(-)CD8(-) (DN), CD4(+)CD8(-) (CD4+) or CD4(-)CD8(+) (CD8+) subsets...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2004.03.002
更新日期:2004-01-01 00:00:00
abstract::The goal of our work was to titer the IgG, IgM and IgA in Pentaglobin® (a preparation enriched in IgM), targeting specific surface antigens of Gram-positive and Gram-negative bacteria as well as a C. albicans strain. Lipopolysaccharides from Gram-negative bacteria, peptidoglycan and lipoteichoic acid from the other mi...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2019.12.006
更新日期:2020-03-01 00:00:00
abstract::CD163 and CD91 are scavenging receptors with highly increased expression during the differentiation of monocytes into the anti-inflammatory macrophage phenotype. In addition, CD91 is expressed in monocyte-derived dendritic cells (MoDCs), where the receptor is suggested to be important for internalization of CD91-targe...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2006.05.019
更新日期:2006-01-01 00:00:00
abstract::Hematopoietic cells express multiple receptors which bind the Fc domain of IgG. We utilized transfection of COS-1 cells, a cell line which lacks endogenous Fc receptors, to study the expression and function of Fc gamma RI, the high affinity Fc gamma receptor in the absence of other Fc gamma receptors. Fc gamma RI was ...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/s0171-2985(11)80640-6
更新日期:1992-08-01 00:00:00
abstract::In contrast to other pneumococcal serotypes, which are thought to be T-independent antigens, type 1 Streptococcus pneumoniae polysaccharide (Sp1) is a zwitterionic polysaccharide (ZPS). It has previously been shown to be processed and presented by antigen-presenting cells utilizing the MHC-II pathway, which leads to S...
journal_title:Immunobiology
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.imbio.2012.05.008
更新日期:2013-03-01 00:00:00
abstract::Surfactant protein D has multiple functions in innate immunity in the lung. The generation of SP-D knock-out mice has revealed a central role for this protein in the control of lung inflammation. Accumulating evidence in mouse models of infection and inflammation indicates that truncated recombinant forms of surfactan...
journal_title:Immunobiology
pub_type: 杂志文章,评审
doi:10.1078/0171-2985-00159
更新日期:2002-09-01 00:00:00