Growth temperature-dependent expression of structural variants of Listeria monocytogenes lipoteichoic acid.

Abstract:

:Investigating the expression of lipoteichoic acid (LTA) from Listeria monocytogenes, we found two distinct structural variants of LTA (LTA1 and LTA2) using NMR and MS technology. While both LTA consisted of a poly-glycerophosphate backbone (differing in length) bound via a disaccharide to a diacyl-glycerol moiety, one LTA type (LTA2) possessed a second diacyl-glycerol moiety linked to the disaccharide via a phosphodiester. As examined in vitro, LTA2 in contrast to LTA1 failed to activate the L-ficolin dependent pathway of complement. Most interestingly, growth temperature had a strong influence on the expression levels of LTA1 and LTA2 in the cell wall: while the amount of LTA1 was comparable, the expression of LTA2 was low when Listeria had grown at room temperature (ratio of LTA1 to LTA2 was 1:0.06), but increased when Listeria had been cultivated at 37°C (ratio of LTA1 to LTA2 was 1:0.68). The observed shift in LTA expression, probably accompanying the switch from the saprophytic to the virulent entity, indicates an important adaptation to the different structural requirements inside the host cells.

journal_name

Immunobiology

journal_title

Immunobiology

authors

Dehus O,Pfitzenmaier M,Stuebs G,Fischer N,Schwaeble W,Morath S,Hartung T,Geyer A,Hermann C

doi

10.1016/j.imbio.2010.03.008

subject

Has Abstract

pub_date

2011-01-01 00:00:00

pages

24-31

issue

1-2

eissn

0171-2985

issn

1878-3279

pii

S0171-2985(10)00031-8

journal_volume

216

pub_type

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