Abstract:
:Aggregatibacter actinomycetemcomitans is a Gram-negative bacterium frequently isolated from lesions of patients with localized aggressive periodontitis. Lipopolysaccharide (LPS), a major cell wall component of Gram-negative bacteria, stimulates innate immune cells via Toll-like receptor 4 (TLR4) to initiate inflammatory responses. In this study, we purified LPS from A. actinomycetemcomitans (AaLPS) and investigated its ability to induce the expression of chemokines, which play an important role in recruitment of leukocytes to the infection site. AaLPS induced the expression of chemokines, MCP-1, MIP-1α, and IP-10 in murine macrophages, leading to the infiltration of peripheral blood mononuclear cells in a transwell system. Although TLR4 was essential for the induction of all these chemokines by AaLPS, MCP-1 and MIP-1α expressions were MyD88-dependent, but IP-10 expression was MyD88-independent, as determined using macrophages from mice deficient in TLR4 or MyD88. Furthermore, the activation of ERK and JNK were necessary for the expression of MCP-1 and MIP-1α, whereas p38 MAP kinase and JNK activations were required for IP-10 expression. In addition, IFN-β/STAT1 signaling was exclusively involved in IP-10 expression but not in MCP-1 or MIP-1α expression. AaLPS also activated the transcription factors, NF-κB, AP-1, NF-IL6, and ISRE, all of which are involved in chemokine gene expression. These results suggest that AaLPS induces the expression of chemokines MCP-1, MIP-1α, and IP-10 through TLR4 in murine macrophages. Further, the induction of MCP-1 and MIP-1α requires MyD88, ERK, and JNK, whereas the induction of IP-10 requires JNK, p38 MAP kinase, and IFN-β/STAT1.
journal_name
Immunobiologyjournal_title
Immunobiologyauthors
Park OJ,Cho MK,Yun CH,Han SHdoi
10.1016/j.imbio.2015.05.008subject
Has Abstractpub_date
2015-09-01 00:00:00pages
1067-74issue
9eissn
0171-2985issn
1878-3279pii
S0171-2985(15)00076-5journal_volume
220pub_type
杂志文章相关文献
IMMUNOBIOLOGY文献大全abstract::Recently, it could be shown that Cyclosporin A (CsA) inhibited the activation of T and B lymphocytes by interfering with an early step of the activation, namely the stimulation of the plasma membrane-bound lysophosphatide acyltransferase. In this report, we compared three CsA-derivatives, Dihydro-Cyclosporin C (Dihydr...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(87)80025-6
更新日期:1987-09-01 00:00:00
abstract::Tumor necrosis factor (TNF) has been determined as an endogenous mediator for endotoxin-induced tumor necrosis. This macrophage product has been biochemically characterized and its protein structure defined by molecular cloning of the TNF gene. Experiments with antibodies to TNF demonstrated that TNF acts as an effect...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(86)80110-3
更新日期:1986-09-01 00:00:00
abstract::In the last decade new glucocorticoid (GC)-signalling mechanisms have emerged. The evolving field of non-genomic GC actions was precipitated from two major directions: (i) some rapid/acute clinical GC applications could not be explained based on the relatively slowly appearing genomic GC action and (ii) accumulating e...
journal_title:Immunobiology
pub_type: 杂志文章,评审
doi:10.1016/j.imbio.2009.10.003
更新日期:2010-07-01 00:00:00
abstract::Cytokines play critical roles in the pathogenesis of Behçet's disease (BD). They mediated many of the effectors and regulatory functions of immune and inflammatory responses. Many studies have linked Interleukin-6 (IL-6) and Interleukin-10 (IL-10) pathologically to BD. Thus, this study aimed to investigate the associa...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2014.03.004
更新日期:2014-08-01 00:00:00
abstract::Erythrocytes from paroxysmal nocturnal hemoglobinuria patients (PNH-E) are much more susceptible to lysis by acid-activated human serum than normal human erythrocytes. Acidification of normal human serum to pH 6.4 in the absence of erythrocytes generates this lytic activity independently of the alternative pathway of ...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(83)80003-5
更新日期:1983-03-01 00:00:00
abstract::Prior work demonstrated that a splice variant of SCN5A, a voltage-gated sodium channel gene, acts as a cytoplasmic sensor for viral dsRNA in human macrophages. Expression of this channel also polarizes macrophages to an anti-inflammatory phenotype in vitro and in vivo. Here we utilized global expression analysis of sp...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2018.10.005
更新日期:2019-01-01 00:00:00
abstract::Vaccines capable of inducing mucosal immunity in early postnatal life until adulthood, protecting early sexual initiation, should be considered as strategies to vaccination against HIV. The HIV-1 GAG protein as a chimera with the lysosome-associated membrane protein (LAMP/gag), encoded by a DNA vaccine, is targeted to...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2010.08.007
更新日期:2011-04-01 00:00:00
abstract::Gammaherpesviruses (gammaHV) establish a life-long latency in the host and are associated with a number of malignant human diseases. It is generally believed that T cells play a major role in controlling the initial acute infection and subsequently maintaining the virus in a quiescent state. However, the nature of the...
journal_title:Immunobiology
pub_type: 杂志文章,评审
doi:10.1078/0171-2985-00104
更新日期:2001-12-01 00:00:00
abstract::A glycoprotein from human erythrocyte membranes has been found to inhibit lysis of target cells by the attack-phase components C5-C9 from human complement. The inhibiting molecule was purified and identified as glycophorin A. Thus, glycophorin A may have a regulatory function in the lytic complement attack on isologou...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(83)80053-9
更新日期:1983-08-01 00:00:00
abstract::Activated macrophages have various characteristics in common with exudate and resident macrophages, but the ability to inhibit intracellular proliferation of the protozoa Toxoplasma gondii, the expression of Ia antigen and the capacity to produce H2O2 varies among these cells. Assessment of these features of macrophag...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(11)80575-9
更新日期:1991-12-01 00:00:00
abstract::L-alanine was shown earlier to play a significant role for the proliferation of lymphocytes in vitro. In the present work the effect of L-alanine and some other amino acids on thymocyte proliferation was studied in vivo by local administration into one thymus lobe of guinea pigs. Proliferating cells were pulse labelle...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(11)80487-0
更新日期:1993-06-01 00:00:00
abstract::The immunoglobulin production capacities of peripheral blood lymphocytes obtained from patients with various chronic inflammatory liver diseases and from normal individuals were compared. Using a reverse hemolytic plaque assay, immunoglobulin-secreting cells (ISC) were counted immediately after isolation (immediate IS...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(88)80066-4
更新日期:1988-12-01 00:00:00
abstract::Human sera enhanced spontaneous cell-mediated cytotoxicity (SCMC), while anti-IgG (Fab') 2 treatment decreased this cytotoxic activity of human lymphocytes for an in vitro growing cell line (K--562). Trypsin treatment of the effector cells considerably decreased the cytotoxic potential. However, a significant cytotoxi...
journal_title:Immunobiology
pub_type: 杂志文章
doi:
更新日期:1979-09-01 00:00:00
abstract::High Mphi:T cell ratios suppress the immune response to the retroviral superantigen Mls by IFNgamma-triggered production of the arg- and trp-consuming enzymes iNOS and IDO. Attempts to reverse suppression by treatment with pro-inflammatory cytokines revealed that IL-6 improved the T cell response to Mls and the pro-he...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2009.01.015
更新日期:2010-01-01 00:00:00
abstract::Previous studies have demonstrated that methamphetamine (METH) alter inflammatory and anti-inflammatory cytokine production in the periphery. However, the effect of METH on lipopolysaccharide (LPS)-induced immune responses and its underlying mechanism of action remains unclear. The dopamine D3 receptor (D3R) plays an ...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2014.12.021
更新日期:2015-06-01 00:00:00
abstract::By fusion of C3H/HeJ splenic adherent mononuclear cells enriched for macrophages with HPRT-deficient C57L/J HH- hepatoma cells, we have generated six macrophage-hepatoma hybrid clones. The hybrid nature of isolated clones was demonstrated by karyotypic analysis. The hybrid clones were screened for macrophage propertie...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(87)80017-7
更新日期:1987-08-01 00:00:00
abstract::CD147 is a broadly expressed cell surface molecule of the immunoglobulin superfamily whose expression is up-regulated upon T cell activation. Engagement of CD147 by CD147 monoclonal antibodies (mAbs) has been shown to induce homotypic aggregation of U937 cells. To study intracellular signal transduction induced by the...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(01)80015-2
更新日期:2001-05-01 00:00:00
abstract::CD40 is essential for optimal B cell activation. It has been shown that CD40 stimulation can augment BCR-induced B cell responses, but the molecular mechanism(s) by which CD40 regulates BCR signaling is poorly understood. In this report, we attempted to characterize the signaling synergy between BCR- and CD40-mediated...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2010.09.016
更新日期:2011-05-01 00:00:00
abstract::Human T-lymphocyte clones showing proliferative and cytotoxic responses specific for HLA antigens were isolated. To obtain enriched numbers of functionally active colonies, the cells were first sensitized in vitro in a tertiary mixed lymphocyte culture (MLC), using 10 stimulating cells for each responding cell. To ass...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/s0171-2985(81)80062-9
更新日期:1981-01-01 00:00:00
abstract::Beside the lung, thoracic lymph nodes are most affected during silicosis. The mechanisms leading to enlargement of the lymph nodes and partial activation of lymph node cells are still unclear. The present study demonstrates an increase in iNOS mRNA expression in the lung draining lymph nodes of rats at 1, 2, and 8 mon...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1078/0171-2985-00127
更新日期:2002-07-01 00:00:00
abstract::C1r, the enzyme responsible for intrinsic activation of the C1 complex of complement, is a modular serine protease featuring an overall structural organization homologous to those of C1s and the mannan-binding lectin-associated serine proteases (MASPs). This review will initially summarize current information on the s...
journal_title:Immunobiology
pub_type: 杂志文章,评审
doi:10.1078/0171-2985-00139
更新日期:2002-09-01 00:00:00
abstract::Macrophages orchestrate the initiation and resolution of inflammation by producing pro- and anti-inflammatory products. An imbalance in these mediators may originate from a deficient or excessive immune response. Therefore, macrophages are valid therapeutic targets to restore homeostasis under inflammatory conditions....
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2016.08.010
更新日期:2017-02-01 00:00:00
abstract::Intestinal epithelial cells produce cytokines in response to bacterial peptidoglycan (PGN), which is detected by several classes of pattern-recognition receptors (PRRs) as peptidoglycan recognition proteins (PGlyRPs), Toll-like receptor 2 (TLR2) and NOD receptors. All types of PGlyRPs recognize bacterial peptidoglycan...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2011.10.013
更新日期:2012-04-01 00:00:00
abstract::We studied local events in the popliteal lymph nodes of CD4-deficient mice following foot pad injection with an MMTV strain which carries the gene for a V beta 14-specific superantigen. Injection of the V beta 14-specific MMTV induced vigorous expansion of V beta 14+ CD4+ T cells and B cells in their lymph nodes of CD...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(96)80053-2
更新日期:1996-08-01 00:00:00
abstract::Carboxypeptidase N (CPN) is a member of the carboxypeptidase family of enzymes that cleave carboxy-terminal lysine and arginine residues from a large number of biologically active peptides and proteins. These enzymes are best known for their roles in modulating the activity of kinins, complement anaphylatoxins and coa...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2013.08.007
更新日期:2014-02-01 00:00:00
abstract::The inability of spontaneous and some laboratory-induced tumors to stimulate the immune system has continuously raised the question of the validity of using immunological maneuvers in order to control tumor growth. In this project we suggest that a tumor which is nonimmunogenic still has an immunogenic potential that ...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(82)80101-0
更新日期:1982-10-01 00:00:00
abstract::In contrast to other pneumococcal serotypes, which are thought to be T-independent antigens, type 1 Streptococcus pneumoniae polysaccharide (Sp1) is a zwitterionic polysaccharide (ZPS). It has previously been shown to be processed and presented by antigen-presenting cells utilizing the MHC-II pathway, which leads to S...
journal_title:Immunobiology
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.imbio.2012.05.008
更新日期:2013-03-01 00:00:00
abstract::The macrophage population of the gastro-intestinal tract of the rat was studied by using a set of three monoclonal antibodies (ED1, ED2 and ED3) directed against rat macrophages, an anti-Ia monoclonal antibody and by performing an acid phosphatase reaction. The lamina propria of the whole gastro-intestinal tract compr...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(86)80053-5
更新日期:1986-08-01 00:00:00
abstract::This paper describes the characteristics of T suppressor inducer (Ts ind) cells which can interact with T suppressor effector (Ts eff) cells and thereby can account for suppression of delayed-type hypersensitivity (DTH) to alloantigens. Adoptive transfer of spleen cells from mice intravenously (i.v.) injected with all...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/s0171-2985(89)80007-5
更新日期:1989-03-01 00:00:00
abstract::Natural killer (NK) cells are the main population of leukocytes in decidua during the first trimester of pregnancy. NK cells can have contact with trophoblast cells during pregnancy, which raises the possibility of mutual influence. This research aimed to evaluate the proliferation and phenotype of peripheral blood NK...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2020.151910
更新日期:2020-05-01 00:00:00